FastQCFastQC Report
Thu 26 May 2016
SRR1660698_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660698_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29672176
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15839815.338270438945901No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1339590.4514633507161726No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT495990.1671565981544461No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT332660.11211176423326688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2028850.086.0946358
AGTGGTA2045750.085.905975
TGGTATC2046400.085.1998757
GTGGTAT2087500.083.987616
GTATCAA2073400.083.5854649
AAGCAGT2149750.083.376251
GCAGTGG2148850.082.903373
AGCAGTG2226200.080.6707462
CAGTGGT2193650.080.539954
CAACGCA2000850.045.34858312-13
ATCAACG2011300.045.10802510-11
ACGCAGA2044350.044.7099314-15
AGAGTAC2056100.044.10996618-19
TCAACGC2005450.043.19820812-13
AACGCAG2023250.042.6441714-15
TATCAAC2049300.042.19478210-11
AGTACTT2146850.042.05109820-21
CGCAGAG2053050.041.9266316-17
GAGTACT2026900.041.65464420-21
CAGAGTA2146750.039.71673218-19
Produced by FastQC (version 0.11.5)