FastQCFastQC Report
Thu 26 May 2016
SRR1660692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42454759
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22403105.276934913233166No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG3361380.7917557605261638TruSeq Adapter, Index 5 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2975590.7008849113947391No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACAAACATCTCGTATG2232540.5258633078096144TruSeq Adapter, Index 5 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080520.25451092538294706No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT483440.11387180410092541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2910250.084.761578
AGTGGTA2945550.084.422625
TGGTATC2943000.083.7215047
GTGGTAT2966950.083.5060966
GTATCAA2950750.082.854019
GCAGTGG3049950.082.5632553
AAGCAGT3091550.081.4804841
CAGTGGT3131050.079.980974
AGCAGTG3215050.078.886212
CAACGCA2858050.044.5963112-13
ATCAACG2860550.044.495410-11
ACGCAGA2926550.043.97221414-15
AGAGTAC2977350.043.17944718-19
TCAACGC2859050.042.5773312-13
AACGCAG2886400.041.99373614-15
AGTACTT3063900.041.8240220-21
TATCAAC2938150.041.50486810-11
CGCAGAG2936050.041.28029616-17
GAGTACT2916200.041.11146520-21
GCAGAGT3200600.040.32201416-17
Produced by FastQC (version 0.11.5)