FastQCFastQC Report
Thu 26 May 2016
SRR1660699_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660699_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24919383
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18582127.456894097257545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1079060.43302035206890954No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT454580.18242024692184394No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425090.1705860855383137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2359700.086.341575
GGTATCA2350650.086.2089168
AAGCAGT2427350.085.631311
GTGGTAT2377650.085.3990866
TGGTATC2371100.085.260167
GTATCAA2379950.084.3794259
GCAGTGG2449850.084.320493
CAGTGGT2468400.082.942324
AGCAGTG2534800.082.157482
CAACGCA2327850.045.3367712-13
ATCAACG2338050.045.19023510-11
ACGCAGA2369200.044.84106414-15
AGAGTAC2375350.044.43908718-19
AGTACTT2419150.043.35434720-21
TCAACGC2330900.043.02426512-13
AACGCAG2347850.042.5162114-15
TATCAAC2364850.042.3741810-11
GAGTACT2329350.041.90776420-21
CGCAGAG2374300.041.9039516-17
GCAGAGT2521450.041.89198716-17
Produced by FastQC (version 0.11.5)