##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660695_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55939463 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16080674567791 34.0 31.0 34.0 30.0 34.0 2 32.31030719762183 34.0 31.0 34.0 30.0 34.0 3 32.28542905390422 34.0 31.0 34.0 30.0 34.0 4 35.68549070626581 37.0 35.0 37.0 35.0 37.0 5 35.713683522489305 37.0 35.0 37.0 35.0 37.0 6 35.72245427168294 37.0 35.0 37.0 35.0 37.0 7 35.6661253433913 37.0 35.0 37.0 35.0 37.0 8 35.7187925096814 37.0 35.0 37.0 35.0 37.0 9 37.40050393404742 39.0 38.0 39.0 35.0 39.0 10-11 37.31750173397267 39.0 37.0 39.0 34.5 39.0 12-13 37.36554937969283 39.0 38.0 39.0 35.0 39.0 14-15 38.775608115866255 40.0 39.0 41.0 35.0 41.0 16-17 38.71287301238483 40.0 38.5 41.0 35.0 41.0 18-19 38.585454958693475 40.0 38.0 41.0 34.0 41.0 20-21 38.5183307712482 40.0 38.0 41.0 34.0 41.0 22-23 38.35939873073147 40.0 38.0 41.0 34.0 41.0 24-25 38.354451230609776 40.0 38.0 41.0 34.0 41.0 26-27 38.334505776360416 40.0 38.0 41.0 34.0 41.0 28-29 38.23939596095157 40.0 38.0 41.0 33.5 41.0 30-31 38.110002155365706 40.0 38.0 41.0 33.0 41.0 32-33 37.987871746284014 40.0 38.0 41.0 33.0 41.0 34-35 37.8870861702766 40.0 38.0 41.0 33.0 41.0 36-37 37.70875378263821 40.0 37.5 41.0 32.5 41.0 38-39 37.40006460197875 40.0 36.0 41.0 32.5 41.0 40-41 37.4485083455306 40.0 36.0 41.0 32.5 41.0 42-43 37.31310682228036 40.0 35.5 41.0 32.0 41.0 44-45 37.18762153651707 40.0 35.0 41.0 31.5 41.0 46-47 37.27193080133787 40.0 36.0 41.0 32.0 41.0 48-49 37.24239504229778 40.0 35.5 41.0 31.5 41.0 50-51 35.86466550456518 38.5 34.0 40.0 29.0 40.5 52-53 35.978615391785226 38.5 35.0 39.5 29.5 40.5 54-55 36.42074251767487 39.5 35.0 41.0 28.5 41.0 56-57 36.08141931930952 40.0 35.0 41.0 26.5 41.0 58-59 35.88521176007714 39.0 35.0 41.0 26.0 41.0 60-61 35.7191507362164 39.0 35.0 41.0 26.0 41.0 62-63 35.50482738813564 39.0 35.0 41.0 26.0 41.0 64-65 35.21404907837603 38.0 35.0 41.0 25.5 41.0 66-67 34.87085654540516 37.5 34.5 40.0 25.0 41.0 68-69 34.595618141704364 37.0 34.0 40.0 25.0 41.0 70-71 34.27736078732111 36.5 34.0 39.5 24.0 41.0 72-73 33.96614991638371 36.0 34.0 39.0 24.0 41.0 74-75 33.58945740147702 35.5 34.0 39.0 23.5 40.5 76-77 33.19889949604986 35.0 33.0 37.5 23.0 40.0 78-79 32.81039839442148 35.0 33.0 37.0 21.5 39.0 80-81 32.41207714131971 35.0 33.0 36.5 20.0 39.0 82-83 32.110387285984494 35.0 33.0 36.0 20.0 38.5 84-85 31.80835323714137 35.0 32.0 36.0 19.0 37.0 86-87 31.540026671332186 35.0 32.0 35.0 19.0 37.0 88-89 31.362951044059898 35.0 32.0 35.0 19.0 36.5 90-91 31.165856427688624 35.0 32.0 35.0 18.0 36.0 92-93 31.007909174959366 35.0 32.0 35.0 18.0 36.0 94-95 30.854728011958212 35.0 31.0 35.0 17.5 36.0 96-97 30.714179755354465 35.0 31.0 35.0 17.0 35.0 98-99 30.585824652982456 35.0 31.0 35.0 16.0 35.0 100 30.477756391762288 35.0 31.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3471934092711777 1101 2 0.32166797587276363 1101 3 0.32155823740214373 1101 4 0.26560101372120926 1101 5 0.26772495943692576 1101 6 0.20383871394513875 1101 7 0.23554469771237052 1101 8 0.24879115683123132 1101 9 0.3320148027455687 1101 10-11 0.3149593629235454 1101 12-13 0.31767136439226107 1101 14-15 0.38736979499680047 1101 16-17 0.3829276411095677 1101 18-19 0.42288212927219604 1101 20-21 0.36925355183765873 1101 22-23 0.3612251876484791 1101 24-25 0.36770872918148 1101 26-27 0.3833927786507232 1101 28-29 0.3500351806458184 1101 30-31 0.3537214400941977 1101 32-33 0.34574632831195373 1101 34-35 0.32780677091983534 1101 36-37 0.3238801155318498 1101 38-39 0.33851518487784915 1101 40-41 0.2990763008873216 1101 42-43 0.26974497577595 1101 44-45 0.26392939836688356 1101 46-47 0.2963436864406148 1101 48-49 0.3313426748630448 1101 50-51 0.2572965865201553 1101 52-53 0.34839460517163445 1101 54-55 0.3809134379762469 1101 56-57 0.4332492228190148 1101 58-59 0.40308349462289783 1101 60-61 0.3672032391750477 1101 62-63 0.3363851906354256 1101 64-65 0.3139615817059891 1101 66-67 0.31415038934392925 1101 68-69 0.2944189994618327 1101 70-71 0.2755263138290047 1101 72-73 0.2708703141081372 1101 74-75 0.2514961921143808 1101 76-77 0.23541170469061967 1101 78-79 0.23204972116168676 1101 80-81 0.241546809870691 1101 82-83 0.19659478182916956 1101 84-85 0.1874772993672167 1101 86-87 0.22067788112394027 1101 88-89 0.17535278049957626 1101 90-91 0.1337622597581678 1101 92-93 0.11416114022645729 1101 94-95 0.1402036755439262 1101 96-97 0.10336868989148584 1101 98-99 0.051808768963514495 1101 100 0.03303590494606823 1102 1 0.3445274416720707 1102 2 0.32550099248322084 1102 3 0.3256626371746023 1102 4 0.30420454363838445 1102 5 0.28321810518031754 1102 6 0.20507501766338976 1102 7 0.24133971718508462 1102 8 0.21799474745738934 1102 9 0.3139251755261796 1102 10-11 0.32799067484658195 1102 12-13 0.30217748866680694 1102 14-15 0.3605585350009406 1102 16-17 0.3914978740783326 1102 18-19 0.407793287232721 1102 20-21 0.39079529563037596 1102 22-23 0.324336549334582 1102 24-25 0.32841801986052843 1102 26-27 0.3434600399110721 1102 28-29 0.34079462209534483 1102 30-31 0.33601943589344074 1102 32-33 0.31425796827638663 1102 34-35 0.33110151255270637 1102 36-37 0.3110162800374212 1102 38-39 0.327088882266537 1102 40-41 0.28640388446687126 1102 42-43 0.2681211872932465 1102 44-45 0.2577620493397177 1102 46-47 0.2990438470623573 1102 48-49 0.316051052659553 1102 50-51 0.26785820795709014 1102 52-53 0.33792294551387414 1102 54-55 0.4540902550253918 1102 56-57 0.4194398368163874 1102 58-59 0.4348468575274538 1102 60-61 0.3813009774444538 1102 62-63 0.3579247483105803 1102 64-65 0.3504431197530451 1102 66-67 0.38568899523480127 1102 68-69 0.3860143265911873 1102 70-71 0.3907023339317348 1102 72-73 0.32369421289742206 1102 74-75 0.3172876276645198 1102 76-77 0.30050188996511196 1102 78-79 0.30348286719303275 1102 80-81 0.3463710949651144 1102 82-83 0.30758512699645024 1102 84-85 0.26089171238490394 1102 86-87 0.2911942637187721 1102 88-89 0.24060731978530114 1102 90-91 0.23197215881939925 1102 92-93 0.19987218588273947 1102 94-95 0.19692698059865776 1102 96-97 0.18598617500985526 1102 98-99 0.14808125526281302 1102 100 0.11521752527196938 1103 1 0.24496928156063547 1103 2 0.22524240250146477 1103 3 0.2489548553895844 1103 4 0.21772720307484406 1103 5 0.20400430168425032 1103 6 0.12175094549545662 1103 7 0.1525991584901405 1103 8 0.16579225875714343 1103 9 0.22807365564135296 1103 10-11 0.24169163421904472 1103 12-13 0.23981571100539867 1103 14-15 0.28095933947893315 1103 16-17 0.2820583193004964 1103 18-19 0.2963428749253154 1103 20-21 0.2607231188094872 1103 22-23 0.269501595459154 1103 24-25 0.27878123643056085 1103 26-27 0.27968752702705757 1103 28-29 0.27861238793718 1103 30-31 0.22001563610084673 1103 32-33 0.2495342862800456 1103 34-35 0.25972817091704314 1103 36-37 0.22531578596657198 1103 38-39 0.2091873770519186 1103 40-41 0.20123324247533247 1103 42-43 0.14843293264297586 1103 44-45 0.2054621309191802 1103 46-47 0.2794997955987739 1103 48-49 0.240991603323657 1103 50-51 0.19228149922436444 1103 52-53 0.25141110715968296 1103 54-55 0.329420915359961 1103 56-57 0.3147591410611028 1103 58-59 0.30245869431542616 1103 60-61 0.28056676113138224 1103 62-63 0.22935909085528294 1103 64-65 0.23633891152000785 1103 66-67 0.2651990538258957 1103 68-69 0.28112339370342454 1103 70-71 0.29483870758672737 1103 72-73 0.2245114942557862 1103 74-75 0.23891183592610332 1103 76-77 0.21347565996654794 1103 78-79 0.2231658738361162 1103 80-81 0.2777931940271756 1103 82-83 0.20007965753259072 1103 84-85 0.2061844645112103 1103 86-87 0.20069643794011327 1103 88-89 0.15335787572121262 1103 90-91 0.14861498822713415 1103 92-93 0.1483949588426441 1103 94-95 0.1649853640218346 1103 96-97 0.14548419545018376 1103 98-99 0.12890691702936508 1103 100 0.10890059030500154 1104 1 0.21362148758434785 1104 2 0.18835390814681574 1104 3 0.19530443065544034 1104 4 0.22616412518803486 1104 5 0.18298954026972325 1104 6 0.14054782553629508 1104 7 0.1510013735278548 1104 8 0.17155501191448508 1104 9 0.23104186247901737 1104 10-11 0.22000981637285122 1104 12-13 0.23376284894067823 1104 14-15 0.23166747705756308 1104 16-17 0.2536995813319507 1104 18-19 0.2971106596697055 1104 20-21 0.24234571244542025 1104 22-23 0.23846534423366705 1104 24-25 0.25068614700686 1104 26-27 0.25960566871766844 1104 28-29 0.22601546705597286 1104 30-31 0.2552675446862267 1104 32-33 0.21837657648192987 1104 34-35 0.2280885797368697 1104 36-37 0.20808133847603472 1104 38-39 0.21381673087777386 1104 40-41 0.1837581941185178 1104 42-43 0.16042697276790108 1104 44-45 0.14345362379523863 1104 46-47 0.16200471934987348 1104 48-49 0.19461649838581252 1104 50-51 0.1959409893964832 1104 52-53 0.22255993633586257 1104 54-55 0.22637755531816595 1104 56-57 0.29112207224148534 1104 58-59 0.2765788949354402 1104 60-61 0.24106136731508343 1104 62-63 0.2206719722428474 1104 64-65 0.24502991237859817 1104 66-67 0.2487829411994511 1104 68-69 0.24767625697852935 1104 70-71 0.2586494198590046 1104 72-73 0.17810200376341356 1104 74-75 0.212629182560363 1104 76-77 0.18119795045570442 1104 78-79 0.16243522936564858 1104 80-81 0.2299211999777313 1104 82-83 0.19919541300165378 1104 84-85 0.21980752118412283 1104 86-87 0.21181846380619263 1104 88-89 0.1401249606629058 1104 90-91 0.14182560681522105 1104 92-93 0.12007686649830873 1104 94-95 0.10766438667054956 1104 96-97 0.14432957920086764 1104 98-99 0.14012637477506118 1104 100 0.12038814338010084 1105 1 0.1308671904594263 1105 2 0.12421295847931191 1105 3 0.14381024118599584 1105 4 0.1450510628626418 1105 5 0.12501689042524333 1105 6 0.0522505831851916 1105 7 0.07355925501625649 1105 8 0.08361796165877422 1105 9 0.1108668601615932 1105 10-11 0.09890159201306403 1105 12-13 0.1196379639195797 1105 14-15 0.11286184422058199 1105 16-17 0.13974326586558306 1105 18-19 0.1994171589720395 1105 20-21 0.15993059873261473 1105 22-23 0.1333209780600555 1105 24-25 0.13334597296423567 1105 26-27 0.1513460213195046 1105 28-29 0.1492656062067823 1105 30-31 0.14736469225426418 1105 32-33 0.16458520679571365 1105 34-35 0.15652623863195458 1105 36-37 0.11607283368640253 1105 38-39 0.15053769331131406 1105 40-41 0.11696646295932567 1105 42-43 0.123486815509537 1105 44-45 0.08947186211948122 1105 46-47 0.15700304330522385 1105 48-49 0.13943551466844895 1105 50-51 0.08945178679216781 1105 52-53 0.1778919375257786 1105 54-55 0.2133816772759829 1105 56-57 0.1879598286920583 1105 58-59 0.17727849046774935 1105 60-61 0.1603528773791112 1105 62-63 0.19915557464303646 1105 64-65 0.16716098685066783 1105 66-67 0.19861045861975413 1105 68-69 0.20623137919421453 1105 70-71 0.2550861769132311 1105 72-73 0.20466168329115675 1105 74-75 0.19027793683248717 1105 76-77 0.15399678377755777 1105 78-79 0.16827410583322688 1105 80-81 0.17965670960101932 1105 82-83 0.1817962494855081 1105 84-85 0.17500577365919412 1105 86-87 0.16622287400103986 1105 88-89 0.13272908898442637 1105 90-91 0.15902523568723836 1105 92-93 0.17364537848535377 1105 94-95 0.11039572035056011 1105 96-97 0.11542092005901239 1105 98-99 0.11977493446417142 1105 100 0.09142695118860544 1106 1 0.12711714511736005 1106 2 0.10526150167772386 1106 3 0.12483235188962993 1106 4 0.11406361906272622 1106 5 0.09693404718228038 1106 6 0.05322271849970406 1106 7 0.08780210825670309 1106 8 0.10905363163958981 1106 9 0.11806038348748871 1106 10-11 0.08027537357835257 1106 12-13 0.06080611151931237 1106 14-15 0.11107206927301405 1106 16-17 0.11063120908813318 1106 18-19 0.16402922863058933 1106 20-21 0.13837840712655947 1106 22-23 0.138964859385716 1106 24-25 0.11147731445159081 1106 26-27 0.1293388395311652 1106 28-29 0.0981955273900823 1106 30-31 0.10549848979720622 1106 32-33 0.11414781138400798 1106 34-35 0.053892138579243465 1106 36-37 0.0799422193417314 1106 38-39 0.06278177278649366 1106 40-41 0.07652570721316465 1106 42-43 0.10776686757580478 1106 44-45 0.11411482334700906 1106 46-47 0.09613993420911271 1106 48-49 0.12021857513573053 1106 50-51 0.021114209225160607 1106 52-53 0.12306485432151248 1106 54-55 0.14626685275030127 1106 56-57 0.19352072080596372 1106 58-59 0.1943961755525052 1106 60-61 0.14022955445119578 1106 62-63 0.1491896950196292 1106 64-65 0.13414336055768672 1106 66-67 0.1376731547943919 1106 68-69 0.13298617160027248 1106 70-71 0.18743405148126868 1106 72-73 0.12336845702338195 1106 74-75 0.0853458413079835 1106 76-77 0.0677770681258707 1106 78-79 0.11873686497552427 1106 80-81 0.13457599216712168 1106 82-83 0.126624273123511 1106 84-85 0.14355317170307558 1106 86-87 0.15208272932547118 1106 88-89 0.09520063355514807 1106 90-91 0.14863353714459393 1106 92-93 0.10740588406990881 1106 94-95 0.08822967266558024 1106 96-97 0.08099074032433506 1106 98-99 0.09657117404702475 1106 100 0.10697688080225021 1107 1 0.04931094721819562 1107 2 0.05465211268199255 1107 3 0.03490873170442654 1107 4 0.06823246458160526 1107 5 0.07672016711251928 1107 6 0.02968382555906146 1107 7 0.0038688123475409952 1107 8 0.005867923466404079 1107 9 0.035676887424898496 1107 10-11 0.03087392886073559 1107 12-13 0.050812786448695135 1107 14-15 0.012326616730874207 1107 16-17 0.0190970774331376 1107 18-19 0.06923272246353918 1107 20-21 0.03063468127379565 1107 22-23 0.020717513296155232 1107 24-25 0.0018207318878182832 1107 26-27 -0.009746107046773034 1107 28-29 0.0559261259536612 1107 30-31 0.005130460594095609 1107 32-33 -0.03493083088299187 1107 34-35 -0.020462810606076687 1107 36-37 -0.02180101304129778 1107 38-39 0.0404535961577821 1107 40-41 0.00729323191592357 1107 42-43 -0.026567589800507108 1107 44-45 0.012096837345168865 1107 46-47 0.01163889967680376 1107 48-49 -0.024144458648230227 1107 50-51 -0.08648755439555345 1107 52-53 0.02440403630265564 1107 54-55 0.06556029514933925 1107 56-57 0.13140312315256608 1107 58-59 0.12609353677763124 1107 60-61 0.10009062774908273 1107 62-63 0.07322458497115747 1107 64-65 0.10537299892781249 1107 66-67 0.14935178069538324 1107 68-69 0.11589267083109434 1107 70-71 0.14664230929201238 1107 72-73 0.07279947632416395 1107 74-75 0.0765894028539833 1107 76-77 0.0633604645152488 1107 78-79 0.10462011197099486 1107 80-81 0.14059367784987842 1107 82-83 0.06324439868861731 1107 84-85 0.09748583993989257 1107 86-87 0.15306081700690655 1107 88-89 0.06657504717529505 1107 90-91 0.09290242686437011 1107 92-93 0.08483167571096217 1107 94-95 0.07656626805595934 1107 96-97 0.08164398390380256 1107 98-99 0.0742550121797727 1107 100 0.06417379201238305 1108 1 -0.024166125498275903 1108 2 -0.032391172409518276 1108 3 0.02360365816345933 1108 4 0.06277085188316534 1108 5 0.044570435253405094 1108 6 0.011698620355232947 1108 7 -0.01759319655388225 1108 8 0.01187388685296753 1108 9 0.011032794969722204 1108 10-11 -0.025217940029698127 1108 12-13 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2316 48-49 -0.9545154290757338 2316 50-51 -0.9246073118956417 2316 52-53 -1.098792125188936 2316 54-55 -1.0531183143421359 2316 56-57 -1.4357749369211916 2316 58-59 -1.3233276040092434 2316 60-61 -1.1823741408826578 2316 62-63 -1.1993976966191369 2316 64-65 -1.0230079368511937 2316 66-67 -0.7915849504849319 2316 68-69 -0.8413667334707071 2316 70-71 -0.8100530591835948 2316 72-73 -0.9154627119813341 2316 74-75 -0.8294415636311641 2316 76-77 -0.9194467685067949 2316 78-79 -0.8003258305692142 2316 80-81 -0.7799596441975112 2316 82-83 -0.8854548433003728 2316 84-85 -1.094258374934718 2316 86-87 -1.0657893663403044 2316 88-89 -0.8340965600234647 2316 90-91 -0.882359455329528 2316 92-93 -0.9822769248283016 2316 94-95 -1.0409584842702166 2316 96-97 -0.8601725971016521 2316 98-99 -1.0108485719869726 2316 100 -1.1397658310839596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 122.0 3 262.0 4 185.0 5 114.0 6 1030.0 7 13624.0 8 35133.0 9 47289.0 10 49481.0 11 49886.0 12 54374.0 13 64251.0 14 80256.0 15 102489.0 16 128558.0 17 158586.0 18 177522.0 19 200656.0 20 230714.0 21 281121.0 22 368044.0 23 528391.0 24 770035.0 25 1005017.0 26 1054488.0 27 908278.0 28 721730.0 29 723726.0 30 819062.0 31 991775.0 32 1236445.0 33 1598101.0 34 2222409.0 35 3571646.0 36 5996361.0 37 1.1350425E7 38 1.526992E7 39 5118839.0 40 9118.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.934246128459307 36.48721781241236 26.999223982793186 19.57931207633515 2 20.37614381362175 33.49896732896787 27.95475360998675 18.170135247423634 3 25.207316002675146 27.4347488011931 26.947426697888854 20.4105084982429 4 18.849756546034957 27.315757675544628 27.373010984395712 26.461474794024696 5 19.885119283492138 33.37572122728598 26.650784288381956 20.088375200839927 6 26.864081249841814 26.761844620105457 26.941144179887672 19.432929950165054 7 20.314978392051604 26.989486020250396 33.229431174531875 19.466104413166118 8 25.882436246060013 27.37006319627322 27.052037126525313 19.69546343114145 9 25.67790535328666 27.124900773572385 27.525521413502418 19.671672459638533 10-11 19.566759236835836 30.703539125773798 30.206682279249215 19.52301935814115 12-13 19.633502276718716 27.2873400146714 29.907727005227656 23.171430703382224 14-15 19.61328152363187 33.770408362376564 26.94812603037853 19.668184083613035 16-17 23.04812143630894 27.10648337745063 26.661603687547192 23.18379149869324 18-19 19.953930710015555 30.499996632649513 26.87508363036301 22.67098902697192 20-21 22.835276748153177 30.793979917989233 26.87328346137672 19.497459872480874 22-23 23.200243506827327 27.369483434007734 29.897549392922713 19.532723666242223 24-25 19.73866378614107 30.601202080892946 26.698150154870447 22.96198397809553 26-27 19.48845750306888 27.736974083573873 33.24542676111592 19.52914165224133 28-29 19.777248952159443 27.511063038510958 33.22128285933205 19.490405149997546 30-31 19.798725746013123 27.207102592826725 33.462433818428416 19.531737842731733 32-33 19.837766319973447 27.25256458190945 33.38883138712028 19.520837710996826 34-35 19.83986420818663 27.618776383232014 33.04539405325686 19.4959653553245 36-37 19.54765758865486 27.60357077623881 33.31622921227799 19.532542422828335 38-39 19.59590350820043 27.322936526536367 33.27068046917703 19.810479496086174 40-41 19.917590060487782 27.401360313611335 32.93396809819706 19.747081527703816 42-43 19.94923077444295 27.45590092509162 33.138421160987505 19.456447139477923 44-45 20.262946130717687 27.518476952866 32.77784023848521 19.440736677931106 46-47 19.71690727414155 27.602779196735653 32.985286744852154 19.69502678427064 48-49 20.08504499553111 27.616597321175956 32.57198229798844 19.726375385304497 50-51 20.15857836330547 27.66626643835806 32.46675010889112 19.708405089445353 52-53 19.97620143759935 28.11256534455152 32.49302142008267 19.418211797766457 54-55 20.292425559151088 28.08481771157415 31.752065749160153 19.87069098011461 56-57 20.72755212690594 28.89691998511391 30.678354462111233 19.697173425868915 58-59 20.921263888866665 29.36295031728198 30.07254431457211 19.64324147927924 60-61 21.04166366425409 29.927954078053475 29.43185598661518 19.598526271077247 62-63 21.13959919761883 30.069974867004802 29.241056035871065 19.5493698995053 64-65 21.277448392768864 30.10264884663798 29.165168749894686 19.45473401069847 66-67 21.428158217978496 29.863223335294425 29.325594981753994 19.383023464973085 68-69 21.540387865803307 29.549212497551895 29.60798491897861 19.302414717666192 70-71 21.705739610068797 29.348602786997287 29.748978711876738 19.196678891057182 72-73 21.860516696468576 29.2449772533391 29.728709653023582 19.165796397168748 74-75 22.083780929353107 29.260582056585825 29.54498949031451 19.110647523746554 76-77 22.34840255470258 29.37932816235365 29.239521610101825 19.03274767284194 78-79 22.611412603814955 29.483799478635962 28.909274507856203 18.99551340969288 80-81 22.890969783155146 29.588300211959666 28.566885448486012 18.953844556399176 82-83 23.07838680221716 29.705449450782623 28.276238079889847 18.939925667110373 84-85 23.244681760642642 29.72831134655849 28.07907059867475 18.94793629412412 86-87 23.445760772606366 29.685766827990427 27.9609852849269 18.90748711447631 88-89 23.625319769391428 29.671413863989294 27.85149355144632 18.851772815172954 90-91 23.83868984441139 29.62415121248107 27.674513302471084 18.862645640636455 92-93 23.99400461412491 29.600884586229746 27.59147032695608 18.813640472689258 94-95 24.151391360922414 29.617078529502912 27.478532298876484 18.752997810698194 96-97 24.24624573366332 29.63823974871154 27.36948869854633 18.74602581907881 98-99 24.356383764347584 29.65359381127472 27.276511269089376 18.713511155288316 100 24.416467739700924 29.71120429819198 27.180140287425647 18.692187674681453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263339.0 1 156295.0 2 39662.0 3 29134.5 4 27985.5 5 27622.0 6 27989.5 7 28427.0 8 28104.0 9 27940.5 10 29554.0 11 43352.0 12 371461.0 13 430827.5 14 161970.5 15 158057.5 16 176105.5 17 194875.5 18 212508.5 19 228478.0 20 249851.0 21 286033.5 22 339670.5 23 402295.5 24 465478.5 25 538446.5 26 627550.5 27 733440.0 28 856510.5 29 974441.5 30 1069597.0 31 1153538.0 32 1249611.0 33 1340820.5 34 1409030.0 35 1483952.5 36 1580411.5 37 1675945.0 38 1747877.5 39 1779720.5 40 1785983.0 41 1798379.5 42 1832738.5 43 1842553.5 44 1844408.0 45 1865401.0 46 1865577.0 47 1853494.5 48 1813438.0 49 1752991.0 50 1702972.0 51 1647238.0 52 1569054.0 53 1457535.0 54 1324276.0 55 1203848.0 56 1098568.0 57 994071.5 58 877247.5 59 745846.5 60 611311.0 61 494118.0 62 388351.0 63 288452.0 64 207270.5 65 146611.0 66 104779.5 67 77792.0 68 57476.0 69 42156.0 70 31857.0 71 24831.0 72 19939.0 73 15744.5 74 12469.0 75 10356.0 76 8141.5 77 5606.0 78 3925.0 79 3013.5 80 2314.5 81 1900.5 82 1539.0 83 1168.0 84 975.5 85 854.0 86 747.0 87 659.5 88 569.0 89 468.0 90 393.5 91 349.0 92 291.5 93 258.5 94 242.0 95 201.5 96 154.0 97 120.5 98 89.5 99 59.0 100 36.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0045745880685340145 2 1.0904645259108047E-4 3 0.08964154697015951 4 0.02558301283657299 5 0.017826413528495975 6 0.05704023293895403 7 0.03781051670088431 8 0.025899426313763508 9 0.005370090878419766 10-11 0.007574259338170622 12-13 0.014661384933208958 14-15 0.026690460006739785 16-17 0.04954909917529956 18-19 0.06252562703363813 20-21 0.04530075664115689 22-23 0.026730682058925023 24-25 0.019272619760400633 26-27 0.017552009750254484 28-29 0.012391073543197939 30-31 0.010779509985642872 32-33 0.007988099563987591 34-35 0.005807170512165982 36-37 0.009965236884737345 38-39 0.014292235876486693 40-41 0.015786708571013632 42-43 0.009620221059326221 44-45 0.003707579388096736 46-47 0.002684151615827989 48-49 0.0019208264476904258 50-51 9.975068941938182E-4 52-53 0.0014122409433926816 54-55 2.368631962019371E-4 56-57 0.002396340486858088 58-59 0.010472928565653196 60-61 0.021839680513200496 62-63 0.027117707583285165 64-65 0.008644165926297862 66-67 0.002132662589199328 68-69 0.001450675348814128 70-71 0.002072776422612423 72-73 6.676860662748943E-4 74-75 6.229948971801892E-4 76-77 0.0013228586052032713 78-79 0.0031686038888145925 80-81 0.007251589097306851 82-83 0.008463613603155253 84-85 0.007253376744070639 86-87 0.004408336919501712 88-89 0.0023230469695427716 90-91 0.0025652731060360734 92-93 0.0025840433970558494 94-95 0.003157878008231863 96-97 0.0032821194583151432 98-99 0.007385662604590966 100 0.013039095495071163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.5939463E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.14555615988943 #Duplication Level Percentage of deduplicated Percentage of total 1 65.74343993024571 19.16129120631294 2 15.294225437492662 8.915174128209037 3 6.328431605232152 5.533369762629388 4 3.169210305552588 3.6947358777193324 5 1.9305338699048338 2.8133241661940005 6 1.2044565696583198 2.1062733955874604 7 0.8900948274441856 1.8159616146631121 8 0.6833028513773264 1.593219330322436 9 0.5369642025377067 1.4085108288821677 >10 3.6102173748010573 20.357393183216647 >50 0.3365324416048664 6.987022296845136 >100 0.2507295770165264 14.098080058430753 >500 0.020776237511555683 3.857118250871963 >1k 8.76747314353982E-4 0.520843857957781 >5k 1.3460277350724065E-4 0.2918051241087148 >10k+ 7.34195326067513E-5 6.845876918049433 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3015240 5.390184028044746 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 281924 0.5039805262342257 Illumina Single End PCR Primer 1 (100% over 50bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 271528 0.48539615047788354 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95085 0.16997839253480143 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 60996 0.10903930200402531 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.5857671533242998 3.5752935275764087E-6 0.0 0.0 3.217764174818768E-5 2 0.5857850297919378 1.251352734651743E-5 0.0 0.0 3.217764174818768E-5 3 0.5858011186128118 1.4301174110305635E-5 1.7876467637882043E-6 0.0 3.217764174818768E-5 4 0.5858029062595757 1.4301174110305635E-5 1.7876467637882043E-6 0.0 3.217764174818768E-5 5 0.5858118444933946 1.7876467637882044E-5 1.7876467637882043E-6 0.0 3.217764174818768E-5 6 0.5859316168265684 2.1451761165458454E-5 1.7876467637882043E-6 0.0 3.217764174818768E-5 7 0.5859351921200959 2.6814701456823065E-5 1.7876467637882043E-6 0.0 3.217764174818768E-5 8 0.5859369797668598 2.860234822061127E-5 1.7876467637882043E-6 0.0 3.217764174818768E-5 9 0.5859405550603873 3.217764174818768E-5 1.7876467637882043E-6 1.7876467637882043E-6 3.217764174818768E-5 10-11 0.5859503871175882 3.217764174818768E-5 1.7876467637882043E-6 1.7876467637882043E-6 3.3071465130081785E-5 12-13 0.5859700512319899 3.3965288511975884E-5 1.7876467637882043E-6 1.7876467637882043E-6 3.3965288511975884E-5 14-15 0.585994184463301 4.469116909470511E-5 1.7876467637882043E-6 1.7876467637882043E-6 4.0222052185234604E-5 16-17 0.5860138485777027 5.005410938606972E-5 1.7876467637882043E-6 1.7876467637882043E-6 4.20096989490228E-5 18-19 0.5860361941622501 5.809851982311664E-5 1.7876467637882043E-6 3.5752935275764087E-6 4.647881585849332E-5 20-21 0.5860889297417817 7.061204716963406E-5 1.7876467637882043E-6 3.5752935275764087E-6 4.647881585849332E-5 22-23 0.586230153836121 8.04441043704692E-5 1.7876467637882043E-6 5.3629402913646135E-6 4.647881585849332E-5 24-25 0.5868415290293366 8.133792775236331E-5 1.7876467637882043E-6 5.3629402913646135E-6 4.826646262228152E-5 26-27 0.5889518460339885 8.49132212799397E-5 1.7876467637882043E-6 5.3629402913646135E-6 5.005410938606972E-5 28-29 0.5953337449807125 9.027616157130432E-5 1.7876467637882043E-6 7.150587055152817E-6 5.094793276796382E-5 30-31 0.6098235158245977 9.921439539024534E-5 1.7876467637882043E-6 8.938233818941022E-6 5.1841756149857925E-5 32-33 0.6387530034029822 1.0547115906350406E-4 1.7876467637882043E-6 1.0725880582729227E-5 5.273557953175203E-5 34-35 0.6873278350920172 1.0904645259108046E-4 1.7876467637882043E-6 1.6982644255987942E-5 5.720469644122254E-5 36-37 0.761909351900643 1.1440939288244508E-4 1.7876467637882043E-6 1.966411440167025E-5 5.720469644122254E-5 38-39 0.8656545380137095 1.1619703964623329E-4 1.7876467637882043E-6 2.055793778356435E-5 5.8992343205010744E-5 40-41 1.002081482262352 1.197723331738097E-4 1.7876467637882043E-6 2.1451761165458454E-5 5.8992343205010744E-5 42-43 1.1738859559663632 1.215599799375979E-4 1.7876467637882043E-6 2.1451761165458454E-5 5.8992343205010744E-5 44-45 1.3832855349362219 1.251352734651743E-4 1.7876467637882043E-6 2.1451761165458454E-5 5.8992343205010744E-5 46-47 1.6319212789010864 1.3764880081169173E-4 1.7876467637882043E-6 2.1451761165458454E-5 5.8992343205010744E-5 48-49 1.9216809428435164 1.3943644757547993E-4 1.7876467637882043E-6 2.1451761165458454E-5 5.8992343205010744E-5 50-51 2.2514088131307233 1.4479938786684455E-4 1.7876467637882043E-6 2.323940792924666E-5 5.8992343205010744E-5 52-53 2.6195764517796674 1.4479938786684455E-4 1.7876467637882043E-6 2.7708524838717167E-5 5.8992343205010744E-5 54-55 3.025549244189205 1.5105615154010327E-4 1.7876467637882043E-6 2.860234822061127E-5 5.988616658690485E-5 56-57 3.469078707459169 1.599943853590443E-4 1.7876467637882043E-6 2.9496171602505372E-5 6.524910687826947E-5 58-59 3.949867198403388 1.653573256504089E-4 1.7876467637882043E-6 3.0389994984399474E-5 6.793057702395177E-5 60-61 4.465678370920364 1.716140893236676E-4 1.7876467637882043E-6 3.0389994984399474E-5 6.793057702395177E-5 62-63 5.010103511361917 1.7876467637882045E-4 1.7876467637882043E-6 3.3071465130081785E-5 6.793057702395177E-5 64-65 5.5832731179418005 1.8233996990639685E-4 1.7876467637882043E-6 3.6646758657658194E-5 6.971822378773997E-5 66-67 6.185306069169809 1.8233996990639685E-4 1.7876467637882043E-6 3.843440542144639E-5 7.150587055152818E-5 68-69 6.816152310936557 1.8859673357965557E-4 1.7876467637882043E-6 4.469116909470511E-5 7.150587055152818E-5 70-71 7.472544918781219 1.9753496739859658E-4 1.7876467637882043E-6 4.469116909470511E-5 7.150587055152818E-5 72-73 8.15156824083206 2.055793778356435E-4 1.7876467637882043E-6 4.469116909470511E-5 7.239969393342228E-5 74-75 8.852339179587762 2.073670245994317E-4 1.7876467637882043E-6 4.469116909470511E-5 7.597498746099869E-5 76-77 9.575492349649478 2.1183614150890222E-4 1.7876467637882043E-6 4.469116909470511E-5 7.8656457606681E-5 78-79 10.316670362030468 2.1719908180026684E-4 3.5752935275764087E-6 4.469116909470511E-5 8.04441043704692E-5 80-81 11.076897538326387 2.2434966885541966E-4 6.256763673258715E-6 4.647881585849332E-5 8.04441043704692E-5 82-83 11.855609875983257 2.3239407929246658E-4 7.150587055152817E-6 4.737263924038742E-5 8.04441043704692E-5 84-85 12.650290368357666 2.3596937282004298E-4 7.150587055152817E-6 4.826646262228152E-5 8.22317511342574E-5 86-87 13.463605290597801 2.5027054693034864E-4 7.150587055152817E-6 4.916028600417562E-5 8.22317511342574E-5 88 14.083749785013131 2.5384584045792504E-4 7.150587055152817E-6 5.005410938606972E-5 8.580704466183382E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 392630 0.0 86.02787 8 GTGGTAT 395260 0.0 85.679146 6 AGTGGTA 396930 0.0 85.63492 5 TGGTATC 396760 0.0 84.95687 7 GTATCAA 399920 0.0 83.7192 9 GCAGTGG 412985 0.0 83.52022 3 AAGCAGT 422100 0.0 82.19598 1 CAGTGGT 421930 0.0 80.981316 4 AGCAGTG 431710 0.0 80.544044 2 CAACGCA 384840 0.0 45.39153 12-13 ATCAACG 386815 0.0 45.23845 10-11 ACGCAGA 391790 0.0 44.910294 14-15 AGAGTAC 400470 0.0 43.729168 18-19 TCAACGC 385985 0.0 43.27068 12-13 AACGCAG 387530 0.0 42.85299 14-15 TATCAAC 392770 0.0 42.510662 10-11 CGCAGAG 393865 0.0 42.098618 16-17 AGTACTT 416730 0.0 41.830803 20-21 GAGTACT 393305 0.0 41.437523 20-21 GCAGAGT 430535 0.0 40.689537 16-17 >>END_MODULE