##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660704_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23984984 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22165122144755 34.0 31.0 34.0 27.0 34.0 2 31.87036772674103 34.0 31.0 34.0 28.0 34.0 3 31.637119416047973 34.0 31.0 34.0 30.0 34.0 4 35.56335672352335 37.0 35.0 37.0 33.0 37.0 5 35.89907247801374 37.0 35.0 37.0 33.0 37.0 6 35.362626466625954 37.0 35.0 37.0 33.0 37.0 7 35.56177360802075 37.0 35.0 37.0 33.0 37.0 8 35.94108363799617 37.0 35.0 37.0 35.0 37.0 9 37.7552712980755 39.0 38.0 39.0 35.0 39.0 10-11 37.747577213309796 39.0 37.5 39.0 35.0 39.0 12-13 37.84968153407982 39.0 38.0 39.0 35.0 39.0 14-15 39.30750097644426 41.0 39.0 41.0 36.0 41.0 16-17 39.27229242679503 41.0 39.0 41.0 36.0 41.0 18-19 39.26060844985346 41.0 39.0 41.0 36.0 41.0 20-21 39.18359876746217 40.5 39.0 41.0 36.0 41.0 22-23 39.0336853257855 40.0 38.5 41.0 35.5 41.0 24-25 39.06372245234769 40.0 39.0 41.0 36.0 41.0 26-27 39.041183162765506 40.0 39.0 41.0 35.5 41.0 28-29 38.9714646672268 40.0 39.0 41.0 35.0 41.0 30-31 38.88126489890508 40.0 38.0 41.0 35.0 41.0 32-33 38.763254793082204 40.0 38.0 41.0 35.0 41.0 34-35 38.6791609908933 40.0 38.0 41.0 35.0 41.0 36-37 38.537733942203175 40.0 38.0 41.0 34.5 41.0 38-39 38.07409125226016 40.0 37.0 41.0 34.0 41.0 40-41 37.94319900317632 40.0 36.5 41.0 33.0 41.0 42-43 37.77810187407255 40.0 36.0 41.0 33.0 41.0 44-45 37.654372252239156 40.0 36.0 41.0 33.0 41.0 46-47 37.50652368581943 40.0 35.0 41.0 33.0 41.0 48-49 37.54843238586275 40.0 35.5 41.0 33.0 41.0 50-51 37.53886773491281 40.0 35.5 41.0 33.0 41.0 52-53 37.25509748099061 40.0 35.0 41.0 32.5 41.0 54-55 36.645068347762916 39.5 35.0 41.0 30.0 41.0 56-57 36.05654143859341 39.0 35.0 41.0 27.5 41.0 58-59 35.893281625703814 39.0 35.0 41.0 27.0 41.0 60-61 35.75399078857005 39.0 35.0 41.0 27.0 41.0 62-63 35.52336686987158 39.0 35.0 41.0 26.5 41.0 64-65 35.264649978503215 38.0 35.0 40.0 26.0 41.0 66-67 34.9963417111306 37.0 35.0 40.0 26.0 41.0 68-69 34.75016941849951 37.0 34.5 40.0 26.0 41.0 70-71 34.47069656164874 36.0 34.0 39.0 26.0 41.0 72-73 34.14044728985435 36.0 34.0 39.0 26.0 41.0 74-75 33.76418662609906 35.0 34.0 38.5 25.0 40.0 76-77 32.06507640363654 34.5 31.5 36.5 22.5 39.0 78-79 32.99561982196861 35.0 33.5 37.0 24.5 39.0 80-81 32.89077099238423 35.0 34.0 36.5 24.5 39.0 82-83 32.62936081174789 35.0 34.0 36.0 24.0 38.0 84-85 32.382731378932746 35.0 33.0 36.0 24.0 37.0 86-87 32.17439957433368 35.0 33.0 35.0 23.0 37.0 88-89 32.005559436687555 35.0 33.0 35.0 23.0 36.0 90-91 31.84239353672281 35.0 33.0 35.0 21.5 36.0 92-93 31.696600589768998 35.0 33.0 35.0 20.0 36.0 94-95 31.591413131649368 35.0 33.0 35.0 20.0 36.0 96-97 31.49067524914755 35.0 33.0 35.0 20.0 35.0 98-99 31.395622235978976 35.0 33.0 35.0 19.0 35.0 100 31.337596014239576 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6097273350180537 1101 2 1.2042353881445962 1101 3 1.5546251013601982 1101 4 0.9664868473931776 1101 5 0.5850402248305571 1101 6 1.1261321185422304 1101 7 0.8131902490631759 1101 8 0.46809589078944214 1101 9 0.4857646689838333 1101 10-11 0.4240967818615715 1101 12-13 0.371047152799342 1101 14-15 0.49221426597019047 1101 16-17 0.533716696327879 1101 18-19 0.508691684678702 1101 20-21 0.5196326359109591 1101 22-23 0.5024388903057186 1101 24-25 0.4993466438336398 1101 26-27 0.5044731750629339 1101 28-29 0.48859592361065296 1101 30-31 0.4878566880954196 1101 32-33 0.545297393698597 1101 34-35 0.5536896206417126 1101 36-37 0.5355287867214429 1101 38-39 0.5631038992429467 1101 40-41 0.5709341667414378 1101 42-43 0.5788930219468114 1101 44-45 0.5296297198462909 1101 46-47 0.5533322358012427 1101 48-49 0.5372072343598546 1101 50-51 0.5490244843012277 1101 52-53 0.5601346463362873 1101 54-55 0.5489271025571938 1101 56-57 0.6006656148892162 1101 58-59 0.5750449088613578 1101 60-61 0.6277653856588401 1101 62-63 0.5443576518971369 1101 64-65 0.4761826835295082 1101 66-67 0.5435763209546565 1101 68-69 0.4932576062728984 1101 70-71 0.47885796544035486 1101 72-73 0.4722190766835226 1101 74-75 0.47890425002811554 1101 76-77 0.4237524829165551 1101 78-79 0.5434490247452501 1101 80-81 0.5137391618123957 1101 82-83 0.4700344492219912 1101 84-85 0.4639368448368586 1101 86-87 0.4861298351659329 1101 88-89 0.47881762794268923 1101 90-91 0.4659405065136788 1101 92-93 0.4587327079204151 1101 94-95 0.3971076278743375 1101 96-97 0.4367524901003428 1101 98-99 0.422967098135981 1101 100 0.458656981435805 1102 1 1.4982094021857257 1102 2 1.1209730169417753 1102 3 1.4861058320781346 1102 4 0.8869240308332849 1102 5 0.49237409840775115 1102 6 1.0582459355926517 1102 7 0.7599198434088308 1102 8 0.432342380787901 1102 9 0.4335266825115198 1102 10-11 0.36864336109050555 1102 12-13 0.3416598262796171 1102 14-15 0.4418078805599208 1102 16-17 0.4793761424902243 1102 18-19 0.4134847676326743 1102 20-21 0.4166491236087779 1102 22-23 0.41522667406996305 1102 24-25 0.42600589113251175 1102 26-27 0.4348727505175134 1102 28-29 0.41603321804877424 1102 30-31 0.4223691787367727 1102 32-33 0.4466602441811389 1102 34-35 0.4372478237295354 1102 36-37 0.44137424785280643 1102 38-39 0.47219159784499 1102 40-41 0.45379517191544494 1102 42-43 0.4952884944895928 1102 44-45 0.4490667358526039 1102 46-47 0.4357063246511217 1102 48-49 0.4338034743221968 1102 50-51 0.367729879817972 1102 52-53 0.4002439661891657 1102 54-55 0.43788460102365434 1102 56-57 0.4639366749462539 1102 58-59 0.40763817099511357 1102 60-61 0.4130846441278564 1102 62-63 0.40907024274641657 1102 64-65 0.39056205856901016 1102 66-67 0.3597628874343002 1102 68-69 0.3463679317991435 1102 70-71 0.34604950079219066 1102 72-73 0.26885984585273803 1102 74-75 0.3646130109679504 1102 76-77 0.17837331534354206 1102 78-79 0.3772819335803277 1102 80-81 0.40663852362542485 1102 82-83 0.43790400827845843 1102 84-85 0.3867543535481772 1102 86-87 0.3577806526569347 1102 88-89 0.3465212243204121 1102 90-91 0.35928298574437534 1102 92-93 0.34443963355033347 1102 94-95 0.2752540277552562 1102 96-97 0.3013674080499449 1102 98-99 0.330306233890834 1102 100 0.3441023371668521 1103 1 1.4607710346058234 1103 2 1.0878467348559084 1103 3 1.4188041750275993 1103 4 0.8978169687462483 1103 5 0.4961016032389409 1103 6 1.038120884173864 1103 7 0.7679388813570469 1103 8 0.4314718700775373 1103 9 0.44718135499024214 1103 10-11 0.3737891535799207 1103 12-13 0.32385123834955465 1103 14-15 0.3789199822714693 1103 16-17 0.4124172862652671 1103 18-19 0.3681354379545141 1103 20-21 0.3909912218066509 1103 22-23 0.38620466738965575 1103 24-25 0.38969230536837784 1103 26-27 0.3869666601696551 1103 28-29 0.35595989788902926 1103 30-31 0.3412241644949887 1103 32-33 0.38260883023725967 1103 34-35 0.38814303469265354 1103 36-37 0.3594573039620599 1103 38-39 0.39556812212921244 1103 40-41 0.39261003984336185 1103 42-43 0.4136068031864042 1103 44-45 0.3651924251608918 1103 46-47 0.38839813661083866 1103 48-49 0.37259025860235795 1103 50-51 0.34264451710511423 1103 52-53 0.34187060722033635 1103 54-55 0.4281806911405255 1103 56-57 0.4526556929280332 1103 58-59 0.3962844186021428 1103 60-61 0.35019154318504064 1103 62-63 0.32045307702000514 1103 64-65 0.3157602153627366 1103 66-67 0.34233615029138775 1103 68-69 0.3250375921077122 1103 70-71 0.30871295786264596 1103 72-73 0.28977218554798156 1103 74-75 0.3060001940995889 1103 76-77 0.15270444500138325 1103 78-79 0.347400024083548 1103 80-81 0.36827599588395543 1103 82-83 0.37525961514055695 1103 84-85 0.30585408668250835 1103 86-87 0.29646368438829285 1103 88-89 0.33747922252814533 1103 90-91 0.37096192963644015 1103 92-93 0.3749241582426883 1103 94-95 0.3152843924675146 1103 96-97 0.32522295410086954 1103 98-99 0.3727296147985264 1103 100 0.36312598420823505 1104 1 1.3996302125574864 1104 2 1.0298194493230852 1104 3 1.3959972749056568 1104 4 0.8548486436468181 1104 5 0.46178928967815835 1104 6 0.9823043130834819 1104 7 0.7133934208648753 1104 8 0.3975874862588853 1104 9 0.3855860709932841 1104 10-11 0.28233614808194574 1104 12-13 0.27604131991830627 1104 14-15 0.3199327062602322 1104 16-17 0.35331572135798694 1104 18-19 0.32980007546429846 1104 20-21 0.337713213734709 1104 22-23 0.3446890608003983 1104 24-25 0.3137962547785591 1104 26-27 0.33605786918343483 1104 28-29 0.3442440506791584 1104 30-31 0.3516319130942307 1104 32-33 0.36130896611023644 1104 34-35 0.3634833768005379 1104 36-37 0.3364617795594782 1104 38-39 0.35832859643123527 1104 40-41 0.3538127511382072 1104 42-43 0.3780583587917974 1104 44-45 0.3431497019587155 1104 46-47 0.39174506842218904 1104 48-49 0.3080305650896733 1104 50-51 0.30403997438055086 1104 52-53 0.3232316727897384 1104 54-55 0.30640752336510957 1104 56-57 0.34685656234694306 1104 58-59 0.3118274888916588 1104 60-61 0.3108922539252674 1104 62-63 0.265729599088381 1104 64-65 0.24813609470716358 1104 66-67 0.2860950045247179 1104 68-69 0.2391835446506505 1104 70-71 0.2259272363412279 1104 72-73 0.25632737472696476 1104 74-75 0.2553988327649037 1104 76-77 0.10888441857442643 1104 78-79 0.25888507088738066 1104 80-81 0.29693479976233306 1104 82-83 0.2820686782456363 1104 84-85 0.2451560708278251 1104 86-87 0.2725858380836641 1104 88-89 0.23888937847098646 1104 90-91 0.20757801764566963 1104 92-93 0.24017771228653473 1104 94-95 0.22494805725195022 1104 96-97 0.18445735857467938 1104 98-99 0.16598109870529854 1104 100 0.1556166973005766 1105 1 1.3773064229371066 1105 2 1.0200166430996518 1105 3 1.3583894729681738 1105 4 0.8498983928948789 1105 5 0.4363993386463463 1105 6 1.010345490445033 1105 7 0.7282645078227858 1105 8 0.360516759111178 1105 9 0.3503481891872724 1105 10-11 0.28282213882641116 1105 12-13 0.26825756846279347 1105 14-15 0.3267894781138523 1105 16-17 0.3156287934657769 1105 18-19 0.29781918646519756 1105 20-21 0.3199751074737023 1105 22-23 0.3148471583301884 1105 24-25 0.31101475368235043 1105 26-27 0.32794398351563103 1105 28-29 0.3477347178869721 1105 30-31 0.3175976391844628 1105 32-33 0.3525515139109885 1105 34-35 0.37083296146449385 1105 36-37 0.37227755864377343 1105 38-39 0.37829600014518405 1105 40-41 0.40664200787307436 1105 42-43 0.4025888590569906 1105 44-45 0.36725244240416544 1105 46-47 0.37830285393626895 1105 48-49 0.3346343796866691 1105 50-51 0.33833935306345353 1105 52-53 0.39570064052509935 1105 54-55 0.43005842304035014 1105 56-57 0.405577257820525 1105 58-59 0.3627541635024585 1105 60-61 0.3387128367645076 1105 62-63 0.30353871869807136 1105 64-65 0.3141033108182256 1105 66-67 0.33251464481429593 1105 68-69 0.2907187412566046 1105 70-71 0.30117451591587496 1105 72-73 0.2803795855671538 1105 74-75 0.29873800610849344 1105 76-77 0.17308161377977882 1105 78-79 0.31223546790315737 1105 80-81 0.30520364700775815 1105 82-83 0.33696781472793447 1105 84-85 0.32561163755290323 1105 86-87 0.33872186892307354 1105 88-89 0.31550526337503015 1105 90-91 0.317866506874811 1105 92-93 0.3317818319335011 1105 94-95 0.307466204742024 1105 96-97 0.3139251890779349 1105 98-99 0.3062004687099673 1105 100 0.3135135453016389 1106 1 1.3338943066673714 1106 2 0.9844118303219744 1106 3 1.3419486432716035 1106 4 0.8008196843666298 1106 5 0.40438626263907196 1106 6 0.9572748650501595 1106 7 0.6648819428530359 1106 8 0.3173535300622632 1106 9 0.30824708055020267 1106 10-11 0.22140071693231533 1106 12-13 0.19864476625088656 1106 14-15 0.25628595595230763 1106 16-17 0.24795867748283484 1106 18-19 0.19762938741769887 1106 20-21 0.23239936647681247 1106 22-23 0.2473873817806549 1106 24-25 0.23822378382453024 1106 26-27 0.2265101071803528 1106 28-29 0.2452023410470332 1106 30-31 0.2554110738120201 1106 32-33 0.286282341766686 1106 34-35 0.29729625657815006 1106 36-37 0.2874130052462931 1106 38-39 0.30923722146020083 1106 40-41 0.2875312026683048 1106 42-43 0.3591108870418154 1106 44-45 0.3182646004149703 1106 46-47 0.3167925565639038 1106 48-49 0.2666167556267922 1106 50-51 0.2770736972268111 1106 52-53 0.2656856249860411 1106 54-55 0.2683644792109732 1106 56-57 0.26987489416227106 1106 58-59 0.2087931464564079 1106 60-61 0.21909768097633986 1106 62-63 0.18734387239936723 1106 64-65 0.1337374709254462 1106 66-67 0.12388248174196548 1106 68-69 0.1700672517443209 1106 70-71 0.17049067411667096 1106 72-73 0.09535925501482012 1106 74-75 0.13286738459031966 1106 76-77 0.014452981534219589 1106 78-79 0.1563260032214302 1106 80-81 0.2045401687757007 1106 82-83 0.19631556393313332 1106 84-85 0.1802183538512665 1106 86-87 0.14998817673271247 1106 88-89 0.18309163828257624 1106 90-91 0.16191177029843473 1106 92-93 0.1639677982306189 1106 94-95 0.150370572770548 1106 96-97 0.18533006898386262 1106 98-99 0.2242583412357284 1106 100 0.24793689979163602 1107 1 1.2893396176207155 1107 2 0.904805912499242 1107 3 1.2840656025299957 1107 4 0.7786806783510727 1107 5 0.3717489144022892 1107 6 0.942359948564814 1107 7 0.626482515589629 1107 8 0.2654091635223139 1107 9 0.24445828468034847 1107 10-11 0.1599668160180343 1107 12-13 0.09785860412419112 1107 14-15 0.13897107091713679 1107 16-17 0.16690423438183188 1107 18-19 0.10861854316706143 1107 20-21 0.14028983126302563 1107 22-23 0.1744078611835036 1107 24-25 0.17181224504698633 1107 26-27 0.14584813237544125 1107 28-29 0.13061792774764314 1107 30-31 0.15227059994511905 1107 32-33 0.15651329965287886 1107 34-35 0.18006173010064686 1107 36-37 0.16873604378854168 1107 38-39 0.16057941249911067 1107 40-41 0.19871703410580466 1107 42-43 0.19526895686357193 1107 44-45 0.17939628626083248 1107 46-47 0.2075644843247133 1107 48-49 0.19730824625666088 1107 50-51 0.17761453776842018 1107 52-53 0.16959409873223308 1107 54-55 0.20757576484351148 1107 56-57 0.177968187515134 1107 58-59 0.15755397071822586 1107 60-61 0.15047654782993902 1107 62-63 0.12406316574817566 1107 64-65 0.11013988677205333 1107 66-67 0.11935111400834586 1107 68-69 0.10125405126715492 1107 70-71 0.0752893213629946 1107 72-73 0.07685399267833759 1107 74-75 0.12521395208244002 1107 76-77 -0.0036196819010356762 1107 78-79 0.1007054104140579 1107 80-81 0.18679334714943252 1107 82-83 0.20641837489120007 1107 84-85 0.1798792406336034 1107 86-87 0.18702412820435654 1107 88-89 0.17781867963431353 1107 90-91 0.17045703121260303 1107 92-93 0.1722300597619224 1107 94-95 0.14015907776283143 1107 96-97 0.16698308312381016 1107 98-99 0.1797182082360287 1107 100 0.17476404200138163 1108 1 1.25056841008141 1108 2 0.8820693483331681 1108 3 1.2342572586411613 1108 4 0.751171990908972 1108 5 0.3497484923360332 1108 6 0.8701782235795434 1108 7 0.6069674633771172 1108 8 0.27016030161509974 1108 9 0.21602727012926692 1108 10-11 0.14623089773290587 1108 12-13 0.12917234737502525 1108 14-15 0.14126284075700113 1108 16-17 0.16468654096263435 1108 18-19 0.11300945470245694 1108 20-21 0.13021243245558622 1108 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-1.414628094083163 2316 84-85 -1.0277439947707308 2316 86-87 -0.7698639654333661 2316 88-89 -0.669213788954039 2316 90-91 -0.7215080854309264 2316 92-93 -0.8152596096191864 2316 94-95 -0.8580824879456017 2316 96-97 -0.9601903568475443 2316 98-99 -1.0700231703055927 2316 100 -0.9818061002117169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 39.0 7 692.0 8 810.0 9 360.0 10 334.0 11 606.0 12 811.0 13 1220.0 14 2481.0 15 5605.0 16 11601.0 17 19379.0 18 26370.0 19 36445.0 20 51514.0 21 74784.0 22 114584.0 23 193222.0 24 341756.0 25 476217.0 26 480928.0 27 390092.0 28 325346.0 29 321392.0 30 361446.0 31 432938.0 32 531977.0 33 681259.0 34 930593.0 35 1451013.0 36 2612308.0 37 5265491.0 38 6839253.0 39 1998106.0 40 4012.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.727043151889298 34.374278655153425 27.26304729027004 21.63563090268723 2 20.80199618227305 33.9945439719279 27.428947569986068 17.774512275812988 3 26.787152856007378 25.778923336826914 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26.34812430266234 29.749276765915056 20.026228505656437 24-25 20.017415985495685 30.09287962742666 25.737584517634176 24.15211986944348 26-27 20.096642991308844 26.162336376315192 33.68514206257049 20.055878569805476 28-29 20.06719132421859 26.276011501012565 33.66306065971691 19.99373651505193 30-31 20.05212765611466 26.219715687647177 33.642414394289574 20.085742261948592 32-33 20.163222863118474 26.309905603168776 33.48165956497574 20.045211968737007 34-35 20.156342466097566 26.424445298535616 33.39183197656885 20.027380258797965 36-37 20.09651656557499 26.37811333865791 33.46689385652378 20.05847623924332 38-39 20.10612162222789 26.380378713750186 33.44195776566326 20.071541898358667 40-41 20.139404895032598 26.42807156443732 33.404772987538166 20.027750552991915 42-43 20.152342838692064 26.44954143877295 33.34005827387554 20.058057448659447 44-45 20.167960417841332 26.509692070968516 33.316836735523765 20.005510775666384 46-47 20.199828468334164 26.6173453347666 33.2267989116531 19.956027285246133 48-49 20.199467130277547 26.635884542488213 33.137300156909546 20.027348170324696 50-51 20.222338374152386 26.715542933585805 33.04838038859445 20.013738303667356 52-53 20.360014017580383 26.861920575388297 32.713163175482585 20.064902231548736 54-55 20.712528467837828 27.134980702314028 31.859068909356004 20.293421920492143 56-57 21.24637942371702 27.79548981784857 30.42288889793361 20.535241860500804 58-59 21.357197751722925 28.46969875772642 29.567472706551808 20.60563078399885 60-61 21.35462233841827 28.835166461012008 29.14494636747067 20.66526483309905 62-63 21.30257995703241 29.058667324130727 28.90782703068067 20.730925688156187 64-65 21.292910467060626 29.117369725266894 28.86376275000726 20.72595705766522 66-67 21.213545834481828 28.92678265970873 29.11551064613635 20.74416085967309 68-69 21.123856148215076 28.72214804071126 29.436566048172235 20.71742976290143 70-71 21.044459357429457 28.60490680037957 29.648621064805486 20.702012777385484 72-73 20.988179422349816 28.519486516571796 29.69708422942033 20.795249831658065 74-75 21.053421804883197 28.57654825872793 29.529674254092313 20.84035568229656 76-77 21.10052493845097 28.713727144351807 29.2819270600441 20.90382085715313 78-79 21.19592645404554 28.93502927309466 28.832210724565932 21.036833548293867 80-81 21.287764755625656 29.168082873681346 28.415830833383808 21.12832153730919 82-83 21.288091610806948 29.375708403146035 28.023401797139723 21.312798188907298 84-85 21.22440463989149 29.459051136366952 27.844342100685605 21.472202123055954 86-87 21.220068291611913 29.50354616734025 27.686266163075075 21.590119377972762 88-89 21.206355816660054 29.571673134387638 27.50362463832885 21.71834641062346 90-91 21.230086852867892 29.551779193963785 27.333012226968588 21.88512172619973 92-93 21.221743486756566 29.566164677249485 27.256734944948434 21.95535689104551 94-95 21.22207489827718 29.59346525619259 27.1254770605686 22.058982784961632 96-97 21.208587552536596 29.519225766429408 27.033580821502063 22.238605859531933 98-99 21.135714862242885 29.48868616997869 27.026738025910657 22.34886094186777 100 21.069358573903926 29.44898350697713 26.981678454247298 22.49997946487165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 33778.0 1 24443.0 2 12139.0 3 8570.0 4 7989.5 5 7887.0 6 7750.5 7 7699.0 8 7664.0 9 7828.0 10 8757.5 11 17941.5 12 139915.5 13 163370.5 14 71381.5 15 74081.0 16 83682.5 17 93013.0 18 101227.5 19 108998.5 20 118550.0 21 133442.0 22 153911.0 23 177849.5 24 202493.5 25 228599.5 26 259160.0 27 296935.5 28 342785.0 29 388202.0 30 425053.0 31 458081.5 32 494415.0 33 527201.5 34 552443.0 35 583282.0 36 619318.5 37 650203.5 38 682347.0 39 705851.0 40 714308.5 41 731203.0 42 758158.5 43 765061.0 44 762826.5 45 771128.0 46 777008.5 47 779901.0 48 777310.5 49 768998.0 50 762100.5 51 755412.5 52 739658.0 53 704544.5 54 656590.0 55 609857.5 56 562669.0 57 507621.0 58 446334.5 59 381285.0 60 313431.0 61 251375.5 62 195394.5 63 143896.0 64 103614.5 65 74372.5 66 52900.0 67 37950.5 68 27362.0 69 20064.0 70 14757.0 71 11042.5 72 8921.5 73 7025.5 74 5443.5 75 4597.5 76 3530.0 77 2127.5 78 1287.0 79 856.0 80 554.5 81 384.0 82 252.0 83 162.0 84 103.0 85 76.0 86 52.0 87 38.0 88 29.5 89 17.0 90 14.0 91 10.5 92 5.5 93 4.0 94 2.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3334669725024624 2 0.006683348214866435 3 2.402861723818536 4 8.046701219396269E-4 5 5.003130291852602E-5 6 0.008680431056364265 7 0.00814259454999011 8 3.0435709275436663E-4 9 4.169275243210502E-6 10-11 7.296231675618378E-5 12-13 2.084637621605251E-6 14-15 0.0 16-17 1.667710097284201E-5 18-19 0.0 20-21 3.6272694615931367E-4 22-23 4.5862027675315526E-5 24-25 5.107362172932866E-4 26-27 0.003477175552837559 28-29 0.006381075759733674 30-31 0.0056785528812527035 32-33 0.015524296368094303 34-35 0.009908282615489759 36-37 0.005109446810554471 38-39 0.005319995210336601 40-41 0.008499067583284607 42-43 0.019172412205903495 44-45 0.03470087784924101 46-47 0.03364605121270875 48-49 0.04046490087297953 50-51 0.040360668991899266 52-53 0.06288934776858722 54-55 0.033960831493571146 56-57 0.02241402370749966 58-59 0.016485314311654325 60-61 0.01771316587077982 62-63 0.0249322659543988 64-65 0.045361714646130265 66-67 0.04833023861929614 68-69 0.05109029883030149 70-71 0.061796997654866063 72-73 0.06943719453804931 74-75 0.06041696754936339 76-77 0.06028563537920225 78-79 0.05479261524627242 80-81 0.05211594054013128 82-83 0.04841153948653874 84-85 0.05079011101279034 86-87 0.058805542667862525 88-89 0.06544094421743203 90-91 0.061428016795841936 92-93 0.06595793434759015 94-95 0.07409010570947222 96-97 0.07355852311596288 98-99 0.04773403225951704 100 0.007050244436268958 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.3984984E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.306108977927884 #Duplication Level Percentage of deduplicated Percentage of total 1 59.57803724463393 21.630467128947256 2 19.756692620434222 14.345772706418167 3 8.217583381689611 8.950454333724963 4 3.7773918984459627 5.48569607669284 5 2.1173698764807445 3.8436730741045797 6 1.3884919861342988 3.024644481814283 7 0.9391503367653107 2.386782613128133 8 0.7080560896881368 2.056540924376238 9 0.5057390894223339 1.6525276645468743 >10 2.679931904605804 17.219418571673273 >50 0.2154330563230991 5.507541539018488 >100 0.11481994352685344 6.097051461561998 >500 4.8554557288116106E-4 0.11773749232386549 >1k 7.135309207882127E-4 0.5073499880954092 >5k 4.599793607081882E-5 0.12268699664859173 >10k+ 5.7497420088492064E-5 7.051654946925052 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1572485 6.556122780819866 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 36793 0.15340014402344399 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 34424 0.1435231309722783 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33832 0.14105492002829773 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00614551170849228 4.169275243210502E-6 0.0 0.0 0.0 2 0.006162188809465122 8.338550486421004E-6 0.0 8.338550486421004E-6 0.0 3 0.006203881561897227 8.338550486421004E-6 0.0 8.338550486421004E-6 0.0 4 0.00622889721335649 8.338550486421004E-6 0.0 8.338550486421004E-6 0.0 5 0.006249743589572542 1.667710097284201E-5 0.0 8.338550486421004E-6 4.169275243210502E-6 6 0.006278928516275016 1.667710097284201E-5 0.0 8.338550486421004E-6 4.169275243210502E-6 7 0.0062830977915182265 1.667710097284201E-5 0.0 8.338550486421004E-6 4.169275243210502E-6 8 0.006291436342004648 1.667710097284201E-5 0.0 8.338550486421004E-6 4.169275243210502E-6 9 0.006303944167734279 3.752347718889452E-5 0.0 8.338550486421004E-6 4.169275243210502E-6 10-11 0.006343552282544778 4.169275243210502E-5 0.0 1.2507825729631507E-5 4.169275243210502E-6 12-13 0.006376906484490463 4.586202767531552E-5 0.0 1.2507825729631507E-5 4.169275243210502E-6 14-15 0.006422768512165779 5.420057816173653E-5 0.0 1.2507825729631507E-5 4.169275243210502E-6 16-17 0.006481138365570726 5.420057816173653E-5 0.0 2.5015651459263013E-5 4.169275243210502E-6 18-19 0.006508238654651594 7.921622962099954E-5 0.0 2.9184926702473517E-5 4.169275243210502E-6 20-21 0.006512407929894804 1.0006260583705205E-4 0.0 3.335420194568402E-5 4.169275243210502E-6 22-23 0.006604131985245435 1.0423188108026255E-4 0.0 3.335420194568402E-5 6.253912864815753E-6 24-25 0.006879304151297328 1.1882434443149931E-4 0.0 3.543883956728927E-5 8.338550486421004E-6 26-27 0.0076881435484801655 1.3758608302594656E-4 0.0 3.752347718889452E-5 8.338550486421004E-6 28-29 0.010160523767703994 1.4383999589076232E-4 0.0 3.752347718889452E-5 8.338550486421004E-6 30-31 0.016166364755548723 1.542631839987886E-4 0.0 3.752347718889452E-5 8.338550486421004E-6 32-33 0.028740898889071595 1.709402849716306E-4 0.0 4.169275243210502E-5 8.338550486421004E-6 34-35 0.051957508080889274 1.8553274832286735E-4 0.0 4.169275243210502E-5 8.338550486421004E-6 36-37 0.0900813609048061 2.0637912453891984E-4 0.0 4.169275243210502E-5 1.667710097284201E-5 38-39 0.14760068216013822 2.1680231264694608E-4 0.0 4.169275243210502E-5 1.667710097284201E-5 40-41 0.22571622311693018 2.2931013837657762E-4 0.0 4.169275243210502E-5 1.667710097284201E-5 42-43 0.32572254373819887 2.459872393494196E-4 0.0 4.586202767531552E-5 1.667710097284201E-5 44-45 0.45255815054952714 2.6683361556547214E-4 0.0 4.586202767531552E-5 1.667710097284201E-5 46-47 0.6109926944291478 2.7100289080868263E-4 0.0 5.0031302918526027E-5 1.667710097284201E-5 48-49 0.8039488373225515 2.7934144129510366E-4 0.0 5.628521578334178E-5 2.084637621605251E-5 50-51 1.028189137003385 3.0018781751115615E-4 0.0 5.836985340494703E-5 2.084637621605251E-5 52-53 1.2782372504396917 3.1061100561918244E-4 0.0 5.836985340494703E-5 2.084637621605251E-5 54-55 1.5573347891330678 3.252034689704192E-4 0.0 5.836985340494703E-5 2.084637621605251E-5 56-57 1.8742372310942548 3.377112947000507E-4 0.0 6.045449102655228E-5 2.9184926702473514E-5 58-59 2.232767801721277 3.606423085377084E-4 0.0 6.253912864815753E-5 2.9184926702473514E-5 60-61 2.6256177615127863 3.6689622140252415E-4 0.0 6.253912864815753E-5 2.9184926702473514E-5 62-63 3.0434750342130727 3.835733223753662E-4 0.0 6.253912864815753E-5 3.126956432407877E-5 64-65 3.4843154366915567 3.919118728617872E-4 0.0 6.462376626976279E-5 3.335420194568402E-5 66-67 3.955697864964179 3.9816578572660294E-4 0.0 6.670840389136804E-5 3.335420194568402E-5 68-69 4.456769702243704 4.002504233482082E-4 0.0 6.670840389136804E-5 3.543883956728927E-5 70-71 4.983028131267464 4.0858897383462924E-4 0.0 6.670840389136804E-5 4.169275243210502E-5 72-73 5.5371539960168406 4.252660748074712E-4 0.0 6.670840389136804E-5 4.169275243210502E-5 74-75 6.109024296201323 4.356892629154975E-4 0.0 6.670840389136804E-5 4.169275243210502E-5 76-77 6.702727006196877 4.5653563913155E-4 0.0 7.504695437778904E-5 4.169275243210502E-5 78-79 7.3166381932962725 4.6278955199636573E-4 0.0 7.504695437778904E-5 4.169275243210502E-5 80-81 7.947968612361802 4.7529737772599725E-4 0.0 7.921622962099954E-5 4.169275243210502E-5 82-83 8.59577809182612 4.836359282124182E-4 0.0 7.921622962099954E-5 4.169275243210502E-5 84-85 9.257977824792377 4.940591163204444E-4 0.0 7.921622962099954E-5 4.169275243210502E-5 86-87 9.934957638495819 5.044823044284707E-4 0.0 7.921622962099954E-5 4.169275243210502E-5 88 10.452289649223864 5.086515796716813E-4 0.0 7.921622962099954E-5 4.169275243210502E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 205380 0.0 85.392296 8 AAGCAGT 199185 0.0 85.283455 1 AGTGGTA 205080 0.0 84.904236 5 GTGGTAT 205520 0.0 84.66992 6 TGGTATC 207295 0.0 84.556145 7 GCAGTGG 206555 0.0 84.371765 3 GTATCAA 207685 0.0 84.31087 9 AGCAGTG 212350 0.0 81.76242 2 CAGTGGT 213965 0.0 81.21061 4 CAACGCA 202405 0.0 44.471462 12-13 ATCAACG 203360 0.0 44.346912 10-11 ACGCAGA 205110 0.0 44.00182 14-15 AGAGTAC 206430 0.0 43.62063 18-19 GAGTACT 199795 0.0 43.498905 20-21 TCAACGC 202050 0.0 43.259098 12-13 AACGCAG 203535 0.0 43.026634 14-15 AGTACTT 208910 0.0 42.766033 20-21 CGCAGAG 206090 0.0 42.551376 16-17 TATCAAC 207565 0.0 42.13462 10-11 GTACTTT 210175 0.0 41.32755 22-23 >>END_MODULE