FastQCFastQC Report
Thu 26 May 2016
SRR1660704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23984984
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15724856.556122780819866No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT367930.15340014402344399No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT344240.1435231309722783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA338320.14105492002829773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2053800.085.3922968
AAGCAGT1991850.085.2834551
AGTGGTA2050800.084.9042365
GTGGTAT2055200.084.669926
TGGTATC2072950.084.5561457
GCAGTGG2065550.084.3717653
GTATCAA2076850.084.310879
AGCAGTG2123500.081.762422
CAGTGGT2139650.081.210614
CAACGCA2024050.044.47146212-13
ATCAACG2033600.044.34691210-11
ACGCAGA2051100.044.0018214-15
AGAGTAC2064300.043.6206318-19
GAGTACT1997950.043.49890520-21
TCAACGC2020500.043.25909812-13
AACGCAG2035350.043.02663414-15
AGTACTT2089100.042.76603320-21
CGCAGAG2060900.042.55137616-17
TATCAAC2075650.042.1346210-11
GTACTTT2101750.041.3275522-23
Produced by FastQC (version 0.11.5)