##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660695_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55939463 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30002626231861 34.0 31.0 34.0 31.0 34.0 2 32.660197399463776 34.0 31.0 34.0 31.0 34.0 3 32.813476829407534 34.0 31.0 34.0 31.0 34.0 4 36.25088694541097 37.0 37.0 37.0 35.0 37.0 5 36.17660961457567 37.0 37.0 37.0 35.0 37.0 6 36.182073610538595 37.0 37.0 37.0 35.0 37.0 7 36.1005117621526 37.0 36.0 37.0 35.0 37.0 8 36.13277072395207 37.0 36.0 37.0 35.0 37.0 9 37.896107779940614 39.0 38.0 39.0 35.0 39.0 10-11 37.7988486589512 39.0 37.5 39.0 35.0 39.0 12-13 37.85133374448017 39.0 38.0 39.0 35.0 39.0 14-15 39.33728712233079 41.0 39.0 41.0 36.0 41.0 16-17 39.27960043163088 41.0 39.0 41.0 36.0 41.0 18-19 39.22447311480269 41.0 39.0 41.0 36.0 41.0 20-21 39.19629074737453 40.5 39.0 41.0 36.0 41.0 22-23 39.05421634991383 40.0 38.5 41.0 35.5 41.0 24-25 39.03200522321782 40.0 39.0 41.0 35.0 41.0 26-27 38.990223198960635 40.0 39.0 41.0 35.0 41.0 28-29 38.888769516789964 40.0 38.5 41.0 35.0 41.0 30-31 38.77183152437484 40.0 38.0 41.0 35.0 41.0 32-33 38.632440143374275 40.0 38.0 41.0 35.0 41.0 34-35 38.53896855427447 40.0 38.0 41.0 34.0 41.0 36-37 38.375970546946434 40.0 38.0 41.0 34.0 41.0 38-39 37.964894881096725 40.0 37.0 41.0 33.5 41.0 40-41 37.844017889481705 40.0 37.0 41.0 33.0 41.0 42-43 37.82057922150594 40.0 36.5 41.0 33.0 41.0 44-45 37.89722151962739 40.0 37.0 41.0 33.0 41.0 46-47 37.787286874741724 40.0 36.5 41.0 33.0 41.0 48-49 37.67551837242342 40.0 36.0 41.0 33.0 41.0 50-51 37.50517361062261 40.0 36.0 41.0 33.0 41.0 52-53 37.09246332951033 40.0 35.5 41.0 31.5 41.0 54-55 36.64786616739599 40.0 35.0 41.0 30.5 41.0 56-57 36.28388747671747 39.0 35.0 41.0 28.5 41.0 58-59 36.060199916470424 39.0 35.0 41.0 28.0 41.0 60-61 35.901680518456175 39.0 35.0 41.0 28.0 41.0 62-63 35.71202695492447 39.0 35.0 41.0 28.0 41.0 64-65 35.434601383284644 38.0 35.0 40.5 27.0 41.0 66-67 35.17461052495266 37.5 35.0 40.0 27.0 41.0 68-69 34.89537202922381 37.0 34.5 40.0 26.0 41.0 70-71 34.51319808879825 36.5 34.0 39.5 26.0 41.0 72-73 32.808520373175554 35.5 33.0 39.0 12.5 41.0 74-75 31.932607942625406 35.0 31.5 38.0 11.0 40.0 76-77 31.56404358940664 34.5 30.0 36.0 21.5 39.0 78-79 32.892370257469224 35.0 33.0 37.0 24.0 39.0 80-81 32.90920374047924 35.0 34.0 37.0 24.5 39.0 82-83 32.65985738904931 35.0 34.0 36.0 24.0 38.5 84-85 32.416434449862344 35.0 33.5 36.0 24.0 37.0 86-87 32.128641420815924 35.0 33.0 35.5 22.0 37.0 88-89 31.947259727895492 35.0 33.0 35.0 22.5 36.5 90-91 31.836098605379892 35.0 33.0 35.0 22.5 36.0 92-93 31.714223606329575 35.0 33.0 35.0 20.5 36.0 94-95 31.594773478608474 35.0 33.0 35.0 20.0 36.0 96-97 31.47069700150679 35.0 33.0 35.0 20.0 35.0 98-99 31.38162742999517 35.0 33.0 35.0 19.5 35.0 100 31.327534838866793 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5532199149991399 1101 2 0.4018802993357937 1101 3 0.32020757367600794 1101 4 0.22750198214926343 1101 5 0.2176336705695121 1101 6 0.21258902074738728 1101 7 0.2525127172159145 1101 8 0.26158900921959827 1101 9 0.293852197922277 1101 10-11 0.307902106671591 1101 12-13 0.3057725684914345 1101 14-15 0.3439116682008674 1101 16-17 0.4069096653197093 1101 18-19 0.4094155807915101 1101 20-21 0.3662078660671071 1101 22-23 0.36008036527091036 1101 24-25 0.38444686498129244 1101 26-27 0.36909432351904314 1101 28-29 0.37082305975405916 1101 30-31 0.3413117224945452 1101 32-33 0.42737375314471393 1101 34-35 0.3649781234300349 1101 36-37 0.355989866120062 1101 38-39 0.3776709954868309 1101 40-41 0.38136526366869106 1101 42-43 0.3591598650601924 1101 44-45 0.3597265779034373 1101 46-47 0.3141725393474033 1101 48-49 0.2851191492675582 1101 50-51 0.27403266588625996 1101 52-53 0.19389289501381768 1101 54-55 0.15822433735231556 1101 56-57 0.21210126913730676 1101 58-59 0.2665485183357603 1101 60-61 0.20594715515765927 1101 62-63 0.23190088690501653 1101 64-65 0.2380006031703985 1101 66-67 0.21511197988803588 1101 68-69 0.24389578329194705 1101 70-71 0.2277437957557069 1101 72-73 1.5420686843359945 1101 74-75 2.087221501131456 1101 76-77 0.6835103430926601 1101 78-79 0.41649963224140407 1101 80-81 0.31871829319563005 1101 82-83 0.28694431349461524 1101 84-85 0.27507239688432605 1101 86-87 0.2883710628983138 1101 88-89 0.2736981320953866 1101 90-91 0.21615213045549808 1101 92-93 0.16145094336917865 1101 94-95 0.13483940638564818 1101 96-97 0.17291708181684484 1101 98-99 0.16009803257387745 1101 100 0.14544495179055872 1102 1 0.5292617723874429 1102 2 0.3952425521755458 1102 3 0.30090240979168925 1102 4 0.22118935095530645 1102 5 0.1792483601918562 1102 6 0.19732095109980463 1102 7 0.2335723656875004 1102 8 0.2353979170414462 1102 9 0.2788339242170679 1102 10-11 0.2869385265257307 1102 12-13 0.28288557498872535 1102 14-15 0.3312802332957645 1102 16-17 0.337796440940636 1102 18-19 0.3657511963805291 1102 20-21 0.32513781912890494 1102 22-23 0.31110766330343864 1102 24-25 0.34453034707228625 1102 26-27 0.31066519633493783 1102 28-29 0.3300843255469772 1102 30-31 0.30528668527086467 1102 32-33 0.33867578473562077 1102 34-35 0.319223648980838 1102 36-37 0.3018363701204194 1102 38-39 0.35593642497992306 1102 40-41 0.383625923625857 1102 42-43 0.340214630394307 1102 44-45 0.3760356032243095 1102 46-47 0.3219788754578161 1102 48-49 0.303414511036344 1102 50-51 0.2933812688070603 1102 52-53 0.18808286283925923 1102 54-55 0.221717923848324 1102 56-57 0.27955172416259444 1102 58-59 0.3470263797099875 1102 60-61 0.30714244001066504 1102 62-63 0.3002357058198797 1102 64-65 0.24165514342467276 1102 66-67 0.2602236621808913 1102 68-69 0.24893883149876928 1102 70-71 0.292937613382243 1102 72-73 1.589938654836061 1102 74-75 2.063840774743273 1102 76-77 0.6715397699519166 1102 78-79 0.443377325708191 1102 80-81 0.34015494382142464 1102 82-83 0.31825970394413616 1102 84-85 0.2958336856478567 1102 86-87 0.2917543650511121 1102 88-89 0.26337924585373784 1102 90-91 0.1992931878792774 1102 92-93 0.19678356667331087 1102 94-95 0.2239032872284703 1102 96-97 0.23580399580425038 1102 98-99 0.21141397690791308 1102 100 0.21913239128064177 1103 1 0.49122649504924 1103 2 0.3553470354609587 1103 3 0.26546374365950953 1103 4 0.17964836187517363 1103 5 0.2055011829179776 1103 6 0.1769725945651075 1103 7 0.21688764061610044 1103 8 0.20516956520668117 1103 9 0.22923513114101723 1103 10-11 0.23068404446677704 1103 12-13 0.22899549812245112 1103 14-15 0.2595347623333879 1103 16-17 0.2922426996882237 1103 18-19 0.3143826282994553 1103 20-21 0.2824445994009608 1103 22-23 0.28191099076359905 1103 24-25 0.2782658977632835 1103 26-27 0.2608895390154444 1103 28-29 0.31414250214324824 1103 30-31 0.2503054049821358 1103 32-33 0.3045465678767698 1103 34-35 0.28112708851930535 1103 36-37 0.2633792889779798 1103 38-39 0.30265779652922475 1103 40-41 0.30586945824706646 1103 42-43 0.25975474385455044 1103 44-45 0.28123352519848765 1103 46-47 0.2453882852548901 1103 48-49 0.2051044331210008 1103 50-51 0.22317107586339802 1103 52-53 0.1286190630609667 1103 54-55 0.12421245735514219 1103 56-57 0.1841608773849046 1103 58-59 0.2568874334747946 1103 60-61 0.1990319873893398 1103 62-63 0.18513727465055752 1103 64-65 0.17802121503137158 1103 66-67 0.16786329919125365 1103 68-69 0.19562735688909072 1103 70-71 0.1833835188356332 1103 72-73 1.4883439888727423 1103 74-75 1.9607036975044885 1103 76-77 0.5272430993722388 1103 78-79 0.3359966178049305 1103 80-81 0.2456821293873972 1103 82-83 0.19946761568092342 1103 84-85 0.16843588718311509 1103 86-87 0.2362650271981508 1103 88-89 0.18058721058481453 1103 90-91 0.14757279036481918 1103 92-93 0.12263086151317282 1103 94-95 0.12246235257452653 1103 96-97 0.12635168527962648 1103 98-99 0.11292528947653935 1103 100 0.15480771798820214 1104 1 0.42095105683056744 1104 2 0.30326484290230127 1104 3 0.20341663099960527 1104 4 0.149296921927629 1104 5 0.13996670692865365 1104 6 0.1375651048499691 1104 7 0.18204076932880042 1104 8 0.17769106187493122 1104 9 0.21832664249001965 1104 10-11 0.19847149811002396 1104 12-13 0.19432731615168564 1104 14-15 0.22342503082931842 1104 16-17 0.25647730962752746 1104 18-19 0.26508432687328565 1104 20-21 0.22426336508156197 1104 22-23 0.21715163775790103 1104 24-25 0.2539112223922828 1104 26-27 0.2452417152669355 1104 28-29 0.24388903913219195 1104 30-31 0.214082891169042 1104 32-33 0.2632253868631338 1104 34-35 0.27720443038177933 1104 36-37 0.2423294494426571 1104 38-39 0.2729481033821628 1104 40-41 0.28033985844097487 1104 42-43 0.22484237091806847 1104 44-45 0.2347515481290685 1104 46-47 0.2086124877307114 1104 48-49 0.19373956761023692 1104 50-51 0.1860174999633557 1104 52-53 0.0980438121354652 1104 54-55 0.09741410083139357 1104 56-57 0.09827859193893573 1104 58-59 0.15309268385595232 1104 60-61 0.11846720375017128 1104 62-63 0.1221131717046049 1104 64-65 0.09738556434890455 1104 66-67 0.11284256465361864 1104 68-69 0.13369617898742803 1104 70-71 0.13181217121364597 1104 72-73 1.4293572699657915 1104 74-75 1.931069055671113 1104 76-77 0.5281324521018043 1104 78-79 0.3169213333406873 1104 80-81 0.19895232832360676 1104 82-83 0.1694319403651292 1104 84-85 0.11984068602716746 1104 86-87 0.20169776387235316 1104 88-89 0.20352423052878166 1104 90-91 0.13524410852337354 1104 92-93 0.09271314318835877 1104 94-95 0.09683897519215279 1104 96-97 0.07924185300507602 1104 98-99 0.11232049448684478 1104 100 0.1108761847905484 1105 1 0.38010919765167017 1105 2 0.24505599174712245 1105 3 0.17188853893943445 1105 4 0.13267845874596418 1105 5 0.10851214242453722 1105 6 0.1134369801620494 1105 7 0.11416095807344817 1105 8 0.11806959403050854 1105 9 0.1179417779864167 1105 10-11 0.15608226307161033 1105 12-13 0.13119449478186596 1105 14-15 0.15702675953078682 1105 16-17 0.18581270518496495 1105 18-19 0.21412022600767955 1105 20-21 0.17639934076220243 1105 22-23 0.14344754581731678 1105 24-25 0.20001491845153652 1105 26-27 0.16573900381416706 1105 28-29 0.19706745145704474 1105 30-31 0.15601238805529505 1105 32-33 0.21603154400562374 1105 34-35 0.18936069325710747 1105 36-37 0.17092118560118053 1105 38-39 0.19406482768875577 1105 40-41 0.19623178035231348 1105 42-43 0.18938735666453255 1105 44-45 0.17477730764698407 1105 46-47 0.1578899048513165 1105 48-49 0.12224704362368044 1105 50-51 0.10789949734947868 1105 52-53 0.06015452250340303 1105 54-55 0.025203468748351554 1105 56-57 0.05557149937882855 1105 58-59 0.14500090043036806 1105 60-61 0.0780338194947916 1105 62-63 0.052683743228762125 1105 64-65 0.08140208078621214 1105 66-67 0.08839486314576561 1105 68-69 0.1211114691170323 1105 70-71 0.1359636622527347 1105 72-73 1.4484818352137125 1105 74-75 1.9348500097628225 1105 76-77 0.4843021254495419 1105 78-79 0.2604863599050944 1105 80-81 0.17695482103173532 1105 82-83 0.1493252152759439 1105 84-85 0.13890168898570465 1105 86-87 0.2064580169157395 1105 88-89 0.1870086682860439 1105 90-91 0.09612985026107168 1105 92-93 0.10277075187860518 1105 94-95 0.12053120939752304 1105 96-97 0.08744586361445883 1105 98-99 0.0679093204146497 1105 100 0.05049747576450869 1106 1 0.34165331102607865 1106 2 0.21515426581481734 1106 3 0.12964265243466144 1106 4 0.08570936292579034 1106 5 0.07989183511791964 1106 6 0.07800263021442788 1106 7 0.0851799785564964 1106 8 0.10716440171079711 1106 9 0.10275293131260099 1106 10-11 0.11439912685300158 1106 12-13 0.11194084317280328 1106 14-15 0.07867395019904677 1106 16-17 0.1022723501742604 1106 18-19 0.14752596541174512 1106 20-21 0.08711092611001447 1106 22-23 0.0708491762808734 1106 24-25 0.12564187353142842 1106 26-27 0.11085849708162954 1106 28-29 0.10640751543957805 1106 30-31 0.0878133933214329 1106 32-33 0.15851025834509613 1106 34-35 0.15483503226179351 1106 36-37 0.1185900084248459 1106 38-39 0.15379711568076715 1106 40-41 0.1543730160695631 1106 42-43 0.1647238499537167 1106 44-45 0.1441971360041805 1106 46-47 0.11142113694274514 1106 48-49 0.04455140581632122 1106 50-51 0.02388455508797449 1106 52-53 0.024150445874063564 1106 54-55 -0.03729736330418376 1106 56-57 -0.036454228644466014 1106 58-59 0.02872593960672276 1106 60-61 0.018462849864882003 1106 62-63 0.01919484621719647 1106 64-65 0.05435119439039937 1106 66-67 0.04608908420664193 1106 68-69 0.06890375969498308 1106 70-71 0.10910167722348518 1106 72-73 1.4801443913423356 1106 74-75 1.9394919607025791 1106 76-77 0.4578278668917797 1106 78-79 0.30901766084293314 1106 80-81 0.16167487565770244 1106 82-83 0.15069297668130588 1106 84-85 0.12277850545489599 1106 86-87 0.2124737258780769 1106 88-89 0.18689452168890952 1106 90-91 0.10135644009907452 1106 92-93 0.0746861274176176 1106 94-95 0.10066383333649398 1106 96-97 0.12244439286922315 1106 98-99 0.11102184147591032 1106 100 0.10447981369168247 1107 1 0.33183710971157865 1107 2 0.20640381168795585 1107 3 0.12250985286770799 1107 4 0.08558578320833732 1107 5 0.09363902150118975 1107 6 0.08754045209307293 1107 7 0.08837013338251865 1107 8 0.0890108684822124 1107 9 0.10879942277338017 1107 10-11 0.07944100473887517 1107 12-13 0.10034707864879522 1107 14-15 0.10912996550726461 1107 16-17 0.13028526472749746 1107 18-19 0.12266049218935393 1107 20-21 0.06766153886962911 1107 22-23 0.10143259676124927 1107 24-25 0.10459437508993119 1107 26-27 0.07874992296106598 1107 28-29 0.09797126986614302 1107 30-31 0.10526324780423835 1107 32-33 0.11779838034750867 1107 34-35 0.07023247501235375 1107 36-37 0.08775829728282503 1107 38-39 0.1140320453282726 1107 40-41 0.13225187538710514 1107 42-43 0.07303371643177314 1107 44-45 0.12391726824150595 1107 46-47 0.09033642248626705 1107 48-49 0.09751575753878683 1107 50-51 0.05614282671972859 1107 52-53 0.018642915595101783 1107 54-55 0.02276403010967698 1107 56-57 0.004026711959426166 1107 58-59 0.06809204585118067 1107 60-61 -0.018885745986040092 1107 62-63 -0.02030663021396606 1107 64-65 0.02703900083965749 1107 66-67 0.022187506300753057 1107 68-69 0.036797262158351884 1107 70-71 0.06351048673629833 1107 72-73 1.4040041635200708 1107 74-75 1.8503171463408883 1107 76-77 0.4039063393332434 1107 78-79 0.20137254463035248 1107 80-81 0.10512552534967767 1107 82-83 0.09080435421803656 1107 84-85 0.05485868543205896 1107 86-87 0.14440300619551039 1107 88-89 0.12139341236564505 1107 90-91 0.05773007103071137 1107 92-93 0.03842763999275789 1107 94-95 0.029288863900749362 1107 96-97 0.05418348364960579 1107 98-99 0.07965648780686507 1107 100 0.10166681459991977 1108 1 0.2793050310168823 1108 2 0.14479117901049676 1108 3 0.0765137483768683 1108 4 0.042498517861666585 1108 5 0.05055461514467652 1108 6 0.029260183911041793 1108 7 0.04453561277458107 1108 8 0.03563218584039163 1108 9 0.016222892245387754 1108 10-11 0.04788224396172325 1108 12-13 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54-55 -1.2959877840508014 2316 56-57 -1.0972440227003304 2316 58-59 -0.925380499421486 2316 60-61 -0.928028369059966 2316 62-63 -0.9760553537735603 2316 64-65 -1.1076425414133908 2316 66-67 -1.0010131704041498 2316 68-69 -0.9873473848391114 2316 70-71 -2.2239541065885717 2316 72-73 -0.44263999225471196 2316 74-75 0.8251774070828937 2316 76-77 -0.3267996360095218 2316 78-79 -1.7882274880589222 2316 80-81 -1.6085901520992358 2316 82-83 -0.8722408344901922 2316 84-85 -0.7374426733193076 2316 86-87 -0.6905867701285757 2316 88-89 -0.8389070321798577 2316 90-91 -0.962678357837305 2316 92-93 -0.890713673354238 2316 94-95 -0.9061891362966605 2316 96-97 -0.9221797363052495 2316 98-99 -0.9095962215058861 2316 100 -0.9327090118687771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 52.0 7 1100.0 8 2179.0 9 2773.0 10 10353.0 11 14313.0 12 10422.0 13 10039.0 14 14524.0 15 26028.0 16 43489.0 17 66447.0 18 92646.0 19 129406.0 20 199977.0 21 214330.0 22 253856.0 23 393735.0 24 650370.0 25 918698.0 26 964478.0 27 818159.0 28 741782.0 29 807350.0 30 921144.0 31 1036514.0 32 1253786.0 33 1608099.0 34 2254192.0 35 3642273.0 36 6246499.0 37 1.221103E7 38 1.5569424E7 39 4801236.0 40 8757.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.04646098189589 35.86535176190042 27.134884441457164 19.953302814746525 2 20.455851006529958 33.24291647081491 28.295966540088063 18.00526598256707 3 24.707755953967595 27.742093269647583 27.1277470075106 20.42240376887422 4 18.970790929331187 27.588048577211104 27.623221188746317 25.81793930471139 5 20.001071525622415 33.161168805275565 26.7648526653163 20.072907003785716 6 26.513699282383175 26.904110609713932 27.12287209478575 19.459318013117144 7 20.3411623740471 27.212758549362547 32.983187557592395 19.462891518997957 8 25.463487198652583 27.472571554718 27.312972239293753 19.750969007335662 9 25.302382684653228 27.186172666691494 27.8006458517487 19.710798796906577 10-11 19.617537265239747 30.66297668964037 30.184930448831803 19.53455559628808 12-13 19.668577876155585 27.425145576164628 29.852691068838972 23.053585478840816 14-15 19.361291223555018 33.86770349748598 26.755913243175595 20.015092035783415 16-17 22.662510914801626 27.542603647565834 26.732881319833158 23.062004117799383 18-19 19.96565823307957 30.455598939160357 26.99862617558556 22.58011665217451 20-21 22.671057818341946 30.4480684771679 27.02359513175877 19.85727857273138 22-23 22.751563596525763 27.777048020643313 29.89316683286717 19.578221549963754 24-25 19.639655975774538 30.538792827773186 26.818806728930113 23.002744467522163 26-27 19.51934722511789 27.267065697665466 33.32062596808459 19.892961109132052 28-29 19.53631229003266 27.62913518661116 33.001602643230456 19.832949880125728 30-31 19.856363989540995 27.33724830156853 33.23517300006145 19.571214708829025 32-33 19.621882240947194 27.67745989945472 32.84317803187932 19.857479827718766 34-35 19.61752374746249 27.426446187985025 33.11748406207283 19.838546002479653 36-37 19.91248735196581 27.432532767045476 33.07877217435879 19.576207706629926 38-39 19.991509884982257 27.451094827763452 33.00526218567083 19.552133101583458 40-41 19.6790480072942 27.833251989665868 32.68138256801644 19.806317435023498 42-43 19.706319619497616 27.577320620113728 32.907155522926985 19.80920423746167 44-45 19.743458647735352 27.636854503596876 32.82837374287034 19.791313105797432 46-47 20.058585643426465 27.732088256082072 32.73774110373735 19.471584996754114 48-49 19.808774470383902 28.068817430001445 32.62496969066112 19.49743840895354 50-51 20.156763866248347 27.842571681359455 32.186299419485735 19.81436503290646 52-53 20.21856943279593 27.991544086703783 31.92884611877999 19.86104036172029 54-55 20.168337636693636 28.250835663140755 31.541199639038105 20.039627061127504 56-57 20.54238086687065 28.747554938674714 30.778713674524578 19.931350519930056 58-59 20.718290919843703 29.209818737256697 29.85268032253726 20.21921002036234 60-61 20.96754080899973 29.504060316020396 29.256892992173356 20.27150588280652 62-63 20.66450993490986 29.651993210775036 29.66990270438563 20.013594149929478 64-65 20.995413971175207 30.02709559173579 28.95555009918671 20.02194033790229 66-67 20.663675555130403 30.203276276710035 29.0558922471356 20.077155921023966 68-69 20.623090945433947 29.99872142920576 29.267126741392584 20.111060883967706 70-71 20.580041688497314 29.871410347293846 29.414173931004267 20.134374033204573 72-73 21.168195098918787 29.585375218360653 29.167514184414898 20.078915498305662 74-75 20.577718380846648 29.728015732094942 29.298249466427713 20.396016420630698 76-77 20.6416254204311 29.77234528207803 29.128109026423875 20.457920271066993 78-79 20.73658935196716 29.849455753502692 28.8226177719486 20.591337122581553 80-81 20.773427481901084 29.91756058262917 28.589588603653493 20.71942333181625 82-83 20.841292567711665 29.990454445134056 28.31523638936213 20.853016597792145 84-85 20.833373283115968 29.96230552674204 28.192041473131123 21.012279717010866 86-87 20.87028044158891 29.909192110242028 28.06252288424689 21.158004563922177 88-89 20.887264223960063 29.864183821952047 28.018212409825654 21.230339544262236 90-91 20.918303449072955 29.76510476775808 27.934698982490236 21.38189280067873 92-93 20.935524662553885 29.68210374069271 27.912766099756293 21.46960549699711 94-95 20.962747295637723 29.604075049419983 27.891976667131622 21.541200987810672 96-97 20.94218886380488 29.534037763551023 27.847237213313864 21.67653615933023 98-99 20.92759618966814 29.457226057452917 27.853047025513234 21.76213072736571 100 20.868415367161912 29.434935206298757 27.853035741641257 21.843613684898074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262866.0 1 161075.5 2 45075.0 3 28855.5 4 26553.5 5 26172.5 6 26709.5 7 27712.0 8 28389.5 9 29222.0 10 32119.0 11 46750.0 12 374346.0 13 440897.0 14 171705.0 15 158816.0 16 175959.5 17 195418.0 18 214806.0 19 232635.5 20 256373.0 21 293952.0 22 348653.0 23 412998.0 24 479083.0 25 556375.0 26 648456.0 27 754980.5 28 876533.5 29 995451.0 30 1091464.5 31 1177196.5 32 1273898.5 33 1359768.0 34 1423111.5 35 1493809.5 36 1587706.5 37 1681313.5 38 1751758.0 39 1785037.5 40 1791684.5 41 1807487.0 42 1842439.0 43 1850791.5 44 1852816.5 45 1867709.0 46 1854179.0 47 1829662.0 48 1788261.0 49 1732575.5 50 1690257.0 51 1640966.0 52 1563209.5 53 1448873.0 54 1315055.5 55 1194631.5 56 1085631.5 57 976365.0 58 856291.5 59 724410.5 60 591161.5 61 474138.0 62 369520.5 63 271396.5 64 193691.0 65 136454.5 66 96061.0 67 70794.5 68 51835.0 69 37240.5 70 27432.5 71 20955.0 72 16432.5 73 12517.0 74 9642.5 75 7902.0 76 5918.0 77 3720.0 78 2266.0 79 1495.0 80 1014.0 81 687.5 82 449.0 83 272.5 84 179.0 85 140.5 86 98.5 87 62.5 88 34.5 89 20.5 90 16.5 91 16.0 92 11.5 93 3.5 94 1.5 95 1.5 96 2.0 97 2.5 98 3.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20501626910505022 2 8.044410437046921E-5 3 0.0 4 0.0018806043955051912 5 9.474527848077484E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.0389994984399474E-5 14-15 4.4691169094705106E-5 16-17 0.0019476411491472485 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005562262905526998 26-27 0.03186659121128853 28-29 0.056331430997112006 30-31 0.08838036217830693 32-33 0.09296835759756936 34-35 0.10042373842594807 36-37 0.10766281399590841 38-39 0.09948254240481358 40-41 0.0601659333054377 42-43 0.04274799706246733 44-45 0.04679522933568383 46-47 0.08847153216326013 48-49 0.11726068947068727 50-51 0.13212586613496807 52-53 0.4924216022595712 54-55 0.47847885132540513 56-57 0.4842189850839291 58-59 0.4769897415711695 60-61 0.4954820535191766 62-63 0.4838516236739705 64-65 0.463666410240656 66-67 0.469659496016256 68-69 0.47895883448148224 70-71 0.4867923026004022 72-73 1.1761401785355001 74-75 3.1631274329537273 76-77 0.48526297079398134 78-79 0.4957546696506543 80-81 0.5201372776853435 82-83 0.53152726904082 84-85 0.5303778121717043 86-87 0.5034621086727271 88-89 0.4811996496998908 90-91 0.454593209091049 92-93 0.4419742105854681 94-95 0.44590882111256597 96-97 0.465793709889564 98-99 0.45907930864477553 100 0.4600562576011858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.5939463E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.072156542773048 #Duplication Level Percentage of deduplicated Percentage of total 1 63.98428305112846 17.96176810088378 2 15.843636509193114 8.895300885857269 3 6.75966728105934 5.692753142728764 4 3.3934018496787495 3.810404317468699 5 2.039843914316803 2.8631408842762114 6 1.3458383629479083 2.2668351123565857 7 0.9211386913853873 1.8100844679522992 8 0.707432708580127 1.5887329390991418 9 0.5472787729735484 1.3826965848705184 >10 3.8059186794614437 20.80042559908972 >50 0.3652099702436651 7.325532632558921 >100 0.26333707942720913 14.262882792063847 >500 0.021881398754865923 3.915440985243108 >1k 9.153140986873169E-4 0.5104232657695986 >5k 1.3366934286284434E-4 0.2546118218987571 >10k+ 8.274740790846005E-5 6.658966467882779 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2883472 5.154629389273902 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 263797 0.471575853347037 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTGCATCTCGTATG 169939 0.3037909033914037 TruSeq Adapter, Index 14 (97% over 37bp) AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTTGCATCTCGTATG 122888 0.21968033550840488 TruSeq Adapter, Index 4 (97% over 35bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109630 0.19597971471410086 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 64304 0.11495283749863669 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.58612110738353 0.0 0.0 0.0 5.1841756149857925E-5 2 0.5861336209108765 0.0 0.0 0.0 5.1841756149857925E-5 3 0.5861407714979316 3.5752935275764087E-6 0.0 0.0 5.1841756149857925E-5 4 0.5861443467914592 3.5752935275764087E-6 0.0 0.0 5.1841756149857925E-5 5 0.5861532850252781 3.5752935275764087E-6 0.0 0.0 5.1841756149857925E-5 6 0.5863195361743104 7.150587055152817E-6 0.0 0.0 5.5417049677434334E-5 7 0.5863284744081294 1.0725880582729227E-5 0.0 0.0 5.5417049677434334E-5 8 0.5863284744081294 1.0725880582729227E-5 0.0 1.7876467637882043E-6 5.5417049677434334E-5 9 0.5863302620548931 1.251352734651743E-5 0.0 1.7876467637882043E-6 5.5417049677434334E-5 10-11 0.586340987935476 1.6982644255987942E-5 0.0 2.6814701456823063E-6 5.5417049677434334E-5 12-13 0.5863651211667871 2.2345584547352556E-5 0.0 3.5752935275764087E-6 5.5417049677434334E-5 14-15 0.5864464590945394 2.323940792924666E-5 0.0 3.5752935275764087E-6 5.5417049677434334E-5 16-17 0.586483105853197 3.0389994984399474E-5 0.0 3.5752935275764087E-6 5.5417049677434334E-5 18-19 0.5865063452611263 3.3965288511975884E-5 0.0 5.3629402913646135E-6 5.5417049677434334E-5 20-21 0.5865617623108037 4.20096989490228E-5 0.0 5.3629402913646135E-6 5.5417049677434334E-5 22-23 0.5867154999324895 4.3797345712811013E-5 0.0 5.3629402913646135E-6 5.5417049677434334E-5 24-25 0.5873715662947998 4.647881585849332E-5 0.0 5.3629402913646135E-6 5.720469644122254E-5 26-27 0.5895212115282551 4.916028600417562E-5 0.0 5.3629402913646135E-6 6.435528349637536E-5 28-29 0.5959710410520029 5.631087305932844E-5 0.0 6.256763673258715E-6 6.793057702395177E-5 30-31 0.6106458333359404 6.256763673258716E-5 0.0 1.0725880582729227E-5 6.971822378773997E-5 32-33 0.6399596649685393 7.329351731531638E-5 0.0 1.0725880582729227E-5 7.329351731531638E-5 34-35 0.6894667544448898 7.508116407910459E-5 0.0 1.0725880582729227E-5 7.597498746099869E-5 36-37 0.7646149552776365 7.686881084289278E-5 0.0 1.1619703964623328E-5 8.133792775236331E-5 38-39 0.8686819178081848 8.044410437046921E-5 0.0 1.251352734651743E-5 8.22317511342574E-5 40-41 1.0052259529198555 8.848851480751613E-5 0.0 1.251352734651743E-5 8.22317511342574E-5 42-43 1.1773200253996001 9.027616157130432E-5 0.0 1.251352734651743E-5 8.40193978980456E-5 44-45 1.386544414986608 9.206380833509252E-5 0.0 1.4301174110305635E-5 8.40193978980456E-5 46-47 1.635283842463772 9.563910186266894E-5 0.0 1.608882087409384E-5 8.40193978980456E-5 48-49 1.9245831873645267 1.0010821877213944E-4 0.0 1.6982644255987942E-5 8.40193978980456E-5 50-51 2.2539061556597355 1.0189586553592765E-4 0.0 2.1451761165458454E-5 8.759469142562203E-5 52-53 2.6211290229940176 1.0725880582729226E-4 0.0 2.6814701456823065E-5 9.295763171698663E-5 54-55 3.0261543626187475 1.1083409935486868E-4 0.0 2.860234822061127E-5 9.295763171698663E-5 56-57 3.468732797810376 1.1440939288244508E-4 0.0 3.0389994984399474E-5 9.295763171698663E-5 58-59 3.9481769783882266 1.1887850979191559E-4 0.0 3.0389994984399474E-5 9.295763171698663E-5 60-61 4.458525996218448 1.251352734651743E-4 0.0 3.0389994984399474E-5 9.295763171698663E-5 62-63 4.97414231523817 1.2871056699275072E-4 0.0 3.0389994984399474E-5 9.653292524456304E-5 64-65 5.490011586274971 1.3586115404790354E-4 0.0 3.0389994984399474E-5 9.653292524456304E-5 66-67 6.034935480163619 1.3675497742979763E-4 0.0 3.3965288511975884E-5 9.832057200835124E-5 68-69 6.603220341961452 1.3764880081169173E-4 0.0 3.3965288511975884E-5 1.0100204215403354E-4 70-71 7.19835833962153 1.3943644757547993E-4 0.0 3.575293527576409E-5 1.0547115906350406E-4 72-73 7.821904725828348 1.4301174110305633E-4 0.0 4.11158755671287E-5 1.0547115906350406E-4 74-75 8.498066919233743 1.4748085801252688E-4 0.0 4.3797345712811013E-5 1.0636498244539816E-4 76-77 9.22293801783546 1.5641909183146787E-4 0.0 4.469116909470511E-5 1.0725880582729226E-4 78-79 9.966179868405245 1.591005619771502E-4 0.0 4.469116909470511E-5 1.0725880582729226E-4 80-81 10.72958726829394 1.653573256504089E-4 0.0 4.916028600417562E-5 1.0725880582729226E-4 82-83 11.51089437522845 1.7340173608745583E-4 0.0 5.720469644122254E-5 1.0904645259108047E-4 84-85 12.307976571030007 1.7876467637882045E-4 0.0 6.077998996879895E-5 1.1083409935486867E-4 86-87 13.124084691338563 1.8233996990639685E-4 0.0 6.614293026016357E-5 1.1440939288244508E-4 88 13.746388663044549 1.8233996990639685E-4 0.0 6.971822378773997E-5 1.1440939288244508E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 378315 0.0 84.834305 8 AGTGGTA 383210 0.0 84.44942 5 GTGGTAT 381955 0.0 84.41887 6 TGGTATC 383550 0.0 83.62294 7 GTATCAA 383465 0.0 82.858536 9 GCAGTGG 399720 0.0 81.98836 3 AAGCAGT 404365 0.0 80.99336 1 CAGTGGT 408675 0.0 79.728645 4 AGCAGTG 417760 0.0 78.89546 2 CAACGCA 369840 0.0 44.848557 12-13 ATCAACG 370415 0.0 44.688335 10-11 ACGCAGA 378040 0.0 44.299973 14-15 AGAGTAC 388185 0.0 43.0877 18-19 TCAACGC 370645 0.0 42.70337 12-13 AACGCAG 372715 0.0 42.236027 14-15 TATCAAC 377425 0.0 41.97889 10-11 CGCAGAG 379395 0.0 41.47996 16-17 AGTACTT 403280 0.0 41.40494 20-21 GAGTACT 379980 0.0 40.997444 20-21 GCAGAGT 418505 0.0 40.131218 16-17 >>END_MODULE