FastQCFastQC Report
Thu 26 May 2016
SRR1660695_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660695_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55939463
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30152405.390184028044746No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2819240.5039805262342257Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2715280.48539615047788354No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950850.16997839253480143No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT609960.10903930200402531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3926300.086.027878
GTGGTAT3952600.085.6791466
AGTGGTA3969300.085.634925
TGGTATC3967600.084.956877
GTATCAA3999200.083.71929
GCAGTGG4129850.083.520223
AAGCAGT4221000.082.195981
CAGTGGT4219300.080.9813164
AGCAGTG4317100.080.5440442
CAACGCA3848400.045.3915312-13
ATCAACG3868150.045.2384510-11
ACGCAGA3917900.044.91029414-15
AGAGTAC4004700.043.72916818-19
TCAACGC3859850.043.2706812-13
AACGCAG3875300.042.8529914-15
TATCAAC3927700.042.51066210-11
CGCAGAG3938650.042.09861816-17
AGTACTT4167300.041.83080320-21
GAGTACT3933050.041.43752320-21
GCAGAGT4305350.040.68953716-17
Produced by FastQC (version 0.11.5)