##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660693_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33262892 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13900432950929 34.0 31.0 34.0 30.0 34.0 2 32.51761476422435 34.0 31.0 34.0 31.0 34.0 3 32.65221235122911 34.0 31.0 34.0 31.0 34.0 4 36.11401985131058 37.0 35.0 37.0 35.0 37.0 5 36.05073275047762 37.0 35.0 37.0 35.0 37.0 6 36.03373732506482 37.0 35.0 37.0 35.0 37.0 7 35.97238406089284 37.0 35.0 37.0 35.0 37.0 8 36.02116391443053 37.0 35.0 37.0 35.0 37.0 9 37.763940098774334 39.0 38.0 39.0 35.0 39.0 10-11 37.68391541240611 39.0 37.0 39.0 35.0 39.0 12-13 37.706798464787724 39.0 37.0 39.0 35.0 39.0 14-15 39.120531942923066 40.0 38.5 41.0 36.0 41.0 16-17 39.04724509522504 40.0 38.0 41.0 36.0 41.0 18-19 38.99627084439922 40.0 38.5 41.0 35.5 41.0 20-21 38.93843720804553 40.0 38.5 41.0 35.0 41.0 22-23 38.78284556556297 40.0 38.0 41.0 34.5 41.0 24-25 38.75527999790277 40.0 38.0 41.0 35.0 41.0 26-27 38.63055168203655 40.0 38.0 41.0 34.5 41.0 28-29 38.465358333845415 40.0 38.0 41.0 34.0 41.0 30-31 38.28855169899238 40.0 38.0 41.0 34.0 41.0 32-33 38.10907246128809 40.0 38.0 41.0 33.5 41.0 34-35 37.975997667310466 40.0 38.0 41.0 33.0 41.0 36-37 37.748114114671694 40.0 37.0 41.0 33.0 41.0 38-39 37.26219346772373 39.5 35.0 41.0 32.5 41.0 40-41 37.10824179088216 39.0 35.0 41.0 32.0 41.0 42-43 37.07933717849909 39.0 35.0 41.0 32.0 41.0 44-45 37.18862309086053 40.0 35.0 41.0 32.5 41.0 46-47 37.023729310728605 39.5 35.0 41.0 31.5 41.0 48-49 36.899266756480465 39.0 35.0 41.0 31.0 41.0 50-51 36.70983954131228 39.0 35.0 41.0 31.0 41.0 52-53 36.18237228139995 39.0 35.0 41.0 28.5 41.0 54-55 35.76957639462017 39.0 35.0 41.0 26.5 41.0 56-57 35.34740408320479 39.0 35.0 41.0 23.5 41.0 58-59 35.011626213980435 39.0 35.0 40.5 22.0 41.0 60-61 34.745197831866214 38.5 34.5 40.0 22.0 41.0 62-63 34.385014087169566 37.5 34.0 40.0 21.0 41.0 64-65 33.936633892206366 37.0 34.0 39.5 20.0 41.0 66-67 33.46215533514043 36.5 33.0 39.0 19.0 41.0 68-69 32.99886120846017 36.0 32.5 39.0 19.0 40.0 70-71 32.462421592806784 35.5 32.0 37.5 18.0 39.0 72-73 30.767898203198925 35.0 30.0 37.0 9.5 39.0 74-75 29.919676572319688 35.0 28.5 36.0 9.0 37.5 76-77 29.496673695720744 33.0 27.5 34.5 11.0 36.0 78-79 30.83135777851186 34.5 31.0 35.0 12.0 37.0 80-81 30.99718884936403 35.0 31.0 35.0 12.0 36.5 82-83 30.82845122125881 35.0 31.0 35.0 11.0 36.0 84-85 30.67017111741216 35.0 31.0 35.0 11.0 36.0 86-87 30.479061967913072 35.0 31.0 35.0 10.5 35.5 88-89 30.32008168441878 35.0 31.0 35.0 10.0 35.0 90-91 30.234778398102005 34.0 31.0 35.0 10.0 35.0 92-93 30.11521370420828 34.0 31.0 35.0 10.0 35.0 94-95 30.024339044241856 34.0 30.5 35.0 10.0 35.0 96-97 29.90985579365739 34.0 30.0 35.0 10.0 35.0 98-99 29.765794101727536 34.0 30.0 35.0 10.0 35.0 100 29.71710051549336 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5552052673983141 1101 2 0.39235877762254745 1101 3 0.32697516327976217 1101 4 0.2400192631164586 1101 5 0.2263642639696215 1101 6 0.22527576313483877 1101 7 0.2526071842068873 1101 8 0.27677902763822715 1101 9 0.3514086292022114 1101 10-11 0.33856141451864374 1101 12-13 0.33818488926030454 1101 14-15 0.3776667202162898 1101 16-17 0.40831230552463893 1101 18-19 0.42832950926872826 1101 20-21 0.38811442536052 1101 22-23 0.3804497834984275 1101 24-25 0.3940230002157037 1101 26-27 0.39510376324252405 1101 28-29 0.4062910941871465 1101 30-31 0.41931862096304684 1101 32-33 0.48521905226682804 1101 34-35 0.4207811718108374 1101 36-37 0.42427767076692646 1101 38-39 0.4503920634248644 1101 40-41 0.4645876656538377 1101 42-43 0.42414709006687445 1101 44-45 0.4453917544448984 1101 46-47 0.4281373078684183 1101 48-49 0.36953997395320215 1101 50-51 0.3290017021490357 1101 52-53 0.28641187822870506 1101 54-55 0.2831138537217939 1101 56-57 0.23996663400314588 1101 58-59 0.3233810643290198 1101 60-61 0.24328262094801545 1101 62-63 0.27216111024556966 1101 64-65 0.27822737658092933 1101 66-67 0.24932467002054182 1101 68-69 0.28943417114701475 1101 70-71 0.28733332412898704 1101 72-73 1.4582944017913384 1101 74-75 1.9208354188898795 1101 76-77 0.7033893033393888 1101 78-79 0.4762410810948161 1101 80-81 0.3530560006932255 1101 82-83 0.3544889321959239 1101 84-85 0.26555517652149163 1101 86-87 0.2968769199199208 1101 88-89 0.29856932265061786 1101 90-91 0.21896039043814497 1101 92-93 0.13740948277000697 1101 94-95 0.16604812782079037 1101 96-97 0.18662752467119503 1101 98-99 0.1734485086896278 1101 100 0.16434305927775483 1102 1 0.5034829769796403 1102 2 0.3541261567532956 1102 3 0.2841156564112879 1102 4 0.21149899003111017 1102 5 0.18274586175951413 1102 6 0.20253300946424702 1102 7 0.22483347283619537 1102 8 0.20828583384783173 1102 9 0.2676141466155997 1102 10-11 0.27251130636247467 1102 12-13 0.26277226027982437 1102 14-15 0.3209690184952336 1102 16-17 0.32870516956841556 1102 18-19 0.3438704403011954 1102 20-21 0.32011766406944986 1102 22-23 0.2901767104519024 1102 24-25 0.33678184581776094 1102 26-27 0.33035085699257394 1102 28-29 0.3521375738285428 1102 30-31 0.33526273575428434 1102 32-33 0.3419890324751549 1102 34-35 0.3557923465066466 1102 36-37 0.32399942601955445 1102 38-39 0.38092470575860915 1102 40-41 0.41295321481519665 1102 42-43 0.377847804781716 1102 44-45 0.3882030732264141 1102 46-47 0.3444217724736305 1102 48-49 0.29821670922961374 1102 50-51 0.27098740766926 1102 52-53 0.14719195441038124 1102 54-55 0.128650950746227 1102 56-57 0.13114165011126744 1102 58-59 0.21875562731793963 1102 60-61 0.21383314053259284 1102 62-63 0.22506867654200136 1102 64-65 0.12293404211580139 1102 66-67 0.1715875901518018 1102 68-69 0.1779817214927064 1102 70-71 0.22691489182320623 1102 72-73 1.4469746281004383 1102 74-75 1.8682728295860898 1102 76-77 0.5883478515961862 1102 78-79 0.3489835089808082 1102 80-81 0.264615945804227 1102 82-83 0.23856115553940072 1102 84-85 0.21339819027909357 1102 86-87 0.23815734130113242 1102 88-89 0.2133887043959639 1102 90-91 0.152106718759633 1102 92-93 0.14559619782285793 1102 94-95 0.16578912165434545 1102 96-97 0.13676040341636764 1102 98-99 0.12258306511187556 1102 100 0.15605858785058402 1103 1 0.4650135672240907 1103 2 0.33280327733804427 1103 3 0.24135275153259528 1103 4 0.18370002116606088 1103 5 0.19290300582611764 1103 6 0.1742646084983619 1103 7 0.17790767855184697 1103 8 0.19131457191087975 1103 9 0.21351004284989017 1103 10-11 0.22725679171115587 1103 12-13 0.24701767381243656 1103 14-15 0.25485623797253254 1103 16-17 0.30649214698085103 1103 18-19 0.32736570318580505 1103 20-21 0.28261664836561096 1103 22-23 0.2733904974796886 1103 24-25 0.2916711927068292 1103 26-27 0.25487084413165206 1103 28-29 0.32838570787763643 1103 30-31 0.2743454845664246 1103 32-33 0.3358177590033975 1103 34-35 0.32973422058098123 1103 36-37 0.317183911423939 1103 38-39 0.3446401454166903 1103 40-41 0.3738581499642777 1103 42-43 0.3099746916302166 1103 44-45 0.2919246864217655 1103 46-47 0.2742854603000566 1103 48-49 0.21280868377449025 1103 50-51 0.2298241115430173 1103 52-53 0.1008940168785557 1103 54-55 0.11242868258545258 1103 56-57 0.13191856968128235 1103 58-59 0.21876775530697756 1103 60-61 0.16493909959429942 1103 62-63 0.15516087036345994 1103 64-65 0.1365810050094325 1103 66-67 0.1596115996697165 1103 68-69 0.15350599875501558 1103 70-71 0.14058986019080777 1103 72-73 1.3340615239402887 1103 74-75 1.7948250246846804 1103 76-77 0.45424619001476074 1103 78-79 0.30069455788290256 1103 80-81 0.2418814887984304 1103 82-83 0.18771359134793641 1103 84-85 0.1385916249093384 1103 86-87 0.20487273542423168 1103 88-89 0.1514223882544492 1103 90-91 0.1403962384902364 1103 92-93 0.12650803759180107 1103 94-95 0.10401248719166745 1103 96-97 0.1277003925597313 1103 98-99 0.07480662971710217 1103 100 0.09728532736158257 1104 1 0.41644316930727143 1104 2 0.2933796257536727 1104 3 0.19272021182845123 1104 4 0.13938542492475392 1104 5 0.1299373599191398 1104 6 0.12391910203579215 1104 7 0.1760605445275445 1104 8 0.175277803812115 1104 9 0.19745170547813018 1104 10-11 0.17993157047235542 1104 12-13 0.21142879577156037 1104 14-15 0.21704108764279795 1104 16-17 0.24845017462539687 1104 18-19 0.26450239005495035 1104 20-21 0.1991924881575713 1104 22-23 0.19224755959169926 1104 24-25 0.2360714895005529 1104 26-27 0.22037305278508512 1104 28-29 0.2137277235342836 1104 30-31 0.20919085231503942 1104 32-33 0.27614018454524825 1104 34-35 0.2776955158324981 1104 36-37 0.2624717199776896 1104 38-39 0.2903110497447656 1104 40-41 0.2786494577044465 1104 42-43 0.24822413638849383 1104 44-45 0.25927123960288867 1104 46-47 0.22417222984687868 1104 48-49 0.2458026012460479 1104 50-51 0.20740403281219955 1104 52-53 0.15266648112540793 1104 54-55 0.11455712028412535 1104 56-57 0.15864692783341638 1104 58-59 0.20765829070256814 1104 60-61 0.16844970616084964 1104 62-63 0.16580123898008026 1104 64-65 0.17244107847842827 1104 66-67 0.16552329134019317 1104 68-69 0.23180953376035518 1104 70-71 0.1961228362180023 1104 72-73 1.4302145518266975 1104 74-75 1.8719234540927836 1104 76-77 0.5596231768241893 1104 78-79 0.3540474976094501 1104 80-81 0.25051304322925816 1104 82-83 0.25345850789966917 1104 84-85 0.18833175773708177 1104 86-87 0.2490272043129238 1104 88-89 0.23698151079266339 1104 90-91 0.17665537457674318 1104 92-93 0.13791563659656347 1104 94-95 0.11837164177714854 1104 96-97 0.12047012360569198 1104 98-99 0.152484669431459 1104 100 0.12219235569572717 1105 1 0.3666313313972154 1105 2 0.2480742948584549 1105 3 0.17183256850439932 1105 4 0.1214005682600785 1105 5 0.10781417827806905 1105 6 0.12933279135339149 1105 7 0.11971667196404212 1105 8 0.1171158240669854 1105 9 0.11483455634380135 1105 10-11 0.15098617559930716 1105 12-13 0.14405047829483664 1105 14-15 0.15281060790659495 1105 16-17 0.1879754963815614 1105 18-19 0.2184162937797609 1105 20-21 0.15433717298973448 1105 22-23 0.13361374312712115 1105 24-25 0.18208296877642027 1105 26-27 0.1507766820948504 1105 28-29 0.200617697594474 1105 30-31 0.17064651265031472 1105 32-33 0.24547797461946175 1105 34-35 0.20291521197983542 1105 36-37 0.1465208898200956 1105 38-39 0.15492546682708763 1105 40-41 0.1543333548802437 1105 42-43 0.1630412011240523 1105 44-45 0.14895422430764427 1105 46-47 0.15708192510602714 1105 48-49 0.11423205332386033 1105 50-51 0.09351957095097418 1105 52-53 0.04984792750092737 1105 54-55 -0.055917538227618024 1105 56-57 -0.03195462763338952 1105 58-59 0.07888790366141762 1105 60-61 0.015871263763621357 1105 62-63 -0.026522651635367822 1105 64-65 0.030451884649131955 1105 66-67 5.84252462118684E-4 1105 68-69 0.0322156916327998 1105 70-71 0.04310926380249214 1105 72-73 1.2667353846604463 1105 74-75 1.7705520135833197 1105 76-77 0.4018368485737014 1105 78-79 0.24125409452438973 1105 80-81 0.11918942735885096 1105 82-83 0.06436085750932463 1105 84-85 0.031755648848271534 1105 86-87 0.09450281039356412 1105 88-89 0.07544539190017119 1105 90-91 0.028403554496350125 1105 92-93 -0.017676486151582793 1105 94-95 0.010422431152083078 1105 96-97 0.05398728065079439 1105 98-99 0.026411563197584087 1105 100 -0.011958869671385486 1106 1 0.34603654003244344 1106 2 0.2107010613076099 1106 3 0.12624119532021183 1106 4 0.08973218507109237 1106 5 0.07580358996136027 1106 6 0.09384519361456256 1106 7 0.07981665686007489 1106 8 0.08277321693737605 1106 9 0.09127815591912736 1106 10-11 0.10618455079361411 1106 12-13 0.10954779505221524 1106 14-15 0.06976717132896937 1106 16-17 0.08544212706484444 1106 18-19 0.130662540043069 1106 20-21 0.09260259035496432 1106 22-23 0.06346719629521402 1106 24-25 0.11848713284428669 1106 26-27 0.10331170583484806 1106 28-29 0.1301802412463573 1106 30-31 0.08092085739544785 1106 32-33 0.16387003718796933 1106 34-35 0.1732357042996 1106 36-37 0.1491638982903538 1106 38-39 0.15834439372743958 1106 40-41 0.18718225659475252 1106 42-43 0.17073844659518755 1106 44-45 0.18241921775219794 1106 46-47 0.1163670395035652 1106 48-49 0.09653730918648051 1106 50-51 0.03713105862048849 1106 52-53 0.0015557731723347956 1106 54-55 -0.07791113625557244 1106 56-57 -0.09159083287892855 1106 58-59 -0.008802078060277552 1106 60-61 -0.0264316043920374 1106 62-63 -0.010473893911942866 1106 64-65 0.017987307176468903 1106 66-67 0.03904894972397699 1106 68-69 0.06309334797706612 1106 70-71 0.10789694160835239 1106 72-73 1.3224954108758915 1106 74-75 1.7478946379866933 1106 76-77 0.35497845997120336 1106 78-79 0.2519359473281746 1106 80-81 0.12275738569502437 1106 82-83 0.0680776981131821 1106 84-85 0.034745528387098545 1106 86-87 0.11560093341132927 1106 88-89 0.11379568679659968 1106 90-91 0.034716360449845496 1106 92-93 0.03541076079982375 1106 94-95 0.07620171971239031 1106 96-97 0.09432484090348936 1106 98-99 0.1004167433377674 1106 100 0.08051226728527894 1107 1 0.3137073665313608 1107 2 0.1976536195936447 1107 3 0.10375131638989643 1107 4 0.08503408573853477 1107 5 0.0729360384375326 1107 6 0.06974604377032989 1107 7 0.0835186753565651 1107 8 0.08178943874075628 1107 9 0.09980768170112952 1107 10-11 0.10472953022996734 1107 12-13 0.1202133762393558 1107 14-15 0.09445884433947072 1107 16-17 0.09983396006790457 1107 18-19 0.11294626729867474 1107 20-21 0.07021466546712674 1107 22-23 0.08928113199996801 1107 24-25 0.1093814180072954 1107 26-27 0.04922147471625493 1107 28-29 0.03986134388904361 1107 30-31 0.08186381590770964 1107 32-33 0.10882964044123611 1107 34-35 0.10273624442739049 1107 36-37 0.10047898298063984 1107 38-39 0.10551394022946425 1107 40-41 0.10588430997104581 1107 42-43 0.04039717397289877 1107 44-45 0.10789412823116606 1107 46-47 0.08587103679045072 1107 48-49 0.06985665014789078 1107 50-51 0.013633938235706466 1107 52-53 -0.03507781198855042 1107 54-55 -0.012127659123216006 1107 56-57 -0.056117033829501395 1107 58-59 0.03281389995692763 1107 60-61 -0.08003161770736256 1107 62-63 -0.12762568721542067 1107 64-65 -0.0424682937219103 1107 66-67 -0.09956076891262455 1107 68-69 -0.07563417540360007 1107 70-71 -0.03281720166125268 1107 72-73 1.2243803638328608 1107 74-75 1.6610294915499892 1107 76-77 0.2878140083506473 1107 78-79 0.12101371989163923 1107 80-81 0.016983459435927273 1107 82-83 0.04248608409196564 1107 84-85 -0.029424261099393334 1107 86-87 0.03369465236306013 1107 88-89 0.042787616620113766 1107 90-91 -0.02154058452289931 1107 92-93 -0.05890557112878625 1107 94-95 -0.06384329967405833 1107 96-97 -0.04357056926031788 1107 98-99 -0.03667724095269165 1107 100 -0.04882449785336718 1108 1 0.28786181520857923 1108 2 0.15285518628663652 1108 3 0.08022331967880092 1108 4 0.04548570198181778 1108 5 0.0369431926788053 1108 6 0.04543846049572409 1108 7 0.06358906177712953 1108 8 0.028373178745454197 1108 9 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-1.2037397955930587 2316 66-67 -1.0768056649372681 2316 68-69 -1.0577151081633218 2316 70-71 -2.168687503237109 2316 72-73 -0.5723832690273696 2316 74-75 0.6121304468560034 2316 76-77 -0.4352131320121977 2316 78-79 -1.8234639976908902 2316 80-81 -1.7051009228193728 2316 82-83 -1.0018503226127322 2316 84-85 -0.8668365185106879 2316 86-87 -0.8528015452130049 2316 88-89 -1.036652960280751 2316 90-91 -1.1707601893351196 2316 92-93 -1.0610524144135773 2316 94-95 -1.080390726525156 2316 96-97 -1.1023984780113594 2316 98-99 -1.1720182871669635 2316 100 -1.164826703039946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 24.0 7 475.0 8 918.0 9 1564.0 10 6238.0 11 8469.0 12 6465.0 13 9585.0 14 22500.0 15 47242.0 16 81663.0 17 120459.0 18 142314.0 19 164015.0 20 201792.0 21 215799.0 22 286194.0 23 521265.0 24 687648.0 25 552967.0 26 503655.0 27 476133.0 28 486855.0 29 547788.0 30 637521.0 31 745331.0 32 916504.0 33 1185124.0 34 1667603.0 35 2702442.0 36 5025755.0 37 7689878.0 38 6573177.0 39 1025718.0 40 1812.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.972134156194237 35.924073139781804 24.41273438178733 21.691058322236632 2 23.686244641961302 32.97054544690385 25.456690133572657 17.886519777562196 3 27.372469597652543 25.924679068795342 25.412778299613876 21.29007303393824 4 21.76395677552255 26.800572190359212 24.661391253782767 26.77407978033547 5 23.43140931231109 31.893164857493307 24.05405131581214 20.62137451438346 6 27.821552617854156 26.28021339816153 25.97022832530617 19.928005658678146 7 21.706239493547343 26.638246006991817 31.124323164684537 20.531191334776302 8 28.17124560305821 26.928770955934922 25.060650168361786 19.839333272645085 9 28.29619565250069 24.92464275204934 25.1466168365637 21.63254475888627 10-11 21.123566164962444 30.890262037347803 27.794235389995553 20.191936407694197 12-13 21.308322016166425 25.817171202417207 28.487155760884146 24.387351020532222 14-15 21.053036329176436 33.20148715930452 24.245121716613887 21.500354794905157 16-17 24.945997811727416 26.849242082844547 24.484594192114052 23.720165913313988 18-19 21.785571140356648 29.365871133514187 24.765901293248945 24.08265643288022 20-21 24.07368848144653 30.71275943174153 24.677286027925653 20.536266058886284 22-23 25.370280491545955 26.059002927346185 28.156072538731745 20.41464404237611 24-25 20.90915822777046 30.676548711730927 24.841449750374792 23.572843310123815 26-27 21.284801083511926 26.43677704642166 31.57559464391525 20.702827226151165 28-29 21.670836674024173 26.58333179298966 31.469543059067785 20.276288473918385 30-31 21.31834091221439 26.91028009501315 31.648104419682078 20.123274573090384 32-33 21.110512589906 26.269824389184272 31.15166210548974 21.46800091541999 34-35 20.870253063850317 27.050649468422233 31.351606889342804 20.72749057838465 36-37 21.967163043441786 26.382453410515588 30.67703239662034 20.973351149422285 38-39 20.823746570868558 26.893255812287897 31.452952656635347 20.830044960208202 40-41 21.114943268660095 26.804758700414787 30.976463690829465 21.103834340095652 42-43 21.405230662488897 26.76569795979919 31.31956427101219 20.509507106699722 44-45 21.579711739888864 27.005780439312428 30.843484801213577 20.571023019585134 46-47 21.142174697514022 26.654694184929845 30.714714402616 21.488416714940133 48-49 21.893701247636724 26.870552151966276 31.17265475790319 20.06309184249381 50-51 22.451770766611627 26.600408446895035 30.19457526039743 20.753245526095913 52-53 21.397935192381766 27.036666268621225 30.738407324221427 20.826991214775585 54-55 22.10333260329876 26.58620701007771 29.690664688173296 21.619795698450233 56-57 21.57964556227142 28.198545600247808 28.4630331315855 21.758775705895268 58-59 21.905989643351454 27.039838919868608 28.621582732683027 22.432588704096915 60-61 22.05336339510751 28.051914139184152 27.58076398087589 22.313958484832447 62-63 22.254295940370632 28.19409659264386 28.10362156264057 21.44798590434494 64-65 22.218034850522557 28.036389504973442 27.459571587280067 22.28600405722393 66-67 21.75459506013886 29.20044436712183 27.158087362170242 21.886873210569068 68-69 23.09863944663881 27.684471072493267 27.35207789081835 21.864811590049577 70-71 22.23196466296671 29.155905469188916 26.98641603876632 21.625713829078055 72-73 22.909766461730204 28.18429752431851 27.255844539765718 21.65009147418557 74-75 22.367613740203875 28.517465884050196 27.335159886805936 21.779760488939985 76-77 21.708469641910906 29.173018713716232 26.940163577089482 22.17834806728338 78-79 21.62614466877151 28.49097098592254 26.149432757180573 23.733451588125373 80-81 21.37102173554683 29.02318421363821 26.205576676763542 23.40021737405142 82-83 22.145019385802815 28.322271251712213 25.9415703420471 23.591139020437872 84-85 21.192600310047197 28.7634207586811 26.63719849863772 23.406780432633983 86-87 21.27813283725175 28.587622173967002 26.386888045570604 23.747356943210637 88-89 21.371768050597836 28.601924971817294 26.3096522307876 23.716654746797268 90-91 21.508821592976464 28.60336225100995 25.856264504624704 24.03155165138888 92-93 21.31712054350123 28.157669112395624 25.674350836530362 24.850859507572785 94-95 21.88018392688458 28.07531841251116 26.202281527945253 23.842216132659008 96-97 21.861069508130328 27.978061913741815 25.979680799667133 24.181187778460725 98-99 20.945883039333918 28.383032161789945 26.31414469189213 24.356940106984005 100 21.13010768836374 28.538133610469078 25.869967234681596 24.461791466485586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 92572.0 1 57220.0 2 16644.5 3 10558.0 4 9639.0 5 9741.0 6 10099.5 7 10356.5 8 10522.5 9 10865.0 10 12325.0 11 19195.5 12 130707.5 13 153159.0 14 62508.0 15 57701.5 16 63046.0 17 68843.0 18 75228.0 19 82843.5 20 93618.0 21 109520.0 22 131326.5 23 159383.5 24 193802.0 25 230464.0 26 258111.5 27 285742.5 28 324039.0 29 362612.0 30 394113.0 31 423532.5 32 453927.5 33 479643.0 34 492306.0 35 493934.5 36 494231.5 37 490787.0 38 490457.0 39 491041.5 40 489250.0 41 491984.5 42 501343.0 43 513370.0 44 538263.0 45 631773.0 46 1044721.0 47 2828587.5 48 4794860.5 49 4760204.0 50 3215487.0 51 1707081.0 52 919015.5 53 541867.5 54 399204.5 55 348666.5 56 315341.5 57 282289.0 58 246934.5 59 208053.5 60 168502.5 61 135450.5 62 106179.5 63 78654.5 64 57619.0 65 42030.5 66 30569.5 67 23116.0 68 17355.5 69 12733.0 70 9706.5 71 7819.0 72 6388.5 73 4922.5 74 3793.5 75 3185.5 76 2360.0 77 1412.5 78 871.5 79 608.0 80 419.0 81 284.5 82 191.0 83 111.0 84 61.5 85 54.0 86 39.5 87 21.0 88 11.5 89 11.0 90 9.0 91 3.5 92 3.5 93 4.5 94 2.0 95 0.0 96 1.0 97 1.0 98 0.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20094464426003605 2 6.313341606015496E-5 3 0.0 4 0.0016083989329610908 5 8.387725276563445E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.019059437164994E-6 14-15 3.908259089438164E-5 16-17 0.0017512007073828696 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005495613550379203 26-27 0.030909819867737293 28-29 0.054554787358838196 30-31 0.08581484736805206 32-33 0.09036646603067466 34-35 0.09777712653487856 36-37 0.10484205642732448 38-39 0.09672640611044885 40-41 0.05820900960746288 42-43 0.04130278269249709 44-45 0.04531476096546265 46-47 0.08605385244313693 48-49 0.11374837762152491 50-51 0.1282555347262048 52-53 0.47942012979508813 54-55 0.4655969180310599 56-57 0.47137963830685564 58-59 0.46418543522914363 60-61 0.4821829683360064 62-63 0.470554394368355 64-65 0.45126262623225905 66-67 0.4569100606164972 68-69 0.4663244554923246 70-71 0.4733593218533133 72-73 1.1559142241750957 74-75 3.151573531249177 76-77 0.47228154425057206 78-79 0.48209127456506184 80-81 0.5060458964301722 82-83 0.5168612518719058 84-85 0.5155249278986325 86-87 0.4899153086268025 88-89 0.46810722290773754 90-91 0.4424179352775459 92-93 0.4302512240968104 94-95 0.4338077398681991 96-97 0.45280037586629573 98-99 0.4467350583948022 100 0.4476910786951417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.3262892E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.2010689575203 #Duplication Level Percentage of deduplicated Percentage of total 1 77.12670339904345 17.894219640274223 2 11.40762384428757 5.293381349055375 3 4.066278665242862 2.8302603513837967 4 1.9891024047015495 1.845972082202004 5 1.197769783273381 1.3894769668479925 6 0.8132980598705732 1.1321630622044785 7 0.5355820544630161 0.8698253324604779 8 0.38054557306969106 0.7063251265815186 9 0.28806983042164463 0.6015175200174381 >10 1.8263118657881476 8.1353053698821 >50 0.24046171839801714 3.8266617287686198 >100 0.09390809076324365 4.622125774813368 >500 0.017629835083231026 2.8398415084760447 >1k 0.012724043660259865 5.880410898742995 >5k 0.0024942699585530676 4.113462595077018 >10k+ 0.0014965619751318405 38.019050693212755 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1691092 5.084019753904742 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 354414 1.065493643787798 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 346204 1.0408114844614231 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 337456 1.01451190714265 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 325614 0.9789106731910142 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 297636 0.8947989248800134 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 272543 0.8193605053944197 No Hit AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA 259609 0.7804763338076557 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 247536 0.744180632279358 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 238548 0.7171595302056117 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 231718 0.6966261382203328 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 229629 0.6903458664989202 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 229140 0.6888757598106623 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 224238 0.6741386166903346 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 222608 0.6692382610628084 No Hit TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT 213993 0.6433385287124161 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 213737 0.6425689023071115 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 212275 0.6381736140080664 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 211554 0.6360060333900011 No Hit TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG 206218 0.6199641330044303 No Hit TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG 206179 0.6198468852317471 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 204762 0.6155868828242596 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 203685 0.6123490404863173 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 203385 0.6114471345426008 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 202670 0.6092975920434098 No Hit CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA 200054 0.601432972214202 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 197604 0.5940674070071839 No Hit TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG 192543 0.5788522537366866 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 192273 0.5780405383873416 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 190917 0.5739639235217431 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 190893 0.5738917710462458 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 187559 0.5638685896584097 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 187341 0.5632132046726425 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 179298 0.5390331063216031 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 176030 0.5292083442413846 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 175780 0.5284567559549542 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 175275 0.5269385476163648 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 174818 0.52556464422877 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 173817 0.5225552847299026 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 170951 0.5139390766142643 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 168390 0.5062398062080711 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 168213 0.5057076817012784 No Hit TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT 155426 0.4672654440269355 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 153463 0.4613639728018839 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 146468 0.4403345325475608 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 140935 0.4237003805922828 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 122911 0.36951387149379555 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94637 0.2845122426516612 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG 84107 0.25285534402721205 TruSeq Adapter, Index 7 (97% over 35bp) AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 54910 0.16507885123157662 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37622 0.11310501804834047 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.36983254492724205 0.0 0.0 0.0 3.0063531457216648E-5 2 0.3698415639866792 0.0 0.0 0.0 3.0063531457216648E-5 3 0.3698415639866792 0.0 0.0 0.0 3.3069884602938316E-5 4 0.36984757669297064 0.0 0.0 0.0 3.607623774865998E-5 5 0.36984757669297064 3.0063531457216648E-6 0.0 0.0 3.607623774865998E-5 6 0.36984757669297064 1.2025412582886659E-5 0.0 0.0 3.607623774865998E-5 7 0.3698505830461164 1.2025412582886659E-5 0.0 0.0 3.607623774865998E-5 8 0.3698535893992621 1.5031765728608324E-5 0.0 0.0 3.607623774865998E-5 9 0.3698535893992621 1.803811887432999E-5 0.0 0.0 3.607623774865998E-5 10-11 0.3698731306947093 2.1044472020051653E-5 0.0 0.0 3.607623774865998E-5 12-13 0.36991972916846794 2.4050825165773318E-5 0.0 0.0 3.607623774865998E-5 14-15 0.37002194517542253 2.7057178311494983E-5 0.0 0.0 4.359212061296414E-5 16-17 0.37016625012641713 2.8560354884355814E-5 0.0 0.0 4.8101650331546636E-5 18-19 0.37039773931863773 3.457306117579915E-5 0.0 0.0 4.8101650331546636E-5 20-21 0.3706818396909084 4.2088944040103313E-5 0.0 0.0 4.8101650331546636E-5 22-23 0.3711132513673195 4.8101650331546636E-5 0.0 0.0 4.8101650331546636E-5 24-25 0.3719099349509357 4.8101650331546636E-5 0.0 1.5031765728608324E-6 5.1108003477268304E-5 26-27 0.3732973669276863 4.8101650331546636E-5 0.0 4.509529718582497E-6 5.1108003477268304E-5 28-29 0.3762120263024634 6.163023948729414E-5 0.0 6.0127062914433295E-6 5.4114356622989966E-5 30-31 0.3830980781827389 6.313341606015496E-5 0.0 6.0127062914433295E-6 5.4114356622989966E-5 32-33 0.40053492642792454 6.914612235159829E-5 0.0 6.0127062914433295E-6 5.4114356622989966E-5 34-35 0.4485959909920039 7.966835836162412E-5 0.0 6.0127062914433295E-6 5.56175331958508E-5 36-37 0.5531344057516105 9.470012409023245E-5 0.0 6.0127062914433295E-6 5.7120709768711634E-5 38-39 0.719554090486179 9.920965380881494E-5 0.0 9.019059437164994E-6 6.0127062914433295E-5 40-41 0.9377071001523258 1.0672553667311909E-4 0.0 9.019059437164994E-6 6.0127062914433295E-5 42-43 1.1950599484855378 1.0973188981884077E-4 0.0 9.019059437164994E-6 6.313341606015496E-5 44-45 1.4967625184244353 1.1273824296456243E-4 0.0 9.019059437164994E-6 6.613976920587663E-5 46-47 1.8375461760811418 1.2025412582886659E-4 0.0 9.019059437164994E-6 6.613976920587663E-5 48-49 2.206111543157462 1.2025412582886659E-4 0.0 1.0522236010025827E-5 6.613976920587663E-5 50-51 2.6094153208325963 1.2326047897458827E-4 0.0 1.2025412582886659E-5 7.666200521590245E-5 52-53 3.0487788013140893 1.3077636183889244E-4 0.0 1.6534942301469155E-5 8.267471150734577E-5 54-55 3.529936603227404 1.397954212760574E-4 0.0 1.803811887432999E-5 8.417788808020661E-5 56-57 4.05276696927014 1.4280177442177908E-4 0.0 1.803811887432999E-5 8.417788808020661E-5 58-59 4.600547060069221 1.4731130414036156E-4 0.0 1.803811887432999E-5 8.568106465306745E-5 60-61 5.165693349814562 1.578335401503874E-4 0.0 1.803811887432999E-5 9.169377094451078E-5 62-63 5.729041840378762 1.623430698689699E-4 0.0 1.9541295447190823E-5 9.31969475173716E-5 64-65 6.292979576159523 1.6384624644183074E-4 0.0 2.2547648592912484E-5 9.31969475173716E-5 66-67 6.882421708852014 1.713621293061349E-4 0.0 2.4050825165773318E-5 9.920965380881494E-5 68-69 7.492739957788396 1.7286530587899574E-4 0.0 2.7057178311494983E-5 9.920965380881494E-5 70-71 8.120719028279321 1.7737483559757822E-4 0.0 3.0063531457216648E-5 9.920965380881494E-5 72-73 8.773725988708378 1.8188436531616073E-4 0.0 3.156670803007748E-5 1.0071283038167578E-4 74-75 9.481561314632534 1.894002481804649E-4 0.0 3.3069884602938316E-5 1.0221600695453661E-4 76-77 10.236498678467285 1.9240660132618654E-4 0.0 3.9082590894381645E-5 1.0221600695453661E-4 78-79 11.013221580372505 1.9841930761762988E-4 0.0 4.5095297185824975E-5 1.0221600695453661E-4 80-81 11.802168314168233 2.0743836705479487E-4 0.0 4.5095297185824975E-5 1.0221600695453661E-4 82-83 12.601097643584328 2.134510733462382E-4 0.0 4.5095297185824975E-5 1.0221600695453661E-4 84-85 13.408120075668705 2.1645742649195986E-4 0.0 4.5095297185824975E-5 1.0221600695453661E-4 86-87 14.232997239085524 2.1946377963768154E-4 0.0 4.5095297185824975E-5 1.0822871324597993E-4 88 14.858539660351841 2.2547648592912485E-4 0.0 4.5095297185824975E-5 1.0822871324597993E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTT 265470 0.0 37.578213 20-21 TACTTTT 277215 0.0 36.032333 22-23 GAGTACT 260585 0.0 34.40445 20-21 GTACTTT 267270 0.0 33.478306 22-23 ACTTTTT 295575 0.0 30.378408 24-25 ACGCGAG 2070 0.0 27.934006 14-15 AGAGTAC 377870 0.0 27.064777 18-19 CGCGAGT 1955 0.0 25.641027 16-17 GCGAGTA 2325 0.0 24.86981 16-17 GCTTGAA 22740 0.0 24.169619 60-61 CTTTTTT 428445 0.0 23.50056 24-25 GTATCAC 10610 0.0 23.293034 9 GTATAAC 6180 0.0 22.032307 9 GTTCAAC 8150 0.0 21.398315 9 TGCTTGA 26540 0.0 21.044992 58-59 CTTGAAA 26895 0.0 20.967026 60-61 GTGGTTC 9045 0.0 20.157366 6 ATAACGC 3875 0.0 19.314579 10-11 GTGTATC 17180 0.0 19.297987 6 AACGCGA 2675 0.0 18.827032 14-15 >>END_MODULE