##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660693_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33262892 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1142086202246 33.0 31.0 34.0 30.0 34.0 2 32.26930117802144 34.0 31.0 34.0 30.0 34.0 3 32.221806600580614 34.0 31.0 34.0 30.0 34.0 4 35.63194787753272 37.0 35.0 37.0 35.0 37.0 5 35.672818106134606 37.0 35.0 37.0 35.0 37.0 6 35.662597136773314 37.0 35.0 37.0 35.0 37.0 7 35.6062445201698 37.0 35.0 37.0 33.0 37.0 8 35.687002501165566 37.0 35.0 37.0 35.0 37.0 9 37.371937954162256 39.0 37.0 39.0 35.0 39.0 10-11 37.29119590683817 39.0 37.0 39.0 34.0 39.0 12-13 37.327991925656974 39.0 37.0 39.0 34.0 39.0 14-15 38.7041437046424 40.0 38.0 41.0 35.0 41.0 16-17 38.609469134553905 40.0 38.0 41.0 34.0 41.0 18-19 38.4652052202797 40.0 38.0 41.0 34.0 41.0 20-21 38.345622954251844 40.0 38.0 41.0 34.0 41.0 22-23 38.13536837085603 40.0 38.0 41.0 33.5 41.0 24-25 38.145080920805086 40.0 38.0 41.0 34.0 41.0 26-27 38.057526206681004 40.0 38.0 41.0 33.5 41.0 28-29 37.91758069322415 40.0 38.0 41.0 33.0 41.0 30-31 37.72022017808915 40.0 37.0 41.0 32.5 41.0 32-33 37.5485595780427 40.0 37.0 41.0 32.0 41.0 34-35 37.40863234321297 40.0 37.0 41.0 31.5 41.0 36-37 37.15144791078298 39.5 37.0 41.0 31.0 41.0 38-39 36.75758716349739 39.0 35.0 41.0 30.5 41.0 40-41 36.81628452510984 39.0 35.0 41.0 31.0 41.0 42-43 36.60883733440856 39.0 35.0 41.0 30.0 41.0 44-45 36.44158532577384 39.0 35.0 41.0 30.0 41.0 46-47 36.46294083509035 39.0 35.0 41.0 30.0 41.0 48-49 36.401971241706825 39.0 35.0 41.0 29.5 41.0 50-51 34.95673460984691 38.0 34.0 39.5 26.0 40.0 52-53 34.94387416764604 37.5 33.5 39.5 26.0 40.0 54-55 35.31333699126341 39.0 35.0 40.5 24.0 41.0 56-57 34.81063259622765 39.0 34.0 41.0 19.5 41.0 58-59 34.46434170546566 39.0 34.0 41.0 18.0 41.0 60-61 34.17709867199761 38.5 34.0 40.0 16.0 41.0 62-63 33.782863272983 37.5 33.0 40.0 15.0 41.0 64-65 33.29095422610878 37.0 32.5 39.5 13.5 41.0 66-67 32.7330676478762 36.0 32.0 39.0 11.5 41.0 68-69 32.26903719015172 35.5 31.5 39.0 10.0 40.0 70-71 31.779119401283566 35.0 31.0 37.5 10.0 39.0 72-73 31.367332657064217 35.0 31.0 37.0 10.0 39.0 74-75 30.977631199355727 35.0 30.0 36.5 10.0 38.5 76-77 30.545445071342563 34.5 30.0 35.5 10.0 37.0 78-79 30.240394340937044 34.0 29.0 35.0 10.0 37.0 80-81 29.96478933641729 34.0 29.0 35.0 10.0 36.5 82-83 29.719221828336515 34.0 29.0 35.0 10.0 36.0 84-85 29.485645054555086 34.0 29.0 35.0 10.0 36.0 86-87 29.283461296750744 34.0 28.5 35.0 9.0 35.0 88-89 29.096248456087345 34.0 27.5 35.0 8.0 35.0 90-91 28.914282934268012 34.0 27.0 35.0 8.0 35.0 92-93 28.72929536613954 34.0 27.0 35.0 7.0 35.0 94-95 28.60802351461202 34.0 26.5 35.0 7.0 35.0 96-97 28.46830924983913 34.0 25.5 35.0 7.0 35.0 98-99 28.271313961516036 34.0 25.0 35.0 7.0 35.0 100 28.16189518337732 33.0 25.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2829636690446904 1101 2 0.2772563024341821 1101 3 0.26403964428556037 1101 4 0.20392085139356197 1101 5 0.21908650562306775 1101 6 0.15917460253920268 1101 7 0.19170090357922476 1101 8 0.19839748280232072 1101 9 0.2830574106488015 1101 10-11 0.27407609449121395 1101 12-13 0.27494540354586405 1101 14-15 0.318646767931277 1101 16-17 0.29016850516067905 1101 18-19 0.37535541068386635 1101 20-21 0.32036624017356274 1101 22-23 0.29549197606373667 1101 24-25 0.3114922282569452 1101 26-27 0.32224289437578335 1101 28-29 0.3261477134602089 1101 30-31 0.34257305478029565 1101 32-33 0.33671101168167183 1101 34-35 0.3417135716076345 1101 36-37 0.31410597407248275 1101 38-39 0.306473327353352 1101 40-41 0.28309311062046305 1101 42-43 0.3006898766956141 1101 44-45 0.2679305357499828 1101 46-47 0.3143064069238406 1101 48-49 0.3269512865487627 1101 50-51 0.27326057673186455 1101 52-53 0.3626735066372433 1101 54-55 0.40120146397264733 1101 56-57 0.46112477976692645 1101 58-59 0.4410887136488668 1101 60-61 0.40210348783615757 1101 62-63 0.36112430140067886 1101 64-65 0.3657134833717066 1101 66-67 0.387442740954711 1101 68-69 0.33648202248467385 1101 70-71 0.3272850125380522 1101 72-73 0.27901103534168215 1101 74-75 0.25466574446561907 1101 76-77 0.2755117845585495 1101 78-79 0.3230357312593668 1101 80-81 0.2766684186741841 1101 82-83 0.25089635247555364 1101 84-85 0.29389839621000036 1101 86-87 0.3076742467527005 1101 88-89 0.2553491288052001 1101 90-91 0.24191027797260034 1101 92-93 0.24843101705323178 1101 94-95 0.23792884159400884 1101 96-97 0.19356204675763777 1101 98-99 0.16159582848036536 1101 100 0.14747994408271126 1102 1 0.2533332707342879 1102 2 0.25987203360329403 1102 3 0.26569219716393633 1102 4 0.2219621800919498 1102 5 0.199083239823878 1102 6 0.13216814477462435 1102 7 0.14651027946437978 1102 8 0.156309936003602 1102 9 0.24440552673538463 1102 10-11 0.22581683323468837 1102 12-13 0.219408917064996 1102 14-15 0.24570845754718817 1102 16-17 0.2668733613486438 1102 18-19 0.30047031008339786 1102 20-21 0.2598372417862507 1102 22-23 0.21670425197620347 1102 24-25 0.22732825678273372 1102 26-27 0.2637469487305424 1102 28-29 0.24761364936196628 1102 30-31 0.30564778293311434 1102 32-33 0.2898461955764233 1102 34-35 0.30515490005753776 1102 36-37 0.2756690610659689 1102 38-39 0.2744892865948998 1102 40-41 0.23219923066595527 1102 42-43 0.21808883337327245 1102 44-45 0.2017012121024493 1102 46-47 0.2077743192488768 1102 48-49 0.22760144146230488 1102 50-51 0.19784852262907293 1102 52-53 0.24663903243694563 1102 54-55 0.29343901333800204 1102 56-57 0.2856027947267279 1102 58-59 0.2695943753319625 1102 60-61 0.2283307487410795 1102 62-63 0.20247052719560088 1102 64-65 0.23495542798131197 1102 66-67 0.2348786262182614 1102 68-69 0.19966607656399304 1102 70-71 0.24705574161377442 1102 72-73 0.1534265783258526 1102 74-75 0.16713389256830524 1102 76-77 0.1618399835109514 1102 78-79 0.13974518273914782 1102 80-81 0.1967862676593093 1102 82-83 0.1720020821278503 1102 84-85 0.1273598529612947 1102 86-87 0.1754810175787398 1102 88-89 0.1475831430742076 1102 90-91 0.14638894655165657 1102 92-93 0.10885257580439855 1102 94-95 0.09538744930834753 1102 96-97 0.09387412666855255 1102 98-99 0.06697856661268631 1102 100 0.08327278384552628 1103 1 0.19907729618158498 1103 2 0.17656623182526943 1103 3 0.1964226491306107 1103 4 0.18149597705730258 1103 5 0.15674979224635877 1103 6 0.1006974900960671 1103 7 0.11745735330933371 1103 8 0.12933233102907593 1103 9 0.1908609946662736 1103 10-11 0.18173247932679004 1103 12-13 0.1919237920757766 1103 14-15 0.22535689398190328 1103 16-17 0.24493606103905563 1103 18-19 0.2966388690994677 1103 20-21 0.2328283792038235 1103 22-23 0.24127629939241046 1103 24-25 0.2100648492856223 1103 26-27 0.22791358407784656 1103 28-29 0.25338693163291026 1103 30-31 0.23431450334476267 1103 32-33 0.21252141180148953 1103 34-35 0.23573198752520597 1103 36-37 0.19761232124000117 1103 38-39 0.17230236456874337 1103 40-41 0.14346653157799238 1103 42-43 0.09246853824739532 1103 44-45 0.1625024242848241 1103 46-47 0.22330375592718354 1103 48-49 0.1987791108729695 1103 50-51 0.14308085588942276 1103 52-53 0.22883167275505656 1103 54-55 0.2737192824009682 1103 56-57 0.23825320844471065 1103 58-59 0.22438038836281748 1103 60-61 0.19596247303805114 1103 62-63 0.17458255528004685 1103 64-65 0.14600134242223106 1103 66-67 0.15783806180540694 1103 68-69 0.17538732751443575 1103 70-71 0.19703186028329256 1103 72-73 0.09366225618277113 1103 74-75 0.15893467506920445 1103 76-77 0.10799467696529419 1103 78-79 0.09870616321941839 1103 80-81 0.14922419271394816 1103 82-83 0.13625463669566784 1103 84-85 0.16325012966750663 1103 86-87 0.16921765025737656 1103 88-89 0.1171505183485948 1103 90-91 0.1546658062947941 1103 92-93 0.17180907781549948 1103 94-95 0.12944088129862408 1103 96-97 0.11522223192931236 1103 98-99 0.1065591366489862 1103 100 0.07415756430135545 1104 1 0.19671973659952613 1104 2 0.18208574850402925 1104 3 0.19025632350997057 1104 4 0.21882592339161988 1104 5 0.17878897497002555 1104 6 0.1430396636911908 1104 7 0.12235028189899566 1104 8 0.1552438757359269 1104 9 0.19117644978040005 1104 10-11 0.20478259786262498 1104 12-13 0.21618990385724857 1104 14-15 0.24882911001129315 1104 16-17 0.235938291703242 1104 18-19 0.2786830281586532 1104 20-21 0.23249152704822507 1104 22-23 0.22134660290455344 1104 24-25 0.22578055275499054 1104 26-27 0.23725704824082783 1104 28-29 0.2210317075102992 1104 30-31 0.22768351669804332 1104 32-33 0.21827362634786596 1104 34-35 0.22130716149627006 1104 36-37 0.18550214570748125 1104 38-39 0.23383614572815503 1104 40-41 0.21382843729389123 1104 42-43 0.19594454960549967 1104 44-45 0.13256885712241484 1104 46-47 0.1647714371572775 1104 48-49 0.2021166847135305 1104 50-51 0.18008496525506246 1104 52-53 0.2350064164638681 1104 54-55 0.26249760241929465 1104 56-57 0.30429813813749007 1104 58-59 0.2843031981623483 1104 60-61 0.2770924668550663 1104 62-63 0.2689150719932414 1104 64-65 0.29384942084647037 1104 66-67 0.25074495062329305 1104 68-69 0.2487380938317756 1104 70-71 0.2578921671369052 1104 72-73 0.17352142172566687 1104 74-75 0.20861953029884006 1104 76-77 0.2209489481792879 1104 78-79 0.20114856906374 1104 80-81 0.2693736870374046 1104 82-83 0.26830855801195597 1104 84-85 0.29212437638677713 1104 86-87 0.3007315189238362 1104 88-89 0.261172140485769 1104 90-91 0.21714773288567457 1104 92-93 0.18472552524306707 1104 94-95 0.18106995898783396 1104 96-97 0.23587561028289272 1104 98-99 0.22818294697500718 1104 100 0.13775826816405967 1105 1 0.1344860185443224 1105 2 0.12190620734434532 1105 3 0.1559950441867386 1105 4 0.14828777144455074 1105 5 0.12824206943100336 1105 6 0.0722380005490848 1105 7 0.07881894532160061 1105 8 0.0874704591283475 1105 9 0.098972890732405 1105 10-11 0.11408628795214781 1105 12-13 0.12947717023050132 1105 14-15 0.1447365012337869 1105 16-17 0.12377511060749669 1105 18-19 0.17763922358446393 1105 20-21 0.1500345177711111 1105 22-23 0.1372826178830806 1105 24-25 0.11043599648235158 1105 26-27 0.12429526949079417 1105 28-29 0.12742898863232455 1105 30-31 0.12500554307844425 1105 32-33 0.15783447149068763 1105 34-35 0.12319663813629944 1105 36-37 0.12754602140692128 1105 38-39 0.0797864520270295 1105 40-41 0.06604507063192244 1105 42-43 0.08354347388231531 1105 44-45 0.0655648048991111 1105 46-47 0.14149058102595546 1105 48-49 0.10432319332324624 1105 50-51 0.055483176574398385 1105 52-53 0.11307745342415387 1105 54-55 0.2104594601996581 1105 56-57 0.16309853817239883 1105 58-59 0.17366081469648975 1105 60-61 0.11826196038431647 1105 62-63 0.14499652207219782 1105 64-65 0.09885149987194808 1105 66-67 0.11549629788341775 1105 68-69 0.10127175200495486 1105 70-71 0.1496283533435836 1105 72-73 0.12471759348290234 1105 74-75 0.1599994292137339 1105 76-77 0.0821570293599585 1105 78-79 0.10476734722158199 1105 80-81 0.10231065468908085 1105 82-83 0.11080379796885254 1105 84-85 0.08692163626988503 1105 86-87 0.06107156308884143 1105 88-89 0.016512228864542067 1105 90-91 0.05368168512014648 1105 92-93 0.07221574511305207 1105 94-95 0.019300333719911578 1105 96-97 0.016062802503402196 1105 98-99 0.01746139161533833 1105 100 0.007511399691221499 1106 1 0.11398357616363342 1106 2 0.10899034878405445 1106 3 0.1334873600109816 1106 4 0.12100606716291651 1106 5 0.11860129748221482 1106 6 0.058764321279156206 1106 7 0.08281265780900071 1106 8 0.10826137349323517 1106 9 0.13545248629540652 1106 10-11 0.11144649355153291 1106 12-13 0.09713977180077649 1106 14-15 0.12068142550415217 1106 16-17 0.10305468466074075 1106 18-19 0.14794487967046166 1106 20-21 0.14652892418531138 1106 22-23 0.15875159014728268 1106 24-25 0.12432969388515858 1106 26-27 0.16460619531762433 1106 28-29 0.1001359835455915 1106 30-31 0.12349782209413007 1106 32-33 0.15596378127042243 1106 34-35 0.06115054927156649 1106 36-37 0.05884980707705978 1106 38-39 0.057773021352311105 1106 40-41 0.08900051773370876 1106 42-43 0.10179136751244755 1106 44-45 0.10106404206911179 1106 46-47 0.07782555793982482 1106 48-49 0.09396094787409481 1106 50-51 -0.04416776797918942 1106 52-53 0.046233689073581274 1106 54-55 0.08859743047976565 1106 56-57 0.1279249129739526 1106 58-59 0.11105572904410366 1106 60-61 0.0761648536006021 1106 62-63 0.06862288484553147 1106 64-65 0.026742393955629495 1106 66-67 0.0616482291803635 1106 68-69 0.06888428872070307 1106 70-71 0.12802344718123138 1106 72-73 0.09009781613348267 1106 74-75 0.06474211739257996 1106 76-77 0.028466487002074103 1106 78-79 0.04501576785936123 1106 80-81 0.1010470898030853 1106 82-83 0.11563908780629006 1106 84-85 0.12616529096278484 1106 86-87 0.06495939881713397 1106 88-89 0.035578351434544686 1106 90-91 0.059401588992827214 1106 92-93 0.0581802804267717 1106 94-95 0.08453365827880077 1106 96-97 0.03951141768022026 1106 98-99 0.017745356419634817 1106 100 0.03152909993011832 1107 1 0.03709599552634302 1107 2 0.0635466359347916 1107 3 0.0718424267613571 1107 4 0.10215862258426966 1107 5 0.0880630046753268 1107 6 0.025111639880044834 1107 7 0.0023686392971242753 1107 8 0.008519212030932977 1107 9 0.04173293649184018 1107 10-11 0.05215124702329632 1107 12-13 0.06827946058940171 1107 14-15 0.007544720857772802 1107 16-17 0.002946907686919076 1107 18-19 0.08659997552678078 1107 20-21 0.04997431587887036 1107 22-23 0.05230224329548605 1107 24-25 0.025092102082432177 1107 26-27 0.014643670806655962 1107 28-29 0.058450542213641654 1107 30-31 -0.0024104541795466616 1107 32-33 -0.033317256543988094 1107 34-35 0.0105580676124859 1107 36-37 -0.025189238612924214 1107 38-39 -0.0038123162864209803 1107 40-41 -0.033418565523994914 1107 42-43 -0.07098644863081915 1107 44-45 -0.03209266418134149 1107 46-47 -0.037356236237862106 1107 48-49 -0.06817570615186952 1107 50-51 -0.1485091953384483 1107 52-53 -0.04993864534944947 1107 54-55 -0.03357484873183125 1107 56-57 -0.032080101920179516 1107 58-59 0.005319616064596744 1107 60-61 -0.03850265685908738 1107 62-63 -0.06067847076141675 1107 64-65 -0.08111806986002534 1107 66-67 -0.045208823308385604 1107 68-69 -0.036047647444824804 1107 70-71 -0.0340450139710633 1107 72-73 -0.08404711293102451 1107 74-75 -0.06949983126625625 1107 76-77 -0.0750287380864414 1107 78-79 -0.04297801182266525 1107 80-81 -0.025804460070578017 1107 82-83 -0.08583721287460477 1107 84-85 -0.061765926605506394 1107 86-87 -0.04186938429391063 1107 88-89 -0.10842128132084383 1107 90-91 -0.0800471160844296 1107 92-93 -0.06645643339476948 1107 94-95 -0.08929690312411154 1107 96-97 -0.0898529554729457 1107 98-99 -0.10044116848158424 1107 100 -0.11986452645416179 1108 1 -0.01756416178863418 1108 2 -0.04605930914981826 1108 3 0.008928171409387176 1108 4 0.0623145760869761 1108 5 0.05261577886206936 1108 6 0.009453666118368176 1108 7 -0.016916807049604188 1108 8 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68375.0 15 96892.0 16 134603.0 17 174974.0 18 186867.0 19 195998.0 20 218276.0 21 267852.0 22 379633.0 23 690188.0 24 786414.0 25 608862.0 26 568305.0 27 535455.0 28 498359.0 29 540745.0 30 634572.0 31 772820.0 32 967654.0 33 1263665.0 34 1750954.0 35 2744534.0 36 4646159.0 37 7034878.0 38 6146914.0 39 1076458.0 40 1950.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.657279090243673 37.52917714600656 24.15926917017939 20.654274593570378 2 22.85564070991969 34.45056635386835 24.436100069684326 18.257692866527627 3 28.8942594961988 25.17673927848911 25.175177623915186 20.753823601396906 4 21.391405809438773 25.943071759266843 24.17475028351535 28.49077214777903 5 22.405523359611777 33.367611527034896 23.822117824074386 20.404747289278937 6 29.552490365997784 25.63599183997132 25.46702549905186 19.344492294979037 7 21.170655368238535 25.797985671296086 32.70090694444499 20.330452016020388 8 29.60632383292534 26.50606793481644 24.3822260964349 19.505382135823325 9 29.494345995072578 24.42068990771891 24.815537333858227 21.269426763350285 10-11 20.511791496330922 31.191734817751136 28.418718855922258 19.877754829995677 12-13 20.77317472043846 25.450541262895644 28.808883368467384 24.967400648198513 14-15 20.62018635944669 34.234822790247115 24.099057713926296 21.045933136379897 16-17 25.42449281979911 25.99222375877266 24.14708843176438 24.43619498966385 18-19 21.3421559491553 29.734534131997357 24.33086343508506 24.592446483762288 20-21 24.54956634576474 31.03484881288966 24.369836966652194 20.0457478746934 22-23 25.771681636087685 25.38129875040459 28.617844551023147 20.22917506248458 24-25 20.52858536338947 31.05151475424033 24.514638775495477 23.90526110687473 26-27 21.13595464322775 26.0367131001519 32.41063423101143 20.416698025608923 28-29 21.545742621977706 26.15334241508632 32.50867829073922 19.79223667219675 30-31 21.097208354041648 26.163356192905564 32.83656533348727 19.902870119565517 32-33 20.907272123279384 25.421158600645555 32.75589979221869 20.915669483856366 34-35 20.51209266654338 26.849824820934266 32.510375706354225 20.127706806168128 36-37 21.283795226261624 25.890923971015145 31.96658306469683 20.8586977380264 38-39 20.333710406814653 26.3647638324567 32.441882412758126 20.85964334797052 40-41 21.050912868560157 25.9131662346878 31.98590405859678 21.050016838155262 42-43 21.231497224262306 26.162487954304726 32.50477220147429 20.10124261995868 44-45 21.579361334057147 26.513332960632663 31.745318308595767 20.161987396714427 46-47 20.51450249511192 26.167414290797304 32.108196326532635 21.209886887558145 48-49 21.75296606866898 26.091227585985045 32.038407501115564 20.117398844230408 50-51 21.966920765902664 25.978347497594857 31.3937805000669 20.660951236435583 52-53 21.210674497169858 26.296992605810278 32.27398906461968 20.21834383240019 54-55 22.1945620236711 25.78773405296816 30.721645728133534 21.29605819522721 56-57 21.459570716829454 27.708597009673547 29.35938802817168 21.47244424532532 58-59 21.883028495998257 26.600254883877472 29.466854384690166 22.049862235434105 60-61 21.944020056452224 28.01514788488192 28.46970561345635 21.571126445209504 62-63 22.66980239034753 27.795419936487026 28.551362237912304 20.983415435253143 64-65 22.52597354228072 27.67248302937631 28.207628085919 21.59391534242397 66-67 22.73133036787188 28.346764685142485 28.00439520404633 20.91750974293931 68-69 24.240355657105063 26.71947808898192 28.324483744771683 20.715682509141335 70-71 23.65805918797817 28.327117060347252 27.900352833369812 20.114470918304765 72-73 24.19125064404061 27.15946087765576 28.50867973433509 20.140608743968535 74-75 24.368015217540577 27.73264610042706 28.257853519146447 19.64148516288591 76-77 24.098012473205685 28.27427812339388 27.627034166892162 20.000675236508275 78-79 24.240049600453734 27.547003066656732 26.92609696245324 21.286850370436287 80-81 24.23191803229154 28.226520925851347 26.753120789382816 20.7884402524743 82-83 25.100151091414713 27.429868679783702 26.591849963185503 20.87813026561608 84-85 24.560159524186275 27.851516928037963 27.2333240259719 20.354999521803865 86-87 25.045359662005616 27.54570833965072 27.048407881801424 20.360524116542244 88-89 25.293919072642744 27.638559808457014 27.045662600360693 20.021858518539553 90-91 25.67247817171837 27.778016956221734 26.513918627103973 20.03558624495592 92-93 25.646465975976547 27.457274214254955 26.497889573560794 20.3983702362077 94-95 26.40855632703292 27.486237175620904 26.81231534840024 19.29289114894594 96-97 26.374597377006587 27.631953785447212 26.57322955581039 19.420219281735807 98-99 25.883449089682927 28.15505172810118 26.70830468745079 19.253194494765104 100 26.221769003863233 28.573136408853873 25.904420938628046 19.300673648654847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 106411.0 1 62128.5 2 14236.0 3 10027.0 4 9386.0 5 9341.5 6 9613.5 7 9849.0 8 9672.5 9 9746.5 10 10798.5 11 16969.0 12 138803.5 13 161687.0 14 63156.5 15 60990.0 16 66946.0 17 73481.0 18 80951.0 19 89528.0 20 100772.5 21 118092.5 22 140906.0 23 167110.0 24 195727.5 25 227590.0 26 261165.0 27 296472.5 28 334940.5 29 368762.0 30 393994.0 31 417964.0 32 442370.0 33 462606.5 34 474783.0 35 479715.5 36 485481.5 37 490468.5 38 498845.0 39 506944.0 40 508654.5 41 512851.5 42 524582.0 43 535337.0 44 556278.5 45 640097.5 46 999942.0 47 2709108.0 48 4686978.5 49 4790035.0 50 3264234.5 51 1650592.5 52 888669.0 53 568895.0 54 418955.0 55 354411.5 56 317362.0 57 284200.0 58 249684.5 59 211788.5 60 173012.5 61 140284.0 62 111168.5 63 83551.5 64 61761.0 65 45536.0 66 33547.0 67 25694.0 68 19703.5 69 14890.5 70 11465.5 71 9233.0 72 7650.5 73 6177.0 74 4983.0 75 4117.5 76 3199.0 77 2153.5 78 1508.0 79 1158.0 80 840.5 81 631.0 82 509.5 83 420.0 84 348.0 85 277.5 86 220.0 87 182.5 88 146.5 89 124.5 90 112.5 91 90.5 92 70.0 93 56.0 94 47.0 95 43.0 96 35.0 97 22.5 98 16.5 99 11.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004250983348050435 2 9.319694751737162E-5 3 0.08691366944281333 4 0.024288327064285332 5 0.016826558556604157 6 0.05531990423442436 7 0.036115320339554356 8 0.024802413452203735 9 0.005080736816269613 10-11 0.007079961658174521 12-13 0.013714983050782236 14-15 0.025555504915207014 16-17 0.04804452962177793 18-19 0.061012433915848326 20-21 0.04380707486288324 22-23 0.02551942867745835 24-25 0.01807419511207865 26-27 0.016531935948323435 28-29 0.011708242326013023 30-31 0.009926978087172939 32-33 0.0073385080287065845 34-35 0.005360327658821729 36-37 0.009348255106621517 38-39 0.01372249893364654 40-41 0.015308350218014717 42-43 0.009274599454551335 44-45 0.0035129236507757656 46-47 0.0025764446458834667 48-49 0.0019225628366890046 50-51 0.0010898030153241036 52-53 0.0014776225711221982 54-55 2.87106725416419E-4 56-57 0.002519323936114755 58-59 0.010319307172689616 60-61 0.02152548852336712 62-63 0.0267535366437771 64-65 0.008551571523005275 66-67 0.0021871219135125115 68-69 0.0015542845763381007 70-71 0.0022006505026682583 72-73 7.155120486817563E-4 74-75 6.734231046416529E-4 76-77 0.0014205018613534866 78-79 0.0033054852837209707 80-81 0.007446736741952563 82-83 0.00870339235686422 84-85 0.00745876215453545 86-87 0.004542599603185436 88-89 0.002412598399441636 90-91 0.00265460982767223 92-93 0.0026876797122751686 94-95 0.003296466224283805 96-97 0.0034918791787557136 98-99 0.007772926058263365 100 0.013399315970481461 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.3262892E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.99293719997942 #Duplication Level Percentage of deduplicated Percentage of total 1 78.61597487743384 18.862281481494296 2 10.6587588729304 5.11469864535885 3 3.7695073635826923 2.7132466034789857 4 1.9337163781699522 1.855821424960133 5 1.1177338018947718 1.340885845757775 6 0.730060083468242 1.0509771440919158 7 0.4950440710985189 0.8314292916362237 8 0.3513288098102259 0.6743528056256212 9 0.27120267222645966 0.5856253814876444 >10 1.714270933064058 7.913750461306608 >50 0.2240729865585086 3.6902184713918116 >100 0.08641923874280324 4.455434773078427 >500 0.016825520830064455 2.8404266899873 >1k 0.011263565021661554 5.585797354934578 >5k 0.002358067017869759 3.9739228829682074 >10k+ 0.0014627581501224169 38.51113074244163 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2048008 6.157035293263135 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 413850 1.244179249356911 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 319770 0.9613415454074168 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 316488 0.9514746943831583 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 310424 0.9332441689075022 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 272753 0.8199918395550213 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 244022 0.733616307325292 No Hit AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA 242382 0.7286858881663085 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 239422 0.7197870828549725 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 234181 0.7040307860182452 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 225034 0.6765316737943291 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 223736 0.6726294274111824 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 220971 0.664316860963262 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 214347 0.6444027777260017 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 212760 0.6396316952837414 No Hit TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG 211590 0.6361142621032471 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 211427 0.6356242265404944 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 210387 0.632497619268944 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 209239 0.6290463258576555 No Hit TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG 208286 0.6261812713097826 No Hit TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT 202695 0.6093727508720529 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 200025 0.601345787972976 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 199863 0.6008587587633691 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 196683 0.5912985557599743 No Hit TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG 196520 0.5908085201972215 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 195939 0.5890618290195573 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 195655 0.5882080247261724 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 195247 0.5869814326427178 No Hit CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA 192883 0.579874413806232 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 189550 0.5698542387715415 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 186989 0.5621549683653484 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 186495 0.5606698299113618 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 182920 0.5499221174154069 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 179500 0.5396403896570389 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 177462 0.5335134419460581 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 176699 0.5312195944958724 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 172066 0.5172911603717439 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 172005 0.517107772829855 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 171860 0.5166718516237253 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 169294 0.5089575494518036 No Hit TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT 164556 0.4947134482473743 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 163945 0.49287656647533834 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 161454 0.48538774078934566 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 155962 0.4688768493130423 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 153479 0.46141207445221544 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 139933 0.42068801474026973 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 133960 0.4027310674008742 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115662 0.3477208175404592 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 106915 0.3214242465748318 Illumina Single End PCR Primer 1 (100% over 50bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 53554 0.16100223636597805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.3717265474090467 0.0 0.0 0.0 3.0063531457216648E-6 2 0.3717355664684839 0.0 0.0 0.0 3.0063531457216648E-6 3 0.3717355664684839 0.0 0.0 0.0 3.0063531457216648E-6 4 0.371744585527921 3.0063531457216648E-6 0.0 0.0 6.0127062914433295E-6 5 0.371744585527921 6.0127062914433295E-6 0.0 0.0 9.019059437164994E-6 6 0.371744585527921 9.019059437164994E-6 0.0 0.0 9.019059437164994E-6 7 0.371744585527921 1.2025412582886659E-5 0.0 0.0 1.2025412582886659E-5 8 0.37174759188106676 1.803811887432999E-5 0.0 0.0 1.2025412582886659E-5 9 0.37174759188106676 2.1044472020051653E-5 0.0 0.0 1.5031765728608324E-5 10-11 0.37177013952965965 2.5554001738634152E-5 0.0 0.0 1.6534942301469155E-5 12-13 0.3718062157674083 2.8560354884355814E-5 0.0 0.0 1.803811887432999E-5 14-15 0.37188738730234283 3.607623774865998E-5 0.0 0.0 2.4050825165773318E-5 16-17 0.3720331954299103 4.208894404010331E-5 0.0 0.0 2.4050825165773318E-5 18-19 0.37226468462213086 4.509529718582497E-5 0.0 0.0 2.4050825165773318E-5 20-21 0.37255028817097446 5.4114356622989966E-5 0.0 0.0 2.7057178311494983E-5 22-23 0.372965164905084 5.56175331958508E-5 0.0 0.0 2.7057178311494983E-5 24-25 0.373775377077856 6.0127062914433295E-5 0.0 0.0 2.7057178311494983E-5 26-27 0.37518685987977235 6.613976920587662E-5 0.0 1.5031765728608324E-6 2.7057178311494983E-5 28-29 0.3781150478437052 6.914612235159829E-5 0.0 4.509529718582497E-6 2.7057178311494983E-5 30-31 0.3850852776120609 7.064929892445913E-5 0.0 6.0127062914433295E-6 3.0063531457216648E-5 32-33 0.40267244351453263 8.718424122592828E-5 0.0 6.0127062914433295E-6 3.3069884602938316E-5 34-35 0.4512175309350732 9.31969475173716E-5 0.0 6.0127062914433295E-6 3.3069884602938316E-5 36-37 0.5569179011855012 9.31969475173716E-5 0.0 6.0127062914433295E-6 3.607623774865998E-5 38-39 0.7252751805224873 9.620330066309327E-5 0.0 6.0127062914433295E-6 4.058576746724247E-5 40-41 0.9458347758817844 1.0221600695453661E-4 0.0 6.0127062914433295E-6 4.659847375868581E-5 42-43 1.2064525237312498 1.0522236010025826E-4 0.0 6.0127062914433295E-6 5.1108003477268304E-5 44-45 1.5118093159187722 1.112350663917016E-4 0.0 6.0127062914433295E-6 5.261118005012913E-5 46-47 1.8564726723100324 1.1273824296456243E-4 0.0 6.0127062914433295E-6 5.56175331958508E-5 48-49 2.229911337835568 1.1724777268314494E-4 0.0 6.0127062914433295E-6 5.7120709768711634E-5 50-51 2.6379636503043695 1.2626683212030993E-4 0.0 6.0127062914433295E-6 5.7120709768711634E-5 52-53 3.0824740073713377 1.2777000869317075E-4 0.0 6.0127062914433295E-6 5.862388634157247E-5 54-55 3.5698414317071405 1.3528589155747492E-4 0.0 6.0127062914433295E-6 6.0127062914433295E-5 56-57 4.098681497688174 1.4129859784891825E-4 0.0 6.0127062914433295E-6 6.0127062914433295E-5 58-59 4.653897802993198 1.4280177442177908E-4 0.0 9.019059437164994E-6 6.313341606015496E-5 60-61 5.2310048687287924 1.4580812756750076E-4 0.0 9.019059437164994E-6 6.313341606015496E-5 62-63 5.831145109090334 1.533240104318049E-4 0.0 9.019059437164994E-6 6.613976920587663E-5 64-65 6.464071133682543 1.533240104318049E-4 0.0 9.019059437164994E-6 6.613976920587663E-5 66-67 7.1227796428524615 1.533240104318049E-4 0.0 9.019059437164994E-6 6.764294577873746E-5 68-69 7.807127834825667 1.533240104318049E-4 0.0 1.2025412582886659E-5 6.914612235159829E-5 70-71 8.506987305854224 1.578335401503874E-4 0.0 1.3528589155747491E-5 7.064929892445913E-5 72-73 9.224709024098086 1.5933671672324824E-4 0.0 1.5031765728608324E-5 7.215247549731995E-5 74-75 9.967060290488272 1.6835577616041325E-4 0.0 1.5031765728608324E-5 7.515882864304162E-5 76-77 10.727975787553289 1.7286530587899574E-4 0.0 1.5031765728608324E-5 7.515882864304162E-5 78-79 11.510732440221975 1.8489071846188238E-4 0.0 1.5031765728608324E-5 7.966835836162412E-5 80-81 12.305386735464854 2.0743836705479487E-4 0.0 1.5031765728608324E-5 8.117153493448495E-5 82-83 13.110812794028853 2.269796625019857E-4 0.0 1.5031765728608324E-5 8.117153493448495E-5 84-85 13.923736697338283 2.450177813763157E-4 0.0 1.5031765728608324E-5 8.267471150734577E-5 86-87 14.754952756362856 2.5854637053206316E-4 0.0 1.5031765728608324E-5 8.417788808020661E-5 88 15.384946083461413 2.7057178311494984E-4 0.0 1.5031765728608324E-5 8.417788808020661E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTT 302300 0.0 39.440033 20-21 TACTTTT 314965 0.0 37.94926 22-23 GAGTACT 297675 0.0 36.13471 20-21 GTACTTT 304780 0.0 35.24657 22-23 ACTTTTT 340490 0.0 31.995659 24-25 ACGCGAG 2390 0.0 31.18294 14-15 AGAGTAC 403825 0.0 30.226044 18-19 CGCGAGT 2265 0.0 28.95954 16-17 GCGAGTA 2715 0.0 27.62336 16-17 GTATCAC 12095 0.0 26.503633 9 AAGCGTG 5855 0.0 24.352663 1 GTGTATC 17735 0.0 24.293373 6 CTTTTTT 513335 0.0 23.712585 24-25 GTATAAC 6720 0.0 23.011957 9 GTTCAAC 8575 0.0 22.747879 9 ATCACGC 7860 0.0 22.279314 10-11 AACGCGA 3080 0.0 21.60187 14-15 ATAACGC 4240 0.0 21.287931 10-11 CGAGTAC 3555 0.0 21.227406 18-19 GTGGTTC 9750 0.0 20.695513 6 >>END_MODULE