##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660702_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26121242 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22884845980907 34.0 31.0 34.0 30.0 34.0 2 32.37275543789227 34.0 31.0 34.0 30.0 34.0 3 32.35039394374892 34.0 31.0 34.0 30.0 34.0 4 35.74344983289845 37.0 35.0 37.0 35.0 37.0 5 35.76550490975889 37.0 35.0 37.0 35.0 37.0 6 35.773593345982555 37.0 36.0 37.0 35.0 37.0 7 35.68977941401102 37.0 35.0 37.0 35.0 37.0 8 35.76560482843809 37.0 35.0 37.0 35.0 37.0 9 37.45414134595897 39.0 38.0 39.0 35.0 39.0 10-11 37.3436296214399 39.0 37.0 39.0 34.5 39.0 12-13 37.429627963325785 39.0 38.0 39.0 35.0 39.0 14-15 38.865076266281676 41.0 39.0 41.0 35.0 41.0 16-17 38.79676418142751 40.5 39.0 41.0 35.0 41.0 18-19 38.67593740374213 40.0 38.5 41.0 34.5 41.0 20-21 38.58990847372418 40.0 38.5 41.0 34.0 41.0 22-23 38.372623380618734 40.0 38.0 41.0 34.0 41.0 24-25 38.40116628068451 40.0 38.0 41.0 34.0 41.0 26-27 38.412122650982674 40.0 38.0 41.0 34.0 41.0 28-29 38.33852136510201 40.0 38.0 41.0 34.0 41.0 30-31 38.23377330220363 40.0 38.0 41.0 33.5 41.0 32-33 38.106449532529886 40.0 38.0 41.0 33.0 41.0 34-35 37.97885196270529 40.0 38.0 41.0 33.0 41.0 36-37 37.760654221571855 40.0 37.5 41.0 33.0 41.0 38-39 37.326282551955224 40.0 35.0 41.0 32.5 41.0 40-41 37.35528237899254 40.0 35.0 41.0 33.0 41.0 42-43 37.224896752612295 40.0 35.0 41.0 32.0 41.0 44-45 37.08334439074528 40.0 35.0 41.0 32.0 41.0 46-47 37.17634536673256 40.0 35.0 41.0 32.0 41.0 48-49 37.1145768451592 40.0 35.0 41.0 32.0 41.0 50-51 35.70817968762741 38.5 34.0 40.0 29.0 40.5 52-53 35.78733260462883 38.5 35.0 39.5 29.0 40.5 54-55 36.003432895725254 39.0 35.0 41.0 27.0 41.0 56-57 35.480873210393284 39.0 35.0 41.0 23.0 41.0 58-59 35.240990876314385 39.0 35.0 41.0 22.0 41.0 60-61 35.069226455618 39.0 35.0 41.0 22.0 41.0 62-63 34.83972559574312 38.5 35.0 41.0 22.0 41.0 64-65 34.56895263632564 37.5 34.0 40.5 22.0 41.0 66-67 34.295790280569356 37.0 34.0 40.0 22.0 41.0 68-69 34.04632249109748 36.5 34.0 40.0 22.0 41.0 70-71 33.73312013647743 36.0 34.0 39.0 21.0 41.0 72-73 33.41185897669031 35.5 33.0 39.0 20.5 41.0 74-75 33.02043871037985 35.0 33.0 38.5 20.0 40.0 76-77 32.61349358885768 35.0 33.0 37.0 19.5 39.5 78-79 32.21651700175666 35.0 33.0 37.0 18.5 39.0 80-81 31.820558111287358 35.0 32.0 36.0 17.5 39.0 82-83 31.527785661952827 35.0 32.0 36.0 16.0 37.5 84-85 31.23463428729767 35.0 32.0 35.0 15.0 37.0 86-87 30.977189656602086 35.0 31.5 35.0 14.0 37.0 88-89 30.806846818386354 35.0 31.0 35.0 12.5 36.0 90-91 30.610893961320826 35.0 31.0 35.0 11.5 36.0 92-93 30.450481221375306 35.0 31.0 35.0 10.0 36.0 94-95 30.292616369466657 35.0 31.0 35.0 10.0 35.5 96-97 30.14830577351567 35.0 30.5 35.0 10.0 35.0 98-99 30.015693434485236 34.0 30.0 35.0 10.0 35.0 100 29.90430294240986 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33942326519913735 1101 2 0.3130951461089424 1101 3 0.2957610454962065 1101 4 0.2538914067626763 1101 5 0.26739681017439665 1101 6 0.18631546913615438 1101 7 0.22763711858737423 1101 8 0.22340173032370814 1101 9 0.3263508281597751 1101 10-11 0.312621634598905 1101 12-13 0.31107518015191005 1101 14-15 0.40052773516730866 1101 16-17 0.34683659576463555 1101 18-19 0.41059346262591845 1101 20-21 0.394731786483014 1101 22-23 0.3760221809337665 1101 24-25 0.37349922043937767 1101 26-27 0.3663714217236489 1101 28-29 0.3843258984870559 1101 30-31 0.3596951403968802 1101 32-33 0.361055755971357 1101 34-35 0.38344920163462604 1101 36-37 0.3649260731146313 1101 38-39 0.4359962083855393 1101 40-41 0.376604707694824 1101 42-43 0.37967978137803726 1101 44-45 0.38373815451959814 1101 46-47 0.43863103369945833 1101 48-49 0.4199062900580941 1101 50-51 0.3263233262634344 1101 52-53 0.42339968185159904 1101 54-55 0.5028777343980337 1101 56-57 0.5313913831039656 1101 58-59 0.49960244076155647 1101 60-61 0.48953758322311103 1101 62-63 0.43202687260050965 1101 64-65 0.4376947279833203 1101 66-67 0.4408089603697789 1101 68-69 0.4497476411746817 1101 70-71 0.46277009915239375 1101 72-73 0.4268708105428729 1101 74-75 0.3859851286082332 1101 76-77 0.3574925755342875 1101 78-79 0.36249328977583417 1101 80-81 0.3794175830559716 1101 82-83 0.33689371010556357 1101 84-85 0.3299673098659994 1101 86-87 0.376228702607758 1101 88-89 0.31754772463167313 1101 90-91 0.31048674369947804 1101 92-93 0.2685603677035999 1101 94-95 0.2728483075408761 1101 96-97 0.2819697833525012 1101 98-99 0.23636070055759362 1101 100 0.17484258368699912 1102 1 0.3099144867484327 1102 2 0.31811673993582446 1102 3 0.3073152743455623 1102 4 0.27594584123916377 1102 5 0.2511377282050091 1102 6 0.19066591407012368 1102 7 0.21095415599420164 1102 8 0.20557313086610662 1102 9 0.31381531680282393 1102 10-11 0.2903638027885407 1102 12-13 0.2847109582629628 1102 14-15 0.3420594251326321 1102 16-17 0.3682040298133131 1102 18-19 0.38759259550580794 1102 20-21 0.3436522628632943 1102 22-23 0.3254207153098676 1102 24-25 0.3208064024473245 1102 26-27 0.3380949247446665 1102 28-29 0.33678619307652724 1102 30-31 0.3367373800465643 1102 32-33 0.2957355739335128 1102 34-35 0.32195828017113826 1102 36-37 0.2958036114215048 1102 38-39 0.3086277852951369 1102 40-41 0.2809043712864536 1102 42-43 0.25638744430704463 1102 44-45 0.2358364329700109 1102 46-47 0.2893246623809418 1102 48-49 0.29930890985870917 1102 50-51 0.23025703689229715 1102 52-53 0.3110851028787138 1102 54-55 0.4046196891542948 1102 56-57 0.3670649516190565 1102 58-59 0.38045124566320254 1102 60-61 0.32543232081762596 1102 62-63 0.30726391280881415 1102 64-65 0.30294865178095876 1102 66-67 0.3237422636631848 1102 68-69 0.2904852401721314 1102 70-71 0.31711253171349796 1102 72-73 0.21205336081587234 1102 74-75 0.2708527149570443 1102 76-77 0.2532625340784236 1102 78-79 0.2370429807833787 1102 80-81 0.28099992520007433 1102 82-83 0.2484670101736377 1102 84-85 0.18752205107458408 1102 86-87 0.2641073360146784 1102 88-89 0.20873189487275567 1102 90-91 0.2043466138424499 1102 92-93 0.20895288681995794 1102 94-95 0.1646443391912129 1102 96-97 0.19919965564171704 1102 98-99 0.15710473469038178 1102 100 0.09208446708980489 1103 1 0.25585826475075635 1103 2 0.22673150946673815 1103 3 0.24230764882852895 1103 4 0.2025334518290549 1103 5 0.20897417265765483 1103 6 0.1545840954027895 1103 7 0.1606193905565192 1103 8 0.17144812944484755 1103 9 0.23471657504801158 1103 10-11 0.23053660788782082 1103 12-13 0.25263090882899064 1103 14-15 0.27235045565551275 1103 16-17 0.28355788694923945 1103 18-19 0.3314015667967922 1103 20-21 0.30254706638210394 1103 22-23 0.2825360119607794 1103 24-25 0.2748407849303547 1103 26-27 0.2815249227713821 1103 28-29 0.2882121853949329 1103 30-31 0.24824870273631205 1103 32-33 0.211760901610468 1103 34-35 0.26075578364814334 1103 36-37 0.2011084270409782 1103 38-39 0.17699587470093547 1103 40-41 0.181366277410703 1103 42-43 0.14811492130424853 1103 44-45 0.171011097803067 1103 46-47 0.1820307520983917 1103 48-49 0.17667535020358116 1103 50-51 0.15482157286029263 1103 52-53 0.23168550699416102 1103 54-55 0.2661099401059843 1103 56-57 0.25131088175000826 1103 58-59 0.225155404754652 1103 60-61 0.19342340742493036 1103 62-63 0.1506724702381561 1103 64-65 0.16261798083015577 1103 66-67 0.18016138547578464 1103 68-69 0.13487602655318653 1103 70-71 0.19006811037896654 1103 72-73 0.1471968470170708 1103 74-75 0.18305203264003467 1103 76-77 0.13142992052297586 1103 78-79 0.14757198174994812 1103 80-81 0.1983984430209098 1103 82-83 0.16235685205218076 1103 84-85 0.1707918484519233 1103 86-87 0.12692438767003722 1103 88-89 0.09899282580717994 1103 90-91 0.11740501007656334 1103 92-93 0.10060477548803703 1103 94-95 0.08118950802621683 1103 96-97 0.08521602330275968 1103 98-99 0.026113702320980536 1103 100 0.04740427791677604 1104 1 0.2455621571247093 1104 2 0.21175375986829437 1104 3 0.20538681462170416 1104 4 0.22939268464683948 1104 5 0.18661644320251014 1104 6 0.13924921671998902 1104 7 0.15182149694462055 1104 8 0.1595123628292967 1104 9 0.24348743751504998 1104 10-11 0.24310593056089402 1104 12-13 0.23818826850424557 1104 14-15 0.2643456780147204 1104 16-17 0.2682584133806216 1104 18-19 0.29894696069462867 1104 20-21 0.2695939792253128 1104 22-23 0.28860008009796445 1104 24-25 0.2856581791653312 1104 26-27 0.28828503060508126 1104 28-29 0.23521185957522306 1104 30-31 0.2668970814074001 1104 32-33 0.24583994896076433 1104 34-35 0.24821961641853107 1104 36-37 0.22477925349502925 1104 38-39 0.2493183467971889 1104 40-41 0.22902510230317574 1104 42-43 0.22610505622089505 1104 44-45 0.20098576131655221 1104 46-47 0.21190709166499744 1104 48-49 0.2654784659996352 1104 50-51 0.2403433649256712 1104 52-53 0.25281999993249116 1104 54-55 0.2962161637312306 1104 56-57 0.31436395066095457 1104 58-59 0.30933379243972325 1104 60-61 0.2856420831537747 1104 62-63 0.24631015937247014 1104 64-65 0.26083090242774887 1104 66-67 0.289480583946677 1104 68-69 0.23441719269852967 1104 70-71 0.24807757723198876 1104 72-73 0.18700428425859883 1104 74-75 0.20940981174937434 1104 76-77 0.1851587623698876 1104 78-79 0.18729684975885164 1104 80-81 0.2039562545255187 1104 82-83 0.18621102491480102 1104 84-85 0.20071716310434695 1104 86-87 0.1942667608939317 1104 88-89 0.18422186762560244 1104 90-91 0.13922766184465019 1104 92-93 0.11994273438769554 1104 94-95 0.12225221239265593 1104 96-97 0.12953063195404368 1104 98-99 0.10993478719516858 1104 100 0.10779821498330122 1105 1 0.1890524977344299 1105 2 0.16391773831270484 1105 3 0.17072868067374003 1105 4 0.1803575143877083 1105 5 0.16489007974439573 1105 6 0.06861670835441913 1105 7 0.1017417650216288 1105 8 0.09191588719920674 1105 9 0.12283407677887226 1105 10-11 0.11960974717214157 1105 12-13 0.13598234030064305 1105 14-15 0.15801418692647928 1105 16-17 0.1653906406035972 1105 18-19 0.2431049752772978 1105 20-21 0.1878868163764693 1105 22-23 0.17362853646557141 1105 24-25 0.14708001266510706 1105 26-27 0.1904464406134352 1105 28-29 0.15845222375606483 1105 30-31 0.14218869204896833 1105 32-33 0.13599231749191887 1105 34-35 0.14420993967417672 1105 36-37 0.10358916518970318 1105 38-39 0.1396786870080149 1105 40-41 0.13954377028132114 1105 42-43 0.17249026126767575 1105 44-45 0.12151955442747209 1105 46-47 0.1551532713751982 1105 48-49 0.14798627708424306 1105 50-51 0.09269830020360104 1105 52-53 0.14760660615885968 1105 54-55 0.22174001362684947 1105 56-57 0.2654672728030363 1105 58-59 0.2753433807159027 1105 60-61 0.2361222148427231 1105 62-63 0.2410744164595613 1105 64-65 0.22958002879475004 1105 66-67 0.28713290205124054 1105 68-69 0.24279350662212096 1105 70-71 0.2618861873872689 1105 72-73 0.22822243500544204 1105 74-75 0.20801075831301574 1105 76-77 0.16345105904215274 1105 78-79 0.2084609640328594 1105 80-81 0.271795835253382 1105 82-83 0.20941342455866518 1105 84-85 0.19975698134458142 1105 86-87 0.19321294154168456 1105 88-89 0.1596605513678604 1105 90-91 0.2025433202117668 1105 92-93 0.20910584073169147 1105 94-95 0.1538481311940494 1105 96-97 0.16425399089645154 1105 98-99 0.10922599501933661 1105 100 0.1032525264232298 1106 1 0.09686118506957087 1106 2 0.08340238825479673 1106 3 0.1240448108169403 1106 4 0.10679185534647928 1106 5 0.09210448723052167 1106 6 0.03042659722418506 1106 7 0.0599728695849322 1106 8 0.057744814180750836 1106 9 0.09896465277680022 1106 10-11 0.06471251398590283 1106 12-13 0.06806982882538648 1106 14-15 0.08867419105921215 1106 16-17 0.08522940937500323 1106 18-19 0.14691498721372653 1106 20-21 0.08916633120754369 1106 22-23 0.1242466813251113 1106 24-25 0.08052560257618069 1106 26-27 0.12143815482610876 1106 28-29 0.06166342829806837 1106 30-31 0.07329521880851786 1106 32-33 0.06622957330922219 1106 34-35 2.7273338803013303E-4 1106 36-37 0.0211018905513356 1106 38-39 0.004314572756953794 1106 40-41 0.03108849887399856 1106 42-43 0.04352115997435391 1106 44-45 0.02372589141142356 1106 46-47 0.008550967859108027 1106 48-49 0.00946139350901376 1106 50-51 -0.0802782209446562 1106 52-53 -0.008245992809186475 1106 54-55 0.03197965982351292 1106 56-57 0.11043120686511543 1106 58-59 0.04657122806322178 1106 60-61 -0.03567573849232275 1106 62-63 -0.012573761617154844 1106 64-65 -0.026072307584335874 1106 66-67 -0.01851073840443007 1106 68-69 -0.02465628278576304 1106 70-71 0.04462260373762916 1106 72-73 -0.02677167588542062 1106 74-75 -0.08297524566683734 1106 76-77 -0.0727678491948538 1106 78-79 2.502444806538051E-4 1106 80-81 0.03574042230986052 1106 82-83 0.04723138652024517 1106 84-85 0.049918534249140833 1106 86-87 0.019882127024363427 1106 88-89 0.008870307559128321 1106 90-91 0.04147082436444549 1106 92-93 0.037929331749236184 1106 94-95 0.06931585749254054 1106 96-97 0.015820559469634787 1106 98-99 0.011047988581278645 1106 100 -0.012971640949793084 1107 1 0.006985097769224069 1107 2 0.024286828275265293 1107 3 0.0033387325001896784 1107 4 0.07717378836991173 1107 5 0.056250048663955 1107 6 -0.0037392870283099455 1107 7 -0.02751041085065964 1107 8 -0.03511007268798494 1107 9 -0.002852686670294702 1107 10-11 -0.01159010386577819 1107 12-13 0.012653100914157278 1107 14-15 -0.038999901878980836 1107 16-17 -0.027936366831717407 1107 18-19 0.0038429754254067916 1107 20-21 -0.010996507494802188 1107 22-23 -0.014843396929073549 1107 24-25 -0.03303576957639365 1107 26-27 -0.012110667578724588 1107 28-29 0.036222718853899494 1107 30-31 -0.011325846158918296 1107 32-33 -0.06220903921710175 1107 34-35 -0.03119872952138536 1107 36-37 -0.03618999805203771 1107 38-39 0.0020222907520661693 1107 40-41 0.021683489540095024 1107 42-43 -0.042542663157433935 1107 44-45 0.01160477577260366 1107 46-47 0.03603658715847757 1107 48-49 0.0012271327216382133 1107 50-51 -0.06527306523027931 1107 52-53 0.048422198787086757 1107 54-55 0.0859773094688947 1107 56-57 0.10596546128466144 1107 58-59 0.08670137454105742 1107 60-61 0.0901711919305086 1107 62-63 0.0859467255048898 1107 64-65 0.07853810282521323 1107 66-67 0.10090517445546254 1107 68-69 0.09707978134458983 1107 70-71 0.10825485998070405 1107 72-73 0.11186908277783658 1107 74-75 0.10986259646281127 1107 76-77 0.07982154671119446 1107 78-79 0.08804198202742697 1107 80-81 0.15272522552052337 1107 82-83 0.1197001749377371 1107 84-85 0.11468684708968624 1107 86-87 0.11361464639161056 1107 88-89 0.07435919153249415 1107 90-91 0.08508600554539214 1107 92-93 0.06367702205143644 1107 94-95 0.0923406188063467 1107 96-97 0.07600522597127934 1107 98-99 0.08638196492260519 1107 100 0.051277489616900596 1108 1 -0.03443684598677521 1108 2 -0.05491369806167512 1108 3 -0.0038871131001627646 1108 4 0.04397447706902824 1108 5 0.021679441983465608 1108 6 -0.0017788628551826946 1108 7 -0.03671125540122944 1108 8 0.02098438630460464 1108 9 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60.0 6 448.0 7 5082.0 8 13248.0 9 18772.0 10 21767.0 11 22390.0 12 24049.0 13 28162.0 14 35503.0 15 47518.0 16 62989.0 17 81740.0 18 90392.0 19 101161.0 20 115308.0 21 142786.0 22 191265.0 23 294291.0 24 450811.0 25 572610.0 26 560502.0 27 451186.0 28 370816.0 29 359937.0 30 397065.0 31 477209.0 32 589326.0 33 759315.0 34 1052529.0 35 1705362.0 36 2904718.0 37 5366992.0 38 6802124.0 39 1999719.0 40 3805.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.800693383291396 37.37085251895279 26.419755619832298 19.408698477923522 2 20.25803164209628 35.67684734419896 25.983791335893063 18.081329677811695 3 28.334132823774567 25.420581209817367 25.822422386682625 20.422863579725444 4 19.44430627161312 26.580373297932496 24.94118528241461 29.034135148039773 5 20.37213240281343 34.701080066467746 25.363282518643416 19.563505012075403 6 29.169741081859367 26.023106578717996 25.456169286905368 19.35098305251727 7 20.12636635826984 25.844957256184852 34.31642065643409 19.712255729111213 8 28.824478488125816 26.102659076620643 25.386387988858626 19.68647444639491 9 28.280460257874857 25.699265299046754 26.19690542976865 19.823369013309748 10-11 19.500830812195133 30.827086409081737 30.072355825642553 19.599726953080577 12-13 19.486260748600046 26.496560482444874 29.79691831571288 24.220260453242197 14-15 19.512897053389004 35.227304950056876 25.398613594020592 19.861184402533528 16-17 24.12934034261201 26.22099910947689 25.41006578380397 24.23959476410713 18-19 19.875189418911543 30.8341637668849 25.375133528888398 23.91551328531516 20-21 24.15894869458156 30.865949229178174 25.38742359745756 19.587678478782706 22-23 23.86047350070062 26.647388612130374 29.866290806510488 19.625847080658517 24-25 19.58408972470481 30.619338551779673 25.543610070787125 24.252961652728388 26-27 19.602441750843326 26.380807735602186 34.40367956941353 19.61307094414097 28-29 19.604802048969795 26.466101038509652 34.385769470759 19.54332744176156 30-31 19.599917391985024 26.4554331175823 34.33885313075226 19.605796359680415 32-33 19.638940524404568 26.529758663560017 34.224047653755655 19.607253158279764 34-35 19.640367820230274 26.63043880879428 34.15586097894696 19.573332392028483 36-37 19.619116969101945 26.607938886394084 34.15148976972476 19.621454374779205 38-39 19.65165379695106 26.607945173175594 34.13070556057059 19.609695469302757 40-41 19.695750889529286 26.676782994675076 34.070572080052735 19.556894035742904 42-43 19.720547252585146 26.721623508064507 34.01612252569735 19.541706713653003 44-45 19.77828687975099 26.774707343838184 33.91804427252528 19.528961503885554 46-47 19.736533792762298 26.901869549679898 33.86804021654758 19.493556441010227 48-49 19.824610289166518 26.91010484367684 33.73212874035314 19.5331561268035 50-51 19.878669886965906 26.992135207240402 33.59269265978198 19.53650224601171 52-53 20.010617950557524 27.17252801018037 33.29303476225515 19.52381927700696 54-55 20.44208869785705 27.517520406378214 32.25668163159606 19.783709264168678 56-57 21.02374423329067 28.243908949161078 30.732037483342662 20.000309334205593 58-59 21.246641345308458 28.907968233511454 29.880815844520807 19.96457457665928 60-61 21.385423667021545 29.250856718511308 29.423011242196996 19.94070837227015 62-63 21.513134698232843 29.447985683614036 29.168815008501625 19.870064609651497 64-65 21.64413660976077 29.518611883936202 29.115166077871095 19.722085428431928 66-67 21.750025683673353 29.283139437471373 29.337148369359994 19.629686509495283 68-69 21.859786695261967 28.968783360568924 29.687115788176072 19.48431415599304 70-71 22.0126320572132 28.76536244109362 29.924991250229798 19.29701425146338 72-73 22.18692099526962 28.648648959053634 29.94355225787619 19.220877787800553 74-75 22.43038103974334 28.729785340813425 29.715715057887536 19.124118561555704 76-77 22.737277082685065 28.917015327498717 29.290448153080202 19.055259436736012 78-79 23.095901611462658 29.10235622152884 28.784811320178648 19.016930846829858 80-81 23.430220704011816 29.307415535268806 28.312777403310808 18.94958635740857 82-83 23.616516626931585 29.510333377483782 27.924412800163623 18.948737195421018 84-85 23.824870586296683 29.547083432748828 27.687788464454073 18.94025751650042 86-87 24.015262041915925 29.536141444503112 27.539791584226947 18.908804929354012 88-89 24.215730207171458 29.53456146835634 27.394646033681802 18.855062290790404 90-91 24.462080401216475 29.492303238109823 27.20020864788038 18.84540771279332 92-93 24.63469620209162 29.491305861874167 27.085936962827944 18.78806097320627 94-95 24.828476697579696 29.487211169815904 26.967629802272974 18.716682330331423 96-97 24.986533495475026 29.45293756243213 26.81591458679519 18.74461435529765 98-99 25.11722359038805 29.445129803552284 26.706893855872792 18.730752750186873 100 25.198458826484355 29.48750199098238 26.580621768482445 18.73341741405082 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 109973.0 1 68624.5 2 22446.0 3 16662.0 4 15417.5 5 15483.0 6 15829.0 7 15457.5 8 15119.5 9 15239.0 10 15957.5 11 22838.0 12 209402.5 13 245762.5 14 95824.0 15 94842.0 16 105131.5 17 114898.0 18 123199.5 19 131821.5 20 142283.0 21 157777.5 22 180796.0 23 204181.5 24 224661.0 25 250045.0 26 281352.5 27 319063.0 28 365847.5 29 408662.0 30 442299.0 31 477839.5 32 518424.0 33 552841.0 34 579849.0 35 613798.5 36 651051.0 37 687029.5 38 729872.5 39 755535.5 40 762467.5 41 784966.5 42 815592.0 43 819447.5 44 808869.0 45 814134.0 46 823109.0 47 826030.5 48 823589.0 49 816906.5 50 810484.0 51 805143.0 52 792323.5 53 754837.5 54 702191.0 55 654960.5 56 609543.0 57 556372.5 58 493814.0 59 421243.5 60 344523.0 61 278020.5 62 218518.0 63 163076.0 64 118810.0 65 85501.0 66 61478.5 67 45577.5 68 34013.5 69 25481.5 70 19240.5 71 14988.0 72 12347.5 73 10026.5 74 8261.5 75 7160.0 76 5578.0 77 3602.5 78 2338.0 79 1709.0 80 1334.0 81 1033.5 82 778.5 83 622.5 84 540.5 85 447.0 86 353.0 87 297.5 88 257.5 89 218.0 90 187.5 91 151.5 92 122.5 93 112.5 94 90.5 95 72.5 96 63.5 97 46.0 98 35.5 99 24.0 100 18.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004597790564476222 2 1.0336415090829142E-4 3 0.08946358676206896 4 0.025285933953676478 5 0.01728861131488311 6 0.056088450924347326 7 0.037754713194724815 8 0.02560368301017233 9 0.005217975469925971 10-11 0.007356082072973406 12-13 0.014436526410191369 14-15 0.026359772632557055 16-17 0.049737298096315635 18-19 0.06279946412961528 20-21 0.045311781116686566 22-23 0.026489934896663795 24-25 0.018804618861538055 26-27 0.01719098961680306 28-29 0.012024696222331235 30-31 0.010378526411569557 32-33 0.007654689620041803 34-35 0.005633346224501882 36-37 0.009727715091035871 38-39 0.014017327353729963 40-41 0.015535249051327651 42-43 0.009354455657200373 44-45 0.0036617707534733605 46-47 0.0026817254707873386 48-49 0.0019007518861469143 50-51 0.0010489547166248833 52-53 0.0014624113202580489 54-55 2.2395566030129806E-4 56-57 0.002396516980318164 58-59 0.010380440562512304 60-61 0.022014649992523328 62-63 0.02721731225490733 64-65 0.00859262358198741 66-67 0.0021534198105894044 68-69 0.0015198358485404332 70-71 0.0021285358483337045 72-73 6.871801884458633E-4 74-75 6.163566035642563E-4 76-77 0.001324592452380327 78-79 0.0032215160366417493 80-81 0.007248889620179622 82-83 0.008485431129193628 84-85 0.007296743393748276 86-87 0.004387233960774147 88-89 0.002323779244493811 90-91 0.002601331131192001 92-93 0.002503709433111948 94-95 0.003106666980076981 96-97 0.0033612490554622175 98-99 0.007373309431458122 100 0.0133301471652841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6121242E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.282239738184785 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48551309942837 29.60161055954692 2 13.140309002032252 10.586421549073815 3 4.785684946622582 5.783343249938187 4 2.348516380593956 3.784139994885591 5 1.424354182388567 2.8688088323531216 6 0.9510443105292751 2.2986116951026148 7 0.6728944170550535 1.8973985958408428 8 0.48797297881525914 1.5725315614713946 9 0.37878915422730675 1.3732627968727757 >10 1.9658141971883007 14.51782343164035 >50 0.2080451647097963 6.080979982402191 >100 0.14857837203433918 9.968571268401503 >500 0.0016204515787738522 0.3887074556497272 >1k 6.92751159516456E-4 0.6503459019932901 >5k 1.1372775768960576E-4 0.2996492322681359 >10k+ 5.686387884385366E-5 8.327793892559459 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1958383 7.497281331416018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108752 0.4163354866510559 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 48148 0.18432507918268204 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38063 0.14571665466749245 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02563048112337078 3.828301885492275E-6 0.0 0.0 1.53132075419691E-5 2 0.025687905651653162 2.2969811312953648E-5 0.0 0.0 1.53132075419691E-5 3 0.02573001697239358 3.445471696943047E-5 0.0 3.828301885492275E-6 1.53132075419691E-5 4 0.025756815085592025 6.12528301678764E-5 0.0 3.828301885492275E-6 1.53132075419691E-5 5 0.02576064338747752 7.65660377098455E-5 0.0 3.828301885492275E-6 1.53132075419691E-5 6 0.025791269802561457 8.422264148083005E-5 0.0 3.828301885492275E-6 1.53132075419691E-5 7 0.025795098104446947 1.071924527937837E-4 0.0 3.828301885492275E-6 1.53132075419691E-5 8 0.025802754708217933 1.1102075467927597E-4 0.0 3.828301885492275E-6 1.53132075419691E-5 9 0.025829552821416378 1.1867735845026052E-4 0.0 3.828301885492275E-6 1.53132075419691E-5 10-11 0.02585252263272933 1.2824811316399122E-4 0.0 3.828301885492275E-6 1.53132075419691E-5 12-13 0.025896548104412494 1.3973301882046804E-4 0.0 3.828301885492275E-6 1.7227358484715236E-5 14-15 0.025957800934580368 1.588745282479294E-4 0.0 3.828301885492275E-6 1.9141509427461375E-5 16-17 0.025986513198721563 1.646169810761678E-4 0.0 3.828301885492275E-6 1.9141509427461375E-5 18-19 0.026019053764748244 1.741877357898985E-4 0.0 3.828301885492275E-6 2.1055660370207513E-5 20-21 0.026074564142087885 1.990716980455983E-4 0.0 3.828301885492275E-6 2.2969811312953648E-5 22-23 0.026239181123164052 2.1055660370207513E-4 0.0 3.828301885492275E-6 2.2969811312953648E-5 24-25 0.02696464433046484 2.2204150935855194E-4 0.0 3.828301885492275E-6 2.6798113198445924E-5 26-27 0.02911423583916875 2.3735471690052105E-4 0.0 3.828301885492275E-6 3.2540566026684335E-5 28-29 0.03528928678046779 2.545820753852363E-4 0.0 3.828301885492275E-6 3.636886791217661E-5 30-31 0.049283644322884804 2.7372358481269764E-4 0.0 3.828301885492275E-6 3.828301885492275E-5 32-33 0.07638419337028461 2.8903679235466674E-4 0.0 3.828301885492275E-6 4.019716979766889E-5 34-35 0.12385705090133156 3.024358489538897E-4 0.0 3.828301885492275E-6 4.2111320740415026E-5 36-37 0.19513237540542672 3.196632074386049E-4 0.0 3.828301885492275E-6 4.785377356865343E-5 38-39 0.2956004159373432 3.330622640378279E-4 0.0 3.828301885492275E-6 5.168207545414571E-5 40-41 0.4278988724961853 3.464613206370509E-4 0.0 3.828301885492275E-6 5.359622639689185E-5 42-43 0.5955746667788615 3.6177452817902E-4 0.0 3.828301885492275E-6 5.5510377339637986E-5 44-45 0.8028312742556423 3.6943113195000456E-4 1.9141509427461373E-6 3.828301885492275E-6 5.933867922513026E-5 46-47 1.0524729260576506 3.9814339609119657E-4 3.828301885492275E-6 7.65660377098455E-6 6.12528301678764E-5 48-49 1.346965048599144 4.096283017476734E-4 3.828301885492275E-6 7.65660377098455E-6 6.12528301678764E-5 50-51 1.6847112399938715 4.1728490551865797E-4 3.828301885492275E-6 1.53132075419691E-5 6.316698111062254E-5 52-53 2.065372695524968 4.3259811306062707E-4 3.828301885492275E-6 1.9141509427461375E-5 6.508113205336867E-5 54-55 2.4864973878347745 4.3834056588886545E-4 3.828301885492275E-6 1.9141509427461375E-5 6.69952829961148E-5 56-57 2.952685021638711 4.498254715453423E-4 3.828301885492275E-6 2.1055660370207513E-5 7.082358488160709E-5 58-59 3.460775333730303 4.632245281445653E-4 3.828301885492275E-6 2.2969811312953648E-5 7.273773582435322E-5 60-61 4.004355918451351 4.6896698097280366E-4 3.828301885492275E-6 2.6798113198445924E-5 8.61367924235762E-5 62-63 4.587228279574148 4.823660375720266E-4 3.828301885492275E-6 2.6798113198445924E-5 9.187924525181459E-5 64-65 5.203726530308169 4.88108490400265E-4 3.828301885492275E-6 2.871226414119206E-5 9.187924525181459E-5 66-67 5.854040171596742 5.01507546999488E-4 3.828301885492275E-6 3.06264150839382E-5 9.187924525181459E-5 68-69 6.535634867591671 5.072499998277265E-4 3.828301885492275E-6 3.06264150839382E-5 9.570754713730687E-5 70-71 7.24076787772955 5.263915092551878E-4 3.828301885492275E-6 3.06264150839382E-5 9.570754713730687E-5 72-73 7.971910370877465 5.43618867739903E-4 3.828301885492275E-6 3.636886791217661E-5 9.570754713730687E-5 74-75 8.727220933828491 5.551037733963799E-4 3.828301885492275E-6 3.828301885492275E-5 9.570754713730687E-5 76-77 9.505183559035975 5.627603771673644E-4 3.828301885492275E-6 3.828301885492275E-5 9.570754713730687E-5 78-79 10.301816812539005 5.799877356520796E-4 3.828301885492275E-6 3.828301885492275E-5 9.570754713730687E-5 80-81 11.115798016036145 5.953009431940487E-4 3.828301885492275E-6 4.019716979766889E-5 9.570754713730687E-5 82-83 11.948476645942026 6.163566035642563E-4 3.828301885492275E-6 4.2111320740415026E-5 9.762169808005301E-5 84-85 12.794774459805549 6.27841509220733E-4 3.828301885492275E-6 4.2111320740415026E-5 1.0336415090829142E-4 86-87 13.652746680268878 6.41240565819956E-4 3.828301885492275E-6 4.402547168316116E-5 1.071924527937837E-4 88 14.30515057438693 6.584679243046712E-4 3.828301885492275E-6 4.976792451139957E-5 1.071924527937837E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 248395 0.0 86.626625 5 AAGCAGT 253365 0.0 86.609955 1 GGTATCA 247435 0.0 86.49644 8 GTGGTAT 249765 0.0 85.89724 6 TGGTATC 249055 0.0 85.80902 7 GTATCAA 250745 0.0 84.64311 9 GCAGTGG 258165 0.0 84.59698 3 CAGTGGT 258260 0.0 83.74663 4 AGCAGTG 264885 0.0 83.02466 2 CAACGCA 245885 0.0 45.351208 12-13 ATCAACG 246045 0.0 45.279125 10-11 ACGCAGA 249865 0.0 44.939888 14-15 AGAGTAC 250445 0.0 44.53077 18-19 AGTACTT 253200 0.0 43.69867 20-21 TCAACGC 245790 0.0 43.11809 12-13 AACGCAG 247435 0.0 42.65144 14-15 TATCAAC 249390 0.0 42.477615 10-11 GAGTACT 246445 0.0 41.894 20-21 CGCAGAG 251480 0.0 41.888737 16-17 GCAGAGT 268855 0.0 41.568024 16-17 >>END_MODULE