FastQCFastQC Report
Thu 26 May 2016
SRR1660703_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660703_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32943410
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23047926.996215631593694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA688560.20901297103123204No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT482900.14658470389070227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2851300.087.433648
GTGGTAT2883100.086.514126
TGGTATC2871550.086.517
AGTGGTA2890200.086.354915
GTATCAA2892000.086.069349
AAGCAGT2940400.085.424331
GCAGTGG2974350.084.4771963
CAGTGGT3000350.083.120224
AGCAGTG3079350.081.640312
CAACGCA2821950.045.43896512-13
ATCAACG2846800.045.1234210-11
ACGCAGA2854800.044.9470314-15
AGAGTAC2833700.044.58279418-19
TCAACGC2818850.044.3341212-13
AACGCAG2844100.044.02433814-15
GAGTACT2796100.043.7222420-21
CGCAGAG2871950.043.5235516-17
AGTACTT2896550.043.2924420-21
TATCAAC2880600.043.1466810-11
GTACTTT2916000.041.90809222-23
Produced by FastQC (version 0.11.5)