FastQCFastQC Report
Thu 26 May 2016
SRR1660697_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660697_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30441996
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19095116.27262088859088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1154370.3792031245257374No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1060170.34825903005834435Illumina Single End PCR Primer 1 (100% over 50bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT472780.15530519089484146No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472750.15529533608768625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2441100.085.514788
AGTGGTA2456050.085.4829565
TGGTATC2453450.084.8733147
AAGCAGT2535900.084.451731
GTGGTAT2489850.084.062666
GCAGTGG2549150.083.628153
GTATCAA2475600.083.5231869
CAGTGGT2579600.081.86154
AGCAGTG2642950.081.241292
CAACGCA2414900.045.01715512-13
ATCAACG2426650.044.8408210-11
ACGCAGA2463200.044.47964514-15
AGAGTAC2479950.043.77951418-19
AGTACTT2519850.042.84059520-21
TCAACGC2416750.042.7165112-13
AACGCAG2432600.042.20479214-15
TATCAAC2457650.041.94669710-11
CGCAGAG2473750.041.41399416-17
GAGTACT2427550.041.3338320-21
GCAGAGT2663100.040.9147316-17
Produced by FastQC (version 0.11.5)