FastQCFastQC Report
Thu 26 May 2016
SRR1660702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26121242
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19340617.404169372957075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1003530.38418157911480627No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT501110.19184003578390338No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452770.17333402446943375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2468050.085.692655
AAGCAGT2496200.085.65141
GGTATCA2451400.085.644298
GTGGTAT2479600.085.012346
TGGTATC2468550.084.971687
GTATCAA2479000.083.936549
GCAGTGG2553400.083.7890553
CAGTGGT2570500.082.799934
AGCAGTG2625250.081.984432
CAACGCA2430200.044.88981212-13
ATCAACG2426550.044.80168510-11
ACGCAGA2472100.044.47126414-15
AGAGTAC2492950.044.0015118-19
AGTACTT2519950.043.2427220-21
TCAACGC2427150.042.74559412-13
AACGCAG2447200.042.22480414-15
TATCAAC2461100.042.1881410-11
GAGTACT2448000.041.59653520-21
CGCAGAG2482900.041.54821816-17
GCAGAGT2674800.041.19178416-17
Produced by FastQC (version 0.11.5)