##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660703_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32943410 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.252053415235398 34.0 31.0 34.0 27.0 34.0 2 31.896148546856566 34.0 31.0 34.0 28.0 34.0 3 31.649097619220353 34.0 31.0 34.0 30.0 34.0 4 35.575118544194424 37.0 35.0 37.0 33.0 37.0 5 35.91061216795711 37.0 35.0 37.0 35.0 37.0 6 35.361490568219864 37.0 35.0 37.0 33.0 37.0 7 35.57191332044861 37.0 35.0 37.0 33.0 37.0 8 35.95066120963191 37.0 35.0 37.0 35.0 37.0 9 37.76095173511182 39.0 38.0 39.0 35.0 39.0 10-11 37.76339569886663 39.0 37.5 39.0 35.0 39.0 12-13 37.856756890073 39.0 38.0 39.0 35.0 39.0 14-15 39.31702445193136 41.0 39.0 41.0 36.0 41.0 16-17 39.28245635470038 41.0 39.0 41.0 36.0 41.0 18-19 39.27142510444426 41.0 39.0 41.0 36.0 41.0 20-21 39.200828815231944 40.5 39.0 41.0 36.0 41.0 22-23 39.06108746787294 40.0 38.5 41.0 35.5 41.0 24-25 39.08456934178945 40.0 39.0 41.0 36.0 41.0 26-27 39.0577013733551 40.0 39.0 41.0 35.5 41.0 28-29 38.98826816956715 40.0 39.0 41.0 35.0 41.0 30-31 38.899001120406176 40.0 38.0 41.0 35.0 41.0 32-33 38.78120036753937 40.0 38.0 41.0 35.0 41.0 34-35 38.69996014377382 40.0 38.0 41.0 35.0 41.0 36-37 38.5676035814143 40.0 38.0 41.0 35.0 41.0 38-39 38.13243053770087 40.0 37.0 41.0 34.0 41.0 40-41 38.003151722909074 40.0 37.0 41.0 33.5 41.0 42-43 37.84074643456764 40.0 36.0 41.0 33.0 41.0 44-45 37.72119076319058 40.0 36.0 41.0 33.0 41.0 46-47 37.57468000428614 40.0 36.0 41.0 33.0 41.0 48-49 37.62170013668894 40.0 36.0 41.0 33.0 41.0 50-51 37.617136097932786 40.0 36.0 41.0 33.0 41.0 52-53 37.35308554882448 40.0 35.5 41.0 32.5 41.0 54-55 36.7843896093331 40.0 35.0 41.0 30.5 41.0 56-57 36.239568748347544 39.0 35.0 41.0 28.5 41.0 58-59 36.09081696460688 39.0 35.0 41.0 28.0 41.0 60-61 35.955394204789364 39.0 35.0 41.0 28.0 41.0 62-63 35.73547484914282 39.0 35.0 41.0 28.0 41.0 64-65 35.48566318726568 38.0 35.0 40.5 28.0 41.0 66-67 35.22442057455497 37.0 35.0 40.0 27.5 41.0 68-69 34.97740658905681 37.0 35.0 40.0 27.0 41.0 70-71 34.69420116193193 36.5 34.0 39.0 27.0 41.0 72-73 34.35866331081087 36.0 34.0 39.0 26.0 41.0 74-75 33.978268157425106 35.5 34.0 38.5 26.0 40.0 76-77 32.28121587898764 34.5 31.5 36.5 24.0 39.0 78-79 33.212223582804576 35.0 33.5 37.0 26.0 39.0 80-81 33.10886168128921 35.0 34.0 36.5 26.0 39.0 82-83 32.84942313197085 35.0 34.0 36.0 25.0 38.0 84-85 32.59628654107149 35.0 34.0 36.0 25.0 37.0 86-87 32.38204566558228 35.0 33.5 35.5 24.5 37.0 88-89 32.20703227140117 35.0 33.0 35.0 24.0 36.5 90-91 32.04266231091438 35.0 33.0 35.0 24.0 36.0 92-93 31.894085584947035 35.0 33.0 35.0 23.5 36.0 94-95 31.783294913914496 35.0 33.0 35.0 23.0 36.0 96-97 31.67947490863878 35.0 33.0 35.0 23.0 35.5 98-99 31.583295445128478 35.0 33.0 35.0 20.0 35.0 100 31.521367065522362 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.557241958791078 1101 2 1.192733179695331 1101 3 1.5435319698455068 1101 4 0.9647390512985652 1101 5 0.5729013553969509 1101 6 1.1167158522220006 1101 7 0.8187494029775877 1101 8 0.4921480659578066 1101 9 0.5205144514270756 1101 10-11 0.43267627916941365 1101 12-13 0.40533086976673616 1101 14-15 0.5183178731142633 1101 16-17 0.5555739423877455 1101 18-19 0.5128039767750181 1101 20-21 0.5204674788412618 1101 22-23 0.5028669081515176 1101 24-25 0.48058151552039874 1101 26-27 0.4898281686721546 1101 28-29 0.48268915726448114 1101 30-31 0.47044925372959057 1101 32-33 0.5229507677146614 1101 34-35 0.5288479861245534 1101 36-37 0.5096229021312197 1101 38-39 0.5581341742824648 1101 40-41 0.5556832308205841 1101 42-43 0.5488037407888342 1101 44-45 0.5389682338359236 1101 46-47 0.549197160949582 1101 48-49 0.510439914323662 1101 50-51 0.4870423922524978 1101 52-53 0.4944899956266582 1101 54-55 0.4822966871119121 1101 56-57 0.5199804833243036 1101 58-59 0.49612805356326106 1101 60-61 0.49855092625894315 1101 62-63 0.46398036157798117 1101 64-65 0.46342715367193676 1101 66-67 0.4511176379349351 1101 68-69 0.4624574787780489 1101 70-71 0.43957568892330556 1101 72-73 0.42540119097865414 1101 74-75 0.4467993247996773 1101 76-77 0.3622433758243844 1101 78-79 0.4673916323379643 1101 80-81 0.46708693969643633 1101 82-83 0.4484584599412713 1101 84-85 0.4208923180983817 1101 86-87 0.4321795171056948 1101 88-89 0.4241003515910151 1101 90-91 0.44206676752525453 1101 92-93 0.40799784342476997 1101 94-95 0.362499099241095 1101 96-97 0.36863571154606944 1101 98-99 0.3634700358952223 1101 100 0.35160713889498396 1102 1 1.4902629532256135 1102 2 1.1398605336789274 1102 3 1.4854979783059683 1102 4 0.9070862146470731 1102 5 0.49224853484970765 1102 6 1.046325586862082 1102 7 0.7642777217167591 1102 8 0.43037708487410953 1102 9 0.44198539797658043 1102 10-11 0.3749388220543537 1102 12-13 0.35745312873481794 1102 14-15 0.46064688495146555 1102 16-17 0.481730956448267 1102 18-19 0.4220322404295018 1102 20-21 0.41958501843788554 1102 22-23 0.44603577810637773 1102 24-25 0.41208859792838837 1102 26-27 0.41056034830879895 1102 28-29 0.4334365637651132 1102 30-31 0.43360737851600817 1102 32-33 0.44379463130071883 1102 34-35 0.3840174345167 1102 36-37 0.4090389730736419 1102 38-39 0.4492238156680557 1102 40-41 0.4617915321369921 1102 42-43 0.48216143964985747 1102 44-45 0.43908114506967877 1102 46-47 0.4532633809975124 1102 48-49 0.439507291176362 1102 50-51 0.41149165330246973 1102 52-53 0.4009598108514467 1102 54-55 0.4578000660692325 1102 56-57 0.500024862688754 1102 58-59 0.5104031861893148 1102 60-61 0.494193786256659 1102 62-63 0.4457639130766111 1102 64-65 0.4231128793100254 1102 66-67 0.4296801056851649 1102 68-69 0.3815523366510263 1102 70-71 0.358810023862965 1102 72-73 0.33390795588756816 1102 74-75 0.3653750173643431 1102 76-77 0.20835253648147756 1102 78-79 0.4243437378227952 1102 80-81 0.45969629163107584 1102 82-83 0.4603319151061598 1102 84-85 0.4189887204701819 1102 86-87 0.39869677700606587 1102 88-89 0.3962232931504346 1102 90-91 0.4064830648346458 1102 92-93 0.3878621736513459 1102 94-95 0.3381943444118356 1102 96-97 0.35187247403322175 1102 98-99 0.3462305303000228 1102 100 0.3419951370410672 1103 1 1.4584559369153673 1103 2 1.0812947545465192 1103 3 1.409998114141441 1103 4 0.8844701717406664 1103 5 0.5088341304346145 1103 6 1.0409215156350413 1103 7 0.7713951445869824 1103 8 0.44388489682322074 1103 9 0.42845830104919713 1103 10-11 0.3600370350093698 1103 12-13 0.30853258808687656 1103 14-15 0.38516110376595236 1103 16-17 0.4082556045790042 1103 18-19 0.3736439033712031 1103 20-21 0.40168455572697326 1103 22-23 0.38589522226187967 1103 24-25 0.3750875707807779 1103 26-27 0.37964622649281665 1103 28-29 0.38746249583036274 1103 30-31 0.3871237717859728 1103 32-33 0.407630515344394 1103 34-35 0.4171029756586293 1103 36-37 0.37941791646847634 1103 38-39 0.4043205313175733 1103 40-41 0.44611609357433935 1103 42-43 0.46975811302077375 1103 44-45 0.46758140324114805 1103 46-47 0.45234092114809243 1103 48-49 0.4068426446853479 1103 50-51 0.39682780580293553 1103 52-53 0.39100515725267115 1103 54-55 0.42700661135130247 1103 56-57 0.46880758101424647 1103 58-59 0.45724717724227304 1103 60-61 0.4256251855835842 1103 62-63 0.3910311445261172 1103 64-65 0.34280384593939317 1103 66-67 0.3727337776587518 1103 68-69 0.3516499394095476 1103 70-71 0.3398208474828479 1103 72-73 0.3502285338395694 1103 74-75 0.3668691334185752 1103 76-77 0.21236228152714176 1103 78-79 0.41451407066273305 1103 80-81 0.4209382669785384 1103 82-83 0.43879753943459576 1103 84-85 0.3994322591470052 1103 86-87 0.3642307009146677 1103 88-89 0.3619611037832158 1103 90-91 0.36313139544286344 1103 92-93 0.38979815058928224 1103 94-95 0.32361174848167806 1103 96-97 0.3359685134228414 1103 98-99 0.3656196919642163 1103 100 0.37525155792513587 1104 1 1.3890555111505627 1104 2 1.0302171306113763 1104 3 1.3872594837203813 1104 4 0.8630188016073177 1104 5 0.46841648052269846 1104 6 1.0128726126771923 1104 7 0.7526561836588925 1104 8 0.4134953260673626 1104 9 0.408503221099906 1104 10-11 0.2965125799753565 1104 12-13 0.2774777232227521 1104 14-15 0.3261108783617388 1104 16-17 0.3381860554343703 1104 18-19 0.3132811811996703 1104 20-21 0.32950341542845507 1104 22-23 0.3259259286978917 1104 24-25 0.3061971967840975 1104 26-27 0.29842538780147976 1104 28-29 0.2811297171682554 1104 30-31 0.3014052503383553 1104 32-33 0.3206376515916034 1104 34-35 0.3491013911581078 1104 36-37 0.3240654719874456 1104 38-39 0.38584571112487254 1104 40-41 0.36423229597978235 1104 42-43 0.34757197352459457 1104 44-45 0.3320186968978973 1104 46-47 0.3595900819032849 1104 48-49 0.29164509307697273 1104 50-51 0.27865796919804353 1104 52-53 0.3203978475636333 1104 54-55 0.29075377872435126 1104 56-57 0.40141426282357173 1104 58-59 0.4243182208716618 1104 60-61 0.42173572382719726 1104 62-63 0.3419215686658177 1104 64-65 0.3138564095228489 1104 66-67 0.32326051278232626 1104 68-69 0.2637566264004789 1104 70-71 0.2706353268043884 1104 72-73 0.3001645306326495 1104 74-75 0.35564167668643876 1104 76-77 0.2344493955496958 1104 78-79 0.32320709915320833 1104 80-81 0.3665289351366994 1104 82-83 0.3658909729170077 1104 84-85 0.33841322469317703 1104 86-87 0.33117797054412534 1104 88-89 0.326791735427598 1104 90-91 0.33537202189496185 1104 92-93 0.33289128906908516 1104 94-95 0.3091478834445418 1104 96-97 0.2952018293587031 1104 98-99 0.3131638857777723 1104 100 0.3189007226675997 1105 1 1.3660905999873556 1105 2 1.0175854708566163 1105 3 1.348188766243542 1105 4 0.8290523020420864 1105 5 0.4342366580460464 1105 6 0.9846869171429233 1105 7 0.7087633419990453 1105 8 0.3590073232556392 1105 9 0.3712477306679105 1105 10-11 0.29032183697914604 1105 12-13 0.2664970278502423 1105 14-15 0.33270177745665563 1105 16-17 0.33219232732943027 1105 18-19 0.30605996869552143 1105 20-21 0.3119537797188059 1105 22-23 0.3253177340137512 1105 24-25 0.3188598350757914 1105 26-27 0.30601618643259343 1105 28-29 0.31796407092233636 1105 30-31 0.32484893829732897 1105 32-33 0.37030386204443033 1105 34-35 0.3800055195113572 1105 36-37 0.33450021077337055 1105 38-39 0.39109948335217126 1105 40-41 0.38573732372898206 1105 42-43 0.3689752512472708 1105 44-45 0.34697611616875434 1105 46-47 0.37926949986364633 1105 48-49 0.33493330148734657 1105 50-51 0.3426916621297096 1105 52-53 0.334574633860484 1105 54-55 0.395249883232502 1105 56-57 0.375345578094759 1105 58-59 0.3330475045682064 1105 60-61 0.3278926853995472 1105 62-63 0.30445694257518596 1105 64-65 0.2641179468018251 1105 66-67 0.27006815666265993 1105 68-69 0.25009955772567594 1105 70-71 0.2396574275742296 1105 72-73 0.26406600278901493 1105 74-75 0.2763499231926403 1105 76-77 0.1695878555045809 1105 78-79 0.2914749572340227 1105 80-81 0.3244492182484322 1105 82-83 0.32757515529225856 1105 84-85 0.29955263402386123 1105 86-87 0.28243840324008573 1105 88-89 0.2495496832451991 1105 90-91 0.24605492058432077 1105 92-93 0.26838908967618025 1105 94-95 0.2433067445813073 1105 96-97 0.2714992569842636 1105 98-99 0.27581719775832525 1105 100 0.2676201879156892 1106 1 1.3119291875110157 1106 2 0.9822418819844785 1106 3 1.3148038780020563 1106 4 0.8071795807425417 1106 5 0.4021353366703053 1106 6 0.9427954144507709 1106 7 0.6603685109082633 1106 8 0.32004097550169774 1106 9 0.303254662731689 1106 10-11 0.2295108260710208 1106 12-13 0.19347511754213542 1106 14-15 0.2522198952372676 1106 16-17 0.22493249927462955 1106 18-19 0.20985821703361296 1106 20-21 0.22684319995597235 1106 22-23 0.26423691714475694 1106 24-25 0.2468088157751538 1106 26-27 0.19353939082824212 1106 28-29 0.21335052732565174 1106 30-31 0.21510575960741818 1106 32-33 0.2551718721784155 1106 34-35 0.2528058878730306 1106 36-37 0.2410082998647809 1106 38-39 0.2870642214986816 1106 40-41 0.2869045589576089 1106 42-43 0.28324999515049143 1106 44-45 0.2859998416013312 1106 46-47 0.3045954216601814 1106 48-49 0.2797854581776491 1106 50-51 0.29217126953700756 1106 52-53 0.2797704277488222 1106 54-55 0.3450216621187394 1106 56-57 0.4118434552225736 1106 58-59 0.35414930943957046 1106 60-61 0.30518500447725216 1106 62-63 0.29763998272137115 1106 64-65 0.2563395335976111 1106 66-67 0.2303963827218638 1106 68-69 0.241487445376535 1106 70-71 0.2569688755177779 1106 72-73 0.18517313716085226 1106 74-75 0.263738205495919 1106 76-77 0.16205136303339884 1106 78-79 0.30895213753439066 1106 80-81 0.33987305287024583 1106 82-83 0.348404164549585 1106 84-85 0.3754442655486798 1106 86-87 0.3765195989076915 1106 88-89 0.3373498542078508 1106 90-91 0.3463563513422301 1106 92-93 0.36424557507485034 1106 94-95 0.3226698730985511 1106 96-97 0.33590509149129844 1106 98-99 0.33092051025218083 1106 100 0.3270428075278886 1107 1 1.280765559810039 1107 2 0.8970143042573042 1107 3 1.2688373662619945 1107 4 0.7778459449612072 1107 5 0.3816173851094362 1107 6 0.9496991729528474 1107 7 0.658733187273377 1107 8 0.2994073285047705 1107 9 0.2775201269633456 1107 10-11 0.19422639682668574 1107 12-13 0.14661406705747027 1107 14-15 0.18504206620725938 1107 16-17 0.18662090134248643 1107 18-19 0.152893544216532 1107 20-21 0.1624892522416772 1107 22-23 0.21026011094147634 1107 24-25 0.1884823852984283 1107 26-27 0.15891703597019813 1107 28-29 0.1521691922074595 1107 30-31 0.1717627091677585 1107 32-33 0.17556782352166778 1107 34-35 0.21363930994269253 1107 36-37 0.19112911527695786 1107 38-39 0.2206005288085393 1107 40-41 0.22066408332920417 1107 42-43 0.24800565501430327 1107 44-45 0.23411207074052953 1107 46-47 0.23478663536140232 1107 48-49 0.2294272137107427 1107 50-51 0.20132393057671294 1107 52-53 0.2011019428773153 1107 54-55 0.26943053176938747 1107 56-57 0.26756135961245064 1107 58-59 0.27121010223934405 1107 60-61 0.24372659647316652 1107 62-63 0.20759550224637024 1107 64-65 0.19073780211116542 1107 66-67 0.1914111161852361 1107 68-69 0.1592309668908598 1107 70-71 0.18488897246563596 1107 72-73 0.1858236697978981 1107 74-75 0.1817976056423234 1107 76-77 0.07246129763341713 1107 78-79 0.21287331318698222 1107 80-81 0.25642855982821544 1107 82-83 0.25109300924261646 1107 84-85 0.2623527298058548 1107 86-87 0.2712849001523807 1107 88-89 0.27883214749259366 1107 90-91 0.2541327293337119 1107 92-93 0.23836555569197415 1107 94-95 0.23513273590019068 1107 96-97 0.2797220626898351 1107 98-99 0.29278614673512493 1107 100 0.2702116851861547 1108 1 1.2302302654587116 1108 2 0.8767714289236324 1108 3 1.2257081181124612 1108 4 0.7464070992921634 1108 5 0.3425905004688161 1108 6 0.8781709403468057 1108 7 0.6125451238150603 1108 8 0.25332124398128997 1108 9 0.2024937515264824 1108 10-11 0.11007632831754677 1108 12-13 0.09929253544132877 1108 14-15 0.1342084611376677 1108 16-17 0.1544774685009429 1108 18-19 0.09807728789680681 1108 20-21 0.12451564809369131 1108 22-23 0.12396245223180813 1108 24-25 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-0.9478375437980375 2316 98-99 -1.08584869888902 2316 100 -1.0075466795228891 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 66.0 7 998.0 8 1291.0 9 514.0 10 397.0 11 780.0 12 1105.0 13 1591.0 14 3066.0 15 6739.0 16 13373.0 17 22464.0 18 30951.0 19 42816.0 20 61265.0 21 89486.0 22 138606.0 23 233260.0 24 415473.0 25 601387.0 26 636820.0 27 523262.0 28 430955.0 29 430009.0 30 489611.0 31 592443.0 32 731954.0 33 936892.0 34 1288539.0 35 2007833.0 36 3618330.0 37 7225816.0 38 9508616.0 39 2850767.0 40 5935.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.840412307007476 33.966617249226594 27.77067042953093 22.422300014235 2 20.84992703970383 33.63371599896591 27.914258993083557 17.602097968246696 3 25.8206436169723 26.11139742743333 26.658093974408715 21.40986498118566 4 18.916332069903728 28.00249193033717 25.376922221369576 27.704253778389525 5 21.243336828823793 33.52670844701569 26.150620527973466 19.079334196187048 6 28.874360962245248 26.89398371162199 25.734139407906525 18.49751591822624 7 20.394408442738182 26.20334000214991 33.3183934356089 20.083858119503013 8 27.056922366658576 26.360574547054426 26.370075718263504 20.212427368023494 9 26.590623939374336 25.17416154472659 27.856740630576514 20.378473885322553 10-11 19.977388781758957 30.147398041888962 29.86187635501419 20.013336821337894 12-13 19.949892252345414 26.65653434130837 29.64475579386389 23.748817612482327 14-15 19.930160538936313 33.8691364980128 26.065464989811314 20.135237973239565 16-17 23.746827304029036 26.35825370090505 26.10660537729347 23.78831361777245 18-19 20.03954053329634 30.088591314621045 26.131154910800063 23.740713241282553 20-21 23.694425484681584 30.121727166797342 26.11044608807978 20.073401260441294 22-23 23.637556536561526 26.50492953906131 29.753234749382006 20.104279174995156 24-25 20.11269898865295 29.945535262521144 26.003447474954765 23.938318273871136 26-27 20.151217806403196 26.34127271895791 33.36491128901519 20.142598185623708 28-29 20.12904421477245 26.445043470724432 33.34986672175435 20.076045592748766 30-31 20.11584312296339 26.369759816122702 33.331372789046775 20.183024271867133 32-33 20.256538720383343 26.48179687006063 33.14042633547569 20.121238074080335 34-35 20.230906440847114 26.58483095644961 33.066934622743695 20.11732797995958 36-37 20.186072064835166 26.526971712768294 33.115295958900546 20.171660263495998 38-39 20.215305710527907 26.523100586314293 33.12062837766992 20.14096532548788 40-41 20.234199566433144 26.568634980848927 33.09148770624555 20.105677746472374 42-43 20.228540078992438 26.58394745415005 33.032742787590855 20.154769679266657 44-45 20.25270525450781 26.635970993644186 32.98518975402291 20.126133997825093 46-47 20.285556214642902 26.747547160128924 32.882178512601975 20.084718112626202 48-49 20.32382001054646 26.746795560405502 32.80179596504576 20.127588464002276 50-51 20.33128228431386 26.836912966855675 32.70362769843585 20.128177050394612 52-53 20.42911561275237 26.978396572727036 32.420749962291026 20.171737852229562 54-55 20.78554434960658 27.23607959255034 31.62014942843152 20.35822662941156 56-57 21.289402055127283 27.844791213756505 30.28094483760481 20.584861893511402 58-59 21.3595832752126 28.466563056544448 29.50827635452125 20.665577313721702 60-61 21.343867595689755 28.794725033789714 29.16223580523839 20.69917156528214 62-63 21.273294704757827 28.99461029278092 28.986863323993095 20.74523167846816 64-65 21.247654600490602 29.08419447095364 28.944808993336384 20.723341935219374 66-67 21.171641034756515 28.905919329628695 29.186743485410748 20.735696150204046 68-69 21.109994314852226 28.69206872913767 29.48550361724911 20.712433338760995 70-71 21.052397384503017 28.584735349031753 29.663951786087605 20.69891548037762 72-73 21.013489554743074 28.531639329545055 29.67933809458017 20.775533021131697 74-75 21.093405789358556 28.62593321565243 29.493323150709656 20.78733784427936 76-77 21.143755815647307 28.788287901376098 29.241688187877408 20.82626809509919 78-79 21.242388816679593 29.013165383643972 28.810554319986224 20.93389147969021 80-81 21.33117632271094 29.22544190334042 28.430625957001364 21.012755816947273 82-83 21.34301128381047 29.42950599598252 28.07071809251257 21.156764627694447 84-85 21.342066779457134 29.523779963587476 27.851617930161666 21.282535326793724 86-87 21.376386973904825 29.570357759721954 27.694967245723827 21.35828802064939 88-89 21.39581935947915 29.664139229439822 27.510190456497675 21.429850954583358 90-91 21.46523500253244 29.684530333520144 27.30961342884544 21.540621235101977 92-93 21.506427453121624 29.731607374181618 27.190815466370793 21.571149706325965 94-95 21.58016188611367 29.738762287720615 27.071439009227984 21.60963681693773 96-97 21.634447150737536 29.714435914937265 26.948517735093162 21.702599199232036 98-99 21.6378158598288 29.73150486240724 26.882077352237477 21.74860192552648 100 21.613613056183155 29.736946709886457 26.79045879549433 21.858981438436054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 40281.0 1 30976.5 2 17494.5 3 12094.0 4 10744.0 5 10638.0 6 10716.5 7 10655.0 8 10454.5 9 10696.5 10 12150.5 11 24694.5 12 188130.5 13 218107.5 14 93722.5 15 98425.5 16 112297.5 17 124873.0 18 135898.5 19 146899.0 20 161533.0 21 185963.0 22 220127.5 23 255315.0 24 288665.5 25 324121.5 26 362809.5 27 411533.5 28 478458.5 29 542944.0 30 590178.5 31 641665.0 32 698626.5 33 740013.0 34 772805.0 35 824043.5 36 877409.0 37 907636.5 38 939173.0 39 966155.0 40 977115.0 41 1004513.5 42 1051504.5 43 1057617.5 44 1038012.0 45 1039873.0 46 1042791.5 47 1041221.5 48 1032801.5 49 1020873.5 50 1011796.5 51 999441.5 52 978320.0 53 942255.0 54 890081.5 55 832858.5 56 774187.5 57 707599.0 58 630632.5 59 540427.5 60 443761.5 61 358410.5 62 280314.5 63 207028.0 64 150030.5 65 107293.0 66 74875.0 67 54126.0 68 39857.0 69 28877.0 70 21428.0 71 16571.5 72 13689.5 73 11125.5 74 9129.5 75 8346.5 76 6411.5 77 3488.5 78 1843.0 79 1207.5 80 827.5 81 578.5 82 410.0 83 278.5 84 187.0 85 130.0 86 82.5 87 61.0 88 38.0 89 24.5 90 23.0 91 15.5 92 9.5 93 6.5 94 4.5 95 3.5 96 3.5 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3304090256594566 2 0.006696331679082402 3 2.415032323611915 4 8.317293200673518E-4 5 3.642610160878913E-5 6 0.008705838284500602 7 0.007688942947921907 8 3.126573721421067E-4 9 3.0355084673990943E-6 10-11 8.499423708717465E-5 12-13 4.5532627010986414E-6 14-15 0.0 16-17 4.7050381244685966E-5 18-19 0.0 20-21 3.2328165177800355E-4 22-23 4.7050381244685966E-5 24-25 5.114831767567474E-4 26-27 0.0034164647800576805 28-29 0.0060330730789557 30-31 0.0056991671475418 32-33 0.01491193534609805 34-35 0.009669612222899814 36-37 0.005029837530480299 38-39 0.005136080326839268 40-41 0.008195872861977555 42-43 0.018455891481786495 44-45 0.03330711665853656 46-47 0.03237369780481134 48-49 0.039233946941133295 50-51 0.039291621602013875 52-53 0.061341555109200904 54-55 0.032341824965903654 56-57 0.021565769906636865 58-59 0.016721098392667912 60-61 0.01782450572056748 62-63 0.0241247035446543 64-65 0.04427289099701579 66-67 0.047077700820892555 68-69 0.04973832399256786 70-71 0.06010610316296947 72-73 0.06760532683167893 74-75 0.05873101782723768 76-77 0.05859138443773732 78-79 0.05317603733189733 80-81 0.0505943373803744 82-83 0.0472643845916376 84-85 0.04941200683232246 86-87 0.05723147664434252 88-89 0.06393539709459342 90-91 0.059576406935408326 92-93 0.06433456645805641 94-95 0.07257748970127866 96-97 0.0717487958896787 98-99 0.04714903526987643 100 0.007066663712105092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.294341E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.78823022580922 #Duplication Level Percentage of deduplicated Percentage of total 1 67.62159241967655 24.2005711775122 2 15.90988161391037 11.387730121279871 3 6.055926709206554 6.501926978991341 4 2.795655403118304 4.002062367953014 5 1.7105943531017525 3.060957226588735 6 1.087216917356636 2.334574160625233 7 0.7909330138225923 1.9814264954313254 8 0.5899432885563438 1.6890420984820347 9 0.4319230085934924 1.3911984064231275 >10 2.576321496169228 17.350281052230912 >50 0.23567881277680425 5.816247687835144 >100 0.19224491933660767 12.778534810977572 >500 0.0013706837965353944 0.29299798824888046 >1k 6.152625670489896E-4 0.4976003588512776 >5k 5.104900558569004E-5 0.13762441199418304 >10k+ 5.104900553549957E-5 6.577224656575149 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1994176 6.053338133484056 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 52953 0.16073927987418424 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44396 0.13476443391865017 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 43843 0.1330857977361785 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0015268607591017445 0.0 0.0 0.0 0.0 2 0.0015359672845039417 0.0 0.0 6.071016934798189E-6 0.0 3 0.0015511448268409373 0.0 0.0 6.071016934798189E-6 0.0 4 0.0015572158437757355 1.2142033869596377E-5 0.0 9.106525402197283E-6 9.106525402197283E-6 5 0.0015572158437757355 2.4284067739192754E-5 0.0 9.106525402197283E-6 1.2142033869596377E-5 6 0.0015632868607105335 2.731957620659185E-5 0.0 9.106525402197283E-6 1.5177542336995471E-5 7 0.0015632868607105335 3.642610160878913E-5 0.0 1.2142033869596377E-5 2.4284067739192754E-5 8 0.0015693578776453318 3.642610160878913E-5 0.0 1.2142033869596377E-5 2.4284067739192754E-5 9 0.001581499911514928 3.9461610076188224E-5 0.0 1.5177542336995471E-5 2.731957620659185E-5 10-11 0.0015906064369171254 4.0979364309887774E-5 0.0 1.5177542336995471E-5 2.731957620659185E-5 12-13 0.0016042662250204216 4.2497118543587323E-5 0.0 1.5177542336995471E-5 3.642610160878913E-5 14-15 0.0016224792758248161 4.856813547838551E-5 0.0 3.0355084673990943E-5 4.856813547838551E-5 16-17 0.0016346213096944122 5.767466088058279E-5 0.0 3.339059314139004E-5 5.767466088058279E-5 18-19 0.0016649763943684033 6.678118628278008E-5 0.0 3.339059314139004E-5 6.678118628278008E-5 20-21 0.0016907782163412956 8.044097438607601E-5 0.0 3.339059314139004E-5 6.981669475017918E-5 22-23 0.0017545238941566767 8.651199132087418E-5 0.0 3.9461610076188224E-5 7.133444898387871E-5 24-25 0.001983704783445308 1.0320728789156922E-4 0.0 3.9461610076188224E-5 7.588771168497735E-5 26-27 0.0026773184682460016 1.259736013970624E-4 0.0 3.9461610076188224E-5 7.892322015237645E-5 28-29 0.00495850308149642 1.3356237256556016E-4 0.0 4.2497118543587323E-5 7.892322015237645E-5 30-31 0.010836765228614768 1.44186652201457E-4 0.0 4.705038124468596E-5 9.106525402197283E-5 32-33 0.022975763589743743 1.5329317760365426E-4 0.0 5.16036439457846E-5 1.0168953365786965E-4 34-35 0.04538692260455126 1.639174572395511E-4 0.0 6.0710169347981886E-5 1.0472504212526875E-4 36-37 0.08163696472223124 1.669529657069502E-4 0.0 6.222792358168143E-5 1.1686707599486514E-4 38-39 0.13420893586911617 1.7454173687544793E-4 0.0 6.374567781538098E-5 1.2142033869596377E-4 40-41 0.20527170684516266 1.8213050804394566E-4 0.0 6.678118628278008E-5 1.3052686409816106E-4 42-43 0.29615331260485783 1.9730805038094113E-4 0.0 6.829894051647963E-5 1.3356237256556016E-4 44-45 0.40953107161644775 2.0793233001683797E-4 0.0 7.588771168497735E-5 1.381156352666588E-4 46-47 0.5498762878524112 2.3980516892452844E-4 3.0355084673990943E-6 7.892322015237645E-5 1.4722216066885606E-4 48-49 0.720866479821002 2.519472027941248E-4 6.071016934798189E-6 7.892322015237645E-5 1.5481093183735382E-4 50-51 0.9196755891390721 2.565004654952235E-4 6.071016934798189E-6 8.0440974386076E-5 1.6239970300585153E-4 52-53 1.1420265843760558 2.5953597396262255E-4 6.071016934798189E-6 8.34764828534751E-5 1.669529657069502E-4 54-55 1.3904541151022314 2.747135162996181E-4 6.071016934798189E-6 8.802974555457373E-5 1.7757724534284703E-4 56-57 1.6749085780737332 2.8533779593551485E-4 6.071016934798189E-6 8.954749978827328E-5 1.9427254191354203E-4 58-59 1.9934609076595289 2.88373304402914E-4 6.071016934798189E-6 9.106525402197283E-5 2.018613130820398E-4 60-61 2.344478000304158 3.0962186367470765E-4 6.071016934798189E-6 9.106525402197283E-5 2.0337906731573933E-4 62-63 2.717699837387811 3.141751263758063E-4 6.071016934798189E-6 9.713627095677102E-5 2.1400334695163614E-4 64-65 3.114269287848465 3.263171602454027E-4 6.071016934798189E-6 1.0168953365786965E-4 2.3373415198973027E-4 66-67 3.5382493797697325 3.3390593141390033E-4 6.071016934798189E-6 1.0472504212526875E-4 2.3980516892452844E-4 68-69 3.994094721827522 3.3694143988129946E-4 6.071016934798189E-6 1.062427963589683E-4 2.504294485604253E-4 70-71 4.4770547432703545 3.672965245552904E-4 6.071016934798189E-6 1.0776055059266785E-4 2.5801821972892304E-4 72-73 4.979775924835954 3.809563126585863E-4 6.071016934798189E-6 1.1079605906006695E-4 2.7016025359851944E-4 74-75 5.504841484230078 3.8399182112598543E-4 6.071016934798189E-6 1.1231381329376649E-4 2.7774902476701715E-4 76-77 6.052020722809205 3.9765160922928134E-4 6.071016934798189E-6 1.1231381329376649E-4 2.914088128703131E-4 78-79 6.621600496123504 4.113113973325773E-4 6.071016934798189E-6 1.1231381329376649E-4 2.9596207557141174E-4 80-81 7.210918663247066 4.173824142673755E-4 6.071016934798189E-6 1.1838483022856467E-4 3.065863552073085E-4 82-83 7.817012567915707 4.310422023706714E-4 6.071016934798189E-6 1.2445584716336286E-4 3.1113961790840716E-4 84-85 8.43744469683011 4.431842362402678E-4 6.071016934798189E-6 1.2900910986446152E-4 3.1721063484320536E-4 86-87 9.072993657912159 4.507730074087655E-4 6.071016934798189E-6 1.3052686409816106E-4 3.247994060117031E-4 88 9.559511295278783 4.5532627010986415E-4 6.071016934798189E-6 1.3052686409816106E-4 3.3087042294650126E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 261210 0.0 86.17517 8 AAGCAGT 253845 0.0 85.647835 1 GTGGTAT 262180 0.0 85.11617 6 TGGTATC 264600 0.0 85.06173 7 AGTGGTA 262585 0.0 85.01529 5 GCAGTGG 263050 0.0 84.73071 3 GTATCAA 266070 0.0 84.552826 9 CAGTGGT 273480 0.0 81.45172 4 AGCAGTG 273580 0.0 81.205086 2 CAACGCA 257345 0.0 45.085938 12-13 ATCAACG 259685 0.0 44.75201 10-11 ACGCAGA 261165 0.0 44.54703 14-15 AGAGTAC 263510 0.0 44.036934 18-19 TCAACGC 256925 0.0 43.697166 12-13 AACGCAG 259295 0.0 43.389317 14-15 GAGTACT 258740 0.0 43.120583 20-21 AGTACTT 268805 0.0 42.879456 20-21 CGCAGAG 262665 0.0 42.856853 16-17 TATCAAC 264335 0.0 42.50599 10-11 GTACTTT 270770 0.0 41.203407 22-23 >>END_MODULE