##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660694_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28975340 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20840248984136 34.0 31.0 34.0 30.0 34.0 2 32.356437819193836 34.0 31.0 34.0 30.0 34.0 3 32.33487144585706 34.0 31.0 34.0 30.0 34.0 4 35.738072926840545 37.0 35.0 37.0 35.0 37.0 5 35.762549119354595 37.0 35.0 37.0 35.0 37.0 6 35.77496191589124 37.0 35.0 37.0 35.0 37.0 7 35.68936826970796 37.0 35.0 37.0 35.0 37.0 8 35.76719213648572 37.0 35.0 37.0 35.0 37.0 9 37.45531852257817 39.0 38.0 39.0 35.0 39.0 10-11 37.339454998629876 39.0 37.0 39.0 34.5 39.0 12-13 37.42797304535512 39.0 38.0 39.0 35.0 39.0 14-15 38.85552965038546 41.0 39.0 41.0 35.0 41.0 16-17 38.78236811026204 40.5 38.5 41.0 35.0 41.0 18-19 38.63953969133753 40.0 38.0 41.0 34.0 41.0 20-21 38.55117896804662 40.0 38.0 41.0 34.0 41.0 22-23 38.33383597914641 40.0 38.0 41.0 33.5 41.0 24-25 38.36729672542238 40.0 38.0 41.0 34.0 41.0 26-27 38.38587134784268 40.0 38.0 41.0 34.0 41.0 28-29 38.318954238328175 40.0 38.0 41.0 34.0 41.0 30-31 38.21775583996599 40.0 38.0 41.0 33.5 41.0 32-33 38.096549427892825 40.0 38.0 41.0 33.0 41.0 34-35 37.982660565846686 40.0 38.0 41.0 33.0 41.0 36-37 37.77401429974592 40.0 37.5 41.0 33.0 41.0 38-39 37.347984303204036 40.0 35.0 41.0 32.5 41.0 40-41 37.38400199963141 40.0 35.0 41.0 33.0 41.0 42-43 37.25517131119083 40.0 35.0 41.0 32.0 41.0 44-45 37.12412187397973 40.0 35.0 41.0 32.0 41.0 46-47 37.20484658678725 40.0 35.0 41.0 32.0 41.0 48-49 37.151425022105 40.0 35.0 41.0 32.0 41.0 50-51 35.757607900373216 38.5 34.0 40.0 29.0 40.5 52-53 35.85286093277939 38.5 35.0 39.5 29.0 40.5 54-55 36.13249368255903 39.0 35.0 41.0 27.5 41.0 56-57 35.66808891629917 39.0 35.0 41.0 23.5 41.0 58-59 35.45809429673646 39.0 35.0 41.0 23.0 41.0 60-61 35.3206483858343 39.0 35.0 41.0 23.5 41.0 62-63 35.132210424450584 39.0 35.0 41.0 23.0 41.0 64-65 34.89103106296595 38.0 35.0 41.0 23.0 41.0 66-67 34.62300250833985 37.5 34.0 40.0 23.0 41.0 68-69 34.385228439079576 37.0 34.0 40.0 23.0 41.0 70-71 34.07990672413162 36.0 34.0 39.5 23.0 41.0 72-73 33.757483121854655 36.0 34.0 39.0 22.5 41.0 74-75 33.36059695934543 35.0 33.0 39.0 21.0 40.5 76-77 32.95131365153955 35.0 33.0 37.5 20.0 40.0 78-79 32.54489222559597 35.0 33.0 37.0 19.5 39.0 80-81 32.134690619678665 35.0 33.0 36.5 18.5 39.0 82-83 31.826143489601847 35.0 32.0 36.0 18.0 38.5 84-85 31.51805010743619 35.0 32.0 36.0 17.0 37.0 86-87 31.24623711059128 35.0 32.0 35.0 15.5 37.0 88-89 31.063400740077597 35.0 32.0 35.0 15.0 36.5 90-91 30.858467303576077 35.0 31.0 35.0 15.0 36.0 92-93 30.690217785192512 35.0 31.0 35.0 14.5 36.0 94-95 30.529163195324024 35.0 31.0 35.0 12.0 36.0 96-97 30.385729434063585 35.0 31.0 35.0 10.5 35.0 98-99 30.25856217735495 35.0 31.0 35.0 10.0 35.0 100 30.15035923650939 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33695991065222586 1101 2 0.3247862140752531 1101 3 0.3052021529386053 1101 4 0.2660063255502294 1101 5 0.28318864481959594 1101 6 0.1968454968758806 1101 7 0.22569708339369043 1101 8 0.2317806842709942 1101 9 0.32024093481353333 1101 10-11 0.3151123227007204 1101 12-13 0.3251466289156042 1101 14-15 0.39653691007848835 1101 16-17 0.3486339875225042 1101 18-19 0.42373704169169457 1101 20-21 0.38102562361011394 1101 22-23 0.3829357967418403 1101 24-25 0.36298436240376475 1101 26-27 0.3583917245437718 1101 28-29 0.35901651468100226 1101 30-31 0.3257773535818913 1101 32-33 0.3563282465078146 1101 34-35 0.3633280641842376 1101 36-37 0.33123765494040214 1101 38-39 0.3178552131038188 1101 40-41 0.30939423410212896 1101 42-43 0.3237150457105429 1101 44-45 0.3061923068509884 1101 46-47 0.36273144148301384 1101 48-49 0.3619714233554703 1101 50-51 0.2688172177739787 1101 52-53 0.35103684400814217 1101 54-55 0.3908605435221162 1101 56-57 0.4056012370759632 1101 58-59 0.41481496797172923 1101 60-61 0.4081952312017023 1101 62-63 0.33842632118211924 1101 64-65 0.3686271819093605 1101 66-67 0.3570773016971671 1101 68-69 0.30391677843331877 1101 70-71 0.33597130812754017 1101 72-73 0.26907299862318723 1101 74-75 0.31545104348623454 1101 76-77 0.29688884355442724 1101 78-79 0.292033325569264 1101 80-81 0.27358147111296915 1101 82-83 0.18044845821031075 1101 84-85 0.21171175867989334 1101 86-87 0.28899666746822916 1101 88-89 0.23253296735340712 1101 90-91 0.16928514099769032 1101 92-93 0.16473156902879182 1101 94-95 0.16069274259625033 1101 96-97 0.17440168446384519 1101 98-99 0.1019079996608987 1101 100 0.0841520669876239 1102 1 0.35651959173462444 1102 2 0.3207894525072277 1102 3 0.3435395990143135 1102 4 0.27412979193205445 1102 5 0.28853541195702803 1102 6 0.21451341380821276 1102 7 0.23014250340493447 1102 8 0.2284107209415751 1102 9 0.33411938364002225 1102 10-11 0.32715212337811295 1102 12-13 0.3117159461221064 1102 14-15 0.3676166813238737 1102 16-17 0.3779003339062328 1102 18-19 0.3990807450152829 1102 20-21 0.3793748038248026 1102 22-23 0.3587936115212855 1102 24-25 0.3789516565028421 1102 26-27 0.37346821651102147 1102 28-29 0.3576212976582127 1102 30-31 0.36432415861765577 1102 32-33 0.3524829164003407 1102 34-35 0.3865873838065852 1102 36-37 0.34696970698417573 1102 38-39 0.32750987231941764 1102 40-41 0.3168935362949057 1102 42-43 0.30314695706184835 1102 44-45 0.26821099562924644 1102 46-47 0.2990553959345519 1102 48-49 0.3339129262295657 1102 50-51 0.28368142166049637 1102 52-53 0.31368713292771844 1102 54-55 0.39194168652036154 1102 56-57 0.35594626046299993 1102 58-59 0.37217714333623775 1102 60-61 0.30532584939749086 1102 62-63 0.28649683878592924 1102 64-65 0.29246348488753426 1102 66-67 0.2676042544884254 1102 68-69 0.26110512172100186 1102 70-71 0.3083817629814263 1102 72-73 0.24912600350469205 1102 74-75 0.23960452713207303 1102 76-77 0.25746980587639 1102 78-79 0.23176009140345855 1102 80-81 0.22995762193513514 1102 82-83 0.190292892139599 1102 84-85 0.18043763961417625 1102 86-87 0.2154153505619334 1102 88-89 0.1694450009545534 1102 90-91 0.1891485822093273 1102 92-93 0.19398621492284107 1102 94-95 0.16100284475553295 1102 96-97 0.1189617401026446 1102 98-99 0.10117130302169741 1102 100 0.11774022576965137 1103 1 0.22385451740699835 1103 2 0.20873803733640983 1103 3 0.23397169141902197 1103 4 0.2075534790483644 1103 5 0.1998212718355461 1103 6 0.1340680542948789 1103 7 0.1780763704723327 1103 8 0.17332392992986456 1103 9 0.23095366312814747 1103 10-11 0.24112306828445895 1103 12-13 0.24248089283032925 1103 14-15 0.27770287114698533 1103 16-17 0.2779500951397935 1103 18-19 0.33450002721589556 1103 20-21 0.28863694377290017 1103 22-23 0.2730973571195534 1103 24-25 0.27297605079849774 1103 26-27 0.2885644154312814 1103 28-29 0.27454838772767687 1103 30-31 0.24120550158718146 1103 32-33 0.2312617758894504 1103 34-35 0.2574603876617729 1103 36-37 0.23581503515035962 1103 38-39 0.23488624129775815 1103 40-41 0.21434166848161595 1103 42-43 0.2186268983031212 1103 44-45 0.2356540783597154 1103 46-47 0.30821261645483844 1103 48-49 0.26208293699598784 1103 50-51 0.20044086401192374 1103 52-53 0.30771303302029906 1103 54-55 0.3889011387539796 1103 56-57 0.3648627597890197 1103 58-59 0.3306434819686501 1103 60-61 0.27874222346217437 1103 62-63 0.2909853735356691 1103 64-65 0.2895146062641132 1103 66-67 0.32862927691802923 1103 68-69 0.2895644971727762 1103 70-71 0.27327941781253173 1103 72-73 0.2259686989504388 1103 74-75 0.24863381331838497 1103 76-77 0.23518465144234568 1103 78-79 0.23099620286421896 1103 80-81 0.25807616405871414 1103 82-83 0.22806825214358284 1103 84-85 0.20963097122628582 1103 86-87 0.21591261398231865 1103 88-89 0.1753369502792097 1103 90-91 0.16141035621053845 1103 92-93 0.1466209976708832 1103 94-95 0.15254900555748563 1103 96-97 0.10368135548746338 1103 98-99 0.07579746037496804 1103 100 0.09736257441695528 1104 1 0.196707174563457 1104 2 0.17721146065960625 1104 3 0.19275786214856083 1104 4 0.232974474145081 1104 5 0.18184052957808916 1104 6 0.14346675518702767 1104 7 0.13396863550030247 1104 8 0.16615999166553053 1104 9 0.21814132027873256 1104 10-11 0.20534228571907676 1104 12-13 0.21543667049371606 1104 14-15 0.22364186479605053 1104 16-17 0.25269172159887177 1104 18-19 0.2686325223211341 1104 20-21 0.25296675393588686 1104 22-23 0.2210462257061252 1104 24-25 0.24304140960679632 1104 26-27 0.26133328343154716 1104 28-29 0.22234900935259816 1104 30-31 0.2334432057754512 1104 32-33 0.2161653275049744 1104 34-35 0.2294001664439662 1104 36-37 0.21230860904278614 1104 38-39 0.21658313441356114 1104 40-41 0.19978186424186362 1104 42-43 0.1830867644876406 1104 44-45 0.16809108455906596 1104 46-47 0.17218911927754021 1104 48-49 0.19746148154481347 1104 50-51 0.21154908107452997 1104 52-53 0.18649325387317361 1104 54-55 0.21099328152993024 1104 56-57 0.219320300106439 1104 58-59 0.15920902120003433 1104 60-61 0.15769348379862436 1104 62-63 0.14083722542387278 1104 64-65 0.13077660294163707 1104 66-67 0.17948860991129578 1104 68-69 0.14820877871203209 1104 70-71 0.1671312347994629 1104 72-73 0.10935396706358347 1104 74-75 0.12720481339781742 1104 76-77 0.1044743836820956 1104 78-79 0.1078415862292843 1104 80-81 0.15574738523772425 1104 82-83 0.12603165308465947 1104 84-85 0.114238319562741 1104 86-87 0.10647782248178927 1104 88-89 0.09593441883408715 1104 90-91 0.08213791787347446 1104 92-93 0.0455431230621528 1104 94-95 0.011576788858619125 1104 96-97 0.025686015859971434 1104 98-99 0.02752322985729805 1104 100 0.00968987716574432 1105 1 0.13720277846486084 1105 2 0.12135862183575341 1105 3 0.14103441018355767 1105 4 0.12175321147454099 1105 5 0.10377816238528936 1105 6 0.059487107439267106 1105 7 0.06357143865383108 1105 8 0.05749061995268079 1105 9 0.0807634223935878 1105 10-11 0.08324185208483215 1105 12-13 0.0837686232459589 1105 14-15 0.10290355202874935 1105 16-17 0.11201970435680408 1105 18-19 0.17229347145309504 1105 20-21 0.15808719528821058 1105 22-23 0.13194704198133422 1105 24-25 0.1426510905570595 1105 26-27 0.16051530175607098 1105 28-29 0.13474785109927012 1105 30-31 0.1393962383589198 1105 32-33 0.1478908743875209 1105 34-35 0.11877459621694442 1105 36-37 0.09935780188907728 1105 38-39 0.1251167782129059 1105 40-41 0.10904099330776518 1105 42-43 0.133250183068931 1105 44-45 0.08269114475091044 1105 46-47 0.14135895004157106 1105 48-49 0.1253002389729474 1105 50-51 0.08159885027889402 1105 52-53 0.1071569937398138 1105 54-55 0.18562034462318167 1105 56-57 0.142825515519867 1105 58-59 0.13561220146794284 1105 60-61 0.08127910756540757 1105 62-63 0.10756495012260103 1105 64-65 0.07296208087591083 1105 66-67 0.0777361381105166 1105 68-69 0.10782988452879039 1105 70-71 0.1602004540160209 1105 72-73 0.15690504378133596 1105 74-75 0.12179703262771824 1105 76-77 0.0904346020094593 1105 78-79 0.05688917857397513 1105 80-81 0.08601403440534483 1105 82-83 0.0948286073888589 1105 84-85 0.04664975300074303 1105 86-87 0.052889822154661204 1105 88-89 0.02541344330103712 1105 90-91 0.0645160503723794 1105 92-93 0.07759771375200941 1105 94-95 0.0026458938769984286 1105 96-97 0.027108184431899218 1105 98-99 -0.034393725054822966 1105 100 -0.02962068146848651 1106 1 0.10112910228155414 1106 2 0.08734418847217995 1106 3 0.09960319421475816 1106 4 0.10215826518415128 1106 5 0.07933762007055378 1106 6 -0.006567186811572867 1106 7 0.06816820616390373 1106 8 0.08414487567902285 1106 9 0.11681089243379006 1106 10-11 0.0766182842300438 1106 12-13 0.053269104350555097 1106 14-15 0.07952732801217621 1106 16-17 0.06492577857419946 1106 18-19 0.12279616421615458 1106 20-21 0.11602673079476489 1106 22-23 0.10735273099138709 1106 24-25 0.08367101535524313 1106 26-27 0.13990825374504823 1106 28-29 0.09173381494902344 1106 30-31 0.10511975485524516 1106 32-33 0.11411626129414998 1106 34-35 0.05552560692047592 1106 36-37 0.0750626466495774 1106 38-39 0.09317479424039021 1106 40-41 0.10842745604900728 1106 42-43 0.14497704445217607 1106 44-45 0.10591594295080853 1106 46-47 0.12953884476988264 1106 48-49 0.12953707942815385 1106 50-51 0.017138917375355334 1106 52-53 0.13846963133335777 1106 54-55 0.2142065026911979 1106 56-57 0.27466117323233874 1106 58-59 0.23290920133690207 1106 60-61 0.1800261355223256 1106 62-63 0.175568694631572 1106 64-65 0.15531220209860663 1106 66-67 0.1359812369445237 1106 68-69 0.10914385557580175 1106 70-71 0.18832478667769692 1106 72-73 0.17012769708952646 1106 74-75 0.09725869431623124 1106 76-77 0.10067591606368609 1106 78-79 0.19387107657618685 1106 80-81 0.2303185772626435 1106 82-83 0.24218263939761542 1106 84-85 0.23304459463216176 1106 86-87 0.17610246383550177 1106 88-89 0.1548394907545152 1106 90-91 0.1909290048408323 1106 92-93 0.20143106804875188 1106 94-95 0.19533640102062932 1106 96-97 0.14667213348050367 1106 98-99 0.16521450006855432 1106 100 0.22493110692106555 1107 1 0.029665613118169176 1107 2 0.028985348739318795 1107 3 0.009397088411553511 1107 4 0.061313912461862685 1107 5 0.10218572230493805 1107 6 0.02473530706421201 1107 7 4.1999768215816857E-4 1107 8 -0.015845414267992908 1107 9 0.01678505808950348 1107 10-11 0.01682068768742795 1107 12-13 0.042032560007982056 1107 14-15 0.0037541122712454467 1107 16-17 0.012328453970830822 1107 18-19 0.06193934583846783 1107 20-21 0.032713720408331426 1107 22-23 0.00869204881460206 1107 24-25 -0.004402151619764538 1107 26-27 0.0025351485671052387 1107 28-29 0.03607599558911545 1107 30-31 0.001182540902192386 1107 32-33 -0.0583013763260638 1107 34-35 -0.010055211083830784 1107 36-37 -0.033450470690411294 1107 38-39 0.03034530092709531 1107 40-41 0.016229696034180563 1107 42-43 -0.029145239158573588 1107 44-45 0.006351454664802247 1107 46-47 0.03765277909257492 1107 48-49 -0.025004121868057894 1107 50-51 -0.07124149231282928 1107 52-53 0.03140355847848042 1107 54-55 0.09281936592586959 1107 56-57 0.14433973649263265 1107 58-59 0.12684158606578677 1107 60-61 0.1351961745513819 1107 62-63 0.10024768100678472 1107 64-65 0.12154145461067856 1107 66-67 0.1799645077473926 1107 68-69 0.1437208465117834 1107 70-71 0.12991181500005666 1107 72-73 0.08705297918254473 1107 74-75 0.10983505724463782 1107 76-77 0.11665693702354929 1107 78-79 0.14771285210763097 1107 80-81 0.13254349213793404 1107 82-83 0.10176667988212884 1107 84-85 0.13939396294509976 1107 86-87 0.1304815007240805 1107 88-89 0.049807526214198816 1107 90-91 0.06502678550282326 1107 92-93 0.10746256884029037 1107 94-95 0.10774908028467678 1107 96-97 0.08750116654951867 1107 98-99 0.05506182564792894 1107 100 0.05771795311939698 1108 1 -0.014799204219883677 1108 2 -0.024403621814329313 1108 3 0.02227999816204118 1108 4 0.06819379561606809 1108 5 0.04434355989070582 1108 6 0.016364697026382657 1108 7 -0.00652395392703653 1108 8 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54-55 -1.0895994796389985 2316 56-57 -1.4447475321988676 2316 58-59 -1.3119354895869222 2316 60-61 -1.1743186450216996 2316 62-63 -1.156557783008843 2316 64-65 -1.0020304042338068 2316 66-67 -0.7671403905459186 2316 68-69 -0.8555905641127737 2316 70-71 -0.8038878401109883 2316 72-73 -0.8878614603646255 2316 74-75 -0.832367622758369 2316 76-77 -0.925051116500228 2316 78-79 -0.8162662167407468 2316 80-81 -0.7895598095107772 2316 82-83 -0.8537483462250712 2316 84-85 -1.0364247843893253 2316 86-87 -1.0297739927925669 2316 88-89 -0.8183771935196908 2316 90-91 -0.8761195012759018 2316 92-93 -0.9688774865537546 2316 94-95 -1.0749686345260976 2316 96-97 -0.8502108286532746 2316 98-99 -0.932405162251424 2316 100 -1.0888828245938988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72.0 3 167.0 4 94.0 5 59.0 6 249.0 7 2598.0 8 8066.0 9 14000.0 10 18020.0 11 20373.0 12 24322.0 13 30324.0 14 39792.0 15 52899.0 16 68170.0 17 86033.0 18 96339.0 19 109239.0 20 125422.0 21 153900.0 22 205575.0 23 304829.0 24 466020.0 25 616363.0 26 623559.0 27 503946.0 28 402975.0 29 390799.0 30 434758.0 31 520488.0 32 644407.0 33 830761.0 34 1152803.0 35 1915033.0 36 3062886.0 37 5578585.0 38 7837400.0 39 2629228.0 40 4787.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.515308950110594 38.80386275044293 26.976586498375816 17.70424180107066 2 19.355495219877096 36.68021947382014 26.554143475506947 17.410141830795823 3 27.390118014301184 26.688437125632053 26.66990148094136 19.2515433791254 4 18.554553907125047 27.292028804448048 26.097014687849285 28.056402600577616 5 19.166652456671194 35.76406639397743 26.207493502465184 18.861787646886192 6 28.227521751695424 26.81846445908867 26.354453383018278 18.59956040619763 7 19.22074172759139 26.792568534335864 35.24414259366331 18.742547144409432 8 27.916076328215773 27.111746589649776 26.213057056434224 18.75912002570022 9 27.477873082402837 26.90086133108026 26.81314748241167 18.808118104105233 10-11 18.575402771039972 31.853669794283007 30.949554482887326 18.62137295178969 12-13 18.562660048618483 27.384121119822268 30.72388966382837 23.329329167730876 14-15 18.58584086495983 36.22177481555695 26.331795878513642 18.860588440969583 16-17 23.26863391434823 27.10206326726829 26.258381715752904 23.37092110263058 18-19 18.989631618821978 31.736568086705695 26.292639170945293 22.981161123527034 20-21 23.242740809477684 31.831298021423976 26.286269668413574 18.639691500684762 22-23 23.089427209234355 27.48993709518318 30.74322085690438 18.677414838678086 24-25 18.683346046850858 31.56160454768422 26.399644885626927 23.355404519837997 26-27 18.64510494896402 27.34441731876708 35.32794479089797 18.68253294137093 28-29 18.72752076213319 27.33666718050083 35.30057262019595 18.635239437170036 30-31 18.742951112694545 27.266740345305674 35.32218429488709 18.668124247112694 32-33 18.76207783929597 27.32211447478292 35.23803247029881 18.677775215622297 34-35 18.784200490694403 27.466045508823644 35.106253844443536 18.643500156038417 36-37 18.710392092014086 27.45127143960359 35.15337199491075 18.684964473471577 38-39 18.76588166111611 27.407616031576836 35.08478264632684 18.741719660980213 40-41 18.85494414375568 27.463212929997677 34.985852434609036 18.695990491637605 42-43 18.860856899389937 27.522995976065523 34.994306414101544 18.621840710442996 44-45 18.986568321795637 27.580811257176745 34.82129074882152 18.6113296722061 46-47 18.862416436067523 27.670209710448372 34.80797370876385 18.659400144720248 48-49 19.00662677237576 27.680806719259838 34.607995951231864 18.70457055713254 50-51 19.057502436279215 27.775790146441466 34.46711839708712 18.6995890201922 52-53 19.13599562269405 28.022086841060485 34.18788044458964 18.65403709165583 54-55 19.553513928779243 28.289299215951814 33.25683013715839 18.900356718110555 56-57 20.092061675748905 29.048189539129922 31.8296052257499 19.030143559371275 58-59 20.305932427230577 29.606893631569314 31.05972707157082 19.027446869629287 60-61 20.394467270058207 29.980186004875986 30.625153138270473 19.000193586795337 62-63 20.473903764368156 30.16559190520585 30.413293128078855 18.947211202347138 64-65 20.628282575136787 30.226703686017242 30.32443681482984 18.820576924016134 66-67 20.751687174529625 30.022563305617716 30.51058389751376 18.715165622338898 68-69 20.85985918010923 29.722160406233204 30.81283258976982 18.605147823887748 70-71 21.010778455926935 29.521020883824583 30.995491842494644 18.472708817753837 72-73 21.171885094862024 29.41315788819766 30.97127309428332 18.443683922656998 74-75 21.380178484660874 29.476510752264932 30.76223140303911 18.381079360035084 76-77 21.674578993469062 29.65679665941632 30.333205389423373 18.335418957691243 78-79 21.98995957894875 29.872829904140318 29.867387165196707 18.269823351714226 80-81 22.332186668330277 30.029338367649157 29.406520398496504 18.231954565524067 82-83 22.51480592684045 30.21139761477781 29.028305799867788 18.245490658513955 84-85 22.715390956933174 30.204263454782172 28.807734085768004 18.27261150251665 86-87 22.935113955285974 30.173064495487612 28.650852992455718 18.2409685567707 88-89 23.15224433627904 30.130099035683166 28.518413030421495 18.199243597616295 90-91 23.418592033839342 30.051155220303606 28.328847789233038 18.201404956624017 92-93 23.64016668891894 29.988182914111434 28.220260306524292 18.15139009044534 94-95 23.826851919919626 29.946378508745138 28.109506360617083 18.117263210718153 96-97 23.95629668831641 29.93080667346671 27.971349531996015 18.141547106220866 98-99 24.088054633153124 29.942524106884168 27.83031165439353 18.13910960556918 100 24.1584281309016 30.01302902431154 27.681828789432195 18.146714055354664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242447.0 1 141558.0 2 31574.5 3 20683.5 4 18425.0 5 18135.5 6 18315.5 7 17964.0 8 17376.0 9 17279.5 10 18463.5 11 28484.5 12 275700.0 13 318023.5 14 111251.5 15 105405.0 16 116891.5 17 128675.0 18 139196.0 19 150170.5 20 165330.5 21 188778.5 22 219714.0 23 252390.5 24 284021.5 25 317797.0 26 354481.5 27 399485.5 28 467840.5 29 537248.0 30 585663.0 31 636675.5 32 692659.0 33 731105.5 34 756220.5 35 799289.5 36 847667.0 37 873426.5 38 902160.5 39 921731.0 40 916344.5 41 924777.5 42 955705.0 43 954437.5 44 928222.0 45 918820.0 46 911032.0 47 900830.5 48 875872.5 49 843879.0 50 819342.0 51 793208.5 52 757984.5 53 703808.0 54 636832.5 55 569982.5 56 508881.5 57 452405.0 58 395515.5 59 333125.0 60 270047.5 61 217330.0 62 169913.5 63 124276.5 64 88078.0 65 62525.5 66 44445.5 67 33310.5 68 25475.5 69 18739.0 70 13980.0 71 11141.0 72 9364.5 73 7999.5 74 7353.5 75 7242.0 76 5619.5 77 2882.0 78 1445.5 79 1024.0 80 746.0 81 574.0 82 438.5 83 342.0 84 295.0 85 240.0 86 181.0 87 153.5 88 130.0 89 109.5 90 91.5 91 81.5 92 64.5 93 50.5 94 48.0 95 41.5 96 28.5 97 18.5 98 13.0 99 8.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004741963338480239 2 1.3459721266428625E-4 3 0.08874097767273827 4 0.025242154190425373 5 0.01767710059657626 6 0.05636862242168686 7 0.037172989169410954 8 0.025863372095029775 9 0.00545291271819416 10-11 0.0076289009896001216 12-13 0.014703882680927989 14-15 0.02643454744620771 16-17 0.04912798262246448 18-19 0.06253421012488551 20-21 0.04511249911131328 22-23 0.02652427892131723 24-25 0.019045505591996505 26-27 0.017409631776538256 28-29 0.012445065355574776 30-31 0.010786758671339146 32-33 0.00807238154927604 34-35 0.005887765251417239 36-37 0.010029217948779893 38-39 0.014593443942331651 40-41 0.015982556201238708 42-43 0.009751395496998482 44-45 0.0038394717715132935 46-47 0.0028230902553688757 48-49 0.0020741775592624627 50-51 0.0010888569383482643 52-53 0.0015202582609902076 54-55 2.760968464908436E-4 56-57 0.002562523856493142 58-59 0.010672868722161673 60-61 0.022163674352052472 62-63 0.027547562858623916 64-65 0.008850629535322105 66-67 0.0022105003772173166 68-69 0.0015737520249978086 70-71 0.0022174027983795875 72-73 7.040469585516511E-4 74-75 6.52278799834618E-4 76-77 0.0013925634694881923 78-79 0.0033045341314372843 80-81 0.007549523146234004 82-83 0.008860983167065512 84-85 0.007570230409720818 86-87 0.004638427021046172 88-89 0.0023554512216250097 90-91 0.002691944253285725 92-93 0.002652255331602666 94-95 0.003278650052078768 96-97 0.0034995275292714428 98-99 0.007718632464709647 100 0.013352733738413423 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.897534E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.01093191273261 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37153342099462 28.26581623515415 2 11.382468244586638 8.425515143984743 3 4.104457245039201 4.557293629046037 4 2.088061444463787 3.091243998026053 5 1.2632717897911832 2.3377433099618665 6 0.846406524161882 1.879577654174885 7 0.6022725155195393 1.5603466945362707 8 0.47228284576619095 1.398370259856327 9 0.3484900916558985 1.1608148749084566 >10 2.089869081889175 14.224962290040233 >50 0.2148581847031952 5.635579459981893 >100 0.21019155814823 16.462198658897282 >500 0.004888715672481667 1.0598519788173013 >1k 7.254326797179395E-4 0.6480044086255582 >5k 1.393155802269121E-4 0.3406116610049675 >10k+ 8.358934813107117E-5 8.95206974298394 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2146011 7.406335870433272 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 247172 0.8530426217604349 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73698 0.2543473174085274 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 43079 0.14867470062473814 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 38044 0.13129785534872065 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.15343392001612405 0.0 0.0 3.451210581135545E-6 6.90242116227109E-6 2 0.15345462727961087 1.0353631743406635E-5 0.0 3.451210581135545E-6 1.7256052905677725E-5 3 0.15345462727961087 1.0353631743406635E-5 0.0 3.451210581135545E-6 2.070726348681327E-5 4 0.15346152970077315 2.4158474067948815E-5 0.0 3.451210581135545E-6 2.4158474067948815E-5 5 0.15346843212193542 2.4158474067948815E-5 0.0 3.451210581135545E-6 2.4158474067948815E-5 6 0.1534960418065845 2.760968464908436E-5 0.0 6.90242116227109E-6 2.4158474067948815E-5 7 0.1535063954383279 3.451210581135545E-5 0.0 6.90242116227109E-6 2.4158474067948815E-5 8 0.1535063954383279 3.451210581135545E-5 0.0 6.90242116227109E-6 2.4158474067948815E-5 9 0.15350984664890904 3.796331639249099E-5 0.0 6.90242116227109E-6 2.4158474067948815E-5 10-11 0.15350984664890904 4.314013226419431E-5 0.0 8.628026452838863E-6 3.623771110192322E-5 12-13 0.15351502346478074 4.831694813589763E-5 0.0 1.380484232454218E-5 3.796331639249099E-5 14-15 0.15352537709652414 5.176815871703317E-5 0.0 1.7256052905677725E-5 4.141452697362654E-5 16-17 0.153534005122977 5.521936929816872E-5 0.0 2.070726348681327E-5 4.141452697362654E-5 18-19 0.15354608436001096 6.557300104157536E-5 0.0 2.070726348681327E-5 4.831694813589763E-5 20-21 0.15355643799175436 7.074981691327866E-5 0.0 2.070726348681327E-5 5.349376400760094E-5 22-23 0.15360475493989026 8.455465923782086E-5 0.0 2.243286877738104E-5 5.694497458873649E-5 24-25 0.15379629712714327 9.835950156236302E-5 0.0 2.4158474067948815E-5 7.074981691327866E-5 26-27 0.15471431914172534 1.0353631743406636E-4 0.0 2.4158474067948815E-5 7.247542220384644E-5 28-29 0.15802920690490604 1.190667650491763E-4 0.0 2.4158474067948815E-5 7.937784336611752E-5 30-31 0.16700408002114903 1.2079237033974408E-4 0.0 2.4158474067948815E-5 8.973147510952416E-5 32-33 0.186035780770821 1.328716073737185E-4 0.0 2.760968464908436E-5 9.145708040009194E-5 34-35 0.22088610521912772 1.4149963382655733E-4 0.0 2.760968464908436E-5 9.663389627179526E-5 36-37 0.277265081272558 1.622068973133706E-4 0.0 2.760968464908436E-5 1.000851068529308E-4 38-39 0.3623615805716171 1.6738371318507394E-4 0.0 2.760968464908436E-5 1.000851068529308E-4 40-41 0.47765789806090286 1.760117396379128E-4 0.0 2.933528993965213E-5 1.0181071214349858E-4 42-43 0.62745424212451 1.8636537138131944E-4 0.0 3.278650052078768E-5 1.0526192272463412E-4 44-45 0.8115970338915781 1.8981658196245497E-4 0.0 4.141452697362654E-5 1.1043873859633744E-4 46-47 1.038303605755791 1.9671900312472605E-4 0.0 4.486573755476208E-5 1.2079237033974408E-4 48-49 1.3091477097421462 2.0189581899642938E-4 0.0 4.486573755476208E-5 1.2079237033974408E-4 50-51 1.6241759371934892 2.1224945073983602E-4 0.0 4.831694813589763E-5 1.242435809208796E-4 52-53 1.9788326901427213 2.139750560304038E-4 0.0 4.831694813589763E-5 1.242435809208796E-4 54-55 2.370384609809583 2.243286877738104E-4 0.0 5.349376400760094E-5 1.242435809208796E-4 56-57 2.8032941114754824 2.2777989835494596E-4 0.0 5.521936929816872E-5 1.242435809208796E-4 58-59 3.28005642039058 2.3123110893608152E-4 0.0 5.521936929816872E-5 1.242435809208796E-4 60-61 3.7931979400414284 2.4331034597005593E-4 0.0 5.521936929816872E-5 1.2769479150201516E-4 62-63 4.338187230934995 2.553895830040303E-4 0.0 5.521936929816872E-5 1.3459721266428625E-4 64-65 4.909945836701139 2.588407935851659E-4 0.0 5.521936929816872E-5 1.3632281795485402E-4 66-67 5.514342540933082 2.6229200416630143E-4 0.0 5.521936929816872E-5 1.380484232454218E-4 68-69 6.151106423600206 2.6229200416630143E-4 0.0 5.521936929816872E-5 1.432252391171251E-4 70-71 6.814862224222391 2.7264563590970805E-4 0.0 5.867057987930426E-5 1.4495084440769288E-4 72-73 7.502785126938976 3.0198092584936016E-4 0.0 5.867057987930426E-5 1.5185326556996397E-4 74-75 8.209227225633935 3.0370653113992793E-4 0.0 6.21217904604398E-5 1.5357887086053174E-4 76-77 8.938259223187718 3.071577417210635E-4 0.0 6.21217904604398E-5 1.5530447615109952E-4 78-79 9.686938962579905 3.123345575927668E-4 0.0 6.384739575100758E-5 1.5530447615109952E-4 80-81 10.45728540200046 3.2786500520787677E-4 0.0 6.557300104157536E-5 1.5530447615109952E-4 82-83 11.246069933950732 3.4684666340412226E-4 0.0 6.557300104157536E-5 1.6048129202280283E-4 84-85 12.046952684593174 3.554746898569611E-4 0.0 6.557300104157536E-5 1.6738371318507394E-4 86-87 12.860625621649305 3.692795321815033E-4 0.0 6.729860633214314E-5 1.691093184756417E-4 88 13.481674416935228 3.727307427626389E-4 0.0 7.247542220384644E-5 1.7256052905677724E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 273425 0.0 87.333984 8 AGTGGTA 275525 0.0 87.18295 5 AAGCAGT 281025 0.0 87.07828 1 TGGTATC 274905 0.0 86.64199 7 GTGGTAT 276345 0.0 86.61818 6 GCAGTGG 283480 0.0 86.00791 3 GTATCAA 277520 0.0 85.26852 9 AGCAGTG 292780 0.0 83.87387 2 CAGTGGT 289795 0.0 83.360146 4 CAACGCA 270950 0.0 45.659935 12-13 ATCAACG 271365 0.0 45.578224 10-11 ACGCAGA 275995 0.0 45.137714 14-15 AGAGTAC 277610 0.0 44.643898 18-19 TCAACGC 271115 0.0 43.60573 12-13 AACGCAG 273080 0.0 43.119556 14-15 AGTACTT 285740 0.0 43.11934 20-21 TATCAAC 274055 0.0 43.081047 10-11 CGCAGAG 276650 0.0 42.437286 16-17 GAGTACT 274995 0.0 41.940067 20-21 GCAGAGT 297050 0.0 41.756245 16-17 >>END_MODULE