FastQCFastQC Report
Thu 26 May 2016
SRR1660696_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660696_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48276921
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20019584.146822039458565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1187970.24607410236456465No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT511700.10599267505067277No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491860.10188305090956401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2708900.083.3435758
AGTGGTA2740400.082.906555
TGGTATC2748250.082.022417
AAGCAGT2831000.081.900481
GTGGTAT2768900.081.8157356
GTATCAA2773700.080.621879
GCAGTGG2896750.079.6124343
CAGTGGT2906050.078.6734164
AGCAGTG2967900.078.376132
CAACGCA2664750.044.2559412-13
ATCAACG2695250.043.82279610-11
ACGCAGA2724050.043.64251714-15
AGAGTAC2748050.042.8753318-19
TCAACGC2677200.041.6124212-13
AGTACTT2818650.041.51094420-21
AACGCAG2686150.041.2370114-15
TATCAAC2745650.040.64615210-11
CGCAGAG2736700.040.39372616-17
GAGTACT2684850.040.280120-21
GCAGAGT3035900.038.99304616-17
Produced by FastQC (version 0.11.5)