FastQCFastQC Report
Thu 26 May 2016
SRR1660700_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660700_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28573444
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16279425.69739510574924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070530.37465907154909295No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT380580.13319360452313694No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347710.12168991599332583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2162500.084.737448
AGTGGTA2173000.084.735865
GTGGTAT2179500.084.256726
TGGTATC2175050.084.010527
AAGCAGT2252850.083.710271
GTATCAA2184600.083.114739
GCAGTGG2291000.081.6713643
CAGTGGT2276400.081.332924
AGCAGTG2343450.080.593332
CAACGCA2138500.044.7376612-13
ATCAACG2150300.044.52125510-11
ACGCAGA2177250.044.19981414-15
AGAGTAC2183600.043.69666718-19
AGTACTT2173100.042.76746720-21
TCAACGC2141550.042.40099712-13
AACGCAG2154050.041.9363614-15
TATCAAC2182900.041.49662410-11
GAGTACT2099950.041.2173120-21
CGCAGAG2198250.040.9715916-17
GCAGAGT2340900.040.86530316-17
Produced by FastQC (version 0.11.5)