FastQCFastQC Report
Thu 26 May 2016
SRR1660705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28227857
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20602317.298573887489936No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT515440.1825997630638415No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT463110.164061338414744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA292270.1035395637720568No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2675300.086.508698
AAGCAGT2579800.086.3178941
AGTGGTA2668200.085.987635
GCAGTGG2676450.085.593273
TGGTATC2702300.085.5916447
GTGGTAT2688400.085.3677446
GTATCAA2710350.085.283879
AGCAGTG2749700.082.966662
CAGTGGT2779650.082.442744
CAACGCA2643050.045.0365312-13
ATCAACG2655250.044.89505410-11
ACGCAGA2671900.044.66639714-15
AGAGTAC2686550.044.3262518-19
TCAACGC2639200.043.73897612-13
GAGTACT2634450.043.5788420-21
AACGCAG2659550.043.5112714-15
AGTACTT2729900.043.25708820-21
CGCAGAG2688650.043.0475516-17
TATCAAC2703250.042.7009410-11
GTACTTT2755300.041.60627722-23
Produced by FastQC (version 0.11.5)