FastQCFastQC Report
Thu 26 May 2016
SRR1660701_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660701_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49218863
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17297723.5144493280960183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2004980.40736008062599904No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT848470.17238715977652713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2210750.085.098238
GTGGTAT2256100.083.5575566
TGGTATC2245150.083.517197
AGTGGTA2267650.083.3973855
GTATCAA2275200.081.982289
GCAGTGG2432550.079.0395663
AAGCAGT2459350.078.623221
CAGTGGT2459700.077.324694
AGCAGTG2580350.075.1341862
CAACGCA2158650.045.12844512-13
ATCAACG2187950.044.52528410-11
ACGCAGA2221550.044.12738814-15
TCAACGC2159650.043.1644812-13
AGAGTAC2259200.043.10405718-19
AACGCAG2186300.042.44632314-15
TATCAAC2240400.041.53988310-11
CGCAGAG2237200.041.35767416-17
GAGTACT2230600.040.73486720-21
AGTACTT2403600.040.38261420-21
GCAGAGT2542300.038.39546216-17
Produced by FastQC (version 0.11.5)