FastQCFastQC Report
Thu 26 May 2016
SRR1660703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32943410
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19941766.053338133484056No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT529530.16073927987418424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA443960.13476443391865017No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT438430.1330857977361785No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2612100.086.175178
AAGCAGT2538450.085.6478351
GTGGTAT2621800.085.116176
TGGTATC2646000.085.061737
AGTGGTA2625850.085.015295
GCAGTGG2630500.084.730713
GTATCAA2660700.084.5528269
CAGTGGT2734800.081.451724
AGCAGTG2735800.081.2050862
CAACGCA2573450.045.08593812-13
ATCAACG2596850.044.7520110-11
ACGCAGA2611650.044.5470314-15
AGAGTAC2635100.044.03693418-19
TCAACGC2569250.043.69716612-13
AACGCAG2592950.043.38931714-15
GAGTACT2587400.043.12058320-21
AGTACTT2688050.042.87945620-21
CGCAGAG2626650.042.85685316-17
TATCAAC2643350.042.5059910-11
GTACTTT2707700.041.20340722-23
Produced by FastQC (version 0.11.5)