FastQCFastQC Report
Thu 26 May 2016
SRR1660694_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660694_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28975340
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21460117.406335870433272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2471720.8530426217604349No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT736980.2543473174085274No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT430790.14867470062473814No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC380440.13129785534872065Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2734250.087.3339848
AGTGGTA2755250.087.182955
AAGCAGT2810250.087.078281
TGGTATC2749050.086.641997
GTGGTAT2763450.086.618186
GCAGTGG2834800.086.007913
GTATCAA2775200.085.268529
AGCAGTG2927800.083.873872
CAGTGGT2897950.083.3601464
CAACGCA2709500.045.65993512-13
ATCAACG2713650.045.57822410-11
ACGCAGA2759950.045.13771414-15
AGAGTAC2776100.044.64389818-19
TCAACGC2711150.043.6057312-13
AACGCAG2730800.043.11955614-15
AGTACTT2857400.043.1193420-21
TATCAAC2740550.043.08104710-11
CGCAGAG2766500.042.43728616-17
GAGTACT2749950.041.94006720-21
GCAGAGT2970500.041.75624516-17
Produced by FastQC (version 0.11.5)