##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660703_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32943410 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74316444472506 34.0 31.0 34.0 30.0 34.0 2 31.86139221774552 34.0 31.0 34.0 30.0 34.0 3 31.922021278307255 34.0 31.0 34.0 30.0 34.0 4 35.3188494147995 37.0 35.0 37.0 33.0 37.0 5 35.28316315766947 37.0 35.0 37.0 33.0 37.0 6 35.36710540894218 37.0 35.0 37.0 33.0 37.0 7 35.296951469201275 37.0 35.0 37.0 33.0 37.0 8 35.322605795817736 37.0 35.0 37.0 33.0 37.0 9 36.98835108448093 39.0 37.0 39.0 33.0 39.0 10-11 36.89537091029739 39.0 37.0 39.0 33.0 39.0 12-13 36.952927444365955 39.0 37.0 39.0 33.0 39.0 14-15 38.32194488973667 40.0 38.0 41.0 33.0 41.0 16-17 38.294357778991305 40.0 38.0 41.0 33.0 41.0 18-19 38.24634254620271 40.0 38.0 41.0 33.0 41.0 20-21 38.108238294092814 40.0 38.0 41.0 33.0 41.0 22-23 37.90221649792781 40.0 37.5 41.0 32.5 41.0 24-25 37.90800827843869 40.0 38.0 41.0 32.0 41.0 26-27 37.923536846974855 40.0 38.0 41.0 32.0 41.0 28-29 37.85809887318891 40.0 38.0 41.0 32.0 41.0 30-31 37.77326717240261 40.0 38.0 41.0 32.0 41.0 32-33 37.65026521237479 40.0 38.0 41.0 31.5 41.0 34-35 37.49902327961799 40.0 37.0 41.0 31.0 41.0 36-37 37.29841983571221 39.5 37.0 41.0 31.0 41.0 38-39 36.80091728512622 39.0 35.0 41.0 30.0 41.0 40-41 36.735131882218624 39.0 35.0 41.0 30.0 41.0 42-43 36.728663471692826 39.0 35.0 41.0 30.0 41.0 44-45 36.727240455678384 39.0 35.0 41.0 30.0 41.0 46-47 36.81862229805597 40.0 35.0 41.0 30.0 41.0 48-49 36.71126891539157 40.0 35.0 41.0 30.0 41.0 50-51 35.34619984694966 38.0 34.0 40.0 27.5 40.5 52-53 35.48339699806425 38.5 34.5 39.5 28.0 40.5 54-55 35.75615622365748 39.0 35.0 41.0 25.5 41.0 56-57 35.28975529552041 39.0 35.0 41.0 22.5 41.0 58-59 35.09325009159647 39.0 35.0 41.0 22.0 41.0 60-61 34.88789985614726 39.0 34.5 41.0 22.0 41.0 62-63 34.67874543042144 38.0 34.0 41.0 21.5 41.0 64-65 34.44665042264902 37.5 34.0 40.0 21.0 41.0 66-67 34.2089835265991 37.0 34.0 40.0 21.0 41.0 68-69 33.966009620740536 36.5 34.0 40.0 21.0 41.0 70-71 33.69445690352031 36.0 33.0 39.0 21.0 41.0 72-73 33.36325400133137 35.5 33.0 39.0 20.5 41.0 74-75 32.98771297506846 35.0 33.0 38.5 20.0 40.0 76-77 32.594419642653875 35.0 33.0 37.0 20.0 39.5 78-79 32.21566671452652 35.0 32.0 37.0 19.0 39.0 80-81 31.89463731896607 35.0 32.0 36.0 18.0 39.0 82-83 31.580919552651046 35.0 32.0 36.0 17.5 37.5 84-85 31.295168912386423 35.0 31.5 35.5 17.0 37.0 86-87 31.087346983812544 35.0 31.0 35.0 16.0 37.0 88-89 30.88685486717981 35.0 31.0 35.0 15.0 36.0 90-91 30.71481791047132 35.0 31.0 35.0 15.0 36.0 92-93 30.560764489772005 35.0 31.0 35.0 14.0 36.0 94-95 30.380349787711715 35.0 31.0 35.0 11.5 35.5 96-97 30.248780302342716 35.0 30.0 35.0 10.5 35.0 98-99 30.150182995627958 34.0 30.0 35.0 10.0 35.0 100 30.071784189918407 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5149787032769844 1101 2 0.5523895201009772 1101 3 0.5604336752845072 1101 4 0.49816648111950457 1101 5 0.47872054857177204 1101 6 0.4499666790140324 1101 7 0.49265718670477554 1101 8 0.5116911812012717 1101 9 0.5954156108755555 1101 10-11 0.6082019523468887 1101 12-13 0.6004338824302948 1101 14-15 0.7154025104468076 1101 16-17 0.6707230843555791 1101 18-19 0.663003856687844 1101 20-21 0.7196826364078177 1101 22-23 0.7727487961033432 1101 24-25 0.7860809618106472 1101 26-27 0.702224842415049 1101 28-29 0.6665163475050733 1101 30-31 0.626237859659156 1101 32-33 0.6340816373794098 1101 34-35 0.6686362978291172 1101 36-37 0.6434063964685848 1101 38-39 0.6405188690878063 1101 40-41 0.6405910522176725 1101 42-43 0.7024530368096578 1101 44-45 0.726560737712397 1101 46-47 0.7098706171882299 1101 48-49 0.7404529047826713 1101 50-51 0.6678491283115662 1101 52-53 0.7520329776863832 1101 54-55 0.8053736659549102 1101 56-57 0.7713142267200368 1101 58-59 0.6409961029572955 1101 60-61 0.6698903826980711 1101 62-63 0.6453288654234584 1101 64-65 0.5896038048365355 1101 66-67 0.5423740110357897 1101 68-69 0.5685607413647205 1101 70-71 0.5603884809351456 1101 72-73 0.5401861002190245 1101 74-75 0.5086435962232585 1101 76-77 0.4916218358433966 1101 78-79 0.5097158970256288 1101 80-81 0.44332919881309607 1101 82-83 0.4881127909403915 1101 84-85 0.44185354512618247 1101 86-87 0.36515025519308963 1101 88-89 0.38399621710708587 1101 90-91 0.36794516339465133 1101 92-93 0.38724328213051606 1101 94-95 0.4311877674844631 1101 96-97 0.37263876193512857 1101 98-99 0.353721587554066 1101 100 0.3290176673024199 1102 1 0.4549136286389519 1102 2 0.4764112755377319 1102 3 0.4762600092710372 1102 4 0.43715711575588756 1102 5 0.47538417970324787 1102 6 0.3851143537011197 1102 7 0.4168043904164662 1102 8 0.43853890450128574 1102 9 0.5173276790210863 1102 10-11 0.5093857128022918 1102 12-13 0.5179757631259747 1102 14-15 0.5881111073435434 1102 16-17 0.5652910827629611 1102 18-19 0.5621360285156243 1102 20-21 0.5797055077416573 1102 22-23 0.6169954829578259 1102 24-25 0.5574542858244556 1102 26-27 0.5628205737541663 1102 28-29 0.5640267795965244 1102 30-31 0.5336790720543547 1102 32-33 0.5142952659942921 1102 34-35 0.5386175183917743 1102 36-37 0.5616688375246355 1102 38-39 0.5580403852135092 1102 40-41 0.49230942321123905 1102 42-43 0.5207973512367872 1102 44-45 0.5635243659231932 1102 46-47 0.5869310795300606 1102 48-49 0.6158956870117436 1102 50-51 0.5587067724133163 1102 52-53 0.6084539392884523 1102 54-55 0.6364542981981103 1102 56-57 0.6563638073966302 1102 58-59 0.5379033245244571 1102 60-61 0.5746874927272358 1102 62-63 0.5693718191028907 1102 64-65 0.5439111910912331 1102 66-67 0.5147821747121668 1102 68-69 0.4817921777975869 1102 70-71 0.46170584448801577 1102 72-73 0.448028601167735 1102 74-75 0.46754601722648204 1102 76-77 0.469936388665424 1102 78-79 0.4934014251222152 1102 80-81 0.4204767226282513 1102 82-83 0.4322430231165413 1102 84-85 0.4087298789991394 1102 86-87 0.3500769299608564 1102 88-89 0.3550139429324908 1102 90-91 0.3704665077918321 1102 92-93 0.333897018063805 1102 94-95 0.39038396212650994 1102 96-97 0.3288432778066799 1102 98-99 0.294905867437226 1102 100 0.28878770396516984 1103 1 0.39645612724227064 1103 2 0.41894644120113256 1103 3 0.41097099222439937 1103 4 0.36854789300677737 1103 5 0.3865217788144406 1103 6 0.380643398510216 1103 7 0.36039365321306605 1103 8 0.38885561329326634 1103 9 0.44257065738521817 1103 10-11 0.4500585182236847 1103 12-13 0.46299875527046197 1103 14-15 0.5374930930178721 1103 16-17 0.5070977287027247 1103 18-19 0.5183924290268394 1103 20-21 0.5462874911017863 1103 22-23 0.5793797421827449 1103 24-25 0.5985199707571596 1103 26-27 0.5602853136438171 1103 28-29 0.5404911943117767 1103 30-31 0.5151862737080677 1103 32-33 0.4733084071740805 1103 34-35 0.51731844170709 1103 36-37 0.4940124435208588 1103 38-39 0.5270551364417386 1103 40-41 0.5196217366105316 1103 42-43 0.5402730282804526 1103 44-45 0.5332028591275204 1103 46-47 0.5752883450269479 1103 48-49 0.617155082628436 1103 50-51 0.5561770703326019 1103 52-53 0.5615923618187608 1103 54-55 0.6362785897940952 1103 56-57 0.6806317150071521 1103 58-59 0.6403093208868142 1103 60-61 0.6396931887186383 1103 62-63 0.6202169264099524 1103 64-65 0.5741755599576877 1103 66-67 0.54744346363902 1103 68-69 0.5469524181655601 1103 70-71 0.5437469829069883 1103 72-73 0.5066819612982911 1103 74-75 0.5376168212824979 1103 76-77 0.5555673911767158 1103 78-79 0.4889045736668862 1103 80-81 0.45705728780075106 1103 82-83 0.46482774573476604 1103 84-85 0.46034503548882455 1103 86-87 0.4137307267230952 1103 88-89 0.39893360685564616 1103 90-91 0.42247427429762396 1103 92-93 0.3864433274777319 1103 94-95 0.4544268333255026 1103 96-97 0.4440997230194874 1103 98-99 0.40455862706322776 1103 100 0.39897321358852267 1104 1 0.3409689553716895 1104 2 0.39680741940435027 1104 3 0.3914404664937621 1104 4 0.30805174813985303 1104 5 0.32395692343194327 1104 6 0.2696999189766274 1104 7 0.2918939799326452 1104 8 0.3147275172819022 1104 9 0.3868330005006868 1104 10-11 0.364659663110821 1104 12-13 0.36336333538223897 1104 14-15 0.4319724429042253 1104 16-17 0.42156732719105605 1104 18-19 0.42283275287354627 1104 20-21 0.3609442510463765 1104 22-23 0.4140645903453972 1104 24-25 0.44228803461380295 1104 26-27 0.3622005338039287 1104 28-29 0.3677864348200046 1104 30-31 0.3273474627582331 1104 32-33 0.348283364714014 1104 34-35 0.3161605250575974 1104 36-37 0.29214722695533624 1104 38-39 0.3161267358266926 1104 40-41 0.3257157608882011 1104 42-43 0.32814711476798664 1104 44-45 0.3429097093364959 1104 46-47 0.350686384793093 1104 48-49 0.38626053365691604 1104 50-51 0.3481923606511046 1104 52-53 0.3670284937749031 1104 54-55 0.4624802317058112 1104 56-57 0.48766089596175277 1104 58-59 0.41889675233461077 1104 60-61 0.43681227762340313 1104 62-63 0.4145960936763018 1104 64-65 0.4002087243770873 1104 66-67 0.35446090583154444 1104 68-69 0.3529229356558261 1104 70-71 0.3510791875036645 1104 72-73 0.3487605554239863 1104 74-75 0.32202737928125913 1104 76-77 0.33437908711973563 1104 78-79 0.3251978343859534 1104 80-81 0.32283099258333436 1104 82-83 0.29792533527803045 1104 84-85 0.26570632238059133 1104 86-87 0.24599012752667448 1104 88-89 0.24103332935893107 1104 90-91 0.237694744607456 1104 92-93 0.2572039939159758 1104 94-95 0.325085939256585 1104 96-97 0.28636969591206096 1104 98-99 0.2633539503143929 1104 100 0.2195907877236145 1105 1 0.3201043978759195 1105 2 0.3725691661821564 1105 3 0.33174247926704936 1105 4 0.2925105032610844 1105 5 0.33239372330604056 1105 6 0.2720236281007402 1105 7 0.3004526792309008 1105 8 0.3060420417178378 1105 9 0.3182336012782869 1105 10-11 0.32658987631089786 1105 12-13 0.3314942992640084 1105 14-15 0.3713484686109183 1105 16-17 0.3453726372821535 1105 18-19 0.37381705062371395 1105 20-21 0.40854126579151995 1105 22-23 0.44167789145023306 1105 24-25 0.4594249081511137 1105 26-27 0.40941376207954505 1105 28-29 0.38634989262732944 1105 30-31 0.4296033362254974 1105 32-33 0.4179928755543827 1105 34-35 0.42591010347560854 1105 36-37 0.44459683555140117 1105 38-39 0.46169313746944596 1105 40-41 0.44384447528352666 1105 42-43 0.46683150596977896 1105 44-45 0.5160956075753091 1105 46-47 0.4952485256283552 1105 48-49 0.5340733661552051 1105 50-51 0.43259619099170976 1105 52-53 0.4779064870991405 1105 54-55 0.5810656852363252 1105 56-57 0.5906085027245496 1105 58-59 0.4974447377565028 1105 60-61 0.4953062048552255 1105 62-63 0.47441750434018815 1105 64-65 0.5088420605375106 1105 66-67 0.4301127520315049 1105 68-69 0.444638526491012 1105 70-71 0.44761449220339244 1105 72-73 0.4619599632059348 1105 74-75 0.4339601139122422 1105 76-77 0.4248554845961152 1105 78-79 0.4344475311300613 1105 80-81 0.37808442066470604 1105 82-83 0.40546397505001863 1105 84-85 0.39281239866285844 1105 86-87 0.32784276764273557 1105 88-89 0.3841728191748004 1105 90-91 0.38031172169655036 1105 92-93 0.3562451229719166 1105 94-95 0.40667195599745654 1105 96-97 0.42467673500565795 1105 98-99 0.3802570307433193 1105 100 0.3600779155486684 1106 1 0.22956146357127594 1106 2 0.2819648188496551 1106 3 0.2761370233511258 1106 4 0.25366375983614375 1106 5 0.26461986019924666 1106 6 0.2453398131110731 1106 7 0.24435040393282748 1106 8 0.2633671770854136 1106 9 0.26937685284474355 1106 10-11 0.24940593193341698 1106 12-13 0.2801968653220115 1106 14-15 0.33930617582123546 1106 16-17 0.30122181682394 1106 18-19 0.2811021810644192 1106 20-21 0.30849156656154975 1106 22-23 0.3012163084753894 1106 24-25 0.29688596052395866 1106 26-27 0.3073841343162158 1106 28-29 0.3186482134906683 1106 30-31 0.25827936781465866 1106 32-33 0.2916246951330308 1106 34-35 0.3179810663567082 1106 36-37 0.26171717958521157 1106 38-39 0.2822480105467875 1106 40-41 0.3289757250624845 1106 42-43 0.32003926640683744 1106 44-45 0.3420516508820839 1106 46-47 0.35678611508838287 1106 48-49 0.36740587585501316 1106 50-51 0.28220849531572867 1106 52-53 0.3412968469975013 1106 54-55 0.4664531077936189 1106 56-57 0.46737825681925926 1106 58-59 0.39511889770445663 1106 60-61 0.3882937671881308 1106 62-63 0.390693218110556 1106 64-65 0.3559718515113417 1106 66-67 0.38588881475855885 1106 68-69 0.3852366979372164 1106 70-71 0.3834527717518128 1106 72-73 0.38481300497918625 1106 74-75 0.38827206566467254 1106 76-77 0.401937121858694 1106 78-79 0.37898777188915034 1106 80-81 0.35673001967771967 1106 82-83 0.3893741212376014 1106 84-85 0.3635026443282605 1106 86-87 0.32510045479643424 1106 88-89 0.333631289688622 1106 90-91 0.29958116130886125 1106 92-93 0.30808256563587477 1106 94-95 0.32749041364475673 1106 96-97 0.32611196511147966 1106 98-99 0.33566456524814825 1106 100 0.32552172032564286 1107 1 0.20685953340763774 1107 2 0.2177681290641864 1107 3 0.2178422160477851 1107 4 0.24613167298620198 1107 5 0.24854483403603211 1107 6 0.2083822074351147 1107 7 0.2357515015292151 1107 8 0.24441434004219786 1107 9 0.2715742436436557 1107 10-11 0.23474999496693272 1107 12-13 0.2155400016405622 1107 14-15 0.2625643349154885 1107 16-17 0.24498799099584545 1107 18-19 0.22832108817122787 1107 20-21 0.24839966820160697 1107 22-23 0.3136203082140625 1107 24-25 0.3182051780668118 1107 26-27 0.28167116895551203 1107 28-29 0.2888374492545722 1107 30-31 0.23591796959561862 1107 32-33 0.20923812061374036 1107 34-35 0.2479585286770245 1107 36-37 0.23657286836792224 1107 38-39 0.27577393155859653 1107 40-41 0.288459292832016 1107 42-43 0.3006520248087554 1107 44-45 0.3143978621784598 1107 46-47 0.3119711732609076 1107 48-49 0.3517245426016089 1107 50-51 0.2752918790866019 1107 52-53 0.27715731716148895 1107 54-55 0.3588276177177434 1107 56-57 0.4334618035132891 1107 58-59 0.3757386472384283 1107 60-61 0.37486523477295464 1107 62-63 0.36823815148471084 1107 64-65 0.3242812172444971 1107 66-67 0.3516367743512916 1107 68-69 0.34129644032293527 1107 70-71 0.3295109495703983 1107 72-73 0.32993847077045046 1107 74-75 0.3287862162051951 1107 76-77 0.33840396849951304 1107 78-79 0.35103784138426875 1107 80-81 0.2704429238341035 1107 82-83 0.2904540398460753 1107 84-85 0.288358502571473 1107 86-87 0.2930652881826532 1107 88-89 0.30143223846039646 1107 90-91 0.28548744607706666 1107 92-93 0.2817206360340805 1107 94-95 0.32085676525811024 1107 96-97 0.3210380482126105 1107 98-99 0.30439118386432895 1107 100 0.32490359931419377 1108 1 0.09699447288653218 1108 2 0.11729250358967747 1108 3 0.15387764745435106 1108 4 0.09557887283049382 1108 5 0.1259969412441535 1108 6 0.06040422905842746 1108 7 0.10299798575009333 1108 8 0.13497055246331513 1108 9 0.14141405486173397 1108 10-11 0.10804509612342628 1108 12-13 0.09210024754793267 1108 14-15 0.15211951140965851 1108 16-17 0.1573795542129517 1108 18-19 0.12846528628826803 1108 20-21 0.11935105200971918 1108 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-1.338099565361862 2316 100 -1.1653130826262768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16968.0 3 4278.0 4 3006.0 5 2510.0 6 4553.0 7 23379.0 8 44863.0 9 51746.0 10 52892.0 11 52157.0 12 54307.0 13 59806.0 14 68839.0 15 80387.0 16 94455.0 17 110900.0 18 124372.0 19 139317.0 20 160522.0 21 193413.0 22 249403.0 23 358621.0 24 540673.0 25 703290.0 26 710078.0 27 588928.0 28 498820.0 29 473019.0 30 513960.0 31 614448.0 32 761354.0 33 979521.0 34 1347854.0 35 2067038.0 36 3587549.0 37 6626373.0 38 8378337.0 39 2596685.0 40 4789.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.665307382912646 35.33821184856698 27.15936659197677 21.8371141765436 2 20.533301690470577 34.791921818482166 26.660519711470965 18.014256779576286 3 26.91862861962099 25.447908114077407 26.262559060186735 21.370904206114876 4 18.736703959868223 27.450504346004735 24.734871345555078 29.07792034857196 5 20.981338446472453 34.14760268639485 25.776467903854446 19.09459096327825 6 29.1080057579637 26.64211371343231 25.48637020298454 18.763510325619453 7 20.348880330475012 25.765925691757275 33.80861508719348 20.07657889057423 8 28.153267832492862 26.159211971448435 25.63728349749075 20.050236698567954 9 27.566249794672537 25.064057304690877 27.03685146158501 20.332841439051574 10-11 19.912177560271505 30.3788835503884 29.80694832356387 19.90199056577622 12-13 19.860858249512667 26.359348770065633 29.72092483977335 24.05886814064835 14-15 19.834507079287324 34.51829147480405 25.6774413706099 19.969760075298723 16-17 24.065212800067364 26.106756499700534 25.75093645952372 24.077094240708384 18-19 19.93303711225492 30.263250142131394 25.746502959677247 24.05720978593644 20-21 24.061120376706764 30.32158995645603 25.69542767437744 19.921861992459768 22-23 24.02965400625903 26.254290911163185 29.76285536831468 19.95319971426311 24-25 20.001071328430086 30.153179760357563 25.624855876929946 24.220893034282405 26-27 20.03126425293736 26.091356490139777 33.88952474957061 19.98785450735225 28-29 20.01709889662528 26.197884831179998 33.87241164555975 19.91260462663497 30-31 20.002283849977918 26.111634197973327 33.877963892441244 20.00811805960751 32-33 20.07997961523188 26.229610540897834 33.70991703745179 19.9804928064185 34-35 20.1049931430789 26.33813277547512 33.615450044616594 19.941424036829385 36-37 20.061186753371448 26.280746122512156 33.65120569727233 20.006861426844058 38-39 20.09991210054181 26.271058434908333 33.65114136814437 19.97788809640548 40-41 20.11235675778955 26.319693423201073 33.62422269807905 19.943727120930323 42-43 20.117213365716513 26.334837272085544 33.580747660258474 19.96720170193947 44-45 20.133293158972478 26.393173007379723 33.54086643909802 19.93266739454978 46-47 20.112392720299937 26.48490708489505 33.500103917679816 19.902596277125195 48-49 20.2029377899742 26.478597916951973 33.37051808685521 19.94794620621861 50-51 20.204027860296584 26.550357703478838 33.30273450713372 19.94287992909086 52-53 20.326883628458322 26.708311947372778 32.99115948375418 19.97364494041472 54-55 20.757264142827637 27.020370847398066 32.02603310482632 20.19633190494798 56-57 21.32965174124364 27.698294935200572 30.588811480766914 20.38324184278887 58-59 21.43440692717464 28.345784589266266 29.790242508909557 20.429565974649535 60-61 21.50525659352259 28.67378991538993 29.402607789395624 20.41834570169186 62-63 21.48562337819271 28.856659125183153 29.24108926466532 20.416628231958818 64-65 21.507597306983236 28.9087634364675 29.251802136499517 20.331837120049748 66-67 21.533355730921606 28.64346293541568 29.55941873091626 20.263762602746453 68-69 21.574527564604512 28.372451466592807 29.920064679548624 20.132956289254054 70-71 21.618213171065374 28.20017911236193 30.1639122784062 20.017695438166495 72-73 21.720636635695 28.109859059607544 30.184619707950706 19.98488459674675 74-75 21.88043382980758 28.215680432927247 30.002227681712984 19.90165805555219 76-77 22.1196145281834 28.40164996077282 29.62628387887929 19.85245163216449 78-79 22.376743367651144 28.584068201253626 29.18988978956511 19.849298641530122 80-81 22.613103242540664 28.77280676556586 28.797356987361116 19.816733004532356 82-83 22.78819328028722 28.93747885524126 28.433222796873782 19.84110506759774 84-85 22.893235356730795 29.02293365458005 28.230244306853624 19.853586681835527 86-87 23.03494274515163 29.02658330887461 28.109715781263805 19.82875816470996 88-89 23.234550805400126 29.076403233285074 27.918597809025687 19.77044815228911 90-91 23.42742140616222 29.0517045868819 27.73201157419803 19.788862432757853 92-93 23.597534260796156 29.077389079088373 27.61351735054251 19.71155930957296 94-95 23.76449177859289 29.07996931796889 27.482255634159014 19.673283269279203 96-97 23.879803308182225 29.045995383793233 27.395776539799904 19.678424768224634 98-99 24.002949841569414 29.071996181642596 27.300083769713705 19.624970207074284 100 24.061849576153676 29.105472658740076 27.189962322238692 19.642715442867555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61353.0 1 42864.0 2 20468.0 3 15344.0 4 13883.0 5 13577.0 6 13501.5 7 13305.5 8 13102.5 9 13254.5 10 14165.0 11 18910.0 12 190395.5 13 234440.0 14 107950.5 15 112583.0 16 127598.0 17 140396.5 18 151072.5 19 161899.5 20 175651.5 21 197856.5 22 229594.5 23 261489.0 24 291096.0 25 324051.0 26 360641.0 27 407356.5 28 472233.5 29 534268.5 30 578939.5 31 628088.5 32 683567.0 33 725665.0 34 759908.0 35 811569.5 36 864742.5 37 895962.5 38 930085.5 39 958121.5 40 966755.0 41 990490.0 42 1032630.5 43 1037762.5 44 1020640.5 45 1024809.5 46 1030984.0 47 1032282.5 48 1026105.0 49 1014016.5 50 1002609.5 51 988819.0 52 967295.5 53 930467.0 54 880491.5 55 826501.0 56 769712.0 57 706076.5 58 632644.0 59 546584.0 60 452239.0 61 366182.5 62 289367.0 63 217769.5 64 160294.0 65 116094.5 66 82638.0 67 61452.5 68 46272.0 69 34675.0 70 26882.5 71 21548.0 72 18063.5 73 15200.0 74 13045.5 75 11917.5 76 9684.5 77 6423.5 78 4380.0 79 3530.0 80 2908.0 81 2375.0 82 1949.0 83 1666.5 84 1430.5 85 1187.0 86 1008.0 87 859.0 88 728.0 89 655.5 90 574.5 91 479.5 92 407.5 93 345.5 94 303.5 95 250.5 96 196.5 97 164.0 98 132.0 99 93.5 100 62.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4066792114113263 2 0.38600436323987103 3 0.17058343383395952 4 0.17651178187078992 5 0.2763951879905571 6 0.10277928119766594 7 0.13753888865785296 8 0.1430938691531933 9 0.1358147198483703 10-11 0.1324028083310137 12-13 0.13403135862377333 14-15 0.14015549695675097 16-17 0.14439154902300644 18-19 0.15575193946224752 20-21 0.1360621137884633 22-23 0.09930210624826029 24-25 0.09410228024360562 26-27 0.08846078775694441 28-29 0.09869804006324785 30-31 0.10341370246735235 32-33 0.10479941208272002 34-35 0.10530027097984088 36-37 0.1012205475996565 38-39 0.10042069111849683 40-41 0.10144062196354295 42-43 0.063654612561359 44-45 0.06652468581728485 46-47 0.0847908580198589 48-49 0.08919082754335389 50-51 0.09884070896121562 52-53 0.10429703543136548 54-55 0.10671937118834995 56-57 0.10734923919533527 58-59 0.10690150169639391 60-61 0.10473263089643726 62-63 0.10888520647983921 64-65 0.11604900646290109 66-67 0.124631906654472 68-69 0.1297603982101428 70-71 0.1283261204592967 72-73 0.11505639519406158 74-75 0.1123244375734024 76-77 0.11431117786531508 78-79 0.11313340057996425 80-81 0.10732647288182978 82-83 0.1102466320274677 84-85 0.11618408658970034 86-87 0.12975736270167537 88-89 0.13766638001348372 90-91 0.14465260275120273 92-93 0.1438603350412116 94-95 0.13363370701454405 96-97 0.12993190443855085 98-99 0.1253786417374522 100 0.11955350098851332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.294341E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.848525130028165 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02291168546253 25.80241020919509 2 14.746871434383163 10.86800925278325 3 5.450496299496541 6.025282495893714 4 2.6670241603094107 3.9310362717421437 5 1.5959146687465249 2.9403550888343446 6 1.0268471128610823 2.2702680985775077 7 0.7320864443239447 1.8883414018716689 8 0.5599213932654439 1.6505822024465673 9 0.41993755980185515 1.3926671752861228 >10 2.375143733254163 16.51773580941895 >50 0.21709228335429223 5.506064018214507 >100 0.18340268648641558 12.76024552362676 >500 0.0016742209549683597 0.3757070645759861 >1k 5.529860267741598E-4 0.4848567675130559 >5k 9.044293357869792E-5 0.2164369235175357 >10k+ 3.2888339305532646E-5 7.37000169650293 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2304792 6.996215631593694 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68856 0.20901297103123204 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 48290 0.14658470389070227 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00157542889458013 6.071016934798189E-6 0.0 3.0355084673990943E-6 0.0 2 0.001578464403047529 6.071016934798189E-6 0.0 6.071016934798189E-6 0.0 3 0.0015936419453845246 2.1248559271793662E-5 0.0 6.071016934798189E-6 9.106525402197283E-6 4 0.0015966774538519236 2.4284067739192754E-5 0.0 6.071016934798189E-6 1.8213050804394566E-5 5 0.0016027484707867219 3.0355084673990943E-5 0.0 6.071016934798189E-6 2.1248559271793662E-5 6 0.0016027484707867219 3.339059314139004E-5 0.0 9.106525402197283E-6 2.731957620659185E-5 7 0.0016088194877215201 3.339059314139004E-5 0.0 9.106525402197283E-6 3.642610160878913E-5 8 0.0016088194877215201 4.856813547838551E-5 0.0 9.106525402197283E-6 3.9461610076188224E-5 9 0.0016148905046563182 5.16036439457846E-5 3.0355084673990943E-6 9.106525402197283E-6 4.5532627010986416E-5 10-11 0.0016346213096944122 6.829894051647963E-5 3.0355084673990943E-6 1.0624279635896831E-5 4.705038124468596E-5 12-13 0.0016634586401347037 6.981669475017918E-5 3.0355084673990943E-6 1.5177542336995471E-5 5.767466088058279E-5 14-15 0.0016938137248086946 7.133444898387871E-5 3.0355084673990943E-6 3.0355084673990946E-5 6.526343204908053E-5 16-17 0.0017165800383141879 7.588771168497736E-5 3.0355084673990943E-6 3.7943855842488674E-5 7.285220321757828E-5 18-19 0.0017484528772218782 8.195872861977555E-5 3.0355084673990943E-6 4.0979364309887774E-5 8.499423708717465E-5 20-21 0.0017955032584665643 1.1686707599486512E-4 3.0355084673990943E-6 4.2497118543587323E-5 8.954749978827328E-5 22-23 0.001860766690515645 1.320446183318606E-4 3.0355084673990943E-6 4.856813547838551E-5 9.865402519047057E-5 24-25 0.0020899475798042766 1.4570440643515655E-4 3.0355084673990943E-6 4.856813547838551E-5 1.062427963589683E-4 26-27 0.0027714192307353733 1.6543521147325066E-4 3.0355084673990943E-6 4.856813547838551E-5 1.1686707599486514E-4 28-29 0.004987340411936712 1.669529657069502E-4 3.0355084673990943E-6 4.856813547838551E-5 1.1990258446226422E-4 30-31 0.010827658703212568 1.760594911091475E-4 3.0355084673990943E-6 5.008588971208506E-5 1.2749135563076196E-4 32-33 0.022978799098211142 1.9275478767984247E-4 4.5532627010986414E-6 5.16036439457846E-5 1.320446183318606E-4 34-35 0.04507426523240915 2.018613130820398E-4 6.071016934798189E-6 5.919241511428234E-5 1.3356237256556016E-4 36-37 0.08093120900356096 2.094500842505375E-4 6.071016934798189E-6 6.222792358168143E-5 1.381156352666588E-4 38-39 0.13288393642309645 2.185566096527348E-4 6.071016934798189E-6 6.526343204908053E-5 1.502576691362552E-4 40-41 0.20320149007039648 2.2462762658753297E-4 6.071016934798189E-6 6.981669475017918E-5 1.5481093183735382E-4 42-43 0.29344260354347046 2.2766313505493207E-4 6.071016934798189E-6 6.981669475017918E-5 1.6543521147325066E-4 44-45 0.40602657709083545 2.352519062234298E-4 6.071016934798189E-6 6.981669475017918E-5 1.8213050804394566E-4 46-47 0.545532475235563 2.41322923158228E-4 6.071016934798189E-6 6.981669475017918E-5 1.927547876798425E-4 48-49 0.7158093227143152 2.4284067739192754E-4 6.071016934798189E-6 7.436995745127781E-5 2.0489682154943887E-4 50-51 0.9137973269919538 2.4739394009302615E-4 6.071016934798189E-6 8.195872861977555E-5 2.124855927179366E-4 52-53 1.134635121257939 2.534649570278244E-4 6.071016934798189E-6 8.499423708717465E-5 2.215921181201339E-4 54-55 1.3811032312684084 2.747135162996181E-4 6.071016934798189E-6 8.499423708717465E-5 2.3069864352233117E-4 56-57 1.6635405988633236 2.807845332344162E-4 6.071016934798189E-6 8.802974555457373E-5 2.3676966045712937E-4 58-59 1.979150002989976 3.0355084673990945E-4 6.071016934798189E-6 9.106525402197283E-5 2.4284067739192754E-4 60-61 2.326984365006537 3.126573721421067E-4 6.071016934798189E-6 9.410076248937193E-5 2.4739394009302615E-4 62-63 2.6974074632832483 3.156928806095058E-4 6.071016934798189E-6 9.410076248937193E-5 2.565004654952235E-4 64-65 3.091156926377688 3.308704229465013E-4 6.071016934798189E-6 1.0017177942417012E-4 2.7167800783221895E-4 66-67 3.511498961400778 3.4301245681609766E-4 6.071016934798189E-6 1.0017177942417012E-4 2.792667790007167E-4 68-69 3.9631735148243608 3.460479652834968E-4 6.071016934798189E-6 1.0168953365786965E-4 2.944443213377121E-4 70-71 4.441681963099752 3.52118982218295E-4 6.071016934798189E-6 1.032072878915692E-4 3.0355084673990945E-4 72-73 4.939619183320731 3.657787703215909E-4 6.071016934798189E-6 1.0472504212526875E-4 3.2176389754430405E-4 74-75 5.45952134281181 3.7640304995748767E-4 6.071016934798189E-6 1.062427963589683E-4 3.293526687128017E-4 76-77 6.000329049117866 3.8399182112598543E-4 6.071016934798189E-6 1.062427963589683E-4 3.3694143988129946E-4 78-79 6.5641413563441064 3.8854508382708407E-4 6.071016934798189E-6 1.092783048263674E-4 3.5060122798459537E-4 80-81 7.148080602463437 4.0220487193038E-4 6.071016934798189E-6 1.092783048263674E-4 3.6426101608789133E-4 82-83 7.747986623121285 4.143469057999764E-4 6.071016934798189E-6 1.1838483022856469E-4 3.672965245552904E-4 84-85 8.361634390611052 4.2497118543587326E-4 6.071016934798189E-6 1.1838483022856469E-4 3.8095631265858636E-4 86-87 8.9899952676423 4.4166648200656824E-4 6.071016934798189E-6 1.2293809292966332E-4 3.915805922944832E-4 88 9.471077827098044 4.462197447076669E-4 6.071016934798189E-6 1.2445584716336286E-4 3.9461610076188227E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 285130 0.0 87.43364 8 GTGGTAT 288310 0.0 86.51412 6 TGGTATC 287155 0.0 86.51 7 AGTGGTA 289020 0.0 86.35491 5 GTATCAA 289200 0.0 86.06934 9 AAGCAGT 294040 0.0 85.42433 1 GCAGTGG 297435 0.0 84.477196 3 CAGTGGT 300035 0.0 83.12022 4 AGCAGTG 307935 0.0 81.64031 2 CAACGCA 282195 0.0 45.438965 12-13 ATCAACG 284680 0.0 45.12342 10-11 ACGCAGA 285480 0.0 44.94703 14-15 AGAGTAC 283370 0.0 44.582794 18-19 TCAACGC 281885 0.0 44.33412 12-13 AACGCAG 284410 0.0 44.024338 14-15 GAGTACT 279610 0.0 43.72224 20-21 CGCAGAG 287195 0.0 43.52355 16-17 AGTACTT 289655 0.0 43.29244 20-21 TATCAAC 288060 0.0 43.14668 10-11 GTACTTT 291600 0.0 41.908092 22-23 >>END_MODULE