FastQCFastQC Report
Thu 26 May 2016
SRR1660697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30441996
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18025645.921306868314416No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG1086650.3569575398406859TruSeq Adapter, Index 16 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA978900.3215623574748515No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT578330.1899776874026263No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT487730.16021616979385978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2338250.084.681255
GGTATCA2320900.084.653088
TGGTATC2336200.084.056637
AAGCAGT2406250.083.666831
GTGGTAT2376850.083.018566
GCAGTGG2419550.082.8146443
GTATCAA2354500.082.709969
CAGTGGT2464600.080.879534
AGCAGTG2512250.080.410532
CAACGCA2297050.044.61236212-13
ATCAACG2302900.044.3922510-11
ACGCAGA2342750.044.12070514-15
AGAGTAC2378100.043.3555518-19
AGTACTT2406950.042.5479820-21
TCAACGC2297200.042.27075212-13
AACGCAG2311500.041.8232714-15
TATCAAC2342050.041.51006710-11
GAGTACT2320100.041.11794320-21
CGCAGAG2355350.041.05121616-17
GCAGAGT2554650.040.53391616-17
Produced by FastQC (version 0.11.5)