##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660698_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29672176 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.230599299491885 34.0 31.0 34.0 30.0 34.0 2 32.374916757031905 34.0 31.0 34.0 30.0 34.0 3 32.358754713506684 34.0 31.0 34.0 30.0 34.0 4 35.77295918573683 37.0 35.0 37.0 35.0 37.0 5 35.78734785072723 37.0 35.0 37.0 35.0 37.0 6 35.802377621378355 37.0 36.0 37.0 35.0 37.0 7 35.72293969946794 37.0 35.0 37.0 35.0 37.0 8 35.78203428693602 37.0 35.0 37.0 35.0 37.0 9 37.47587187404119 39.0 38.0 39.0 35.0 39.0 10-11 37.36816892027063 39.0 37.0 39.0 34.5 39.0 12-13 37.43778818243731 39.0 38.0 39.0 35.0 39.0 14-15 38.86178175810227 41.0 39.0 41.0 35.0 41.0 16-17 38.81018833940591 40.5 39.0 41.0 35.0 41.0 18-19 38.71660720130535 40.0 38.5 41.0 35.0 41.0 20-21 38.64073071351423 40.0 38.5 41.0 34.5 41.0 22-23 38.455621421226404 40.0 38.0 41.0 34.0 41.0 24-25 38.468543055285195 40.0 38.0 41.0 34.0 41.0 26-27 38.45259990369429 40.0 38.0 41.0 34.0 41.0 28-29 38.38439011011528 40.0 38.0 41.0 34.0 41.0 30-31 38.27520226356167 40.0 38.0 41.0 34.0 41.0 32-33 38.15668313304693 40.0 38.0 41.0 33.0 41.0 34-35 38.0328318691558 40.0 38.0 41.0 33.0 41.0 36-37 37.83894357798363 40.0 38.0 41.0 33.0 41.0 38-39 37.538201579823465 40.0 36.0 41.0 33.0 41.0 40-41 37.56866589427078 40.0 36.0 41.0 33.0 41.0 42-43 37.47996965237736 40.0 36.0 41.0 33.0 41.0 44-45 37.33743344269729 40.0 36.0 41.0 32.0 41.0 46-47 37.36915536629333 40.0 36.0 41.0 32.0 41.0 48-49 37.29859913206231 40.0 36.0 41.0 32.0 41.0 50-51 35.912701110966715 38.5 34.0 40.0 30.0 40.5 52-53 36.09276532331164 38.5 35.0 39.5 30.0 40.5 54-55 36.52363390875007 39.5 35.0 41.0 29.0 41.0 56-57 36.18744284207535 40.0 35.0 41.0 27.5 41.0 58-59 35.99189193269817 39.0 35.0 41.0 27.0 41.0 60-61 35.81298051750569 39.0 35.0 41.0 26.5 41.0 62-63 35.59498403487496 39.0 35.0 41.0 26.0 41.0 64-65 35.33297338894188 38.5 35.0 41.0 26.0 41.0 66-67 35.06425662883639 37.5 35.0 40.0 26.0 41.0 68-69 34.791957505913956 37.0 34.0 40.0 26.0 41.0 70-71 34.291427952570785 36.5 34.0 39.5 25.0 41.0 72-73 33.98001784567468 36.0 34.0 39.0 24.5 41.0 74-75 33.57917349910569 35.5 34.0 39.0 24.0 40.5 76-77 33.23313686195445 35.0 33.0 37.5 23.5 40.0 78-79 32.861043692919594 35.0 33.0 37.0 22.5 39.0 80-81 32.487693757276176 35.0 33.0 36.5 21.5 39.0 82-83 32.18749398426324 35.0 33.0 36.0 20.0 38.0 84-85 31.89116187501719 35.0 33.0 36.0 20.0 37.0 86-87 31.626372430522117 35.0 32.0 35.0 19.5 37.0 88-89 31.436408809384254 35.0 32.0 35.0 19.0 36.0 90-91 31.240240048454822 35.0 32.0 35.0 19.0 36.0 92-93 31.075762660615116 35.0 32.0 35.0 18.0 36.0 94-95 30.90303793358465 35.0 31.5 35.0 18.0 36.0 96-97 30.77319674836116 35.0 31.0 35.0 17.5 35.0 98-99 30.65240029918938 35.0 31.0 35.0 17.0 35.0 100 30.554249813023485 35.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33875946027412596 1101 2 0.32289653695760023 1101 3 0.3143421043883379 1101 4 0.29348338306520105 1101 5 0.30855793022969635 1101 6 0.2452884676083542 1101 7 0.2772610773918629 1101 8 0.26996341714656324 1101 9 0.36308424994548716 1101 10-11 0.33021486606014605 1101 12-13 0.36624725106825196 1101 14-15 0.4366947166917754 1101 16-17 0.4087525972653694 1101 18-19 0.45632745124523666 1101 20-21 0.4191312176639457 1101 22-23 0.4032369506250433 1101 24-25 0.4109429522033281 1101 26-27 0.44844216420828786 1101 28-29 0.42841658135110094 1101 30-31 0.4234543713266632 1101 32-33 0.40868671851018945 1101 34-35 0.4375445941288518 1101 36-37 0.42802357441701844 1101 38-39 0.4186139689515187 1101 40-41 0.4070534468391429 1101 42-43 0.3818384183014274 1101 44-45 0.36381923820636786 1101 46-47 0.37377793329371656 1101 48-49 0.3540282943915898 1101 50-51 0.32635083299020806 1101 52-53 0.44219918011760484 1101 54-55 0.4808838267957256 1101 56-57 0.4797826223520474 1101 58-59 0.4685897291962391 1101 60-61 0.46095784275518525 1101 62-63 0.4530607765850476 1101 64-65 0.4585286803358599 1101 66-67 0.44036507584733187 1101 68-69 0.4506456193602304 1101 70-71 0.39738769829866527 1101 72-73 0.401399910595039 1101 74-75 0.34659449505393525 1101 76-77 0.33046220751008093 1101 78-79 0.3820284022470517 1101 80-81 0.4368566503353293 1101 82-83 0.3506963609042373 1101 84-85 0.35177591509051354 1101 86-87 0.3455929805529081 1101 88-89 0.31734095044169663 1101 90-91 0.2794916800275473 1101 92-93 0.25963081571376634 1101 94-95 0.2638427924105713 1101 96-97 0.2845064947006364 1101 98-99 0.2589346658624265 1101 100 0.21041512212795155 1102 1 0.3224167825586761 1102 2 0.30122130809137815 1102 3 0.33395119282368313 1102 4 0.27702596614784625 1102 5 0.25927626116569513 1102 6 0.2102071004493382 1102 7 0.21997207183488143 1102 8 0.22900823639614032 1102 9 0.2966980167937763 1102 10-11 0.3080592719171804 1102 12-13 0.26775127899640694 1102 14-15 0.3313881445998774 1102 16-17 0.340189087863358 1102 18-19 0.3730386161681025 1102 20-21 0.3639498187939054 1102 22-23 0.3080891727673887 1102 24-25 0.3146797398738599 1102 26-27 0.3530805345894592 1102 28-29 0.3110457088514238 1102 30-31 0.31864744456187566 1102 32-33 0.3370676056430071 1102 34-35 0.30343054241168943 1102 36-37 0.30839161224122336 1102 38-39 0.3079790572109715 1102 40-41 0.2841364536012847 1102 42-43 0.26809614570434803 1102 44-45 0.28521909548928903 1102 46-47 0.29500683079162116 1102 48-49 0.30941115313896717 1102 50-51 0.27162179384576035 1102 52-53 0.33109221589265303 1102 54-55 0.4307165338627428 1102 56-57 0.3631554239010413 1102 58-59 0.385735695757802 1102 60-61 0.3369223056625401 1102 62-63 0.311322015223908 1102 64-65 0.3558803328780158 1102 66-67 0.35488653245953117 1102 68-69 0.3492198362640835 1102 70-71 0.32832890123923164 1102 72-73 0.25411497612550704 1102 74-75 0.1974023488619423 1102 76-77 0.16054917696742876 1102 78-79 0.20961840664352138 1102 80-81 0.25468753892763374 1102 82-83 0.22885010690750107 1102 84-85 0.17246702956140325 1102 86-87 0.19175605011526642 1102 88-89 0.172611375370316 1102 90-91 0.16218416140132774 1102 92-93 0.14294170943216145 1102 94-95 0.15653366200152874 1102 96-97 0.1253343211275677 1102 98-99 0.08603308759778372 1102 100 0.08980200971019414 1103 1 0.24459804416113684 1103 2 0.2068702007421166 1103 3 0.2491631130805274 1103 4 0.21575883112932814 1103 5 0.20291602760612193 1103 6 0.17338434497493438 1103 7 0.1751662657075741 1103 8 0.19589527828594555 1103 9 0.2453698663241255 1103 10-11 0.23149782431174515 1103 12-13 0.23825046021483587 1103 14-15 0.27965921489002454 1103 16-17 0.2643704631085697 1103 18-19 0.3052490056331081 1103 20-21 0.3021650291175533 1103 22-23 0.29380287934625926 1103 24-25 0.2646629054145748 1103 26-27 0.25495533750216737 1103 28-29 0.2278250852304069 1103 30-31 0.22356717277664728 1103 32-33 0.2320006552809204 1103 34-35 0.23018697126073562 1103 36-37 0.2317970620634071 1103 38-39 0.24635699313130033 1103 40-41 0.24340829566375533 1103 42-43 0.2185320213638562 1103 44-45 0.24392533985739817 1103 46-47 0.25795001322126865 1103 48-49 0.2171093623335949 1103 50-51 0.18536541154944786 1103 52-53 0.29244591013232224 1103 54-55 0.38420724551230734 1103 56-57 0.395878678463248 1103 58-59 0.35748677851213984 1103 60-61 0.33334502160030866 1103 62-63 0.33484520324866196 1103 64-65 0.3499788031320179 1103 66-67 0.3677337203195634 1103 68-69 0.3400401358087848 1103 70-71 0.2646713198387616 1103 72-73 0.2247623129768357 1103 74-75 0.23069106623889013 1103 76-77 0.21997674140971668 1103 78-79 0.266142304028385 1103 80-81 0.26719313524628774 1103 82-83 0.20624565737158917 1103 84-85 0.23080957784101486 1103 86-87 0.21590355219561275 1103 88-89 0.1927788443128442 1103 90-91 0.15827408780483765 1103 92-93 0.20777038316953522 1103 94-95 0.2038392188831537 1103 96-97 0.12014510623986396 1103 98-99 0.0774403365777907 1103 100 0.08451816120309985 1104 1 0.1704261289062785 1104 2 0.1502092072613408 1104 3 0.17564172552467738 1104 4 0.15093588940019487 1104 5 0.12920017879836365 1104 6 0.1123871005617616 1104 7 0.10585728251647453 1104 8 0.12254154622333147 1104 9 0.17455383475574138 1104 10-11 0.1718593756794249 1104 12-13 0.15386564927963065 1104 14-15 0.19588198244671418 1104 16-17 0.2002509186469723 1104 18-19 0.2049372775348175 1104 20-21 0.17217906084526646 1104 22-23 0.17676001006039854 1104 24-25 0.2040873671129404 1104 26-27 0.22595013841672085 1104 28-29 0.16113262853369292 1104 30-31 0.1520407193272817 1104 32-33 0.16483900774836258 1104 34-35 0.18894974211016802 1104 36-37 0.12301840734369307 1104 38-39 0.1350498315707256 1104 40-41 0.1595665469259444 1104 42-43 0.12398181679166242 1104 44-45 0.07169282602225735 1104 46-47 0.1156703735544582 1104 48-49 0.08649387508297224 1104 50-51 0.05293206517956861 1104 52-53 0.12420354369350406 1104 54-55 0.1406896630662473 1104 56-57 0.20134161823136054 1104 58-59 0.19093815791130453 1104 60-61 0.19443415239111772 1104 62-63 0.17346882285133347 1104 64-65 0.1880604218496913 1104 66-67 0.199136224339874 1104 68-69 0.1547581319605129 1104 70-71 0.11822743597510055 1104 72-73 0.10493087582290883 1104 74-75 0.11074262701247051 1104 76-77 0.1320550389871329 1104 78-79 0.14394120012187983 1104 80-81 0.18443549288562622 1104 82-83 0.12550167795613731 1104 84-85 0.1062388078746963 1104 86-87 0.12343052097173057 1104 88-89 0.022677755456054882 1104 90-91 0.03214711240784851 1104 92-93 0.018899857617785898 1104 94-95 0.07326744010753217 1104 96-97 0.08523778536659776 1104 98-99 0.09412251814933725 1104 100 0.07168862195147341 1105 1 0.16490912691183013 1105 2 0.15090281700858554 1105 3 0.17564427686327377 1105 4 0.1767650271989396 1105 5 0.15111827988429383 1105 6 0.11302263979504801 1105 7 0.12898140687186554 1105 8 0.11485715058820034 1105 9 0.15830986741161723 1105 10-11 0.15571405615733624 1105 12-13 0.1529696805836025 1105 14-15 0.18130329900672137 1105 16-17 0.16286257317745623 1105 18-19 0.20786597998385048 1105 20-21 0.1934469721871963 1105 22-23 0.16934236711299633 1105 24-25 0.15291632631797114 1105 26-27 0.1997723314560318 1105 28-29 0.1845430185924215 1105 30-31 0.18685528189555356 1105 32-33 0.18276460646249149 1105 34-35 0.2055189637849537 1105 36-37 0.18786858195289113 1105 38-39 0.1957433657227554 1105 40-41 0.18660792688199734 1105 42-43 0.18013855906615106 1105 44-45 0.16592430456393004 1105 46-47 0.2152427743231371 1105 48-49 0.17407774837089818 1105 50-51 0.1594224599409486 1105 52-53 0.2233824201690311 1105 54-55 0.2722710614769852 1105 56-57 0.28446706668424326 1105 58-59 0.31606732139667315 1105 60-61 0.29889004951106557 1105 62-63 0.3172323295906452 1105 64-65 0.33354360167748354 1105 66-67 0.3061726272170233 1105 68-69 0.27380517785689307 1105 70-71 0.2800062359419613 1105 72-73 0.26623360899950654 1105 74-75 0.27636825697261713 1105 76-77 0.2354366335674598 1105 78-79 0.25432280163176557 1105 80-81 0.2762884249878823 1105 82-83 0.2716711521189552 1105 84-85 0.2871205508893837 1105 86-87 0.2770828842357318 1105 88-89 0.21013078405246688 1105 90-91 0.252326875867265 1105 92-93 0.2457779715895434 1105 94-95 0.24085087399825156 1105 96-97 0.266915030245336 1105 98-99 0.2404787203747354 1105 100 0.1694237695385361 1106 1 0.10070842868076824 1106 2 0.09286211992951365 1106 3 0.09276581770755143 1106 4 0.08552275510002261 1106 5 0.06661080158001198 1106 6 0.04827138861106306 1106 7 0.06086147880694881 1106 8 0.08987731033015933 1106 9 0.10500206975142845 1106 10-11 0.08285770871858489 1106 12-13 0.07820331759305788 1106 14-15 0.10085680872179381 1106 16-17 0.1265208783511511 1106 18-19 0.14175114625371776 1106 20-21 0.1155143297250163 1106 22-23 0.15233498480748864 1106 24-25 0.11375310760717383 1106 26-27 0.1726078791610206 1106 28-29 0.12389531488302907 1106 30-31 0.11988781907908219 1106 32-33 0.10775292661198677 1106 34-35 0.09492577937771784 1106 36-37 0.1263443694628137 1106 38-39 0.09231784956936195 1106 40-41 0.11374789949034891 1106 42-43 0.1114661580728793 1106 44-45 0.12005957163250969 1106 46-47 0.1403652927549004 1106 48-49 0.11543797895995311 1106 50-51 0.07731379093191038 1106 52-53 0.10630443876718942 1106 54-55 0.16568114582791083 1106 56-57 0.1638354907684132 1106 58-59 0.17685018337603964 1106 60-61 0.15670746944808656 1106 62-63 0.1778653534879311 1106 64-65 0.13688775887227678 1106 66-67 0.1508674951227178 1106 68-69 0.16359686852324984 1106 70-71 0.14279515224383488 1106 72-73 0.13266931862242615 1106 74-75 0.10003799399998314 1106 76-77 0.07307735176626551 1106 78-79 0.1584601886732102 1106 80-81 0.1783391987379872 1106 82-83 0.15377998112593616 1106 84-85 0.19741113916985853 1106 86-87 0.21111368864143287 1106 88-89 0.148643971083672 1106 90-91 0.18805069860233203 1106 92-93 0.16491316665904776 1106 94-95 0.19104163155655485 1106 96-97 0.15459730791218007 1106 98-99 0.1097494737090905 1106 100 0.060845559339625765 1107 1 0.05347026709721092 1107 2 0.05887850007376727 1107 3 0.05852531080523704 1107 4 0.08606764249298493 1107 5 0.07208591434572753 1107 6 0.07401543386883702 1107 7 0.03236887687695145 1107 8 0.0326876316039133 1107 9 0.056847808373589714 1107 10-11 0.0674553856371034 1107 12-13 0.07161463077621022 1107 14-15 0.04874161049186654 1107 16-17 0.048600074877100496 1107 18-19 0.08928312245526371 1107 20-21 0.08230630394758265 1107 22-23 0.09326259357558087 1107 24-25 0.07697735795768779 1107 26-27 0.06615802755055711 1107 28-29 0.08946465711447615 1107 30-31 0.06722235844767965 1107 32-33 0.04856394587901747 1107 34-35 0.08461968943789344 1107 36-37 0.07257802382340373 1107 38-39 0.09031211298979969 1107 40-41 0.08696655010181331 1107 42-43 0.02660825589537552 1107 44-45 0.0935810564910895 1107 46-47 0.09040467299555388 1107 48-49 0.03212165635120812 1107 50-51 -0.003637074174072552 1107 52-53 0.03465329734554956 1107 54-55 0.1213941399095404 1107 56-57 0.1541491664404262 1107 58-59 0.14526369568146436 1107 60-61 0.11765508559767568 1107 62-63 0.1116374833662519 1107 64-65 0.1460184715192412 1107 66-67 0.17499120220161046 1107 68-69 0.15689453933792663 1107 70-71 0.13628094601685348 1107 72-73 0.10355655111260376 1107 74-75 0.0982761490156463 1107 76-77 0.14034409548376203 1107 78-79 0.12391585579026554 1107 80-81 0.15912921542830105 1107 82-83 0.11092105274751418 1107 84-85 0.1336304767345382 1107 86-87 0.16027294983440044 1107 88-89 0.11847955907011709 1107 90-91 0.12959611056619025 1107 92-93 0.15185706285366862 1107 94-95 0.15884385242586063 1107 96-97 0.1585506711013096 1107 98-99 0.11658249419104649 1107 100 0.11021744024418467 1108 1 3.414531589385206E-5 1108 2 -0.014301548211264503 1108 3 0.04555902248254995 1108 4 0.07594556856509627 1108 5 0.05902992828175968 1108 6 0.0395626211549569 1108 7 0.013177522152695076 1108 8 0.03637716634545285 1108 9 0.051348019884954965 1108 10-11 0.022005286886447095 1108 12-13 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-0.8769231573768579 2316 50-51 -0.7475061657396012 2316 52-53 -0.9307201992991097 2316 54-55 -0.9208225933976522 2316 56-57 -1.1392345175430876 2316 58-59 -1.031424338041262 2316 60-61 -0.9576550745780281 2316 62-63 -0.9289405712057146 2316 64-65 -0.8016784324879822 2316 66-67 -0.7023069375973563 2316 68-69 -0.697671004886395 2316 70-71 -0.5960444538743559 2316 72-73 -0.6601522281989816 2316 74-75 -0.6205185507144861 2316 76-77 -0.7188060766468212 2316 78-79 -0.5972123338689599 2316 80-81 -0.7151023228126263 2316 82-83 -0.9011139547278475 2316 84-85 -1.0693997818797207 2316 86-87 -0.987418149915662 2316 88-89 -0.7662557425424623 2316 90-91 -0.7955314573639356 2316 92-93 -0.82119818159196 2316 94-95 -0.801507888531173 2316 96-97 -0.6940070306789252 2316 98-99 -0.794863268770591 2316 100 -0.8704873618705236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26.0 3 106.0 4 52.0 5 45.0 6 384.0 7 4240.0 8 10902.0 9 17057.0 10 21267.0 11 23478.0 12 25506.0 13 29619.0 14 37285.0 15 49162.0 16 64473.0 17 81136.0 18 90509.0 19 102358.0 20 116405.0 21 142083.0 22 188534.0 23 275760.0 24 405242.0 25 514604.0 26 512708.0 27 443650.0 28 376816.0 29 382357.0 30 437685.0 31 530715.0 32 661072.0 33 852635.0 34 1179983.0 35 1891077.0 36 3212498.0 37 6162232.0 38 8276098.0 39 2547749.0 40 4668.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.965526059836048 35.74326392251424 27.229346219316025 20.061863798333686 2 20.282989061003803 33.2693529184199 27.940984024016362 18.506673996559933 3 25.263945143031023 26.81101582386109 26.851247693774067 21.07379133933382 4 19.08123569790194 27.502121603929535 26.825247621017713 26.591395077150814 5 20.25533855463534 33.14834129875969 26.741674642204643 19.854645504400324 6 26.393867209149104 26.806679476241168 26.990283452376428 19.8091698622333 7 20.026275327243212 27.083189224560638 33.01427819606964 19.876257252126507 8 25.982857548115142 26.893854512840594 27.120519221787532 20.002768717256732 9 25.737117601044257 26.58157771365961 27.681648456350004 19.99965622894613 10-11 19.58074663927292 30.383526231966627 30.234171818596543 19.801555310163906 12-13 19.645506054599693 27.3254902722138 29.921056387110408 23.107947286076104 14-15 19.66235433082704 33.62455313872956 26.718835397635154 19.994257132808244 16-17 22.976498638411694 27.174385806126473 26.719027888333663 23.130087667128173 18-19 19.957045283249787 30.454686828068695 26.651610629088275 22.93665725959325 20-21 23.047116906227828 30.43661722287998 26.642532633308818 19.873733237583373 22-23 22.828535332918353 27.431914287858184 29.846481897463672 19.893068481759794 24-25 19.79789731705301 30.290838192040866 26.709461550797144 23.201802940108987 26-27 19.83540774535013 27.260937872397395 33.0241256296639 19.87952875258857 28-29 19.835959341153814 27.360055550356645 32.989010169362764 19.81497493912678 30-31 19.875023835377945 27.344124211747307 32.927456274141356 19.8533956787334 32-33 19.88447893275402 27.398152806163083 32.83341848082274 19.883949780260156 34-35 19.90497693726564 27.44555377390566 32.784480048382264 19.86498924044643 36-37 19.920894695487128 27.44736333206238 32.73790553791902 19.89383643453147 38-39 19.95488573751114 27.434659635664733 32.720500314427326 19.889954312396803 40-41 19.984856184650397 27.49398961184624 32.68350605632958 19.83764814717379 42-43 19.9733332609808 27.547392916665775 32.624132466880305 19.85514135547312 44-45 20.04759169629084 27.592458712523243 32.5336607319902 19.826288859195714 46-47 20.048736574500364 27.679755302554643 32.47111100699474 19.800397115950258 48-49 20.10529307800905 27.68782222030462 32.370920375670146 19.835964326016185 50-51 20.149031020781056 27.751004876358127 32.26200229587309 19.837961806987728 52-53 20.266507912308622 27.894415723099215 32.018682512530404 19.82039385206176 54-55 20.587163336280977 28.14026301084029 31.285336944858265 19.98723670802047 56-57 21.022483109949093 28.635851552358503 30.192621246143453 20.149044091548948 58-59 21.22160607648022 29.08306196879817 29.597676789021403 20.0976551657002 60-61 21.365586901695636 29.325310331775896 29.258869056532205 20.050233709996267 62-63 21.457811104249753 29.47467858533927 29.082325384427694 19.985184925983287 64-65 21.59564418110655 29.543899298367414 28.99429505660054 19.866161463925497 66-67 21.72652693782563 29.39658824994823 29.106938705016827 19.769946107209314 68-69 21.82554717922235 29.19790517179168 29.303200038371163 19.6733476106148 70-71 21.963828650203276 29.06945797175776 29.43332730109057 19.533386076948386 72-73 22.15391259591436 28.987441864002967 29.398066457801065 19.46057908228161 74-75 22.35732634269858 29.047486264801588 29.23030198618568 19.36488540631415 76-77 22.659386138236844 29.170539112942045 28.88722918927958 19.282845559541535 78-79 22.950013298177197 29.32771028432184 28.509354160673844 19.212922256827124 80-81 23.25161836460208 29.466029302794883 28.126905301162132 19.155447031440907 82-83 23.423911864591407 29.613289224093048 27.82991194122126 19.13288697009429 84-85 23.633482335968647 29.641200210407938 27.61835078970631 19.106966663917106 86-87 23.848882273281095 29.582535966595984 27.502617817850556 19.065963942272365 88-89 24.06259722754751 29.55873683997169 27.381383658846797 18.997282273634 90-91 24.322293635188366 29.497308704601416 27.203877904520095 18.976519755690124 92-93 24.527623906770803 29.45108559329976 27.109370841045887 18.91191965888355 94-95 24.742178766259144 29.433420546116206 26.995606069150142 18.82879461847451 96-97 24.90333213070822 29.378683532041222 26.872874677077757 18.845109660172803 98-99 25.049324592742327 29.360656800959905 26.786042439114034 18.803976167183734 100 25.121259455237777 29.401715522669598 26.67940764742604 18.797617374666583 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 130169.0 1 79233.0 2 22821.0 3 16344.5 4 15025.0 5 15182.5 6 15617.0 7 15287.5 8 14778.5 9 14883.5 10 15746.5 11 21540.0 12 181368.5 13 210585.5 14 79589.0 15 77902.0 16 86991.0 17 96732.0 18 105547.5 19 115191.0 20 128170.5 21 148003.5 22 176343.0 23 207323.5 24 236925.0 25 270315.0 26 310372.0 27 358700.0 28 422425.5 29 489176.5 30 542569.0 31 588219.5 32 637254.0 33 680662.0 34 712485.0 35 749950.5 36 792451.5 37 837984.0 38 894365.0 39 924265.5 40 925794.0 41 943859.5 42 980501.0 43 993563.0 44 984384.5 45 990003.0 46 1001163.5 47 1003290.0 48 986317.0 49 960379.5 50 942084.0 51 924992.0 52 896814.5 53 837589.0 54 761813.5 55 694423.5 56 630715.0 57 565982.0 58 495981.5 59 418447.5 60 337548.5 61 267884.5 62 207207.0 63 152087.0 64 109354.5 65 77409.0 66 54504.0 67 39654.5 68 28645.0 69 20422.5 70 15097.0 71 11430.0 72 8948.5 73 7078.5 74 5815.0 75 4976.0 76 3797.5 77 2305.5 78 1391.0 79 998.0 80 817.0 81 633.5 82 495.5 83 405.0 84 323.5 85 270.5 86 218.0 87 181.5 88 155.5 89 129.0 90 110.0 91 85.5 92 65.5 93 53.0 94 45.0 95 33.5 96 30.5 97 30.5 98 19.0 99 9.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002915188963559666 2 2.527620488635549E-4 3 0.05588400392340622 4 0.016052075183161492 5 0.01122600513019335 6 0.03541701828676131 7 0.023874218055325636 8 0.016877764542782437 9 0.004266623384816807 10-11 0.007230679677823426 12-13 0.012328047663238449 14-15 0.019580633385296718 16-17 0.032270973318572926 18-19 0.040007177094123464 20-21 0.03201821126970938 22-23 0.022088032910023182 24-25 0.01711536086871418 26-27 0.01270213549555651 28-29 0.008491119761489686 30-31 0.008487749600838172 32-33 0.007043635761664395 34-35 0.0060662891727253174 36-37 0.009650455025610525 38-39 0.0133222450554351 40-41 0.013342466019344183 42-43 0.008868577754459261 44-45 0.0119809211161325 46-47 0.015228070903866301 48-49 0.018396021916289522 50-51 0.018422983201501635 52-53 0.015297159197222339 54-55 0.009148301088534929 56-57 0.011136695872928228 58-59 0.017299034624221696 60-61 0.025727806413658374 62-63 0.02854526071832413 64-65 0.016886189944411222 66-67 0.012592605274382303 68-69 0.013692962727101646 70-71 0.45054835209928656 72-73 0.309215609937067 74-75 0.37957613893905184 76-77 0.25542279069792523 78-79 0.2561810768445159 80-81 0.2543696154943271 82-83 0.25395508573419084 84-85 0.24764783007488228 86-87 0.24312675956087615 88-89 0.2366729019132267 90-91 0.24148380624326304 92-93 0.258725548136409 94-95 0.2534866334036304 96-97 0.23213835075661457 98-99 0.23353865250731864 100 0.23434749106368202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.9672176E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.92113004773148 #Duplication Level Percentage of deduplicated Percentage of total 1 71.67660492046596 26.46381251648394 2 13.53635356852452 9.995549409511359 3 5.021155970836643 5.561602577676093 4 2.5031181122958146 3.6967179739562366 5 1.5037356350254145 2.775980946909071 6 1.0106627298783448 2.2388886050540213 7 0.7567244711386213 1.9557385826446905 8 0.566190618666638 1.6723517970877189 9 0.44248015222245995 1.4703180519370918 >10 2.6063165189952606 17.64697198242688 >50 0.1740115992049728 4.473699692510705 >100 0.19606636720353654 14.00388615897626 >500 0.005876166283458078 1.2604268338417672 >1k 6.029078002579904E-4 0.5332017614747462 >5k 4.5573390056032584E-5 0.11038667208127367 >10k+ 5.468806805872551E-5 6.1404664374281195 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1583981 5.338270438945901 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133959 0.4514633507161726 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49599 0.1671565981544461 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 33266 0.11211176423326688 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.011003574527193421 0.0 0.0 0.0 1.6850803257570325E-5 2 0.011067607579572189 3.3701606515140647E-6 0.0 0.0 1.6850803257570325E-5 3 0.011145121274557013 1.3480642606056259E-5 0.0 0.0 1.6850803257570325E-5 4 0.011246226094102434 2.6961285212112518E-5 0.0 3.3701606515140647E-6 2.022096390908439E-5 5 0.011293408343223631 2.6961285212112518E-5 0.0 3.3701606515140647E-6 2.022096390908439E-5 6 0.011377662359511483 3.0331445863626586E-5 0.0 3.3701606515140647E-6 2.6961285212112518E-5 7 0.011441695411890251 3.370160651514065E-5 0.0 3.3701606515140647E-6 2.6961285212112518E-5 8 0.011505728464269017 3.370160651514065E-5 0.0 3.3701606515140647E-6 2.6961285212112518E-5 9 0.011566391355996271 3.707176716665471E-5 0.0 3.3701606515140647E-6 2.6961285212112518E-5 10-11 0.011667496175541692 5.0552409772710975E-5 0.0 6.7403213030281295E-6 2.6961285212112518E-5 12-13 0.011815783244208311 7.582861465906646E-5 0.0 1.1795562280299227E-5 2.6961285212112518E-5 14-15 0.011996086839064314 8.088385563633756E-5 0.0 1.3480642606056259E-5 2.6961285212112518E-5 16-17 0.012130893265124877 8.425401628785163E-5 0.0 1.3480642606056259E-5 2.8646365537869552E-5 18-19 0.012341528305844507 8.930925726512272E-5 0.0 1.3480642606056259E-5 3.0331445863626586E-5 20-21 0.012457798848321742 9.099433759087975E-5 0.0 1.5165722931813291E-5 3.0331445863626586E-5 22-23 0.012649898005458043 9.099433759087975E-5 0.0 2.190604423484142E-5 3.0331445863626586E-5 24-25 0.013072853167223057 9.436449824239381E-5 0.0 2.6961285212112518E-5 3.370160651514065E-5 26-27 0.01426420495753328 1.0110481954542195E-4 0.0 3.0331445863626586E-5 3.538668684089768E-5 28-29 0.017797818400645777 1.0953022117420711E-4 3.3701606515140647E-6 3.0331445863626586E-5 3.8756847492411745E-5 30-31 0.026924213444945864 1.1627054247723525E-4 5.055240977271097E-6 3.0331445863626586E-5 4.549716879543988E-5 32-33 0.0458257594589625 1.196407031287493E-4 6.7403213030281295E-6 3.0331445863626586E-5 4.718224912119691E-5 34-35 0.08076590001353456 1.3143626540904855E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.0552409772710975E-5 36-37 0.1359792419672895 1.348064260605626E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.2237490098468E-5 38-39 0.21770226760585404 1.499721489923759E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.3922570424225036E-5 40-41 0.3294062424002877 1.6176771127267511E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.560765074998207E-5 42-43 0.47320762723974136 1.7019311290146028E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.7292731075739104E-5 44-45 0.6534084321958726 1.803035948560025E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.7292731075739104E-5 46-47 0.8739214137850895 1.8872899648478763E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.7292731075739104E-5 48-49 1.1334288391926497 1.920991571363017E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.7292731075739104E-5 50-51 1.437697053293294 2.022096390908439E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.7292731075739104E-5 52-53 1.785022439877682 2.2074552267417125E-4 6.7403213030281295E-6 3.0331445863626586E-5 5.7292731075739104E-5 54-55 2.1740535645245567 2.3085600462871347E-4 6.7403213030281295E-6 3.0331445863626586E-5 6.066289172725317E-5 56-57 2.6080156709774167 2.3759632593174158E-4 6.7403213030281295E-6 3.0331445863626586E-5 6.066289172725317E-5 58-59 3.086644201625118 2.544471291893119E-4 6.7403213030281295E-6 3.0331445863626586E-5 6.23479720530102E-5 60-61 3.606577421217777 2.712979324468822E-4 6.7403213030281295E-6 3.538668684089768E-5 6.571813270452427E-5 62-63 4.162763458938771 2.898338160302096E-4 6.7403213030281295E-6 3.8756847492411745E-5 6.74032130302813E-5 64-65 4.757128361600444 2.898338160302096E-4 6.7403213030281295E-6 4.044192781816878E-5 6.74032130302813E-5 66-67 5.391163425291088 2.999442979847518E-4 6.7403213030281295E-6 4.212700814392581E-5 6.74032130302813E-5 68-69 6.061196859980879 3.0331445863626585E-4 6.7403213030281295E-6 4.549716879543988E-5 7.077337368179537E-5 70-71 6.762786793931122 3.13424940590808E-4 6.7403213030281295E-6 4.718224912119691E-5 8.088385563633756E-5 72-73 7.494088064185114 3.1848018156807915E-4 6.7403213030281295E-6 4.718224912119691E-5 8.088385563633756E-5 74-75 8.254185672125967 3.269055831968643E-4 6.7403213030281295E-6 4.718224912119691E-5 8.088385563633756E-5 76-77 9.046399225995424 3.3196082417413536E-4 6.7403213030281295E-6 4.718224912119691E-5 8.425401628785163E-5 78-79 9.863157659889858 3.370160651514065E-4 6.7403213030281295E-6 4.718224912119691E-5 8.425401628785163E-5 80-81 10.705497298209607 3.504967077574627E-4 6.7403213030281295E-6 4.718224912119691E-5 9.099433759087975E-5 82-83 11.570737178156397 3.589221093862479E-4 6.7403213030281295E-6 5.2237490098468E-5 9.099433759087975E-5 84-85 12.454201201826251 3.740878323180612E-4 6.7403213030281295E-6 5.560765074998207E-5 9.604957856815084E-5 86-87 13.355377104800134 3.892535552498745E-4 6.7403213030281295E-6 5.7292731075739104E-5 9.773465889390788E-5 88 14.041039659511322 4.0104911753017375E-4 6.7403213030281295E-6 5.7292731075739104E-5 9.773465889390788E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 202885 0.0 86.094635 8 AGTGGTA 204575 0.0 85.90597 5 TGGTATC 204640 0.0 85.199875 7 GTGGTAT 208750 0.0 83.98761 6 GTATCAA 207340 0.0 83.585464 9 AAGCAGT 214975 0.0 83.37625 1 GCAGTGG 214885 0.0 82.90337 3 AGCAGTG 222620 0.0 80.670746 2 CAGTGGT 219365 0.0 80.53995 4 CAACGCA 200085 0.0 45.348583 12-13 ATCAACG 201130 0.0 45.108025 10-11 ACGCAGA 204435 0.0 44.70993 14-15 AGAGTAC 205610 0.0 44.109966 18-19 TCAACGC 200545 0.0 43.198208 12-13 AACGCAG 202325 0.0 42.64417 14-15 TATCAAC 204930 0.0 42.194782 10-11 AGTACTT 214685 0.0 42.051098 20-21 CGCAGAG 205305 0.0 41.92663 16-17 GAGTACT 202690 0.0 41.654644 20-21 CAGAGTA 214675 0.0 39.716732 18-19 >>END_MODULE