##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660705_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28227857 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76598648632803 34.0 31.0 34.0 30.0 34.0 2 31.88979106702999 34.0 31.0 34.0 30.0 34.0 3 31.95156692199482 34.0 31.0 34.0 30.0 34.0 4 35.351167536380814 37.0 35.0 37.0 33.0 37.0 5 35.315334564717396 37.0 35.0 37.0 33.0 37.0 6 35.39572061740287 37.0 35.0 37.0 33.0 37.0 7 35.328901552817136 37.0 35.0 37.0 33.0 37.0 8 35.352046561664245 37.0 35.0 37.0 33.0 37.0 9 37.01623885227986 39.0 37.0 39.0 33.0 39.0 10-11 36.93418326088303 39.0 37.0 39.0 33.0 39.0 12-13 36.9839212909432 39.0 37.0 39.0 33.0 39.0 14-15 38.35340010047521 40.0 38.0 41.0 33.0 41.0 16-17 38.32878085644263 40.0 38.0 41.0 33.0 41.0 18-19 38.28574659422428 40.0 38.0 41.0 33.0 41.0 20-21 38.14945110781878 40.0 38.0 41.0 33.0 41.0 22-23 37.949199721395786 40.0 38.0 41.0 32.5 41.0 24-25 37.95345066400188 40.0 38.0 41.0 32.5 41.0 26-27 37.96379482509069 40.0 38.0 41.0 32.5 41.0 28-29 37.89460363569222 40.0 38.0 41.0 32.0 41.0 30-31 37.80934374508132 40.0 38.0 41.0 32.0 41.0 32-33 37.68988352534165 40.0 38.0 41.0 32.0 41.0 34-35 37.53837347624369 40.0 37.5 41.0 31.0 41.0 36-37 37.34095972995754 40.0 37.0 41.0 31.0 41.0 38-39 36.85736829047986 39.0 35.0 41.0 30.0 41.0 40-41 36.79484542521241 39.0 35.0 41.0 30.0 41.0 42-43 36.79266176670797 39.0 35.0 41.0 30.0 41.0 44-45 36.79479841491332 39.5 35.0 41.0 30.0 41.0 46-47 36.887033737630176 40.0 35.0 41.0 30.0 41.0 48-49 36.7835508023156 40.0 35.0 41.0 30.0 41.0 50-51 35.421500363984414 38.5 34.0 40.0 28.0 40.5 52-53 35.56774986496495 38.5 34.5 39.5 28.0 40.5 54-55 35.86864158692599 39.0 35.0 41.0 26.0 41.0 56-57 35.423030820228405 39.0 35.0 41.0 23.0 41.0 58-59 35.2285444481315 39.0 35.0 41.0 22.0 41.0 60-61 35.01851385671962 39.0 35.0 41.0 22.0 41.0 62-63 34.80481329135258 38.5 34.0 41.0 22.0 41.0 64-65 34.57194616296944 37.5 34.0 40.0 22.0 41.0 66-67 34.33179578598545 37.0 34.0 40.0 22.0 41.0 68-69 34.08853172948977 37.0 34.0 40.0 22.0 41.0 70-71 33.81824771536854 36.0 33.5 39.0 22.0 41.0 72-73 33.49261550389744 35.5 33.0 39.0 21.5 41.0 74-75 33.11584083410937 35.0 33.0 38.5 20.5 40.0 76-77 32.713314669973 35.0 33.0 37.0 20.0 39.5 78-79 32.33297276162338 35.0 32.5 37.0 19.5 39.0 80-81 32.00733132167986 35.0 32.0 36.5 19.0 39.0 82-83 31.69562728406907 35.0 32.0 36.0 18.0 37.5 84-85 31.413007388410676 35.0 32.0 35.5 17.5 37.0 86-87 31.1965094091273 35.0 32.0 35.0 17.0 37.0 88-89 30.991641749495898 35.0 31.0 35.0 17.0 36.0 90-91 30.819497845691934 35.0 31.0 35.0 15.0 36.0 92-93 30.664996053366714 35.0 31.0 35.0 15.0 36.0 94-95 30.484057946729713 35.0 31.0 35.0 14.0 35.5 96-97 30.34750735771405 35.0 31.0 35.0 12.0 35.0 98-99 30.24700284545157 34.5 30.0 35.0 11.0 35.0 100 30.17118734872435 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5776865663808977 1101 2 0.6012329144910851 1101 3 0.5903505603713946 1101 4 0.5176178905089159 1101 5 0.558145050722139 1101 6 0.5236404870803852 1101 7 0.5429710460549018 1101 8 0.5365896640894903 1101 9 0.638189801962568 1101 10-11 0.6572603524965359 1101 12-13 0.6381227519616743 1101 14-15 0.7720733698396813 1101 16-17 0.7316108624203395 1101 18-19 0.7502314659513445 1101 20-21 0.7932348186484006 1101 22-23 0.8382153808699613 1101 24-25 0.8247399150281396 1101 26-27 0.7772254404459886 1101 28-29 0.7801023269452543 1101 30-31 0.7269276061123477 1101 32-33 0.7569138925446239 1101 34-35 0.7549797981535917 1101 36-37 0.7147089775467776 1101 38-39 0.7405843075723411 1101 40-41 0.750508061747361 1101 42-43 0.7503791391738943 1101 44-45 0.7605784206317736 1101 46-47 0.7882874234887183 1101 48-49 0.817867155579421 1101 50-51 0.7520952888677073 1101 52-53 0.7941052417053029 1101 54-55 0.8484144896745462 1101 56-57 0.8621894613226928 1101 58-59 0.7571150404622244 1101 60-61 0.7484982324558942 1101 62-63 0.7016170291058472 1101 64-65 0.6679757137010327 1101 66-67 0.7028976272271521 1101 68-69 0.6790105989635293 1101 70-71 0.6021163099247246 1101 72-73 0.57899546982933 1101 74-75 0.5482749975094308 1101 76-77 0.5249427101163349 1101 78-79 0.5461922897341296 1101 80-81 0.5076855010400649 1101 82-83 0.5151048441278014 1101 84-85 0.4788154742782744 1101 86-87 0.4673277221357601 1101 88-89 0.4998600162111799 1101 90-91 0.45731449115609735 1101 92-93 0.4704060128677199 1101 94-95 0.5227567615074022 1101 96-97 0.4961204206472267 1101 98-99 0.4739312149318913 1101 100 0.4271991677505156 1102 1 0.5229763192668528 1102 2 0.5171160515597464 1102 3 0.5111251283289597 1102 4 0.46162907845457823 1102 5 0.4818075207442689 1102 6 0.43888349423129114 1102 7 0.44471867298644696 1102 8 0.48611476599152326 1102 9 0.5438548932267437 1102 10-11 0.5542593815052257 1102 12-13 0.5800361607406259 1102 14-15 0.6526324451451444 1102 16-17 0.6262260554023698 1102 18-19 0.5935371508734093 1102 20-21 0.6131370938232763 1102 22-23 0.643783486346301 1102 24-25 0.58662588990763 1102 26-27 0.6033767575852735 1102 28-29 0.6279665926201972 1102 30-31 0.600873042827871 1102 32-33 0.6075174289923098 1102 34-35 0.5835752539075543 1102 36-37 0.5936609196164966 1102 38-39 0.5982648029468791 1102 40-41 0.6019872450861783 1102 42-43 0.6126196845603218 1102 44-45 0.6285616199907267 1102 46-47 0.6559679843502266 1102 48-49 0.6718196213350325 1102 50-51 0.6320303016522573 1102 52-53 0.6642847760270243 1102 54-55 0.6844464189188884 1102 56-57 0.704361775579649 1102 58-59 0.5804060828538553 1102 60-61 0.5827222988220697 1102 62-63 0.5157742942464196 1102 64-65 0.5025194803350175 1102 66-67 0.5206161768300603 1102 68-69 0.5033344779718902 1102 70-71 0.5510130071207016 1102 72-73 0.48809207587668624 1102 74-75 0.48293369588068913 1102 76-77 0.5092482872997337 1102 78-79 0.4834092015645055 1102 80-81 0.4456222658320357 1102 82-83 0.4191495630830069 1102 84-85 0.3522242562661191 1102 86-87 0.36394454964746004 1102 88-89 0.3981609057866464 1102 90-91 0.36654523268084205 1102 92-93 0.37186976583842934 1102 94-95 0.40158280644494226 1102 96-97 0.3465408192055648 1102 98-99 0.2720167331740768 1102 100 0.2686850940437111 1103 1 0.4110907812974105 1103 2 0.4463540971320725 1103 3 0.43150983422986045 1103 4 0.4122149134926332 1103 5 0.4372487954316142 1103 6 0.40430929939313387 1103 7 0.4198032765163475 1103 8 0.4354017312373557 1103 9 0.5006753538787265 1103 10-11 0.478811437811423 1103 12-13 0.46864584905898 1103 14-15 0.5824697864034221 1103 16-17 0.5323552441409021 1103 18-19 0.5476005194769513 1103 20-21 0.595360969995447 1103 22-23 0.6160731221146065 1103 24-25 0.6264096864021056 1103 26-27 0.569886957602904 1103 28-29 0.5774888174832924 1103 30-31 0.5423757728185663 1103 32-33 0.492666640712244 1103 34-35 0.539025113042797 1103 36-37 0.5374008034480937 1103 38-39 0.5380702503533143 1103 40-41 0.5312235046650926 1103 42-43 0.5197871875780251 1103 44-45 0.5088863768571699 1103 46-47 0.5308422251083655 1103 48-49 0.5731419718308359 1103 50-51 0.4909494943559807 1103 52-53 0.5038914087880428 1103 54-55 0.5825601324868188 1103 56-57 0.6306549613383439 1103 58-59 0.5399048036543235 1103 60-61 0.5225852338039303 1103 62-63 0.508667923787975 1103 64-65 0.48710909214083387 1103 66-67 0.4200169541411114 1103 68-69 0.39437845584542686 1103 70-71 0.4350014788495429 1103 72-73 0.4169509798665416 1103 74-75 0.38345887527326994 1103 76-77 0.38263238664418253 1103 78-79 0.3766254292077136 1103 80-81 0.315402308333816 1103 82-83 0.3397097732520926 1103 84-85 0.35049411529624663 1103 86-87 0.2893073009688507 1103 88-89 0.28600316198200204 1103 90-91 0.2916965165424479 1103 92-93 0.2664333916834174 1103 94-95 0.3029070256641013 1103 96-97 0.26284334274125953 1103 98-99 0.27210034394469673 1103 100 0.2598206880261884 1104 1 0.3917146584208808 1104 2 0.4155321245144208 1104 3 0.4071185949682423 1104 4 0.3456794312014182 1104 5 0.38170187033328773 1104 6 0.2857020376843664 1104 7 0.32797823540991544 1104 8 0.32209321794019985 1104 9 0.3803093102775108 1104 10-11 0.36254655021542703 1104 12-13 0.3845456661758675 1104 14-15 0.46380230140383816 1104 16-17 0.45971839695388894 1104 18-19 0.4229884589366222 1104 20-21 0.3764403984032825 1104 22-23 0.4388721237350026 1104 24-25 0.46249914716277374 1104 26-27 0.4314390213847332 1104 28-29 0.4171759294745243 1104 30-31 0.3767036122047571 1104 32-33 0.3467363875249987 1104 34-35 0.34255184061063204 1104 36-37 0.38383101549415954 1104 38-39 0.39086343157013914 1104 40-41 0.34785784492090954 1104 42-43 0.3836121811204478 1104 44-45 0.4126193067243875 1104 46-47 0.3979434730492599 1104 48-49 0.46007046974774113 1104 50-51 0.406137475169146 1104 52-53 0.38148111988594025 1104 54-55 0.5004451118361075 1104 56-57 0.5236815470618978 1104 58-59 0.4563020066835648 1104 60-61 0.44684461204106896 1104 62-63 0.40328404895748093 1104 64-65 0.41904013910001225 1104 66-67 0.4142076152328613 1104 68-69 0.3955245671747889 1104 70-71 0.42378773499890343 1104 72-73 0.41652867297511165 1104 74-75 0.3841229535970214 1104 76-77 0.370530945713206 1104 78-79 0.34500737900992107 1104 80-81 0.31927031139910866 1104 82-83 0.3285794596154936 1104 84-85 0.3151557334280106 1104 86-87 0.2767055567448402 1104 88-89 0.27295693800967413 1104 90-91 0.25882270121475415 1104 92-93 0.26284568245853634 1104 94-95 0.3779737897682267 1104 96-97 0.3129276367138303 1104 98-99 0.3129009682091741 1104 100 0.2557892070142209 1105 1 0.2774046116296347 1105 2 0.3111088225133898 1105 3 0.29191552082999905 1105 4 0.2516542731567526 1105 5 0.26154126894935814 1105 6 0.23512435073602234 1105 7 0.2665409998861179 1105 8 0.25430260787363324 1105 9 0.2900643767974671 1105 10-11 0.30417697074037875 1105 12-13 0.2851552106321904 1105 14-15 0.32620419944483103 1105 16-17 0.3160933279538938 1105 18-19 0.31790232618944714 1105 20-21 0.3575900718431342 1105 22-23 0.3901151649800809 1105 24-25 0.4073597602229384 1105 26-27 0.3838640985730706 1105 28-29 0.3748039060498769 1105 30-31 0.36641187463603586 1105 32-33 0.34479839340248475 1105 34-35 0.3601507499916963 1105 36-37 0.36165529406218866 1105 38-39 0.34539462641428287 1105 40-41 0.38759272089638586 1105 42-43 0.3886442287149876 1105 44-45 0.40888176996510595 1105 46-47 0.4110573853950399 1105 48-49 0.4206610240936328 1105 50-51 0.3345635827478688 1105 52-53 0.44024953162325886 1105 54-55 0.5018000616365015 1105 56-57 0.485191408488447 1105 58-59 0.3952841498884254 1105 60-61 0.39402042441500384 1105 62-63 0.41399612246370054 1105 64-65 0.3956445869542051 1105 66-67 0.40534871302301667 1105 68-69 0.4205838952213128 1105 70-71 0.3799433988399983 1105 72-73 0.3415774788570829 1105 74-75 0.37093965716236 1105 76-77 0.35753097973620385 1105 78-79 0.34047617917288875 1105 80-81 0.3287464770772601 1105 82-83 0.3344312331872423 1105 84-85 0.3317327353514834 1105 86-87 0.26141370469623126 1105 88-89 0.25905775991825664 1105 90-91 0.24448236682361468 1105 92-93 0.26419906377737234 1105 94-95 0.32662347551199744 1105 96-97 0.30972847928585523 1105 98-99 0.3062395000947511 1105 100 0.30270483832732964 1106 1 0.22386947165855275 1106 2 0.2750131367362769 1106 3 0.24845993964821744 1106 4 0.2507552115888032 1106 5 0.27105670841934426 1106 6 0.22847953811926658 1106 7 0.24892743697238018 1106 8 0.26104554821732506 1106 9 0.2831374068109369 1106 10-11 0.27866216357531215 1106 12-13 0.2464108913712053 1106 14-15 0.31285001994813655 1106 16-17 0.3127183830496918 1106 18-19 0.29948559147147336 1106 20-21 0.3084629966241508 1106 22-23 0.28736131242410323 1106 24-25 0.2987470069646747 1106 26-27 0.3159471069310342 1106 28-29 0.3100962192485426 1106 30-31 0.2790659477625539 1106 32-33 0.309291579728054 1106 34-35 0.30201052553415764 1106 36-37 0.26541859786342314 1106 38-39 0.31751443936231993 1106 40-41 0.32334475350105407 1106 42-43 0.36058992089679975 1106 44-45 0.3950072680357337 1106 46-47 0.3906299317434261 1106 48-49 0.40830208370497445 1106 50-51 0.2977057393225735 1106 52-53 0.36691973414688306 1106 54-55 0.5111514484977562 1106 56-57 0.5408019849527008 1106 58-59 0.463012522189139 1106 60-61 0.43151930643726644 1106 62-63 0.4283646949282627 1106 64-65 0.3538069527918637 1106 66-67 0.3623142570896789 1106 68-69 0.3545319902688959 1106 70-71 0.3822288759444348 1106 72-73 0.35143330691083463 1106 74-75 0.3362021888528872 1106 76-77 0.3611334513470652 1106 78-79 0.4118492833791194 1106 80-81 0.3532456933216679 1106 82-83 0.371728496407183 1106 84-85 0.3843998079389941 1106 86-87 0.35646174661895813 1106 88-89 0.3585661093815702 1106 90-91 0.3573730844830081 1106 92-93 0.3158779364007245 1106 94-95 0.37137862533707633 1106 96-97 0.39785356269080907 1106 98-99 0.369363221581299 1106 100 0.3714985400135262 1107 1 0.20583565329809517 1107 2 0.24035696167039333 1107 3 0.22013821338694228 1107 4 0.2540493401144488 1107 5 0.24257438884828275 1107 6 0.21361642959686122 1107 7 0.2281545876113782 1107 8 0.22779468900198907 1107 9 0.25538367809190277 1107 10-11 0.2249622503418749 1107 12-13 0.2284118140001894 1107 14-15 0.23885567340300895 1107 16-17 0.23639231904208202 1107 18-19 0.20547975417404274 1107 20-21 0.23484651376347898 1107 22-23 0.277387556659356 1107 24-25 0.27529194222886844 1107 26-27 0.23437569264648772 1107 28-29 0.2456197291903095 1107 30-31 0.20870532480426363 1107 32-33 0.16306580605408527 1107 34-35 0.22938301648554926 1107 36-37 0.22692891836713613 1107 38-39 0.2737513295986531 1107 40-41 0.24794487633601392 1107 42-43 0.29133643646184737 1107 44-45 0.3095168578928593 1107 46-47 0.2839174805675313 1107 48-49 0.3181356516415903 1107 50-51 0.24190060337642905 1107 52-53 0.2807606676569705 1107 54-55 0.3829143588698045 1107 56-57 0.3871365852169433 1107 58-59 0.34759297386467125 1107 60-61 0.36915120835676873 1107 62-63 0.2899839984700847 1107 64-65 0.28642360365632413 1107 66-67 0.34055835235528775 1107 68-69 0.27412365782976167 1107 70-71 0.26594995923842646 1107 72-73 0.2467866238692764 1107 74-75 0.2636838909639607 1107 76-77 0.25867880003406896 1107 78-79 0.2800292684693133 1107 80-81 0.19954106259441318 1107 82-83 0.22752637529066178 1107 84-85 0.27349725396308955 1107 86-87 0.2013307224132994 1107 88-89 0.22332553355957074 1107 90-91 0.23207836582409058 1107 92-93 0.23747942625457696 1107 94-95 0.25483031354598396 1107 96-97 0.27708555842559335 1107 98-99 0.26956258724610294 1107 100 0.26546647310279425 1108 1 0.10151663072824846 1108 2 0.13925393840382583 1108 3 0.1479592222097672 1108 4 0.13255998681653836 1108 5 0.14397127712287983 1108 6 0.1133233052844318 1108 7 0.1265626484689335 1108 8 0.15393477301219605 1108 9 0.16500598633138708 1108 10-11 0.128176985068869 1108 12-13 0.13322925220415271 1108 14-15 0.1566566213926066 1108 16-17 0.11715295343492471 1108 18-19 0.1254706476161047 1108 20-21 0.09351236444113198 1108 22-23 0.09142368254862276 1108 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-1.4420737158048063 2316 96-97 -1.5791305681202914 2316 98-99 -1.452784800741096 2316 100 -1.267638085811079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14435.0 3 3622.0 4 2558.0 5 2160.0 6 3218.0 7 14921.0 8 29620.0 9 35415.0 10 37754.0 11 38982.0 12 42992.0 13 49575.0 14 57517.0 15 67384.0 16 78145.0 17 92076.0 18 104594.0 19 118855.0 20 137656.0 21 165789.0 22 212355.0 23 303668.0 24 460919.0 25 597706.0 26 591897.0 27 492878.0 28 421621.0 29 402915.0 30 440019.0 31 525851.0 32 649191.0 33 832892.0 34 1140665.0 35 1748317.0 36 3046944.0 37 5714461.0 38 7314583.0 39 2229721.0 40 3986.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.198458475215237 34.41298569301121 27.55576571979055 22.832790111983 2 20.377622207516495 34.483337562967975 27.33567229283942 17.80336793667611 3 26.80793057807754 25.31615397927921 26.39779226005734 21.47812318258591 4 18.636622069109965 27.439948309708374 24.591616495908827 29.331813125272838 5 21.277091792353563 33.78605399177419 25.87126539204354 19.065588823828712 6 29.154528586199035 26.824995889944415 25.495327302982236 18.525148220874314 7 20.57232411997821 25.603419645822395 33.657535800632196 20.166720433567203 8 28.06421967412654 26.16686181517021 25.66854578031346 20.100372730389793 9 27.27206327207108 24.886238934686247 27.412407340828782 20.429290452413895 10-11 20.044687357523088 30.2328358962875 29.731008555895432 19.991468190293975 12-13 19.946654202854504 26.364440860247097 29.63671832820322 24.052186608695184 14-15 19.927778446781634 34.208468425255795 25.78731392373104 20.076439204231527 16-17 23.999360211804873 26.093368100811237 25.85866970920236 24.048601978181534 18-19 19.98877807348815 30.102565953034034 25.885563740900945 24.023092232576875 20-21 23.947745862912022 30.20793951320076 25.851360528887724 19.992954094999497 22-23 23.928439450649456 26.297313591107933 29.742048803224304 20.032198155018303 24-25 20.06046282602988 30.010885429142935 25.74701051387037 24.181641230956817 26-27 20.1011737326624 26.083263031937502 33.7533428435602 20.0622203918399 28-29 20.070606837135998 26.197025568236672 33.75329897417375 19.97906862045358 30-31 20.018712744530486 26.12652057228983 33.757542187676506 20.097224495503177 32-33 20.129573429538773 26.25909384241622 33.53803852360833 20.073294204436678 34-35 20.16481786886266 26.384235635283552 33.40794201896029 20.043004476893497 36-37 20.08627115987158 26.321617015887956 33.48954786534102 20.10256395889945 38-39 20.12013105134679 26.341585954914944 33.50646644080089 20.03181655293738 40-41 20.117685424127572 26.366888645787647 33.49004533733028 20.0253805927545 42-43 20.097443648447534 26.394017479162034 33.43373609289853 20.074802779491904 44-45 20.122219824853772 26.44489399484845 33.41943417232229 20.01345200797549 46-47 20.107700389202112 26.532002119487835 33.35831266530134 20.00198482600871 48-49 20.152381702728725 26.551765054637933 33.25833072115139 20.037522521481957 50-51 20.156149975536465 26.61762670795962 33.195902536982764 20.030320779521162 52-53 20.268586351907057 26.761177106556293 32.886182933490815 20.084053608045835 54-55 20.62109154268511 27.09401710311025 31.962606690524975 20.322284663679664 56-57 21.121161840323595 27.730552720243463 30.617255067513057 20.531030371919893 58-59 21.185792059818965 28.344597311851828 29.866410020970168 20.60320060735904 60-61 21.240081910083454 28.669782812173857 29.48814070536378 20.60199457237891 62-63 21.218315826117447 28.89512679060099 29.317582442368327 20.568974940913236 64-65 21.259924120706756 28.952554285919586 29.289607222354174 20.497914371019476 66-67 21.22701424589765 28.710314544633135 29.610598226825797 20.452072982643422 68-69 21.20455634426614 28.488023648358645 29.9602269800882 20.347193027287013 70-71 21.21290914875238 28.30729435746341 30.21088379851406 20.26891269527015 72-73 21.272340897760273 28.200379920767855 30.28657308396025 20.240706097511627 74-75 21.414956648625015 28.28502129722754 30.136863921512557 20.163158132634887 76-77 21.651303083539673 28.430754640595364 29.792318096127445 20.12562417973752 78-79 21.857812497783403 28.662490477494256 29.369204039102918 20.110492985619423 80-81 22.07898094313069 28.90289835603454 28.941489860456354 20.076630840378417 82-83 22.210519286325265 29.11820763998344 28.54371599805838 20.12755707563291 84-85 22.313744880755834 29.208625938943722 28.321865731049872 20.155763449250575 86-87 22.438096464395745 29.272628468470003 28.165685258877232 20.12358980825702 88-89 22.629568500270924 29.31411974648753 27.963643434621837 20.092668318619708 90-91 22.827146110638356 29.298147222106774 27.790295448666395 20.084411218588478 92-93 23.005792441638025 29.318738045198927 27.699099693601976 19.976369819561075 94-95 23.197638474524382 29.351058490560582 27.517936258982008 19.933366775933028 96-97 23.319893544657255 29.311735529732246 27.43435667995357 19.93401424565693 98-99 23.429091874196217 29.32447376548807 27.37247330863501 19.873961051680705 100 23.49420020648034 29.308805150743844 27.261891807650667 19.93510283512515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 42716.0 1 32167.0 2 18058.5 3 13502.0 4 12337.0 5 12298.0 6 12430.0 7 12192.0 8 11788.0 9 11953.5 10 12715.0 11 16500.0 12 159054.0 13 196694.0 14 91254.0 15 92726.5 16 103978.5 17 114717.5 18 124145.5 19 133502.5 20 144814.0 21 161904.0 22 185630.0 23 213249.5 24 241193.0 25 272169.5 26 307580.0 27 351507.0 28 409928.0 29 466727.0 30 508921.0 31 546522.0 32 584599.5 33 616158.0 34 643821.5 35 680304.5 36 722183.0 37 760969.0 38 806014.0 39 836783.0 40 849390.5 41 876692.5 42 911019.5 43 912718.0 44 899169.0 45 904675.5 46 911367.5 47 912620.5 48 907742.5 49 893598.0 50 877953.5 51 863336.0 52 844779.0 53 802463.5 54 744863.5 55 693622.0 56 643280.5 57 586400.5 58 521916.0 59 449039.5 60 369003.5 61 296187.5 62 231767.5 63 173663.5 64 126964.0 65 91444.0 66 64909.5 67 46601.5 68 33945.5 69 25256.5 70 19248.5 71 15030.0 72 12344.0 73 9928.0 74 8055.0 75 7011.0 76 5690.0 77 3863.5 78 2695.0 79 2134.0 80 1667.5 81 1386.5 82 1217.0 83 1040.5 84 894.5 85 767.5 86 631.5 87 562.0 88 497.5 89 408.5 90 350.5 91 304.5 92 285.0 93 264.5 94 206.5 95 168.5 96 140.5 97 113.0 98 84.5 99 65.5 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40525215924113545 2 0.3854383986712133 3 0.16779169598315594 4 0.17450137996660534 5 0.2751891509157071 6 0.10176117868246251 7 0.1355788361829947 8 0.1414276684198875 9 0.13409448687514608 10-11 0.13067233548760007 12-13 0.13265264876465827 14-15 0.13880083068296684 16-17 0.1431334302139904 18-19 0.15498165517842888 20-21 0.13615450864725578 22-23 0.0984630182872189 24-25 0.09351223509457342 26-27 0.08815582422711013 28-29 0.09782357902691657 30-31 0.10235810674540402 32-33 0.10335357728360321 34-35 0.10414357703455845 36-37 0.10019357827978227 38-39 0.09944254712640778 40-41 0.10046104456317743 42-43 0.06309015948323672 44-45 0.06619524819046661 46-47 0.08457425584946104 48-49 0.08878286438818221 50-51 0.09821680760250416 52-53 0.10297451910713591 54-55 0.10525418206560988 56-57 0.105934361223383 58-59 0.10524709686604973 60-61 0.10319947419317023 62-63 0.10748601992705291 64-65 0.11437637649928581 66-67 0.12288570117100989 68-69 0.12800475785320864 70-71 0.12645664174931878 72-73 0.11328525576702476 74-75 0.11104810400591161 76-77 0.11283888819473614 78-79 0.11158126527281188 80-81 0.10606898001502559 82-83 0.10894911363622112 84-85 0.11481743017190431 86-87 0.12828462323583403 88-89 0.1360198898556132 90-91 0.1430200670210282 92-93 0.1423257174641348 94-95 0.132016752104136 96-97 0.12865836751263124 98-99 0.12393608200580017 100 0.11834054565318225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8227857E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.79206236751538 #Duplication Level Percentage of deduplicated Percentage of total 1 61.587594613031506 20.81171838228815 2 17.801569745942338 12.031035101891169 3 7.917066154371823 8.026019797688335 4 3.8387723544728956 5.188801392681677 5 2.126041094689764 3.592165663382859 6 1.318083699889257 2.672445995535792 7 0.9427849692787059 2.2301053936715474 8 0.6860244002639418 1.8545743455485166 9 0.504367447436973 1.5339254615941256 >10 2.8744989912905523 17.37865460918656 >50 0.2189141688077787 5.20442400347382 >100 0.18149129934112276 11.338810856876192 >500 0.001963675474805761 0.40546761396594544 >1k 7.437289007473568E-4 0.5902334671360636 >5k 4.1828403921902104E-5 0.09836324048914392 >10k+ 4.182840391939456E-5 7.043254674590048 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1901956 6.737868907299623 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 47270 0.1674586916038295 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33746 0.11954857217818554 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012753359208245954 7.085199560136641E-6 0.0 0.0 0.0 2 0.0013284749175256202 1.4170399120273283E-5 0.0 0.0 0.0 3 0.0014737215085084212 1.4170399120273283E-5 0.0 0.0 0.0 4 0.001558743903230061 2.1255598680409923E-5 0.0 3.5425997800683206E-6 0.0 5 0.0015835421016905393 2.1255598680409923E-5 0.0 3.5425997800683206E-6 0.0 6 0.001647308897731769 2.4798198460478244E-5 0.0 3.5425997800683206E-6 0.0 7 0.001714618293553067 3.5425997800683204E-5 0.0 3.5425997800683206E-6 0.0 8 0.0017535868911338186 3.5425997800683204E-5 0.0 3.5425997800683206E-6 0.0 9 0.0018279814865152534 3.896859758075153E-5 0.0 3.5425997800683206E-6 0.0 10-11 0.0019183177809069954 3.896859758075153E-5 0.0 3.5425997800683206E-6 0.0 12-13 0.0020653356717798307 4.2511197360819846E-5 0.0 3.5425997800683206E-6 3.5425997800683206E-6 14-15 0.0022282952616629733 4.959639692095649E-5 0.0 3.5425997800683206E-6 3.5425997800683206E-6 16-17 0.0023735418526457746 5.845289637112729E-5 0.0 3.5425997800683206E-6 3.5425997800683206E-6 18-19 0.002529416242968781 6.376679604122977E-5 0.0 3.5425997800683206E-6 8.856499450170801E-6 20-21 0.002628609036810694 6.730939582129809E-5 0.0 3.5425997800683206E-6 1.0627799340204961E-5 22-23 0.002738429629992812 7.439459538143473E-5 0.0 3.5425997800683206E-6 1.7712998900341602E-5 24-25 0.002919102218776296 7.439459538143473E-5 0.0 3.5425997800683206E-6 2.1255598680409923E-5 26-27 0.0033778688902951436 7.793719516150304E-5 0.0 7.085199560136641E-6 2.1255598680409923E-5 28-29 0.004392823727284717 8.147979494157137E-5 0.0 7.085199560136641E-6 2.1255598680409923E-5 30-31 0.006578607791586871 8.502239472163969E-5 0.0 7.085199560136641E-6 2.4798198460478244E-5 32-33 0.011449682489180811 9.210759428177633E-5 0.0 7.085199560136641E-6 3.0112098130580727E-5 34-35 0.020504567527035437 9.919279384191298E-5 0.0 7.085199560136641E-6 3.3654697910649045E-5 36-37 0.035596042590126484 1.0627799340204961E-4 0.0 7.085199560136641E-6 4.428249725085401E-5 38-39 0.05852020576694859 1.0627799340204961E-4 0.0 7.085199560136641E-6 5.136769681099065E-5 40-41 0.09083580096073181 1.0804929329208377E-4 0.0 7.085199560136641E-6 5.3138996701024806E-5 42-43 0.13381285019263062 1.0982059318211793E-4 0.0 7.085199560136641E-6 5.3138996701024806E-5 44-45 0.18917305695575826 1.0982059318211793E-4 0.0 7.085199560136641E-6 5.4910296591058965E-5 46-47 0.26056352772369507 1.204483925223229E-4 0.0 7.085199560136641E-6 5.668159648109313E-5 48-49 0.34945798400494943 1.4170399120273282E-4 0.0 7.085199560136641E-6 5.668159648109313E-5 50-51 0.45589008049743207 1.5410309043297193E-4 0.0 7.085199560136641E-6 6.908069571133225E-5 52-53 0.5783241001964832 1.6827348955324523E-4 0.0 7.085199560136641E-6 7.262329549140057E-5 54-55 0.7217462522925491 1.8067258878348434E-4 0.0 7.085199560136641E-6 7.439459538143473E-5 56-57 0.8903828583232514 1.8421518856355265E-4 0.0 7.085199560136641E-6 7.970849505153722E-5 58-59 1.0845793217671464 1.9307168801372348E-4 0.0 8.856499450170801E-6 8.856499450170801E-5 60-61 1.3063531531989836 1.9838558768382595E-4 0.0 1.0627799340204961E-5 9.210759428177634E-5 62-63 1.5498165517842888 1.9838558768382595E-4 0.0 1.0627799340204961E-5 9.565019406184466E-5 64-65 1.816235996944437 1.9838558768382595E-4 0.0 1.7712998900341602E-5 1.027353936219813E-4 66-67 2.110989155145571 2.1078468691406506E-4 0.0 1.7712998900341602E-5 1.027353936219813E-4 68-69 2.434212416479225 2.1609858658416754E-4 0.0 1.7712998900341602E-5 1.027353936219813E-4 70-71 2.7843116110443664 2.267263859243725E-4 0.0 1.7712998900341602E-5 1.027353936219813E-4 72-73 3.159665999441615 2.3558288537454332E-4 0.0 1.7712998900341602E-5 1.027353936219813E-4 74-75 3.5613011643073014 2.408967850446458E-4 0.0 1.7712998900341602E-5 1.027353936219813E-4 76-77 3.9861633846310047 2.4443938482471414E-4 0.0 1.7712998900341602E-5 1.027353936219813E-4 78-79 4.441249294978361 2.4443938482471414E-4 0.0 1.7712998900341602E-5 1.0627799340204961E-4 80-81 4.91714620773373 2.497532844948166E-4 0.0 1.7712998900341602E-5 1.0982059318211793E-4 82-83 5.418262888323403 2.6038108383502157E-4 0.0 1.7712998900341602E-5 1.1336319296218625E-4 84-85 5.945635547183054 2.621523837250557E-4 0.0 1.948429879037576E-5 1.1690579274225458E-4 86-87 6.495170710266812 2.6923758328519236E-4 0.0 2.1255598680409923E-5 1.1690579274225458E-4 88 6.919009119254076 2.727801830652607E-4 0.0 2.1255598680409923E-5 1.1690579274225458E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 238860 0.0 86.46248 8 AGTGGTA 240460 0.0 85.96029 5 GTATCAA 242005 0.0 85.28035 9 TGGTATC 241530 0.0 85.214905 7 GTGGTAT 243455 0.0 84.94574 6 AAGCAGT 247235 0.0 84.07663 1 GCAGTGG 248890 0.0 83.6062 3 CAGTGGT 252245 0.0 81.85388 4 AGCAGTG 257460 0.0 80.864044 2 CAACGCA 236525 0.0 45.137222 12-13 ATCAACG 238170 0.0 44.91371 10-11 ACGCAGA 239045 0.0 44.7285 14-15 AGAGTAC 237530 0.0 44.266857 18-19 TCAACGC 236560 0.0 43.80709 12-13 GAGTACT 232350 0.0 43.641674 20-21 AACGCAG 238725 0.0 43.525078 14-15 CGCAGAG 240415 0.0 43.159065 16-17 AGTACTT 242550 0.0 42.97116 20-21 TATCAAC 241740 0.0 42.62596 10-11 CAGAGTA 247065 0.0 41.407154 18-19 >>END_MODULE