FastQCFastQC Report
Thu 26 May 2016
SRR1660698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29672176
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15841475.338829885614051No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1241620.4184458868132893No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550760.18561496804278865No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT339340.11436303154847827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2018400.085.499778
AGTGGTA2032700.085.362035
TGGTATC2038850.084.496737
GTGGTAT2082550.083.141226
AAGCAGT2117650.083.134371
GTATCAA2061650.083.0558249
GCAGTGG2134150.082.321413
AGCAGTG2202000.080.305042
CAGTGGT2185650.079.996644
CAACGCA1983850.045.01265712-13
ATCAACG1991550.044.75864410-11
ACGCAGA2030000.044.3532514-15
AGAGTAC2057600.043.7243318-19
TCAACGC1983500.042.9686312-13
AACGCAG2002900.042.38436514-15
TATCAAC2035600.041.9548710-11
AGTACTT2141550.041.79420520-21
CGCAGAG2039200.041.59794216-17
GAGTACT2022800.041.43531820-21
CAGAGTA2150150.039.3470118-19
Produced by FastQC (version 0.11.5)