FastQCFastQC Report
Thu 26 May 2016
SRR1660704_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660704_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23984984
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18232917.60180202746852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA397090.16555775063264583No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT347030.14468635876513405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2286600.086.927148
AGTGGTA2298950.086.646755
GTGGTAT2308850.086.2023246
GTATCAA2306600.086.054819
TGGTATC2303300.086.050147
AAGCAGT2351700.085.236111
GCAGTGG2370050.084.597913
CAGTGGT2389450.083.23814
AGCAGTG2429650.082.600552
CAACGCA2272550.044.89368412-13
ATCAACG2279400.044.8211210-11
ACGCAGA2290450.044.56628814-15
AGAGTAC2272550.044.18527618-19
GAGTACT2215600.043.99769620-21
TCAACGC2266500.043.9951312-13
AACGCAG2283950.043.75341414-15
AGTACTT2298050.043.4204220-21
CGCAGAG2298450.043.3622516-17
TATCAAC2305150.042.9930310-11
GTACTTT2315150.042.1275522-23
Produced by FastQC (version 0.11.5)