FastQCFastQC Report
Thu 26 May 2016
SRR1660699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24919383
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18273927.3332152726253295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA997230.4001824603763264No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494680.1985121381215578No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT476760.19132094883729667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2310750.085.483798
AGTGGTA2330100.085.481235
AAGCAGT2373150.084.87481
TGGTATC2333800.084.605547
GTGGTAT2351200.084.425566
GTATCAA2344700.083.58199
GCAGTGG2418450.083.3484043
CAGTGGT2446650.081.918964
AGCAGTG2492100.081.3472752
CAACGCA2288600.044.87842612-13
ATCAACG2290350.044.76479310-11
ACGCAGA2330950.044.44503814-15
AGAGTAC2353450.043.9196918-19
AGTACTT2389050.043.05634720-21
TCAACGC2289150.042.59162512-13
AACGCAG2304300.042.12945614-15
TATCAAC2329100.041.959210-11
GAGTACT2309000.041.47158420-21
GCAGAGT2501400.041.45211416-17
CGCAGAG2339150.041.43929316-17
Produced by FastQC (version 0.11.5)