##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660704_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23984984 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.759018684356846 34.0 31.0 34.0 30.0 34.0 2 31.877161102129566 34.0 31.0 34.0 30.0 34.0 3 31.94078836992345 34.0 31.0 34.0 30.0 34.0 4 35.33699738970016 37.0 35.0 37.0 33.0 37.0 5 35.30164714723178 37.0 35.0 37.0 33.0 37.0 6 35.38899250464374 37.0 35.0 37.0 33.0 37.0 7 35.31081050543957 37.0 35.0 37.0 33.0 37.0 8 35.342707295531234 37.0 35.0 37.0 33.0 37.0 9 37.011482058941546 39.0 37.0 39.0 33.0 39.0 10-11 36.908970566751265 39.0 37.0 39.0 33.0 39.0 12-13 36.97762735634929 39.0 37.0 39.0 33.0 39.0 14-15 38.35309842191265 40.0 38.0 41.0 33.0 41.0 16-17 38.326747539210366 40.0 38.0 41.0 33.0 41.0 18-19 38.27652934852907 40.0 38.0 41.0 33.0 41.0 20-21 38.13017878185785 40.0 38.0 41.0 33.0 41.0 22-23 37.91561541170926 40.0 37.5 41.0 32.5 41.0 24-25 37.92949793087209 40.0 38.0 41.0 32.0 41.0 26-27 37.955439349052725 40.0 38.0 41.0 32.5 41.0 28-29 37.89368677502557 40.0 38.0 41.0 32.0 41.0 30-31 37.80791196275136 40.0 38.0 41.0 32.0 41.0 32-33 37.684895495448316 40.0 38.0 41.0 32.0 41.0 34-35 37.5329683563683 40.0 37.5 41.0 31.0 41.0 36-37 37.324111535784226 39.5 37.0 41.0 31.0 41.0 38-39 36.795763257544806 39.0 35.0 41.0 30.0 41.0 40-41 36.72859160548116 39.0 35.0 41.0 30.0 41.0 42-43 36.71662317973612 39.0 35.0 41.0 30.0 41.0 44-45 36.711554008124416 39.0 35.0 41.0 30.0 41.0 46-47 36.79806344669649 40.0 35.0 41.0 30.0 41.0 48-49 36.688217907504125 40.0 35.0 41.0 30.0 41.0 50-51 35.30998446778201 38.0 34.0 40.0 27.5 40.0 52-53 35.4283015990338 38.5 34.5 39.5 27.0 40.5 54-55 35.65352311679674 39.0 35.0 41.0 24.5 41.0 56-57 35.14477866651902 39.0 35.0 41.0 21.0 41.0 58-59 34.93262697194211 39.0 35.0 41.0 20.0 41.0 60-61 34.72132901985675 39.0 34.0 41.0 20.0 41.0 62-63 34.50061525994764 38.0 34.0 41.0 20.0 41.0 64-65 34.256532920764094 37.5 34.0 40.0 20.0 41.0 66-67 34.00874305357052 37.0 34.0 40.0 20.0 41.0 68-69 33.76565610800491 36.5 33.0 40.0 20.0 41.0 70-71 33.49559165851434 36.0 33.0 39.0 20.0 41.0 72-73 33.17111647854341 35.5 33.0 39.0 20.0 41.0 74-75 32.79650090656721 35.0 33.0 38.5 19.5 40.0 76-77 32.40018152607482 35.0 32.0 37.0 18.5 39.5 78-79 32.02207272683609 35.0 32.0 37.0 17.5 39.0 80-81 31.696350829335554 35.0 32.0 36.0 16.5 39.0 82-83 31.383503966481694 35.0 31.5 36.0 15.0 37.5 84-85 31.10180306978733 35.0 31.0 35.5 14.5 37.0 86-87 30.892507016056378 35.0 31.0 35.0 13.0 37.0 88-89 30.692176467576544 35.0 31.0 35.0 12.0 36.0 90-91 30.51714583174206 35.0 31.0 35.0 11.0 36.0 92-93 30.363182939792665 35.0 31.0 35.0 10.0 36.0 94-95 30.187149989343332 35.0 30.0 35.0 10.0 35.5 96-97 30.051099617160467 34.5 30.0 35.0 10.0 35.0 98-99 29.947694670131945 34.0 30.0 35.0 10.0 35.0 100 29.866259531380134 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5296344891201734 1101 2 0.5312433268622918 1101 3 0.5409852550259124 1101 4 0.4755774885722559 1101 5 0.482071663040891 1101 6 0.4454183740780593 1101 7 0.5013765996264254 1101 8 0.5005972405670818 1101 9 0.559040454018195 1101 10-11 0.5960761056072457 1101 12-13 0.5729235363391822 1101 14-15 0.7060783472851639 1101 16-17 0.6798785979213662 1101 18-19 0.6666032398840187 1101 20-21 0.7248127968434304 1101 22-23 0.7626520576136997 1101 24-25 0.7427570040743703 1101 26-27 0.751962194660095 1101 28-29 0.7025946627750486 1101 30-31 0.6666530818474641 1101 32-33 0.6619654175312704 1101 34-35 0.6882226653183423 1101 36-37 0.6638087486855184 1101 38-39 0.6806597151144729 1101 40-41 0.650120965219493 1101 42-43 0.696389690426443 1101 44-45 0.6681212991922649 1101 46-47 0.7085209422400354 1101 48-49 0.7680536462257095 1101 50-51 0.6724955746568284 1101 52-53 0.7601445389914261 1101 54-55 0.8624792895793689 1101 56-57 0.7838883557218352 1101 58-59 0.686141063362065 1101 60-61 0.7112688424202531 1101 62-63 0.6489082712169534 1101 64-65 0.6248149409496264 1101 66-67 0.6247758325490196 1101 68-69 0.602326106178019 1101 70-71 0.5552150249663939 1101 72-73 0.5717049308299593 1101 74-75 0.5588815095655022 1101 76-77 0.5727081693794958 1101 78-79 0.582383873021648 1101 80-81 0.49235225834522467 1101 82-83 0.4785444682296891 1101 84-85 0.4869365168819719 1101 86-87 0.47148558734135193 1101 88-89 0.45436115486158357 1101 90-91 0.4288507827407102 1101 92-93 0.4252636453638452 1101 94-95 0.39583701564650653 1101 96-97 0.3992322522578178 1101 98-99 0.34510736862401714 1101 100 0.31551483321309703 1102 1 0.43411313664664775 1102 2 0.4798434078988727 1102 3 0.48225206851991587 1102 4 0.41385938078137485 1102 5 0.40632588963536165 1102 6 0.35748829357410017 1102 7 0.3773675666133727 1102 8 0.3763190574428421 1102 9 0.43269382882239427 1102 10-11 0.4536208887942408 1102 12-13 0.4596252381800099 1102 14-15 0.5596692133453516 1102 16-17 0.5001968962938932 1102 18-19 0.46121873022089943 1102 20-21 0.5142082786392379 1102 22-23 0.569235709070604 1102 24-25 0.5266772115286216 1102 26-27 0.4919646784461875 1102 28-29 0.5175269430891163 1102 30-31 0.46879674581659714 1102 32-33 0.5019438233894888 1102 34-35 0.48027850531081384 1102 36-37 0.5070702372189828 1102 38-39 0.49581825950875924 1102 40-41 0.47332268363546603 1102 42-43 0.5288636526361117 1102 44-45 0.5389173784426262 1102 46-47 0.5446764692318737 1102 48-49 0.5625802387384624 1102 50-51 0.5433042525450702 1102 52-53 0.5489144859903519 1102 54-55 0.581559288100415 1102 56-57 0.5842941771566572 1102 58-59 0.5032788891338811 1102 60-61 0.4619407474692707 1102 62-63 0.47045410037766544 1102 64-65 0.4742739173949886 1102 66-67 0.4617028397118119 1102 68-69 0.40038083825508153 1102 70-71 0.3641096613916872 1102 72-73 0.33183595628145923 1102 74-75 0.3380389921947682 1102 76-77 0.3520747482941289 1102 78-79 0.3417887083979778 1102 80-81 0.28596205142129705 1102 82-83 0.2998046725050365 1102 84-85 0.24951520053728515 1102 86-87 0.22533729108822342 1102 88-89 0.2609744532648932 1102 90-91 0.2197920112994609 1102 92-93 0.21218226171910715 1102 94-95 0.28613975804687897 1102 96-97 0.2862089303629247 1102 98-99 0.21581457872696674 1102 100 0.21391995271834574 1103 1 0.35139891547211377 1103 2 0.39395977064890175 1103 3 0.41151141909009326 1103 4 0.35846275044865905 1103 5 0.3877520840815478 1103 6 0.3543273593613492 1103 7 0.36156045562271544 1103 8 0.37799056320882585 1103 9 0.4506652707424408 1103 10-11 0.41893757492184847 1103 12-13 0.42367372777353296 1103 14-15 0.517046522021559 1103 16-17 0.5216833311996751 1103 18-19 0.4720181205348126 1103 20-21 0.5127238792023974 1103 22-23 0.5433762995824267 1103 24-25 0.5709862641222756 1103 26-27 0.5106206400789972 1103 28-29 0.48251430587060895 1103 30-31 0.4625263740965835 1103 32-33 0.4115690398417229 1103 34-35 0.4588680198086408 1103 36-37 0.4488209138044823 1103 38-39 0.45316961724704896 1103 40-41 0.4258420110177852 1103 42-43 0.48016597074654754 1103 44-45 0.5024609879419089 1103 46-47 0.49324494497017923 1103 48-49 0.5493961671619019 1103 50-51 0.45773512098290325 1103 52-53 0.47236305267843903 1103 54-55 0.5406947177215855 1103 56-57 0.5520398728539888 1103 58-59 0.4550886252187354 1103 60-61 0.4611881724959801 1103 62-63 0.4545803499964691 1103 64-65 0.3892933603176445 1103 66-67 0.3413517620130193 1103 68-69 0.358714489194476 1103 70-71 0.3556201538877275 1103 72-73 0.31923406106022156 1103 74-75 0.30013554026276523 1103 76-77 0.34841896084004986 1103 78-79 0.3491750862112539 1103 80-81 0.3001205176034567 1103 82-83 0.2947303035329156 1103 84-85 0.24105303090049546 1103 86-87 0.22752323407091168 1103 88-89 0.21274793550880133 1103 90-91 0.24495565203230285 1103 92-93 0.2294002359553673 1103 94-95 0.2847278774229949 1103 96-97 0.23917978224809033 1103 98-99 0.19168291576522378 1103 100 0.1860507959180815 1104 1 0.33641069786754585 1104 2 0.36711382956838534 1104 3 0.3441651376761179 1104 4 0.29877497085506377 1104 5 0.30671441540219746 1104 6 0.26062008502071876 1104 7 0.2891641386879584 1104 8 0.30554871279124285 1104 9 0.38683144640148726 1104 10-11 0.3540181362711792 1104 12-13 0.32555813544949785 1104 14-15 0.3798508269620555 1104 16-17 0.38186269970675113 1104 18-19 0.3787246671605189 1104 20-21 0.3385625832694359 1104 22-23 0.35837243810059505 1104 24-25 0.39214068171820315 1104 26-27 0.3610163376483939 1104 28-29 0.3161681290646925 1104 30-31 0.30370853901406036 1104 32-33 0.2957965828361111 1104 34-35 0.284386887459398 1104 36-37 0.28038760272423247 1104 38-39 0.29331497203204293 1104 40-41 0.28863250925299866 1104 42-43 0.3144896248673561 1104 44-45 0.2988982021730564 1104 46-47 0.35976159432764376 1104 48-49 0.395269932823183 1104 50-51 0.33641295488639855 1104 52-53 0.3426388194663943 1104 54-55 0.47760905848043933 1104 56-57 0.4605222628081549 1104 58-59 0.38180030137658605 1104 60-61 0.45214801998045573 1104 62-63 0.382402717524009 1104 64-65 0.3801867689571665 1104 66-67 0.36599416126312434 1104 68-69 0.37816874316443005 1104 70-71 0.4096415012227581 1104 72-73 0.3795218110059082 1104 74-75 0.343734363584403 1104 76-77 0.3828799305872792 1104 78-79 0.3520327190118806 1104 80-81 0.319544544732171 1104 82-83 0.36275686438818866 1104 84-85 0.3891080053823508 1104 86-87 0.3110859767582568 1104 88-89 0.2965326146036631 1104 90-91 0.24946248458169862 1104 92-93 0.24591618242602564 1104 94-95 0.31515245297424954 1104 96-97 0.2896135319513782 1104 98-99 0.31055921282315424 1104 100 0.22622345016864642 1105 1 0.32412785873772876 1105 2 0.3654098459070205 1105 3 0.35672903571412107 1105 4 0.31788932586765384 1105 5 0.3290813080751178 1105 6 0.3083282223938397 1105 7 0.34213718184420827 1105 8 0.35081123179337936 1105 9 0.34216777564229517 1105 10-11 0.36609723389352666 1105 12-13 0.37589530192423126 1105 14-15 0.4240207202833517 1105 16-17 0.39967492724239406 1105 18-19 0.3990429980605441 1105 20-21 0.3942903102871327 1105 22-23 0.47444218067661126 1105 24-25 0.5104913739708508 1105 26-27 0.4576025957099006 1105 28-29 0.44859979391657845 1105 30-31 0.46907437058881385 1105 32-33 0.42991224403095174 1105 34-35 0.43626926794697596 1105 36-37 0.4302751128244111 1105 38-39 0.4555992886219187 1105 40-41 0.4797301527242084 1105 42-43 0.5068471936053882 1105 44-45 0.49892642052197544 1105 46-47 0.49421677007534726 1105 48-49 0.5239029355858165 1105 50-51 0.4264502887792503 1105 52-53 0.44989030690540943 1105 54-55 0.5028919917885233 1105 56-57 0.5206935805045774 1105 58-59 0.4560342657113736 1105 60-61 0.427381056431841 1105 62-63 0.43375338568746 1105 64-65 0.43334355452675055 1105 66-67 0.39191184108997845 1105 68-69 0.37871243787024156 1105 70-71 0.3870885747780832 1105 72-73 0.40380790919123655 1105 74-75 0.42096994662113474 1105 76-77 0.3949410957539641 1105 78-79 0.44822306664516276 1105 80-81 0.3358924195579682 1105 82-83 0.33580429718768556 1105 84-85 0.2893054209741912 1105 86-87 0.1966654843070792 1105 88-89 0.24678391215136486 1105 90-91 0.2523076854422577 1105 92-93 0.23570227411704892 1105 94-95 0.2721826220768122 1105 96-97 0.2691698200769572 1105 98-99 0.26737487348691147 1105 100 0.3119938124681134 1106 1 0.1918325736787736 1106 2 0.2534447673189355 1106 3 0.23401385101164962 1106 4 0.23681914429530337 1106 5 0.2567520571654498 1106 6 0.20097235794673196 1106 7 0.21921056553797058 1106 8 0.24307516074315316 1106 9 0.24592635091355675 1106 10-11 0.22654829525653497 1106 12-13 0.2367710298243253 1106 14-15 0.3182601033471144 1106 16-17 0.286829677720128 1106 18-19 0.2673936314277867 1106 20-21 0.2742197666247037 1106 22-23 0.2802559588430782 1106 24-25 0.2594999838429217 1106 26-27 0.30812317163527325 1106 28-29 0.2825393058416523 1106 30-31 0.2473688164614316 1106 32-33 0.2769798748260328 1106 34-35 0.3031127885293614 1106 36-37 0.27342432491644075 1106 38-39 0.3260408095393288 1106 40-41 0.3394563505174304 1106 42-43 0.36058543841679125 1106 44-45 0.4070772323985139 1106 46-47 0.3954938714134286 1106 48-49 0.3872641056688906 1106 50-51 0.2937341722640383 1106 52-53 0.38062841042825113 1106 54-55 0.5299663307845464 1106 56-57 0.5347514868404559 1106 58-59 0.41084483223114177 1106 60-61 0.4361783665743957 1106 62-63 0.42374643722752126 1106 64-65 0.380441278730423 1106 66-67 0.4096667782964758 1106 68-69 0.4240794391997369 1106 70-71 0.41208005982587537 1106 72-73 0.36432336606515037 1106 74-75 0.3728337283970333 1106 76-77 0.3557071886159733 1106 78-79 0.36997110814142076 1106 80-81 0.32199027003169434 1106 82-83 0.34185198014318985 1106 84-85 0.3197466298693534 1106 86-87 0.2722971966580019 1106 88-89 0.3218319107727723 1106 90-91 0.30276459736118966 1106 92-93 0.26444045373114733 1106 94-95 0.32400701266194076 1106 96-97 0.39052230169977875 1106 98-99 0.3332907382314225 1106 100 0.3233406930418461 1107 1 0.2059406955992209 1107 2 0.24539158023434382 1107 3 0.22154434519991995 1107 4 0.1869779653088699 1107 5 0.21106964518214255 1107 6 0.16396265551180988 1107 7 0.19738251499856574 1107 8 0.205860967882046 1107 9 0.21044362274061257 1107 10-11 0.18170636004578 1107 12-13 0.19818688322209965 1107 14-15 0.24709630945354633 1107 16-17 0.2472467839531376 1107 18-19 0.2098435240809664 1107 20-21 0.22813435630954615 1107 22-23 0.24136085589552891 1107 24-25 0.23817735415983776 1107 26-27 0.22158199234202414 1107 28-29 0.24374538597729867 1107 30-31 0.21349005566872137 1107 32-33 0.17302408588736995 1107 34-35 0.23561157488081363 1107 36-37 0.22413124580786814 1107 38-39 0.2553794482839322 1107 40-41 0.2457485227565499 1107 42-43 0.2798069370106262 1107 44-45 0.23385021206774326 1107 46-47 0.24040762902987467 1107 48-49 0.31195018696331545 1107 50-51 0.23069365559816646 1107 52-53 0.24565325692331186 1107 54-55 0.3171198192715323 1107 56-57 0.34354499845407105 1107 58-59 0.28880753949455595 1107 60-61 0.3643602658975027 1107 62-63 0.2885996037231351 1107 64-65 0.2657874993093614 1107 66-67 0.3072164637465349 1107 68-69 0.27665728886665164 1107 70-71 0.2513097562894089 1107 72-73 0.25283017839856115 1107 74-75 0.2854126344572947 1107 76-77 0.2580014387357963 1107 78-79 0.2565932822272714 1107 80-81 0.20950640721371272 1107 82-83 0.28435340869486225 1107 84-85 0.2614166358277963 1107 86-87 0.2632456787426989 1107 88-89 0.23760262234269902 1107 90-91 0.2507834917307292 1107 92-93 0.25949611824676566 1107 94-95 0.2674320474686418 1107 96-97 0.26785623762523514 1107 98-99 0.29446360116718395 1107 100 0.2832404811730065 1108 1 0.1221893511919454 1108 2 0.16390693503206322 1108 3 0.18659447473851642 1108 4 0.17536857940637418 1108 5 0.17521145300459295 1108 6 0.11519841038705891 1108 7 0.14948388986707783 1108 8 0.18908103145546562 1108 9 0.18900599495101744 1108 10-11 0.15458991440925018 1108 12-13 0.18375404284554264 1108 14-15 0.23587771409686553 1108 16-17 0.17603276125905154 1108 18-19 0.18935209392358132 1108 20-21 0.17261631971797442 1108 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-1.4051168348147485 2316 90-91 -1.2750847454994982 2316 92-93 -1.1217072523464537 2316 94-95 -1.3541131173781977 2316 96-97 -1.5098730643188567 2316 98-99 -1.4095357221072184 2316 100 -1.197559182884504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12454.0 3 3049.0 4 2140.0 5 1830.0 6 3144.0 7 16374.0 8 31660.0 9 36168.0 10 37746.0 11 37196.0 12 39297.0 13 43992.0 14 50722.0 15 59213.0 16 70455.0 17 84390.0 18 93666.0 19 104782.0 20 120989.0 21 148330.0 22 192616.0 23 284889.0 24 432882.0 25 543472.0 26 528847.0 27 433466.0 28 368355.0 29 345552.0 30 374008.0 31 445064.0 32 549038.0 33 706299.0 34 971662.0 35 1492172.0 36 2599645.0 37 4835433.0 38 6040569.0 39 1839938.0 40 3480.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.437905876861475 35.85981268308611 26.827361372610785 20.874920067441625 2 20.577090980625233 35.32199615769361 25.867620406916153 18.233292454765003 3 27.989616763693668 24.993772021099744 25.995164206693307 21.021447008513277 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19.868946959631522 24-25 19.91904033091312 30.24682750110627 25.360777636488713 24.473354531491896 26-27 19.957408341501097 25.84807069468345 34.31315291048042 19.88136805333503 28-29 19.930131173853873 25.948699425282317 34.289405053893084 19.831764346970722 30-31 19.908967581752208 25.89002650660382 34.28261647710726 19.918389434536707 32-33 19.971680285135378 25.99092603848241 34.132777284061135 19.90461639232108 34-35 20.008417831278013 26.08557740316687 34.04129607973805 19.86470868581706 36-37 19.954057079601494 26.052243873633866 34.0956438091703 19.898055237594342 38-39 19.976078634259377 26.04905599437152 34.08643539243646 19.888429978932646 40-41 19.9934997894996 26.104476015736143 34.04322170465151 19.858802490112744 42-43 19.986824591617513 26.125312898218333 33.997680942890476 19.890181567273675 44-45 20.01250577440313 26.19750508236132 33.96996173483357 19.820027408401984 46-47 20.004789161014894 26.292383391680325 33.902311004911574 19.80051644239321 48-49 20.08945826969769 26.307322127284387 33.770962100376046 19.832257502641873 50-51 20.091623741317584 26.368482080696065 33.705353025365916 19.83454115262043 52-53 20.23029189260118 26.510984046236175 33.39379144857157 19.864932612591073 54-55 20.672576410711663 26.888229375449257 32.331258276307615 20.107935937531465 56-57 21.282428741594153 27.60054686594987 30.79563035633163 20.321394036124346 58-59 21.40277193632237 28.283175143451466 29.931824111332556 20.38222880889361 60-61 21.524705694000225 28.65317892549781 29.456397081918034 20.365718298583932 62-63 21.54799345165449 28.87310666888879 29.235464842919967 20.343435036536746 64-65 21.59516038979882 28.89907136772529 29.23707780448817 20.268690437987722 66-67 21.607461411680568 28.64172555341184 29.556710734565446 20.194102300342145 68-69 21.63576508146407 28.36648297196588 29.915919879459253 20.081832067110803 70-71 21.703860170698444 28.20077741853514 30.12973562651749 19.965626784248926 72-73 21.818889241896642 28.085334303268684 30.194446979593394 19.90132947524128 74-75 22.02069163491088 28.143538969329228 30.018376772994422 19.817392622765475 76-77 22.28780507473661 28.297865875139454 29.648834514246253 19.765494535877682 78-79 22.564502301104515 28.494295865358048 29.184067908670404 19.757133924867038 80-81 22.854458318202717 28.743504929783047 28.70723944649498 19.694797305519252 82-83 23.048585108810244 28.955118089317722 28.298994557333028 19.697302244539006 84-85 23.14917628885318 29.060383500548852 28.078182079404083 19.712258131193884 86-87 23.316789562474895 29.111538680065998 27.920709879685802 19.650961877773305 88-89 23.52279672243277 29.131425228804105 27.734777015012195 19.611001033750934 90-91 23.70356932116751 29.114751524738235 27.56421102801005 19.617468126084205 92-93 23.86289447207017 29.148001600377277 27.420228781301013 19.568875146251543 94-95 24.04686791940664 29.13730006144738 27.30373760247431 19.512094416671673 96-97 24.124256299292384 29.116393188001783 27.226146779482903 19.53320373322293 98-99 24.23731543247617 29.10297133957619 27.179312557592233 19.480400670355408 100 24.313076568684455 29.11133354212852 27.088099004148813 19.487490885038213 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 43418.0 1 29878.5 2 13708.5 3 10452.0 4 9670.5 5 9440.0 6 9447.0 7 9498.5 8 9422.5 9 9432.0 10 10141.5 11 13728.0 12 145432.0 13 179902.0 14 82879.5 15 85280.5 16 95552.5 17 104776.0 18 112684.0 19 120151.0 20 129020.0 21 142246.0 22 160855.0 23 182898.0 24 205431.5 25 230227.5 26 258708.5 27 293782.5 28 338301.0 29 380917.0 30 415260.5 31 447491.0 32 482840.5 33 514326.5 34 540506.5 35 571536.5 36 606423.5 37 640581.5 38 674335.5 39 696378.5 40 704499.5 41 720918.0 42 745363.0 43 752642.0 44 751037.0 45 757220.5 46 765916.0 47 772822.0 48 770694.5 49 764388.5 50 759235.5 51 748960.5 52 731057.5 53 697587.0 54 652329.0 55 606972.0 56 561169.5 57 509057.5 58 449633.0 59 387527.5 60 321629.5 61 259247.5 62 202836.5 63 151079.5 64 110189.5 65 80320.0 66 58309.5 67 42653.0 68 31545.0 69 23897.0 70 18431.0 71 14625.0 72 12241.0 73 10085.0 74 8235.0 75 7137.5 76 5858.5 77 4158.5 78 3051.5 79 2438.0 80 1987.5 81 1678.0 82 1361.5 83 1140.0 84 1010.0 85 851.0 86 720.5 87 618.0 88 540.5 89 489.0 90 421.0 91 359.5 92 298.5 93 243.5 94 220.0 95 204.5 96 174.0 97 150.0 98 119.5 99 83.5 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40716725097669443 2 0.38809281673900636 3 0.1693517911039674 4 0.17569742802413377 5 0.2769816315074465 6 0.10332297907724265 7 0.13685229058314152 8 0.14245579651001644 9 0.1355306303310438 10-11 0.1321597712969081 12-13 0.13382956603181392 14-15 0.14022523425489883 16-17 0.144596719347405 18-19 0.15670429465368832 20-21 0.13822398213815779 22-23 0.10005635192418723 24-25 0.09456958570412222 26-27 0.08910366586027324 28-29 0.09862003660290122 30-31 0.10309992285173089 32-33 0.10421937325453291 34-35 0.10477805613712313 36-37 0.10075053625218178 38-39 0.09996879714407982 40-41 0.10098610030342318 42-43 0.06390873556555218 44-45 0.06694396794260943 46-47 0.0852721019117628 48-49 0.08925792904427203 50-51 0.09846994269414562 52-53 0.10368987529864519 54-55 0.10590584509041155 56-57 0.10651455927592031 58-59 0.10626231812370607 60-61 0.10394211645085942 62-63 0.10821353893752858 64-65 0.11507199671260986 66-67 0.12373575066800127 68-69 0.1289431754467712 70-71 0.12748392911164752 72-73 0.11412765587002269 74-75 0.11153436666874574 76-77 0.11360441182699976 78-79 0.11225356664819955 80-81 0.10657709840456846 82-83 0.10937468209276272 84-85 0.11539720018158028 86-87 0.12905157660309466 88-89 0.1371253781115718 90-91 0.14409640631821977 92-93 0.14334593677444188 94-95 0.13328547561257492 96-97 0.1294288960126052 98-99 0.12478640802929032 100 0.11879515950479684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.3984984E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.880269580805916 #Duplication Level Percentage of deduplicated Percentage of total 1 61.716358886772205 22.76115953289926 2 18.509018823122613 13.652352077459462 3 7.874598695437059 8.712519681851441 4 3.667198906112194 5.409891370554172 5 2.0331590501377286 3.7491726934867367 6 1.314866383494302 2.9095576011605515 7 0.8659314962744268 2.2355050914778047 8 0.668093315013016 1.971156925025146 9 0.4904564541030486 1.627934962046994 >10 2.537055165888138 16.50605807445991 >50 0.20816461514293194 5.4004928868420174 >100 0.11357653597879946 6.233505008587086 >500 6.417893462871346E-4 0.15656362435317556 >1k 7.897184277685709E-4 0.6357823803169262 >5k 4.508237470556326E-5 0.10827593325259753 >10k+ 4.508237470535847E-5 7.9300721562267595 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1823291 7.60180202746852 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39709 0.16555775063264583 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 34703 0.14468635876513405 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006483223003192331 0.0 0.0 0.0 0.0 2 0.0065040693794083835 1.2507825729631507E-5 0.0 0.0 0.0 3 0.006545762131840488 2.084637621605251E-5 0.0 0.0 0.0 4 0.006587454884272593 2.5015651459263013E-5 0.0 0.0 0.0 5 0.006591624159515804 2.9184926702473514E-5 0.0 0.0 0.0 6 0.006591624159515804 3.752347718889452E-5 0.0 0.0 0.0 7 0.006591624159515804 5.0031302918526027E-5 0.0 0.0 0.0 8 0.006624978361461488 5.0031302918526027E-5 0.0 0.0 0.0 9 0.006666671113893592 5.420057816173653E-5 0.0 0.0 4.169275243210502E-6 10-11 0.006687517490109645 6.045449102655228E-5 0.0 0.0 6.253912864815753E-6 12-13 0.006737548793028171 9.172405535063104E-5 0.0 0.0 8.338550486421004E-6 14-15 0.006785495458325092 1.0840115632347305E-4 0.0 0.0 8.338550486421004E-6 16-17 0.006845949949351645 1.1673970680989406E-4 0.0 0.0 8.338550486421004E-6 18-19 0.00693558936708067 1.2299361967470983E-4 0.0 0.0 8.338550486421004E-6 20-21 0.006954351105675117 1.2924753253952558E-4 0.0 4.169275243210502E-6 8.338550486421004E-6 22-23 0.007056498349133775 1.3967072064755183E-4 0.0 8.338550486421004E-6 8.338550486421004E-6 24-25 0.007331670515185668 1.4800927113397283E-4 0.0 8.338550486421004E-6 8.338550486421004E-6 26-27 0.008165525563827769 1.6051709686360432E-4 0.0 8.338550486421004E-6 8.338550486421004E-6 28-29 0.010569112741538622 1.6677100972842008E-4 0.0 1.0423188108026255E-5 8.338550486421004E-6 30-31 0.016476975761167904 1.7719419783644632E-4 0.0 1.4592463351236759E-5 1.0423188108026255E-5 32-33 0.02888473888496236 1.9387129880928832E-4 0.0 1.667710097284201E-5 1.2507825729631507E-5 34-35 0.05170318229105343 2.1888695026855135E-4 0.0 1.667710097284201E-5 1.667710097284201E-5 36-37 0.08947681599454059 2.3556405124139335E-4 0.0 1.667710097284201E-5 1.667710097284201E-5 38-39 0.1460934891597176 2.459872393494196E-4 0.0 1.667710097284201E-5 1.667710097284201E-5 40-41 0.22316254203046373 2.501565145926301E-4 0.0 1.667710097284201E-5 1.667710097284201E-5 42-43 0.3224017160069817 2.584950650790511E-4 0.0 1.876173859444726E-5 1.667710097284201E-5 44-45 0.4483346747281549 2.584950650790511E-4 0.0 2.5015651459263013E-5 1.876173859444726E-5 46-47 0.6056310064663791 2.730875284302879E-4 0.0 2.5015651459263013E-5 2.084637621605251E-5 48-49 0.7974301754797919 2.939339046463404E-4 0.0 2.5015651459263013E-5 2.084637621605251E-5 50-51 1.0198109784021536 2.9810317988955093E-4 0.0 3.335420194568402E-5 2.293101383765776E-5 52-53 1.2676806455238827 3.0018781751115615E-4 0.0 3.752347718889452E-5 2.5015651459263013E-5 54-55 1.5442912115346834 3.231188313488139E-4 0.0 3.752347718889452E-5 2.5015651459263013E-5 56-57 1.8586816651618363 3.2937274421362966E-4 0.0 3.752347718889452E-5 2.5015651459263013E-5 58-59 2.2134744805333204 3.5647303329449796E-4 0.0 4.169275243210502E-5 2.5015651459263013E-5 60-61 2.6024616068119952 3.6689622140252415E-4 0.0 4.169275243210502E-5 2.5015651459263013E-5 62-63 3.016024526011775 3.7731940951055045E-4 0.0 4.169275243210502E-5 2.5015651459263013E-5 64-65 3.453168865987153 3.960811481049977E-4 0.0 4.169275243210502E-5 2.9184926702473514E-5 66-67 3.9204674891590505 4.1275824907783973E-4 4.169275243210502E-6 4.169275243210502E-5 3.335420194568402E-5 68-69 4.41706569410261 4.252660748074712E-4 4.169275243210502E-6 4.169275243210502E-5 3.335420194568402E-5 70-71 4.937710194011386 4.336046252938922E-4 4.169275243210502E-6 4.169275243210502E-5 3.752347718889452E-5 72-73 5.486209621820052 4.336046252938922E-4 4.169275243210502E-6 5.003130291852602E-5 3.960811481049977E-5 74-75 6.051319442197668 4.4402781340191846E-4 4.169275243210502E-6 5.836985340494703E-5 4.586202767531552E-5 76-77 6.637657127476091 4.690434648611815E-4 4.169275243210502E-6 5.836985340494703E-5 4.586202767531552E-5 78-79 7.245299809247319 4.81551290590813E-4 4.169275243210502E-6 5.836985340494703E-5 5.211594054013128E-5 80-81 7.870432600663815 4.857205658340235E-4 4.169275243210502E-6 5.836985340494703E-5 5.836985340494703E-5 82-83 8.511291898297703 5.003130291852603E-4 4.169275243210502E-6 6.253912864815753E-5 6.253912864815753E-5 84-85 9.166430963639584 5.169901301581023E-4 4.169275243210502E-6 6.462376626976279E-5 6.670840389136804E-5 86-87 9.834628199043202 5.3992114399576E-4 4.169275243210502E-6 6.670840389136804E-5 6.670840389136804E-5 88 10.34586056009043 5.545136073469968E-4 4.169275243210502E-6 6.670840389136804E-5 6.670840389136804E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 228660 0.0 86.92714 8 AGTGGTA 229895 0.0 86.64675 5 GTGGTAT 230885 0.0 86.202324 6 GTATCAA 230660 0.0 86.05481 9 TGGTATC 230330 0.0 86.05014 7 AAGCAGT 235170 0.0 85.23611 1 GCAGTGG 237005 0.0 84.59791 3 CAGTGGT 238945 0.0 83.2381 4 AGCAGTG 242965 0.0 82.60055 2 CAACGCA 227255 0.0 44.893684 12-13 ATCAACG 227940 0.0 44.82112 10-11 ACGCAGA 229045 0.0 44.566288 14-15 AGAGTAC 227255 0.0 44.185276 18-19 GAGTACT 221560 0.0 43.997696 20-21 TCAACGC 226650 0.0 43.99513 12-13 AACGCAG 228395 0.0 43.753414 14-15 AGTACTT 229805 0.0 43.42042 20-21 CGCAGAG 229845 0.0 43.36225 16-17 TATCAAC 230515 0.0 42.99303 10-11 GTACTTT 231515 0.0 42.12755 22-23 >>END_MODULE