FastQCFastQC Report
Thu 26 May 2016
SRR1660694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28975340
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16683385.757785758510512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1784630.6159133939411927No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010850.3488656215940866No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT475870.16423275792449718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2160300.084.873828
AAGCAGT2220400.084.371661
AGTGGTA2194150.084.3309865
TGGTATC2184950.083.901377
GTGGTAT2199850.083.7505956
GCAGTGG2258400.083.015043
GTATCAA2204750.082.386749
AGCAGTG2353350.080.1985862
CAGTGGT2336850.079.7792364
CAACGCA2127300.044.92200512-13
ATCAACG2125450.044.81083710-11
ACGCAGA2177250.044.3132814-15
AGAGTAC2213750.043.62285218-19
TCAACGC2127300.042.49464812-13
AACGCAG2146450.041.90916414-15
AGTACTT2298600.041.87296320-21
TATCAAC2170950.041.70466210-11
CGCAGAG2182550.041.21703716-17
GAGTACT2188000.040.74818420-21
GCAGAGT2409450.040.1617216-17
Produced by FastQC (version 0.11.5)