FastQCFastQC Report
Thu 26 May 2016
SRR1660692_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660692_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42454759
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23384125.508009125667161No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5392271.2701214485754118Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3261750.7682884267462218No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960390.22621492210095928No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT456980.10763928727048008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA3042200.085.790335
GGTATCA3025600.085.7520758
TGGTATC3043650.085.040837
GTGGTAT3065400.084.858826
GTATCAA3059950.083.936199
GCAGTGG3162150.083.773413
AAGCAGT3219500.082.7806241
CAGTGGT3229450.081.2606354
AGCAGTG3311950.080.647882
CAACGCA2974200.045.1531812-13
ATCAACG2980350.045.067110-11
ACGCAGA3033250.044.5841114-15
AGAGTAC3065700.043.8524918-19
TCAACGC2974300.043.07211712-13
AACGCAG3003400.042.46056414-15
AGTACTT3156000.042.3432920-21
TATCAAC3043150.042.1227310-11
CGCAGAG3051450.041.68332316-17
GAGTACT3006350.041.5239820-21
GCAGAGT3295350.040.8611316-17
Produced by FastQC (version 0.11.5)