FastQCFastQC Report
Thu 26 May 2016
SRR1660705_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660705_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28227857
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19019566.737868907299623No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT472700.1674586916038295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA337460.11954857217818554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2388600.086.462488
AGTGGTA2404600.085.960295
GTATCAA2420050.085.280359
TGGTATC2415300.085.2149057
GTGGTAT2434550.084.945746
AAGCAGT2472350.084.076631
GCAGTGG2488900.083.60623
CAGTGGT2522450.081.853884
AGCAGTG2574600.080.8640442
CAACGCA2365250.045.13722212-13
ATCAACG2381700.044.9137110-11
ACGCAGA2390450.044.728514-15
AGAGTAC2375300.044.26685718-19
TCAACGC2365600.043.8070912-13
GAGTACT2323500.043.64167420-21
AACGCAG2387250.043.52507814-15
CGCAGAG2404150.043.15906516-17
AGTACTT2425500.042.9711620-21
TATCAAC2417400.042.6259610-11
CAGAGTA2470650.041.40715418-19
Produced by FastQC (version 0.11.5)