##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660705_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28227857 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.235447841470926 34.0 31.0 34.0 27.0 34.0 2 31.881189315930005 34.0 31.0 34.0 28.0 34.0 3 31.656668836036687 34.0 31.0 34.0 30.0 34.0 4 35.57804483705582 37.0 35.0 37.0 33.0 37.0 5 35.90843321900065 37.0 35.0 37.0 35.0 37.0 6 35.367013549771066 37.0 35.0 37.0 33.0 37.0 7 35.55440234092159 37.0 35.0 37.0 33.0 37.0 8 35.942643892520785 37.0 35.0 37.0 35.0 37.0 9 37.7550899453685 39.0 38.0 39.0 35.0 39.0 10-11 37.73391221303126 39.0 37.5 39.0 35.0 39.0 12-13 37.85542002001782 39.0 38.0 39.0 35.0 39.0 14-15 39.31791945807292 41.0 39.0 41.0 36.0 41.0 16-17 39.28464989389737 41.0 39.0 41.0 36.0 41.0 18-19 39.27235528364764 41.0 39.0 41.0 36.0 41.0 20-21 39.190440528305075 40.5 39.0 41.0 36.0 41.0 22-23 39.02632877869546 40.0 38.5 41.0 35.5 41.0 24-25 39.067572168159984 40.0 39.0 41.0 36.0 41.0 26-27 39.061348245458376 40.0 39.0 41.0 35.5 41.0 28-29 39.000172453757294 40.0 39.0 41.0 35.0 41.0 30-31 38.915123400972305 40.0 38.5 41.0 35.0 41.0 32-33 38.80234895266757 40.0 38.0 41.0 35.0 41.0 34-35 38.724185792779096 40.0 38.0 41.0 35.0 41.0 36-37 38.57840974254617 40.0 38.0 41.0 35.0 41.0 38-39 38.07646923037764 40.0 37.0 41.0 34.0 41.0 40-41 37.948720868183514 40.0 36.0 41.0 33.5 41.0 42-43 37.785496840939786 40.0 36.0 41.0 33.0 41.0 44-45 37.66550273370026 40.0 35.5 41.0 33.0 41.0 46-47 37.52127635831512 40.0 35.0 41.0 33.0 41.0 48-49 37.565604501964145 40.0 35.5 41.0 33.0 41.0 50-51 37.55309094133501 40.0 35.0 41.0 33.0 41.0 52-53 37.2562867595652 40.0 35.0 41.0 32.5 41.0 54-55 36.599666598849495 39.5 35.0 41.0 30.0 41.0 56-57 35.95880388652954 39.0 35.0 41.0 26.5 41.0 58-59 35.782903037945815 39.0 35.0 41.0 26.0 41.0 60-61 35.64573826840628 39.0 35.0 41.0 26.0 41.0 62-63 35.424060352863485 38.5 35.0 41.0 26.0 41.0 64-65 35.17800635733701 38.0 35.0 40.0 26.0 41.0 66-67 34.92650894469247 37.0 35.0 40.0 26.0 41.0 68-69 34.69621826056438 37.0 34.0 40.0 26.0 41.0 70-71 34.42816943206138 36.0 34.0 39.0 26.0 41.0 72-73 34.10312359170588 36.0 34.0 39.0 26.0 41.0 74-75 33.72530908031736 35.0 34.0 38.5 25.0 40.0 76-77 32.01505847574614 34.5 31.5 36.5 21.5 39.0 78-79 32.93803906190966 35.0 33.0 37.0 24.0 39.0 80-81 32.83101834475072 35.0 34.0 36.5 24.0 39.0 82-83 32.56932694182204 35.0 34.0 36.0 23.5 38.0 84-85 32.319507658693325 35.0 33.0 36.0 23.0 37.0 86-87 32.106717913442736 35.0 33.0 35.0 22.0 37.0 88-89 31.936177443438233 35.0 33.0 35.0 20.5 36.0 90-91 31.773956822156215 35.0 33.0 35.0 20.0 36.0 92-93 31.62922670679535 35.0 33.0 35.0 20.0 36.0 94-95 31.5212058428665 35.0 33.0 35.0 20.0 36.0 96-97 31.41913247257842 35.0 33.0 35.0 19.0 35.0 98-99 31.32564112110955 35.0 33.0 35.0 19.0 35.0 100 31.270415037174093 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.608498937366452 1101 2 1.2119760628985752 1101 3 1.5417209503004763 1101 4 0.969596998942869 1101 5 0.5898318138397585 1101 6 1.1338427502619695 1101 7 0.8315167288326393 1101 8 0.5046775578059339 1101 9 0.5302628333412756 1101 10-11 0.44255108610926897 1101 12-13 0.3970519598707867 1101 14-15 0.5383832474171584 1101 16-17 0.551966476050012 1101 18-19 0.5291723463089966 1101 20-21 0.5342816555275149 1101 22-23 0.5318071578329651 1101 24-25 0.5197798166892156 1101 26-27 0.49707440025188987 1101 28-29 0.5038612257217281 1101 30-31 0.4997917559687082 1101 32-33 0.5510576495028872 1101 34-35 0.5597787585219294 1101 36-37 0.5384343356009964 1101 38-39 0.5676894962935393 1101 40-41 0.5485403967184155 1101 42-43 0.5663934139219791 1101 44-45 0.5579575948862185 1101 46-47 0.5546041653642888 1101 48-49 0.5324054635087734 1101 50-51 0.5098846971956377 1101 52-53 0.5211819418085071 1101 54-55 0.5723323137678094 1101 56-57 0.5962148639738061 1101 58-59 0.5829673588835718 1101 60-61 0.577635055733964 1101 62-63 0.535788154805239 1101 64-65 0.49905379176613707 1101 66-67 0.5016833353560699 1101 68-69 0.4966216379256849 1101 70-71 0.49149377033671016 1101 72-73 0.49943741987310375 1101 74-75 0.48778839802024265 1101 76-77 0.3965587090896179 1101 78-79 0.5063297820587138 1101 80-81 0.5409566566561068 1101 82-83 0.5400614518723046 1101 84-85 0.48222676264043685 1101 86-87 0.4587023770047196 1101 88-89 0.42109872977969687 1101 90-91 0.4704812201964863 1101 92-93 0.4384055420821831 1101 94-95 0.4008142755562325 1101 96-97 0.3990051207487042 1101 98-99 0.43268322953017346 1101 100 0.4733825665550988 1102 1 1.507145436018309 1102 2 1.1353605333722676 1102 3 1.4874305911772545 1102 4 0.9067614975814209 1102 5 0.5001584126984469 1102 6 1.0799114832903172 1102 7 0.7804868706138279 1102 8 0.45760703255444213 1102 9 0.4533402759062142 1102 10-11 0.3963584719820048 1102 12-13 0.37261967525885353 1102 14-15 0.48020343433503143 1102 16-17 0.5017771573028114 1102 18-19 0.4470639027355858 1102 20-21 0.4176301846722552 1102 22-23 0.4499183343750772 1102 24-25 0.45079465561760657 1102 26-27 0.4613106627272856 1102 28-29 0.4659934751742938 1102 30-31 0.4601193801602932 1102 32-33 0.48143056391857186 1102 34-35 0.46389865631271476 1102 36-37 0.4372862550294343 1102 38-39 0.4691856752860559 1102 40-41 0.49529879730655324 1102 42-43 0.5315548373174437 1102 44-45 0.49240211727136085 1102 46-47 0.4907220215986783 1102 48-49 0.4912357763289137 1102 50-51 0.4655286988208118 1102 52-53 0.4451475908055116 1102 54-55 0.5230920760022428 1102 56-57 0.5239080545858386 1102 58-59 0.5118290538169887 1102 60-61 0.5501732272406912 1102 62-63 0.5079108592165795 1102 64-65 0.47376454084202635 1102 66-67 0.4636374870024511 1102 68-69 0.4461784653278187 1102 70-71 0.42031137175749933 1102 72-73 0.3820004700279611 1102 74-75 0.4123564222255709 1102 76-77 0.2547047181948727 1102 78-79 0.43104274079522753 1102 80-81 0.43643994558738086 1102 82-83 0.45238049189707397 1102 84-85 0.41931428784846503 1102 86-87 0.401777030391294 1102 88-89 0.39959383881582156 1102 90-91 0.39592502772414306 1102 92-93 0.3918674316966211 1102 94-95 0.378054952231448 1102 96-97 0.37855449134647756 1102 98-99 0.4118166686417588 1102 100 0.3973547091522285 1103 1 1.4644912317898182 1103 2 1.070325320589891 1103 3 1.408310460101891 1103 4 0.8618064277252344 1103 5 0.48990597347916065 1103 6 1.0387798603449667 1103 7 0.7491363014221051 1103 8 0.41975680021957373 1103 9 0.3943900838072878 1103 10-11 0.3305362492796249 1103 12-13 0.28986984592585685 1103 14-15 0.3544074993144335 1103 16-17 0.3810999913307853 1103 18-19 0.34105156760418964 1103 20-21 0.36945221427321684 1103 22-23 0.3654594991386588 1103 24-25 0.3449893837932976 1103 26-27 0.3304059592479405 1103 28-29 0.33704437608387394 1103 30-31 0.32141200724857555 1103 32-33 0.37147949040513595 1103 34-35 0.3645956706573088 1103 36-37 0.33279626272429397 1103 38-39 0.3595997181970034 1103 40-41 0.3833201978874641 1103 42-43 0.3891582735882366 1103 44-45 0.3738432416822022 1103 46-47 0.38187109569492605 1103 48-49 0.349973858594403 1103 50-51 0.3346376749803781 1103 52-53 0.31647736947663674 1103 54-55 0.34007919952505006 1103 56-57 0.361995133006225 1103 58-59 0.3186463609983221 1103 60-61 0.3171801524179827 1103 62-63 0.26856803276861996 1103 64-65 0.24553152291415614 1103 66-67 0.2766654962456201 1103 68-69 0.2084483082012838 1103 70-71 0.22073330418485426 1103 72-73 0.23554926327065573 1103 74-75 0.23099617363087077 1103 76-77 0.11153215249415638 1103 78-79 0.25141412230930626 1103 80-81 0.27490519229682064 1103 82-83 0.2966179038553918 1103 84-85 0.25335249973716145 1103 86-87 0.24384058664580976 1103 88-89 0.273369922349314 1103 90-91 0.28544972864567697 1103 92-93 0.2790070868431975 1103 94-95 0.26800514783422713 1103 96-97 0.27975539429024465 1103 98-99 0.2916582972575199 1103 100 0.29563612604163225 1104 1 1.4043350071235814 1104 2 1.0512051772478266 1104 3 1.3846160700553902 1104 4 0.8658886421399785 1104 5 0.48631500320309584 1104 6 1.012367629292143 1104 7 0.7425156694951482 1104 8 0.40975867849340375 1104 9 0.4012652885108068 1104 10-11 0.33074885301484613 1104 12-13 0.29544800456285003 1104 14-15 0.3500281977912749 1104 16-17 0.35811395658771517 1104 18-19 0.335549135616084 1104 20-21 0.3533092907550426 1104 22-23 0.3635918225756214 1104 24-25 0.3654484574711674 1104 26-27 0.36205544995041095 1104 28-29 0.31980248519167986 1104 30-31 0.327466749804465 1104 32-33 0.35903549017645275 1104 34-35 0.33358979842822833 1104 36-37 0.3413905653058862 1104 38-39 0.40503638570390876 1104 40-41 0.36618042982961185 1104 42-43 0.3914321610439657 1104 44-45 0.37483112711381494 1104 46-47 0.3923668790335526 1104 48-49 0.3024054442227211 1104 50-51 0.29083969332820203 1104 52-53 0.3403551512659462 1104 54-55 0.3478271126928618 1104 56-57 0.3712800666580378 1104 58-59 0.3581352667571096 1104 60-61 0.3216229214814703 1104 62-63 0.3052269066685298 1104 64-65 0.2273382802918107 1104 66-67 0.26213003955404446 1104 68-69 0.2349275992967108 1104 70-71 0.23141133522539548 1104 72-73 0.22062560402053322 1104 74-75 0.22233974687874536 1104 76-77 0.11396770789860255 1104 78-79 0.23795834314848463 1104 80-81 0.2826618369864491 1104 82-83 0.2870606655566945 1104 84-85 0.28117757395815346 1104 86-87 0.30589092530100714 1104 88-89 0.292763382256954 1104 90-91 0.28385393668778747 1104 92-93 0.2839480330248989 1104 94-95 0.2610814955186136 1104 96-97 0.28124088023735183 1104 98-99 0.29668766833119165 1104 100 0.3074876933173414 1105 1 1.3547459671220246 1105 2 1.000678945624255 1105 3 1.334093252143834 1105 4 0.8251610264723084 1105 5 0.42185868391646153 1105 6 0.9890481731094454 1105 7 0.6967093833149534 1105 8 0.3453813510475996 1105 9 0.3454554552370439 1105 10-11 0.2579845544224568 1105 12-13 0.23603067584456028 1105 14-15 0.3092531168333039 1105 16-17 0.2970229139725902 1105 18-19 0.2801247272613736 1105 20-21 0.3036096874260963 1105 22-23 0.3021884952121141 1105 24-25 0.29080605307668606 1105 26-27 0.3123501782618021 1105 28-29 0.2838166695716424 1105 30-31 0.30686106128778334 1105 32-33 0.36316191264263864 1105 34-35 0.3580861764055996 1105 36-37 0.32428543873926685 1105 38-39 0.36816413692147876 1105 40-41 0.36601539190395727 1105 42-43 0.3399628654433968 1105 44-45 0.3043567958957354 1105 46-47 0.34736324813071207 1105 48-49 0.355621230122928 1105 50-51 0.3169971924139574 1105 52-53 0.29074950633008 1105 54-55 0.38386013111970385 1105 56-57 0.39428075589120937 1105 58-59 0.3160381598459381 1105 60-61 0.2829626201347253 1105 62-63 0.27080300195403595 1105 64-65 0.2686392733282261 1105 66-67 0.2882324734536681 1105 68-69 0.24096636168987828 1105 70-71 0.28870521025143603 1105 72-73 0.27171212022186353 1105 74-75 0.2903729901284038 1105 76-77 0.1912326897683343 1105 78-79 0.3028812147265114 1105 80-81 0.35711406067460416 1105 82-83 0.33344315080323383 1105 84-85 0.32019077866125656 1105 86-87 0.3158557739104424 1105 88-89 0.29970353651878057 1105 90-91 0.33468601650788443 1105 92-93 0.32223033388391187 1105 94-95 0.31347464173212103 1105 96-97 0.28319464191474353 1105 98-99 0.28058411745015377 1105 100 0.2526290859734637 1106 1 1.321529121201909 1106 2 0.9706940103826405 1106 3 1.3000188995636996 1106 4 0.7905360787237967 1106 5 0.41056948726895826 1106 6 0.9478689297899763 1106 7 0.6767408995122821 1106 8 0.346774330562333 1106 9 0.31685689537707873 1106 10-11 0.22439764549003627 1106 12-13 0.19714034019157367 1106 14-15 0.2359393076766878 1106 16-17 0.24251617271072234 1106 18-19 0.20123184177589337 1106 20-21 0.22284496923746389 1106 22-23 0.2686223650721473 1106 24-25 0.23957101192667096 1106 26-27 0.22427734166082303 1106 28-29 0.22225301591465296 1106 30-31 0.22266696964452848 1106 32-33 0.2816754031304285 1106 34-35 0.27719160548753763 1106 36-37 0.24433867469085868 1106 38-39 0.2796210108214865 1106 40-41 0.3010683568103616 1106 42-43 0.3219134806088917 1106 44-45 0.32883757972896177 1106 46-47 0.32840430123696507 1106 48-49 0.30246698827914287 1106 50-51 0.29200170247201385 1106 52-53 0.2422272139008328 1106 54-55 0.3078587115121749 1106 56-57 0.31448949950106453 1106 58-59 0.2896358423225891 1106 60-61 0.28262631395993765 1106 62-63 0.27143018043789624 1106 64-65 0.2338773279653097 1106 66-67 0.27034449234678704 1106 68-69 0.2528170903463405 1106 70-71 0.22368648136367852 1106 72-73 0.1088320556421678 1106 74-75 0.19167064873969508 1106 76-77 0.09038426345669137 1106 78-79 0.2070993269965058 1106 80-81 0.23152026893448152 1106 82-83 0.23447292173725742 1106 84-85 0.23436932502652041 1106 86-87 0.23931255036803378 1106 88-89 0.21446240602076116 1106 90-91 0.2251525207437446 1106 92-93 0.25351403601047195 1106 94-95 0.23728695987251314 1106 96-97 0.2435650783670944 1106 98-99 0.2683966395771442 1106 100 0.25503580393214165 1107 1 1.2784653130413197 1107 2 0.8933233176994371 1107 3 1.2601343151768951 1107 4 0.783951819783475 1107 5 0.3681132206637159 1107 6 0.9257655521790298 1107 7 0.6461600667238017 1107 8 0.29329650395349205 1107 9 0.28292709418928297 1107 10-11 0.1969631440138926 1107 12-13 0.13300535985384698 1107 14-15 0.16845032288178885 1107 16-17 0.17643011988955948 1107 18-19 0.12425330218193409 1107 20-21 0.11131813744240304 1107 22-23 0.1564316390152669 1107 24-25 0.14463175490915603 1107 26-27 0.1349156994815104 1107 28-29 0.14956635585979683 1107 30-31 0.1547036670530062 1107 32-33 0.17220735789125285 1107 34-35 0.183220955723435 1107 36-37 0.1438102928354752 1107 38-39 0.17706140405704218 1107 40-41 0.20338314200837715 1107 42-43 0.2130198678048032 1107 44-45 0.2134684392197599 1107 46-47 0.24642853420826327 1107 48-49 0.22413447404630205 1107 50-51 0.19094299588589791 1107 52-53 0.17897227144776906 1107 54-55 0.2144577006068289 1107 56-57 0.21232201937333173 1107 58-59 0.2015000928914148 1107 60-61 0.21975181620247497 1107 62-63 0.17449865678982945 1107 64-65 0.13555121496761302 1107 66-67 0.16516157117440144 1107 68-69 0.16756294979290942 1107 70-71 0.15066344337913762 1107 72-73 0.15266228051308417 1107 74-75 0.17187451685184385 1107 76-77 0.07374194012966484 1107 78-79 0.20393781256175458 1107 80-81 0.24008510122743587 1107 82-83 0.22308612472353673 1107 84-85 0.2688843632482687 1107 86-87 0.18983337259418676 1107 88-89 0.22989099720559736 1107 90-91 0.24148439150997447 1107 92-93 0.22436455403049393 1107 94-95 0.2174976861179836 1107 96-97 0.23604666470425784 1107 98-99 0.24463677115535631 1107 100 0.23716329813836623 1108 1 1.2290261737387915 1108 2 0.8822749579814477 1108 3 1.1779615612907257 1108 4 0.7196912123819033 1108 5 0.3249909563301401 1108 6 0.8596280230600328 1108 7 0.5991370913893945 1108 8 0.23978085613666167 1108 9 0.17853341891785135 1108 10-11 0.11324601849963045 1108 12-13 0.10597270203577835 1108 14-15 0.09903232366109904 1108 16-17 0.12835304493118116 1108 18-19 0.09166246667120959 1108 20-21 0.09980682049712897 1108 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-0.7771619192171606 2316 92-93 -0.8281168709033473 2316 94-95 -0.8601543836490677 2316 96-97 -0.9712661848823885 2316 98-99 -1.1104215462559566 2316 100 -1.0383045405276938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 55.0 7 865.0 8 1004.0 9 366.0 10 379.0 11 704.0 12 965.0 13 1303.0 14 2344.0 15 4410.0 16 9060.0 17 15580.0 18 23975.0 19 36500.0 20 55361.0 21 83576.0 22 130023.0 23 224508.0 24 414814.0 25 604684.0 26 619531.0 27 490263.0 28 389822.0 29 375643.0 30 421379.0 31 507392.0 32 624703.0 33 795642.0 34 1090056.0 35 1695182.0 36 3063317.0 37 6148527.0 38 8057047.0 39 2334457.0 40 4420.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.312780036710116 34.53562907380449 27.900262315128543 21.251328574356855 2 20.769733223992215 35.48190024112133 26.120285598698423 17.628080936188034 3 27.72919555126426 25.06953803016772 26.428794925849264 20.77247149271875 4 19.126024023706144 27.176051482629532 24.27208346095657 29.425841032707755 5 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25.579787947748155 24.435370028655353 26-27 19.906701240368697 25.78313470270805 34.44993452864682 19.86022952827643 28-29 19.900809501053644 25.870052744286355 34.43730721404723 19.79183054061277 30-31 19.854349126756553 25.78741380722578 34.443903025919404 19.914334040098257 32-33 20.000981805002613 25.9237001101953 34.203558258328236 19.871759826473852 34-35 19.999368291731166 26.041069753134927 34.09746177987826 19.86210017525564 36-37 19.90737548139934 26.0063195014475 34.18138468103775 19.90492033611541 38-39 19.93012237202731 26.003048322463435 34.21909450584364 19.84773479966561 40-41 19.94256686652954 26.019758928082066 34.201307334685325 19.836366870703067 42-43 19.93742240250353 26.04806048777999 34.1335654880351 19.88095162168138 44-45 19.97456005014633 26.10095372436018 34.10563587719365 19.818850348299836 46-47 19.997847516304383 26.182868019384898 34.00440337778344 19.814881086527276 48-49 19.998346368059345 26.213039678164808 33.93452092411445 19.854093029661392 50-51 20.012328192168397 26.279400794432306 33.859998574599295 19.848272438800002 52-53 20.138742284835857 26.40763389899698 33.52579463197544 19.92782918419173 54-55 20.50736810655248 26.735148344197967 32.58027420300596 20.17720934624359 56-57 21.05590164561487 27.422381936198352 31.045654034915437 20.47606238327134 58-59 21.146782208396257 28.12307616320078 30.12559727852485 20.60454434987811 60-61 21.128836692181352 28.51432624976413 29.72704731872745 20.629789739327066 62-63 21.06970227814726 28.77839596091843 29.49365205630967 20.658249704624637 64-65 21.06708739503351 28.86904276688349 29.454069714773556 20.609800123309437 66-67 20.963048518285678 28.66315338508889 29.78000832023052 20.593789776394914 68-69 20.880528583971643 28.435892577364342 30.14927281282772 20.534306025836294 70-71 20.816408631351653 28.26061835036007 30.441943773324898 20.481029244963388 72-73 20.763373191444497 28.140388723111798 30.541384917104246 20.554853168339456 74-75 20.848534338950728 28.25025210797144 30.360773959863423 20.54043959321441 76-77 20.90872962287453 28.439665478191355 30.09117996937054 20.560424929563567 78-79 21.014405476574304 28.70222541479852 29.63663350863635 20.646735599990826 80-81 21.149461864245104 29.003516624023923 29.1332516259497 20.71376988578127 82-83 21.163091151201975 29.267554474591602 28.671029450697265 20.898324923509154 84-85 21.14164814942084 29.41091854057603 28.415734944356462 21.03169836564667 86-87 21.141188145649505 29.494983165621086 28.214865335514034 21.14896335321538 88-89 21.199319168666868 29.575122041657405 27.996095659531868 21.229463130143856 90-91 21.27283342461318 29.585586772273032 27.78832168678566 21.35325811632813 92-93 21.303577417879644 29.675792311698462 27.625814067671435 21.394816202750462 94-95 21.344950291688413 29.73623641718693 27.42585267553047 21.492960615594185 96-97 21.335716773282563 29.70555307462743 27.302900679341008 21.655829472748994 98-99 21.287928630549562 29.747461388432495 27.233698713539052 21.730911267478888 100 21.23151138468284 29.741385861542806 27.133335560598116 21.893767193176235 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 31209.0 1 24023.5 2 13546.5 3 9446.5 4 8529.5 5 8609.0 6 8798.5 7 8703.0 8 8496.5 9 8752.5 10 9977.5 11 22111.0 12 187731.5 13 220096.0 14 93900.0 15 95944.0 16 108654.0 17 119978.0 18 129356.5 19 139301.0 20 151581.0 21 169369.5 22 192778.0 23 219490.5 24 247516.0 25 278389.5 26 313566.5 27 357480.0 28 414462.5 29 470622.0 30 513452.0 31 550694.0 32 588376.0 33 618925.0 34 645001.5 35 679447.0 36 720004.0 37 757177.0 38 801318.5 39 831820.0 40 844651.5 41 873240.0 42 909739.0 43 914289.5 44 900008.5 45 905703.5 46 913911.5 47 914227.5 48 906558.5 49 892550.0 50 878727.5 51 864576.5 52 845244.5 53 800955.5 54 743061.5 55 693008.5 56 642932.5 57 584615.0 58 518989.5 59 444405.5 60 362724.5 61 290339.0 62 226138.5 63 167315.5 64 120908.0 65 85973.5 66 60156.5 67 42614.5 68 30277.5 69 21752.5 70 16017.0 71 12039.5 72 9393.5 73 7258.0 74 5735.5 75 5016.5 76 3966.5 77 2221.0 78 1117.5 79 714.5 80 472.0 81 345.5 82 269.0 83 185.0 84 146.0 85 112.0 86 67.5 87 49.0 88 34.0 89 19.5 90 14.0 91 10.0 92 10.0 93 8.0 94 1.5 95 2.0 96 3.0 97 2.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33079379706365947 2 0.006440446400164207 3 2.3706936024226 4 8.466813474363286E-4 5 2.1255598680409923E-5 6 0.008920266246212031 7 0.0076555581247276395 8 3.223765799862172E-4 9 0.0 10-11 7.61658952714689E-5 12-13 0.0 14-15 0.0 16-17 4.2511197360819846E-5 18-19 0.0 20-21 3.0643488097590975E-4 22-23 4.4282497250854005E-5 24-25 5.119056682198723E-4 26-27 0.003570940578308867 28-29 0.006258002511490689 30-31 0.005643361449648835 32-33 0.015339457047695827 34-35 0.009929907183531502 36-37 0.005035805587367118 38-39 0.005358182167353335 40-41 0.008282598285799733 42-43 0.018765151035021892 44-45 0.034154204479638677 46-47 0.03291960845628487 48-49 0.039602722941383756 50-51 0.03978339553016724 52-53 0.06172448726802038 54-55 0.03298691785210617 56-57 0.021810015545990612 58-59 0.0164164073808366 60-61 0.01757660880880897 62-63 0.02474860206355729 64-65 0.044852855815445 66-67 0.04780561273213195 68-69 0.05035097067411104 70-71 0.06064576563498958 72-73 0.06835446275641824 74-75 0.059267694320543 76-77 0.05945013820921652 78-79 0.05379083506055738 80-81 0.05163870569416587 82-83 0.04794377412355461 84-85 0.04983729370600114 86-87 0.058068524294989876 88-89 0.06457096619130527 90-91 0.060201169362591005 92-93 0.06505098846150453 94-95 0.07334244324675444 96-97 0.07243730900294698 98-99 0.04718742907051003 100 0.006752195180810219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8227857E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.55877469192587 #Duplication Level Percentage of deduplicated Percentage of total 1 58.87058591396912 19.167541427545853 2 19.334062936638365 12.589867980670464 3 8.362153048435577 8.16784371130245 4 4.016024007719163 5.230272832987735 5 2.2704170657532234 3.696099885028131 6 1.481183339964175 2.8935308785996683 7 0.9879168442095042 2.2515753361482975 8 0.7437569774312395 1.9372652682985214 9 0.5441458472794494 1.5945049837006828 >10 2.971015946395679 17.406678396958693 >50 0.23155702944023648 5.341742923923232 >100 0.18481770046691007 10.892837238935407 >500 0.0015219691566887694 0.2989893239412367 >1k 7.322243361285275E-4 0.5626021156866995 >5k 4.3659521841090464E-5 0.10914991855562543 >10k+ 6.548928274833228E-5 7.859497777717155 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2060231 7.298573887489936 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 51544 0.1825997630638415 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 46311 0.164061338414744 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29227 0.1035395637720568 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012540803221441855 1.0627799340204961E-5 0.0 0.0 3.5425997800683206E-6 2 0.001282421120384732 1.7712998900341602E-5 0.0 3.5425997800683206E-6 3.5425997800683206E-6 3 0.0013886991137867816 1.7712998900341602E-5 0.0 3.5425997800683206E-6 3.5425997800683206E-6 4 0.0014312103111476014 3.1883398020614886E-5 0.0 3.5425997800683206E-6 1.0627799340204961E-5 5 0.0014560085096080796 3.5425997800683204E-5 0.0 3.5425997800683206E-6 1.0627799340204961E-5 6 0.0015091475063091044 4.6053797140888164E-5 0.0 3.5425997800683206E-6 1.0627799340204961E-5 7 0.0015126901060891727 4.6053797140888164E-5 0.0 3.5425997800683206E-6 1.0627799340204961E-5 8 0.0015622865030101294 5.3138996701024806E-5 0.0 3.5425997800683206E-6 1.7712998900341602E-5 9 0.0016437662979517007 5.3138996701024806E-5 0.0 3.5425997800683206E-6 3.1883398020614886E-5 10-11 0.0017659859903640578 5.3138996701024806E-5 0.0 3.5425997800683206E-6 3.1883398020614886E-5 12-13 0.001921860380687064 5.3138996701024806E-5 0.0 3.5425997800683206E-6 3.1883398020614886E-5 14-15 0.0020281383740891135 5.3138996701024806E-5 0.0 3.5425997800683206E-6 3.5425997800683204E-5 16-17 0.0021291024678210604 5.4910296591058965E-5 0.0 5.313899670102481E-6 3.719729769071736E-5 18-19 0.0023398871547351255 6.376679604122977E-5 0.0 7.085199560136641E-6 5.136769681099065E-5 20-21 0.0024550216475873457 7.085199560136641E-5 0.0 7.085199560136641E-6 6.022419626116145E-5 22-23 0.002575470040109669 7.085199560136641E-5 0.0 7.085199560136641E-6 7.085199560136641E-5 24-25 0.002793339926583871 7.439459538143473E-5 0.0 7.085199560136641E-6 7.439459538143473E-5 26-27 0.00331233079436388 7.793719516150306E-5 0.0 7.085199560136641E-6 7.439459538143473E-5 28-29 0.004321971731683351 8.856499450170802E-5 0.0 7.085199560136641E-6 7.439459538143473E-5 30-31 0.006544953093676222 9.919279384191298E-5 0.0 7.085199560136641E-6 7.793719516150306E-5 32-33 0.011529390984232349 1.1159189307215209E-4 0.0 7.085199560136641E-6 7.970849505153722E-5 34-35 0.020745464312080082 1.1513449285222042E-4 0.0 7.085199560136641E-6 8.325109483160553E-5 36-37 0.036026468463404786 1.204483925223229E-4 0.0 7.085199560136641E-6 8.502239472163969E-5 38-39 0.059306662918123754 1.2221969241235707E-4 0.0 7.085199560136641E-6 8.856499450170801E-5 40-41 0.09213593508001688 1.2576229219242539E-4 0.0 1.0627799340204961E-5 9.565019406184466E-5 42-43 0.13554163888530396 1.4170399120273282E-4 0.0 1.0627799340204961E-5 1.0096409373194714E-4 44-45 0.19151117281060337 1.4170399120273282E-4 0.0 1.0627799340204961E-5 1.027353936219813E-4 46-47 0.2635889079358734 1.470178908728353E-4 0.0 1.0627799340204961E-5 1.1336319296218625E-4 48-49 0.35337078546203493 1.5764569021304024E-4 0.0 1.0627799340204961E-5 1.3107619186252786E-4 50-51 0.4604990028113009 1.6650218966321107E-4 0.0 1.0627799340204961E-5 1.3461879164259618E-4 52-53 0.5840684257398641 1.6650218966321107E-4 0.0 1.0627799340204961E-5 1.3639009153263034E-4 54-55 0.728787169355435 1.7004478944327938E-4 0.0 1.0627799340204961E-5 1.381613914226645E-4 56-57 0.8992995819696834 1.735873892233477E-4 0.0 1.0627799340204961E-5 1.381613914226645E-4 58-59 1.0953275694998736 1.735873892233477E-4 0.0 1.0627799340204961E-5 1.3993269131269866E-4 60-61 1.3193137544943636 1.7712998900341602E-4 0.0 1.0627799340204961E-5 1.4170399120273282E-4 62-63 1.5651294393336341 1.8067258878348434E-4 0.0 1.0627799340204961E-5 1.4170399120273282E-4 64-65 1.8341562379319125 1.8952908823365516E-4 0.0 1.0627799340204961E-5 1.4878919076286945E-4 66-67 2.132356345719053 1.9484298790375763E-4 0.0 1.0627799340204961E-5 1.558743903230061E-4 68-69 2.459495951109572 1.9484298790375763E-4 0.0 1.0627799340204961E-5 1.558743903230061E-4 70-71 2.812849023572707 1.9484298790375763E-4 0.0 1.0627799340204961E-5 1.5764569021304027E-4 72-73 3.193244531456993 1.9484298790375763E-4 0.0 1.0627799340204961E-5 1.7004478944327938E-4 74-75 3.600512429972987 1.9484298790375763E-4 0.0 1.0627799340204961E-5 1.7181608933331354E-4 76-77 4.031071150743041 1.9484298790375763E-4 0.0 1.4170399120273283E-5 1.7712998900341602E-4 78-79 4.491885444934768 1.9838558768382595E-4 0.0 1.4170399120273283E-5 1.7712998900341602E-4 80-81 4.973555024031757 2.0192818746389427E-4 0.0 1.4170399120273283E-5 1.7712998900341602E-4 82-83 5.48133002090807 2.2141248625427002E-4 0.0 1.4170399120273283E-5 1.824438886735185E-4 84-85 6.015314942257218 2.2495508603433833E-4 0.0 1.5941699010307443E-5 1.9838558768382595E-4 86-87 6.571949475300233 2.2672638592437252E-4 0.0 1.7712998900341602E-5 2.054707872439626E-4 88 7.001902411507895 2.2672638592437252E-4 0.0 1.7712998900341602E-5 2.054707872439626E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 267530 0.0 86.50869 8 AAGCAGT 257980 0.0 86.317894 1 AGTGGTA 266820 0.0 85.98763 5 GCAGTGG 267645 0.0 85.59327 3 TGGTATC 270230 0.0 85.591644 7 GTGGTAT 268840 0.0 85.367744 6 GTATCAA 271035 0.0 85.28387 9 AGCAGTG 274970 0.0 82.96666 2 CAGTGGT 277965 0.0 82.44274 4 CAACGCA 264305 0.0 45.03653 12-13 ATCAACG 265525 0.0 44.895054 10-11 ACGCAGA 267190 0.0 44.666397 14-15 AGAGTAC 268655 0.0 44.32625 18-19 TCAACGC 263920 0.0 43.738976 12-13 GAGTACT 263445 0.0 43.57884 20-21 AACGCAG 265955 0.0 43.51127 14-15 AGTACTT 272990 0.0 43.257088 20-21 CGCAGAG 268865 0.0 43.04755 16-17 TATCAAC 270325 0.0 42.70094 10-11 GTACTTT 275530 0.0 41.606277 22-23 >>END_MODULE