FastQCFastQC Report
Thu 26 May 2016
SRR1660696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48276921
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19617054.063442654099668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090490.2258822595583509No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559870.11597052761504818No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT533750.11056007486475784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2659350.081.983168
AGTGGTA2700050.081.492115
AAGCAGT2758500.080.882131
TGGTATC2700950.080.66357
GTGGTAT2728800.080.291316
GTATCAA2725850.079.24599
GCAGTGG2855950.078.018323
CAGTGGT2865500.077.359664
AGCAGTG2913900.077.0395662
CAACGCA2611300.043.65976312-13
ATCAACG2631050.043.25848810-11
ACGCAGA2674550.043.0351914-15
AGAGTAC2715100.042.20441418-19
AGTACTT2765900.041.1317620-21
TCAACGC2624100.040.9636912-13
AACGCAG2633600.040.57957514-15
TATCAAC2688700.040.08352710-11
GAGTACT2635250.039.85762420-21
CGCAGAG2687450.039.71972316-17
GCAGAGT3009350.038.32954416-17
Produced by FastQC (version 0.11.5)