FastQCFastQC Report
Thu 26 May 2016
SRR1660695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55939463
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28834725.154629389273902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2637970.471575853347037No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTGCATCTCGTATG1699390.3037909033914037TruSeq Adapter, Index 14 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTTGCATCTCGTATG1228880.21968033550840488TruSeq Adapter, Index 4 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1096300.19597971471410086No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT643040.11495283749863669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3783150.084.8343058
AGTGGTA3832100.084.449425
GTGGTAT3819550.084.418876
TGGTATC3835500.083.622947
GTATCAA3834650.082.8585369
GCAGTGG3997200.081.988363
AAGCAGT4043650.080.993361
CAGTGGT4086750.079.7286454
AGCAGTG4177600.078.895462
CAACGCA3698400.044.84855712-13
ATCAACG3704150.044.68833510-11
ACGCAGA3780400.044.29997314-15
AGAGTAC3881850.043.087718-19
TCAACGC3706450.042.7033712-13
AACGCAG3727150.042.23602714-15
TATCAAC3774250.041.9788910-11
CGCAGAG3793950.041.4799616-17
AGTACTT4032800.041.4049420-21
GAGTACT3799800.040.99744420-21
GCAGAGT4185050.040.13121816-17
Produced by FastQC (version 0.11.5)