##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660692_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42454759 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19538325491378 34.0 31.0 34.0 30.0 34.0 2 32.343902387951374 34.0 31.0 34.0 30.0 34.0 3 32.31459844113118 34.0 31.0 34.0 30.0 34.0 4 35.72543122904078 37.0 35.0 37.0 35.0 37.0 5 35.75449977233412 37.0 35.0 37.0 35.0 37.0 6 35.7663778988829 37.0 35.0 37.0 35.0 37.0 7 35.70750464041028 37.0 35.0 37.0 35.0 37.0 8 35.75792624332174 37.0 35.0 37.0 35.0 37.0 9 37.446197845570154 39.0 38.0 39.0 35.0 39.0 10-11 37.3567532676372 39.0 37.0 39.0 34.5 39.0 12-13 37.40500684505122 39.0 38.0 39.0 35.0 39.0 14-15 38.8057258786936 40.0 38.5 41.0 35.0 41.0 16-17 38.744261779933794 40.0 38.0 41.0 35.0 41.0 18-19 38.579569324607405 40.0 38.0 41.0 34.0 41.0 20-21 38.49839136997574 40.0 38.0 41.0 34.0 41.0 22-23 38.36694444549785 40.0 38.0 41.0 34.0 41.0 24-25 38.34790592027622 40.0 38.0 41.0 34.0 41.0 26-27 38.332808884864946 40.0 38.0 41.0 34.0 41.0 28-29 38.22312105222409 40.0 38.0 41.0 33.5 41.0 30-31 38.05205344588106 40.0 38.0 41.0 33.0 41.0 32-33 37.926765383357846 40.0 38.0 41.0 33.0 41.0 34-35 37.85130631644853 40.0 38.0 41.0 33.0 41.0 36-37 37.676657026836494 40.0 37.5 41.0 32.5 41.0 38-39 37.368406142642336 40.0 36.0 41.0 32.5 41.0 40-41 37.42098625503915 40.0 36.0 41.0 32.5 41.0 42-43 37.25017281337058 40.0 35.5 41.0 31.5 41.0 44-45 37.13609825225954 40.0 35.0 41.0 31.0 41.0 46-47 37.192582379280495 40.0 35.0 41.0 31.5 41.0 48-49 37.19139321930905 40.0 35.0 41.0 31.5 41.0 50-51 35.808237187260914 38.5 34.0 40.0 29.0 40.5 52-53 35.87437749911618 38.5 35.0 39.5 28.5 40.5 54-55 36.34378949130297 39.0 35.0 41.0 28.5 41.0 56-57 36.00417401969 39.5 35.0 41.0 26.5 41.0 58-59 35.80476903661142 39.0 35.0 41.0 26.0 41.0 60-61 35.62803230139641 39.0 35.0 41.0 26.0 41.0 62-63 35.40929973245167 39.0 35.0 41.0 26.0 41.0 64-65 35.068360640087484 38.0 35.0 40.5 25.5 41.0 66-67 34.599804594344775 37.0 34.0 40.0 22.5 41.0 68-69 34.270538963605944 37.0 34.0 40.0 22.0 41.0 70-71 33.94051683346029 36.0 34.0 39.5 22.0 41.0 72-73 33.62924468609043 36.0 34.0 39.0 22.0 41.0 74-75 33.2573021907862 35.0 33.0 39.0 20.5 40.5 76-77 32.87129286259757 35.0 33.0 37.5 20.0 40.0 78-79 32.48617652028127 35.0 33.0 37.0 19.5 39.0 80-81 32.09068841964219 35.0 32.5 36.5 18.5 39.0 82-83 31.793838424568612 35.0 32.0 36.0 18.0 38.0 84-85 31.494832805434136 35.0 32.0 36.0 17.5 37.0 86-87 31.22711476232853 35.0 32.0 35.0 17.0 37.0 88-89 31.048242047493428 35.0 32.0 35.0 16.5 36.0 90-91 30.850028038081668 35.0 31.0 35.0 15.0 36.0 92-93 30.693106631461504 35.0 31.0 35.0 15.0 36.0 94-95 30.539691957737883 35.0 31.0 35.0 14.0 36.0 96-97 30.396527548301478 35.0 31.0 35.0 12.0 35.0 98-99 30.264984321310124 34.5 31.0 35.0 10.5 35.0 100 30.153381226354387 34.0 30.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.348196771240346 1101 2 0.3346691199534533 1101 3 0.3395424054337397 1101 4 0.2904780246689569 1101 5 0.2938209026088714 1101 6 0.21027330268226763 1101 7 0.25534866391053157 1101 8 0.2799644592849333 1101 9 0.35714761886058 1101 10-11 0.33218040462775633 1101 12-13 0.34075112368164895 1101 14-15 0.42219312037543943 1101 16-17 0.41068004070532993 1101 18-19 0.4726951954509033 1101 20-21 0.42988350663723196 1101 22-23 0.3931376470349335 1101 24-25 0.3987932393184508 1101 26-27 0.4211248269260395 1101 28-29 0.42367484774568 1101 30-31 0.4080852777952799 1101 32-33 0.3873479484537512 1101 34-35 0.405582468747717 1101 36-37 0.3336978150892662 1101 38-39 0.3707187552212403 1101 40-41 0.34370108991264203 1101 42-43 0.3197700303245483 1101 44-45 0.3238357752823333 1101 46-47 0.34166459195103016 1101 48-49 0.3269604152634784 1101 50-51 0.2515360720992561 1101 52-53 0.3430899094219555 1101 54-55 0.3974374574773165 1101 56-57 0.37608600148883653 1101 58-59 0.3810195720901888 1101 60-61 0.39017818454074416 1101 62-63 0.3728496020694507 1101 64-65 0.32583030693719905 1101 66-67 0.32549052628500164 1101 68-69 0.31121397570183973 1101 70-71 0.35288147666868497 1101 72-73 0.27924553494175086 1101 74-75 0.24834040679946412 1101 76-77 0.26062475756680215 1101 78-79 0.24576200303550877 1101 80-81 0.26912360427581206 1101 82-83 0.21328617785386328 1101 84-85 0.2199232578167205 1101 86-87 0.23994728647414476 1101 88-89 0.1806792989812145 1101 90-91 0.12223471290341337 1101 92-93 0.15898453571604776 1101 94-95 0.16731629008899773 1101 96-97 0.13398798314264582 1101 98-99 0.11188943689492703 1101 100 0.06474445919654315 1102 1 0.35630667978121977 1102 2 0.3175429563624945 1102 3 0.3337324034015765 1102 4 0.28431647001870886 1102 5 0.2848625725159195 1102 6 0.21019670945518953 1102 7 0.22876524168036383 1102 8 0.22744034406488112 1102 9 0.32059208963624997 1102 10-11 0.3193149508020454 1102 12-13 0.30221405291650427 1102 14-15 0.36176832524895985 1102 16-17 0.3914639239184865 1102 18-19 0.4213141700373555 1102 20-21 0.3891243419456245 1102 22-23 0.34156053698079347 1102 24-25 0.3315694106560372 1102 26-27 0.36929722664527276 1102 28-29 0.35188533876446826 1102 30-31 0.37617491196932207 1102 32-33 0.3223076678395387 1102 34-35 0.35049086732728796 1102 36-37 0.3247920413233061 1102 38-39 0.2953354401537851 1102 40-41 0.24228786204164976 1102 42-43 0.24111269883871245 1102 44-45 0.23342662069562436 1102 46-47 0.2753520696646703 1102 48-49 0.3079488061068574 1102 50-51 0.24128507180075331 1102 52-53 0.28098112469736947 1102 54-55 0.379292660753876 1102 56-57 0.3587754536732959 1102 58-59 0.3812199489380461 1102 60-61 0.34537916978712246 1102 62-63 0.3319747958904813 1102 64-65 0.29743829319102844 1102 66-67 0.321481335781975 1102 68-69 0.34925034032049496 1102 70-71 0.3621024235043748 1102 72-73 0.2647554827833858 1102 74-75 0.27502029601239286 1102 76-77 0.24954486774424112 1102 78-79 0.2742038296558533 1102 80-81 0.3124963791554549 1102 82-83 0.2536828307560377 1102 84-85 0.2456973568391838 1102 86-87 0.2672640874666463 1102 88-89 0.21754364984102992 1102 90-91 0.22336445745504463 1102 92-93 0.16381329663338917 1102 94-95 0.16617861321779515 1102 96-97 0.14432177939123036 1102 98-99 0.10512133992158113 1102 100 0.10632438745981077 1103 1 0.2560257487119202 1103 2 0.22461565095489533 1103 3 0.2464795690175805 1103 4 0.23557774296243394 1103 5 0.20945407141713446 1103 6 0.12246743294705453 1103 7 0.1519034083192068 1103 8 0.18807799377108836 1103 9 0.2348707700364585 1103 10-11 0.23760364447866067 1103 12-13 0.24052772732841987 1103 14-15 0.2823568565225969 1103 16-17 0.2730240060602753 1103 18-19 0.309304845562977 1103 20-21 0.2603328614160958 1103 22-23 0.26878384531099897 1103 24-25 0.24864035292012687 1103 26-27 0.25938013166153695 1103 28-29 0.2508988465607018 1103 30-31 0.21248792302284158 1103 32-33 0.2171538075991961 1103 34-35 0.23462670521762874 1103 36-37 0.18774304802344943 1103 38-39 0.19984963477406836 1103 40-41 0.1835103343511193 1103 42-43 0.12656430057413814 1103 44-45 0.1481053578722964 1103 46-47 0.2123063542826671 1103 48-49 0.1850778402719797 1103 50-51 0.16484197130468914 1103 52-53 0.2326380468025704 1103 54-55 0.29907249197582786 1103 56-57 0.29289742812587605 1103 58-59 0.2913126215196158 1103 60-61 0.27810388816782705 1103 62-63 0.2412643458323558 1103 64-65 0.26143184650150175 1103 66-67 0.2608002542943524 1103 68-69 0.2289499485635389 1103 70-71 0.28811542326452866 1103 72-73 0.22499940539680097 1103 74-75 0.2278730527189623 1103 76-77 0.20975697347682853 1103 78-79 0.23442826073557654 1103 80-81 0.2598419465351114 1103 82-83 0.2014006194074689 1103 84-85 0.211800457137862 1103 86-87 0.18915408651878707 1103 88-89 0.12815279687339398 1103 90-91 0.13755871423771637 1103 92-93 0.13339981058739525 1103 94-95 0.12149900622334542 1103 96-97 0.06150417186766077 1103 98-99 0.08502012923063518 1103 100 0.05679249156721866 1104 1 0.2115815255456326 1104 2 0.18165937022375545 1104 3 0.19966177723522094 1104 4 0.21231271572273158 1104 5 0.17118124767122112 1104 6 0.1292870880891428 1104 7 0.15198077726287806 1104 8 0.15249246366368396 1104 9 0.19840223672378698 1104 10-11 0.22503647175799557 1104 12-13 0.21285494041784148 1104 14-15 0.22316784625053998 1104 16-17 0.25311650800980345 1104 18-19 0.2967090662264411 1104 20-21 0.24150721708912926 1104 22-23 0.23293414796811618 1104 24-25 0.2341792075223239 1104 26-27 0.25794003439654034 1104 28-29 0.19658466919529616 1104 30-31 0.20811895184538542 1104 32-33 0.19713484328117659 1104 34-35 0.18952297432152676 1104 36-37 0.16878744266298895 1104 38-39 0.1910776488057664 1104 40-41 0.16697286371736908 1104 42-43 0.13433469533037368 1104 44-45 0.11367018616127211 1104 46-47 0.1313177796655154 1104 48-49 0.1547937369529251 1104 50-51 0.18054649691156754 1104 52-53 0.1892104537860675 1104 54-55 0.20614016639599697 1104 56-57 0.27162403827409065 1104 58-59 0.23578549331833898 1104 60-61 0.24726315582670821 1104 62-63 0.25364993425820614 1104 64-65 0.2510790628558581 1104 66-67 0.2548733973835482 1104 68-69 0.26185115406683934 1104 70-71 0.2578849250559614 1104 72-73 0.19316589716306964 1104 74-75 0.208470373462319 1104 76-77 0.20717345326769276 1104 78-79 0.2258905327374663 1104 80-81 0.26657182560992965 1104 82-83 0.19024626786037757 1104 84-85 0.19679902634475255 1104 86-87 0.20580223339993964 1104 88-89 0.18306099324184544 1104 90-91 0.17394139438864897 1104 92-93 0.14434471433223806 1104 94-95 0.13663654688144078 1104 96-97 0.1584731849278782 1104 98-99 0.1299423798535102 1104 100 0.15180894740130313 1105 1 0.130450672348978 1105 2 0.1223312714803555 1105 3 0.13889335705544426 1105 4 0.14695162448028753 1105 5 0.1191881110408346 1105 6 0.06257709858926574 1105 7 0.06821708398459236 1105 8 0.07026436012183268 1105 9 0.07816339785862425 1105 10-11 0.06866772758538531 1105 12-13 0.08672462035958262 1105 14-15 0.08852868474691888 1105 16-17 0.11064186770313 1105 18-19 0.13910794213050792 1105 20-21 0.1235390086539141 1105 22-23 0.10850473483125711 1105 24-25 0.10073853702765945 1105 26-27 0.12915281449622285 1105 28-29 0.09584296002986292 1105 30-31 0.09662797354972952 1105 32-33 0.11509860030748342 1105 34-35 0.11233837598491192 1105 36-37 0.09750131530122985 1105 38-39 0.10969875502093629 1105 40-41 0.10011698511247147 1105 42-43 0.11702187826391253 1105 44-45 0.0950971770118656 1105 46-47 0.13729619207069987 1105 48-49 0.11733945801958612 1105 50-51 0.08940550608085118 1105 52-53 0.14482309444107244 1105 54-55 0.20078491528446563 1105 56-57 0.19287655526571257 1105 58-59 0.19262358971408133 1105 60-61 0.12917090848996082 1105 62-63 0.1499983678779202 1105 64-65 0.11086055303751863 1105 66-67 0.14056411057349294 1105 68-69 0.13373147708935562 1105 70-71 0.17465969254670455 1105 72-73 0.16068835230136358 1105 74-75 0.17489788368810366 1105 76-77 0.1467931941532825 1105 78-79 0.12403697362492494 1105 80-81 0.15758751778255942 1105 82-83 0.11943897729074493 1105 84-85 0.1107270739381967 1105 86-87 0.12037573086963604 1105 88-89 0.07793639603452363 1105 90-91 0.13757604290465864 1105 92-93 0.1179577681409576 1105 94-95 0.08825353460228058 1105 96-97 0.07032664397643984 1105 98-99 0.0675192184899771 1105 100 0.04682841513720959 1106 1 0.09331536792598882 1106 2 0.08848825508700742 1106 3 0.10684378569023067 1106 4 0.09588923908961533 1106 5 0.077723473973343 1106 6 0.02771924152119709 1106 7 0.05999071632198394 1106 8 0.06898354149966224 1106 9 0.10863298452035508 1106 10-11 0.08664816997164593 1106 12-13 0.07496288226398207 1106 14-15 0.0964060129171358 1106 16-17 0.08980675433090113 1106 18-19 0.1637588708916411 1106 20-21 0.12649991744395095 1106 22-23 0.12330172249918547 1106 24-25 0.06985086914117744 1106 26-27 0.11927475139098931 1106 28-29 0.0926319332496206 1106 30-31 0.11062584164205447 1106 32-33 0.10804104862089758 1106 34-35 0.038552067005959145 1106 36-37 0.037471891618089614 1106 38-39 0.07439249684669136 1106 40-41 0.06432759420820844 1106 42-43 0.07862106083020848 1106 44-45 0.07728723049431352 1106 46-47 0.08817484494849026 1106 48-49 0.0948143772062835 1106 50-51 -9.572193237374904E-4 1106 52-53 0.059491465382791375 1106 54-55 0.14131317498577545 1106 56-57 0.17696353313442614 1106 58-59 0.18454131565187737 1106 60-61 0.1146158874494958 1106 62-63 0.1112095933973265 1106 64-65 0.05767964928395486 1106 66-67 0.11262624242268515 1106 68-69 0.12960982664436926 1106 70-71 0.19744443211957474 1106 72-73 0.16230687289315426 1106 74-75 0.1263310515010403 1106 76-77 0.05788727179429287 1106 78-79 0.10857224722097669 1106 80-81 0.17260081277472494 1106 82-83 0.17465938122155578 1106 84-85 0.19859855161724838 1106 86-87 0.15763730747261917 1106 88-89 0.14514203416980465 1106 90-91 0.1427518235041667 1106 92-93 0.13923717018823112 1106 94-95 0.1256316044065926 1106 96-97 0.11382191877270742 1106 98-99 0.10410607390228677 1106 100 0.122364363428769 1107 1 0.03610956193477932 1107 2 0.05065757015396599 1107 3 0.03443278362463076 1107 4 0.08353560457906184 1107 5 0.06536379275349447 1107 6 0.02693529124436367 1107 7 0.02043511929205266 1107 8 0.01707075753871834 1107 9 0.049784073551847996 1107 10-11 0.04660884356637496 1107 12-13 0.05238280748494617 1107 14-15 0.01953011793515458 1107 16-17 0.023349778973056345 1107 18-19 0.09239917444762824 1107 20-21 0.03452412068526911 1107 22-23 0.047475018292438165 1107 24-25 0.008624017058302513 1107 26-27 -0.0024069128546528873 1107 28-29 0.06261368881615681 1107 30-31 0.030497067568106218 1107 32-33 -0.01773004410990353 1107 34-35 0.01324994098580845 1107 36-37 -0.014144377169046152 1107 38-39 0.049859609843373676 1107 40-41 0.015548872833583971 1107 42-43 -0.023460253346279103 1107 44-45 0.02128028847758401 1107 46-47 0.04379880039136452 1107 48-49 0.00858781221315752 1107 50-51 -0.05433381417845595 1107 52-53 0.04163084295345243 1107 54-55 0.09667374633631454 1107 56-57 0.1480898096538965 1107 58-59 0.14613870661147388 1107 60-61 0.13066125703203824 1107 62-63 0.15592079816931204 1107 64-65 0.17834020858267507 1107 66-67 0.22477118543694985 1107 68-69 0.17315471079839995 1107 70-71 0.195814147990923 1107 72-73 0.14273026645872733 1107 74-75 0.14012490062694383 1107 76-77 0.15016365954549826 1107 78-79 0.1787078375555069 1107 80-81 0.21641867363315015 1107 82-83 0.13585377620803385 1107 84-85 0.19128474048243405 1107 86-87 0.193003157545391 1107 88-89 0.14678828803798183 1107 90-91 0.14883697975372812 1107 92-93 0.13844851527799307 1107 94-95 0.1426202039627782 1107 96-97 0.13641726164856394 1107 98-99 0.13460919198946542 1107 100 0.13030444707666433 1108 1 -0.04245665770497453 1108 2 -0.055046226216333594 1108 3 -0.015387254437754905 1108 4 0.0030944128795198367 1108 5 0.013740807842019365 1108 6 -0.03181987470298964 1108 7 -0.04266242568316159 1108 8 0.004680925864754215 1108 9 0.0013478354476035292 1108 10-11 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60897.0 15 82548.0 16 106674.0 17 133222.0 18 146514.0 19 165684.0 20 190029.0 21 232449.0 22 307615.0 23 448962.0 24 643266.0 25 799171.0 26 860349.0 27 814027.0 28 576147.0 29 559739.0 30 632024.0 31 761573.0 32 944428.0 33 1218142.0 34 1690558.0 35 2781640.0 36 4555079.0 37 8513057.0 38 1.1419846E7 39 3621123.0 40 8163.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.024801109203135 37.37755553995131 26.485838313823162 19.111805037022396 2 21.24213915289309 33.44502254543618 27.369418970502025 17.943419331168705 3 25.175185666169735 28.16124156305318 26.47179051817962 20.191782252597466 4 18.692137369957035 27.120659567217974 27.84825673518204 26.338946327642947 5 19.663020122659145 33.309335689639816 26.24238575041764 20.7852584372834 6 27.647988667876916 26.625653144535004 26.50871002357622 19.217648164011855 7 20.967102112012544 26.85113401776179 32.92562451546199 19.256139354763672 8 25.717462211801458 28.205672936586158 26.640961817975473 19.43590303363692 9 25.545955225130108 27.952074124113473 27.100187376594352 19.401783274162064 10-11 19.779624920189953 31.123143041665696 29.849043186933557 19.2481888512108 12-13 19.829690132303167 27.178844996608603 29.54745611206484 23.44400875902339 14-15 19.826190728431044 33.75703543968611 26.98393971334144 19.43283411854141 16-17 23.31418056227926 27.008700550852055 26.23506033485211 23.442058552016572 18-19 20.180301610277983 30.439647286797083 26.89231293771865 22.487738165206284 20-21 22.64188854333656 31.21020860654386 26.893575254864693 19.254327595254885 22-23 23.91812325734965 27.29015496095892 29.507228238432415 19.284493543259018 24-25 19.95394297584088 31.0422462828908 26.25200903292229 22.75180170834603 26-27 19.255461278502757 28.600202328012504 32.86850873667567 19.27582765680907 28-29 19.996091827219185 27.919199528946216 32.82941108500558 19.255297558829014 30-31 20.046083299406405 27.16535294418691 33.50034195869394 19.288221797712747 32-33 20.0337896593353 27.24203242155527 33.44249450707796 19.281683412031473 34-35 20.05819189186095 28.026733106088514 32.6530766078997 19.26199839415084 36-37 19.309157214680027 27.9974911046806 33.3867115119377 19.30664016870168 38-39 19.363544068147103 27.26528448189226 33.336949860492595 20.034221589468046 40-41 20.169791761611407 27.33138833107484 32.535920414695305 19.96289949261845 42-43 20.20626924941895 27.374856280113804 33.19041634056237 19.228458129904872 44-45 20.97333430617062 27.442994355482636 32.388771926601194 19.19489941174555 46-47 19.53247414014187 27.535071093815098 33.02328219022715 19.909172575815884 48-49 20.325853560226257 27.542585764229955 32.169195365412335 19.962365310131457 50-51 20.429405925351123 27.59736062716621 32.05565166406591 19.917581783416757 52-53 19.80269223031921 28.498125088249655 32.548496487320676 19.15068619411046 54-55 20.155657139497592 27.997096619842903 31.8075810958205 20.03966514483901 56-57 20.588054578265684 29.224960007914504 30.78550550791053 19.401479905909287 58-59 20.825584497068757 29.675424432419817 30.159166655349484 19.339824415161942 60-61 21.006614619548323 30.624602055398185 29.063336930852856 19.305446394200633 62-63 21.180650105804194 30.72335948365701 28.856766317469884 19.23922409306891 64-65 21.366098527828154 30.732347723767262 28.76254473680965 19.139009011594933 66-67 21.57663201730096 30.453115874829813 28.8700243698852 19.100227737984028 68-69 21.778792944256143 30.075045365737818 29.095867149102762 19.05029454090328 70-71 22.01119434568981 29.799396137124777 29.209040872824627 18.980368644360787 72-73 22.246199540712716 29.59799007284839 29.213932788332276 18.941877598106622 74-75 22.498967306004573 29.561176245821613 29.069276601654387 18.870579846519426 76-77 22.820559797238403 29.592303167793112 28.779689676353055 18.807447358615438 78-79 23.157499864145123 29.634058190266032 28.43920540713487 18.769236538453974 80-81 23.471530595182223 29.72304990148941 28.09374814383892 18.711671359489447 82-83 23.678637133331435 29.81129144678026 27.79824183372117 18.711829586167138 84-85 23.892856481177812 29.781032398412886 27.611638347315736 18.71447277309356 86-87 24.108582753950923 29.72101569980547 27.49034286745481 18.6800586787888 88-89 24.324535492039324 29.66486613148918 27.37584190557038 18.634756470901117 90-91 24.5614771494872 29.576659419583457 27.22858585136586 18.633277579563483 92-93 24.722307766488132 29.517646466897762 27.172850657330933 18.587195109283172 94-95 24.876499581064163 29.509189941530828 27.087883199739593 18.526427277665412 96-97 25.007705530374274 29.464954117202975 26.987144101367917 18.540196251054837 98-99 25.12544601118791 29.468529440215907 26.895181468506262 18.510843080089916 100 25.184509290448258 29.51895402590803 26.79554530767002 18.50099137597369 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 299801.0 1 174831.0 2 39295.0 3 26877.5 4 24748.5 5 24454.5 6 24347.5 7 23925.0 8 23395.0 9 23304.5 10 24561.5 11 35793.0 12 302859.0 13 345879.5 14 120300.0 15 113955.5 16 125964.0 17 138447.5 18 150641.0 19 164383.0 20 180348.5 21 204699.5 22 239681.5 23 281820.0 24 327662.5 25 377964.5 26 435904.0 27 504861.5 28 588355.0 29 674961.5 30 750802.5 31 821937.5 32 897659.5 33 961195.0 34 1015556.0 35 1081664.5 36 1149918.5 37 1215780.5 38 1285933.5 39 1333999.0 40 1359909.0 41 1401917.0 42 1453974.0 43 1467975.5 44 1462937.5 45 1472990.5 46 1479080.5 47 1476115.5 48 1449543.5 49 1401869.5 50 1353119.5 51 1301358.5 52 1237374.5 53 1149587.0 54 1046745.5 55 944842.5 56 846054.5 57 748054.5 58 646101.0 59 540585.5 60 438903.0 61 350171.5 62 271413.5 63 200021.0 64 143391.5 65 103260.5 66 75418.0 67 56433.5 68 41966.5 69 31656.0 70 24439.0 71 19184.0 72 15432.5 73 12565.0 74 10370.0 75 8841.0 76 6881.0 77 4348.5 78 2755.5 79 1943.5 80 1405.5 81 1048.0 82 819.5 83 651.5 84 511.5 85 422.0 86 334.5 87 255.5 88 213.5 89 183.5 90 156.0 91 121.0 92 109.0 93 103.0 94 84.0 95 70.0 96 59.5 97 45.5 98 32.5 99 22.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004569570162911536 2 1.1070608126641349E-4 3 0.08928092136855612 4 0.025452976897124772 5 0.017750660179227492 6 0.05623633383480048 7 0.03801222849951875 8 0.025641412780131434 9 0.005382199908377763 10-11 0.007491504073783578 12-13 0.014582581896177999 14-15 0.02670725324338786 16-17 0.0493501800351758 18-19 0.06233341237433476 20-21 0.04511508356460109 22-23 0.02667898786093686 24-25 0.018915429481062417 26-27 0.017311369026968216 28-29 0.01237552661646248 30-31 0.010497056407739826 32-33 0.00779653465940061 34-35 0.005777915262691752 36-37 0.009901127927731259 38-39 0.014408278704396838 40-41 0.016068869923393042 42-43 0.009720936114606138 44-45 0.0038075825610033496 46-47 0.002759407961778796 48-49 0.00198328766864511 50-51 0.0010151983196983877 52-53 0.0014780439573334994 54-55 2.4732209644624293E-4 56-57 0.002464976894580888 58-59 0.010758511195411567 60-61 0.021928048160631416 62-63 0.027084125009401182 64-65 0.009041389211513366 66-67 0.0021846785186084793 68-69 0.0015039538912469154 70-71 0.00215641313615748 72-73 6.713028332112308E-4 74-75 6.265493109971487E-4 76-77 0.0013331838722721286 78-79 0.0032269644964890744 80-81 0.007609276500662741 82-83 0.008876507813882538 84-85 0.007656385471414406 86-87 0.004569570162911536 88-89 0.0023377826735513914 90-91 0.0026557682261251323 92-93 0.002602770634029509 94-95 0.0032045877353820335 96-97 0.0034954856297735666 98-99 0.007842465905883484 100 0.013371881347860201 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.2454759E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.97394656533641 #Duplication Level Percentage of deduplicated Percentage of total 1 64.01584681454906 21.748709590122512 2 16.713367061417518 11.35638078942905 3 7.020133132201881 7.1550488374492325 4 3.505181447213653 4.763393887577808 5 2.075989439094298 3.5264777136996197 6 1.3494649025579188 2.7507989094779783 7 0.8602114254121558 2.045734390129111 8 0.6862390453215522 1.8651398925441511 9 0.5010622074043535 1.5320754594238513 >10 2.91531739747743 17.73538904750371 >50 0.1815616473383488 4.233787620070513 >100 0.17219064911980705 11.884442616249368 >500 0.0025328848890449817 0.5283059443418359 >1k 7.083351714562753E-4 0.560517052889618 >5k 1.1754944240609142E-4 0.2613846686949423 >10k+ 7.606138905649327E-5 8.052413580396944 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2338412 5.508009125667161 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 539227 1.2701214485754118 Illumina Single End PCR Primer 1 (100% over 50bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 326175 0.7682884267462218 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96039 0.22621492210095928 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 45698 0.10763928727048008 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4778578768990303 7.066345612749798E-6 0.0 0.0 7.537435320266452E-5 2 1.4779096967668572 7.066345612749798E-6 0.0 0.0 7.537435320266452E-5 3 1.4779473839434585 9.421794150333065E-6 0.0 0.0 7.772980174024777E-5 4 1.4779709384288344 1.177724268791633E-5 0.0 2.355448537583266E-6 7.772980174024777E-5 5 1.477989782017135 1.4132691225499596E-5 0.0 2.355448537583266E-6 7.772980174024777E-5 6 1.4779992038112852 1.4132691225499596E-5 0.0 2.355448537583266E-6 7.772980174024777E-5 7 1.4780156919510483 1.4132691225499596E-5 0.0 2.355448537583266E-6 7.772980174024777E-5 8 1.4780439573334994 1.4132691225499596E-5 0.0 2.355448537583266E-6 7.772980174024777E-5 9 1.4780839999586384 1.4132691225499596E-5 0.0 2.355448537583266E-6 7.772980174024777E-5 10-11 1.4781511302419594 1.4132691225499596E-5 0.0 2.355448537583266E-6 7.772980174024777E-5 12-13 1.4782453481834628 1.4132691225499596E-5 0.0 2.355448537583266E-6 8.008525027783104E-5 14-15 1.4783442770220412 1.4132691225499596E-5 0.0 5.888621343958165E-6 8.126297454662268E-5 16-17 1.4784208290995127 1.4132691225499596E-5 0.0 7.066345612749798E-6 8.715159589058085E-5 18-19 1.4785433124234673 1.4132691225499596E-5 0.0 9.421794150333065E-6 1.001065628472888E-4 20-21 1.4786516630561959 1.6488139763082864E-5 0.0 9.421794150333065E-6 1.1423925407278839E-4 22-23 1.4788648311488473 2.002131256945776E-5 0.0 9.421794150333065E-6 1.2719422102949637E-4 24-25 1.4795149349452201 2.1199036838249395E-5 0.0 9.421794150333065E-6 1.319051181046629E-4 26-27 1.4815429761360792 2.5909933913415927E-5 0.0 9.421794150333065E-6 1.3661601517982942E-4 28-29 1.4877837370364062 3.297627952616572E-5 0.0 9.421794150333065E-6 1.3661601517982942E-4 30-31 1.5035805526537085 3.297627952616572E-5 0.0 9.421794150333065E-6 1.4368236079257923E-4 32-33 1.5368524409713409 4.004262513891552E-5 0.0 9.421794150333065E-6 1.4839325786774577E-4 34-35 1.5952616760820617 4.239807367649879E-5 0.0 9.421794150333065E-6 1.4839325786774577E-4 36-37 1.6859028689810724 4.239807367649879E-5 0.0 1.177724268791633E-5 1.5192643067412064E-4 38-39 1.8146057547988907 4.593124648287369E-5 0.0 1.177724268791633E-5 1.5781505201807882E-4 40-41 1.9863085313945605 5.064214355804022E-5 0.0 1.177724268791633E-5 1.672368461684119E-4 42-43 2.2027130103364856 5.888621343958165E-5 0.0 1.177724268791633E-5 1.6959229470599516E-4 44-45 2.4668200330615466 6.359711051474819E-5 0.0 1.177724268791633E-5 1.7194774324357843E-4 46-47 2.7793115019213745 6.713028332112308E-5 0.0 2.1199036838249395E-5 1.7430319178116167E-4 48-49 3.141631306869508 7.301890466508125E-5 0.0 2.355448537583266E-5 1.7430319178116167E-4 50-51 3.553547436224994 7.655207747145614E-5 0.0 2.5909933913415927E-5 1.754809160499533E-4 52-53 4.008880370749484 7.890752600903941E-5 0.0 2.5909933913415927E-5 1.7665864031874494E-4 54-55 4.509627530802849 8.479614735299758E-5 0.0 2.5909933913415927E-5 1.7665864031874494E-4 56-57 5.054776073513926 8.479614735299758E-5 0.0 2.5909933913415927E-5 1.8608043446907802E-4 58-59 5.6455873415745925 8.950704442816412E-5 0.0 2.8265382450999192E-5 1.9196905581303616E-4 60-61 6.2716973614194815 9.421794150333064E-5 0.0 2.8265382450999192E-5 1.9550222861941107E-4 62-63 6.929366858495181 1.001065628472888E-4 0.0 2.9443106719790824E-5 2.0256857423216087E-4 64-65 7.62012451890258 1.0481745992245534E-4 0.0 3.062083098858246E-5 2.0492402276974414E-4 66-67 8.342128381885292 1.1188380553520513E-4 0.0 3.297627952616572E-5 2.072794713073274E-4 68-69 9.093590662003287 1.1541697834158004E-4 0.0 3.297627952616572E-5 2.1081264411370232E-4 70-71 9.870432900113743 1.177724268791633E-4 0.0 3.533172806374899E-5 2.1670126545766046E-4 72-73 10.666802984325031 1.2012787541674656E-4 0.0 3.768717660133226E-5 2.1670126545766046E-4 74-75 11.484114183759704 1.2012787541674656E-4 0.0 4.004262513891552E-5 2.2023443826403537E-4 76-77 12.321486738388977 1.3072739383587127E-4 0.0 4.239807367649879E-5 2.2376761107041027E-4 78-79 13.177164661328074 1.3426056664224615E-4 0.0 4.475352221408205E-5 2.2612305960799354E-4 80-81 14.048120023481939 1.3426056664224615E-4 1.177724268791633E-6 4.593124648287369E-5 2.2730078387678517E-4 82-83 14.936296069894073 1.3661601517982942E-4 2.355448537583266E-6 4.9464419289248584E-5 2.320116809519517E-4 84-85 15.83893457032697 1.4839325786774577E-4 2.355448537583266E-6 4.9464419289248584E-5 2.355448537583266E-4 86-87 16.752936932229435 1.5899277628687045E-4 2.355448537583266E-6 5.064214355804022E-5 2.3672257802711822E-4 88 17.44435058505455 1.6959229470599516E-4 2.355448537583266E-6 5.181986782683185E-5 2.4496664790865966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 304220 0.0 85.79033 5 GGTATCA 302560 0.0 85.752075 8 TGGTATC 304365 0.0 85.04083 7 GTGGTAT 306540 0.0 84.85882 6 GTATCAA 305995 0.0 83.93619 9 GCAGTGG 316215 0.0 83.77341 3 AAGCAGT 321950 0.0 82.780624 1 CAGTGGT 322945 0.0 81.260635 4 AGCAGTG 331195 0.0 80.64788 2 CAACGCA 297420 0.0 45.15318 12-13 ATCAACG 298035 0.0 45.0671 10-11 ACGCAGA 303325 0.0 44.58411 14-15 AGAGTAC 306570 0.0 43.85249 18-19 TCAACGC 297430 0.0 43.072117 12-13 AACGCAG 300340 0.0 42.460564 14-15 AGTACTT 315600 0.0 42.34329 20-21 TATCAAC 304315 0.0 42.12273 10-11 CGCAGAG 305145 0.0 41.683323 16-17 GAGTACT 300635 0.0 41.52398 20-21 GCAGAGT 329535 0.0 40.86113 16-17 >>END_MODULE