FastQCFastQC Report
Thu 26 May 2016
SRR1660693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33262892
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16910925.084019753904742No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3544141.065493643787798No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG3462041.0408114844614231No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG3374561.01451190714265No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT3256140.9789106731910142No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA2976360.8947989248800134No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA2725430.8193605053944197No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA2596090.7804763338076557No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG2475360.744180632279358No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA2385480.7171595302056117No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC2317180.6966261382203328No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2296290.6903458664989202No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA2291400.6888757598106623No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT2242380.6741386166903346No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2226080.6692382610628084No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT2139930.6433385287124161No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2137370.6425689023071115No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC2122750.6381736140080664No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2115540.6360060333900011No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG2062180.6199641330044303No Hit
TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG2061790.6198468852317471No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2047620.6155868828242596No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2036850.6123490404863173No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT2033850.6114471345426008No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA2026700.6092975920434098No Hit
CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA2000540.601432972214202No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT1976040.5940674070071839No Hit
TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG1925430.5788522537366866No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1922730.5780405383873416No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA1909170.5739639235217431No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1908930.5738917710462458No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1875590.5638685896584097No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC1873410.5632132046726425No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1792980.5390331063216031No Hit
ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA1760300.5292083442413846No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1757800.5284567559549542No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC1752750.5269385476163648No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1748180.52556464422877No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1738170.5225552847299026No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC1709510.5139390766142643No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1683900.5062398062080711No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG1682130.5057076817012784No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT1554260.4672654440269355No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1534630.4613639728018839No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1464680.4403345325475608No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1409350.4237003805922828No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1229110.36951387149379555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA946370.2845122426516612No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG841070.25285534402721205TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT549100.16507885123157662No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376220.11310501804834047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTT2654700.037.57821320-21
TACTTTT2772150.036.03233322-23
GAGTACT2605850.034.4044520-21
GTACTTT2672700.033.47830622-23
ACTTTTT2955750.030.37840824-25
ACGCGAG20700.027.93400614-15
AGAGTAC3778700.027.06477718-19
CGCGAGT19550.025.64102716-17
GCGAGTA23250.024.8698116-17
GCTTGAA227400.024.16961960-61
CTTTTTT4284450.023.5005624-25
GTATCAC106100.023.2930349
GTATAAC61800.022.0323079
GTTCAAC81500.021.3983159
TGCTTGA265400.021.04499258-59
CTTGAAA268950.020.96702660-61
GTGGTTC90450.020.1573666
ATAACGC38750.019.31457910-11
GTGTATC171800.019.2979876
AACGCGA26750.018.82703214-15
Produced by FastQC (version 0.11.5)