FastQCFastQC Report
Thu 26 May 2016
SRR1660701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49218863
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17220123.498683015087122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1904810.38700812735149936No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT947360.1924790501560347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2213050.084.014448
GTGGTAT2260250.082.4951556
TGGTATC2253400.082.410747
AGTGGTA2284250.081.939075
GTATCAA2275900.081.0122459
GCAGTGG2423550.078.167643
AAGCAGT2442700.077.6343151
CAGTGGT2467750.076.333024
AGCAGTG2568600.074.247222
CAACGCA2155800.044.56490312-13
ATCAACG2176550.044.0479510-11
ACGCAGA2219650.043.59487514-15
TCAACGC2151900.042.6966912-13
AGAGTAC2273700.042.4593918-19
AACGCAG2177900.041.9632314-15
TATCAAC2240000.041.11838510-11
CGCAGAG2235000.040.80999816-17
GAGTACT2237100.040.29360220-21
AGTACTT2418750.039.84284620-21
GCAGAGT2571050.037.72823716-17
Produced by FastQC (version 0.11.5)