FastQCFastQC Report
Thu 26 May 2016
SRR1660702_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660702_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26121242
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19583837.497281331416018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1087520.4163354866510559No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT481480.18432507918268204No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380630.14571665466749245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2483950.086.6266255
AAGCAGT2533650.086.6099551
GGTATCA2474350.086.496448
GTGGTAT2497650.085.897246
TGGTATC2490550.085.809027
GTATCAA2507450.084.643119
GCAGTGG2581650.084.596983
CAGTGGT2582600.083.746634
AGCAGTG2648850.083.024662
CAACGCA2458850.045.35120812-13
ATCAACG2460450.045.27912510-11
ACGCAGA2498650.044.93988814-15
AGAGTAC2504450.044.5307718-19
AGTACTT2532000.043.6986720-21
TCAACGC2457900.043.1180912-13
AACGCAG2474350.042.6514414-15
TATCAAC2493900.042.47761510-11
GAGTACT2464450.041.89420-21
CGCAGAG2514800.041.88873716-17
GCAGAGT2688550.041.56802416-17
Produced by FastQC (version 0.11.5)