FastQCFastQC Report
Thu 26 May 2016
SRR1660700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28573444
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16200895.669911544439656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA982160.34373175316213195No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT402930.14101555276290811No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395030.13825074779225074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2139250.084.172198
AGTGGTA2164750.083.796185
GTGGTAT2164600.083.5424356
TGGTATC2157900.083.398947
AAGCAGT2222850.082.885481
GTATCAA2163400.082.435629
GCAGTGG2272050.080.9117663
CAGTGGT2272950.080.377954
AGCAGTG2312150.080.001762
CAACGCA2115000.044.31634512-13
ATCAACG2117100.044.15407610-11
ACGCAGA2163400.043.75381514-15
AGAGTAC2172150.043.4094118-19
AGTACTT2159850.042.562820-21
TCAACGC2112950.042.09071712-13
AACGCAG2130850.041.57703814-15
TATCAAC2158750.041.2451810-11
GAGTACT2084900.041.04871420-21
CGCAGAG2177650.040.64404716-17
GCAGAGT2336850.040.50642416-17
Produced by FastQC (version 0.11.5)