##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660692_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42454759 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.273734235542356 34.0 31.0 34.0 30.0 34.0 2 32.637524429240074 34.0 31.0 34.0 31.0 34.0 3 32.79126285936519 34.0 31.0 34.0 31.0 34.0 4 36.23429227333501 37.0 37.0 37.0 35.0 37.0 5 36.15921904067339 37.0 37.0 37.0 35.0 37.0 6 36.16690190138637 37.0 37.0 37.0 35.0 37.0 7 36.08281669906547 37.0 35.0 37.0 35.0 37.0 8 36.11162423510636 37.0 36.0 37.0 35.0 37.0 9 37.8735756808795 39.0 38.0 39.0 35.0 39.0 10-11 37.77474974007036 39.0 37.5 39.0 35.0 39.0 12-13 37.82675699560561 39.0 38.0 39.0 35.0 39.0 14-15 39.31528155653881 41.0 39.0 41.0 36.0 41.0 16-17 39.25357001791012 41.0 39.0 41.0 36.0 41.0 18-19 39.17524225022689 41.0 39.0 41.0 36.0 41.0 20-21 39.142846153007255 40.5 39.0 41.0 35.5 41.0 22-23 38.99819935381096 40.0 38.5 41.0 35.0 41.0 24-25 38.97871359957549 40.0 39.0 41.0 35.0 41.0 26-27 38.92509952771137 40.0 38.5 41.0 35.0 41.0 28-29 38.82364337529275 40.0 38.0 41.0 35.0 41.0 30-31 38.69287689278839 40.0 38.0 41.0 35.0 41.0 32-33 38.55523425536346 40.0 38.0 41.0 34.0 41.0 34-35 38.452635945948956 40.0 38.0 41.0 34.0 41.0 36-37 38.30447157879285 40.0 38.0 41.0 34.0 41.0 38-39 37.88720116159416 40.0 37.0 41.0 33.5 41.0 40-41 37.74715869898119 40.0 36.0 41.0 33.0 41.0 42-43 37.65736327698857 40.0 36.5 41.0 33.0 41.0 44-45 37.769073097317545 40.0 36.0 41.0 33.0 41.0 46-47 37.66000104958787 40.0 36.0 41.0 33.0 41.0 48-49 37.55126358154571 40.0 36.0 41.0 33.0 41.0 50-51 37.38850834131458 40.0 35.5 41.0 33.0 41.0 52-53 36.977949704060265 40.0 35.0 41.0 31.5 41.0 54-55 36.49416882804587 39.0 35.0 41.0 30.0 41.0 56-57 36.16346749253717 39.0 35.0 41.0 28.0 41.0 58-59 35.94603902945251 39.0 35.0 41.0 27.5 41.0 60-61 35.76959613644256 39.0 35.0 41.0 27.0 41.0 62-63 35.579557476701254 39.0 35.0 41.0 27.0 41.0 64-65 35.28462918609431 38.0 35.0 40.0 26.5 41.0 66-67 34.99828816599807 37.0 35.0 40.0 26.0 41.0 68-69 34.70998280781667 37.0 34.0 40.0 26.0 41.0 70-71 34.25828125652533 36.0 34.0 39.0 25.0 41.0 72-73 32.489801261149545 35.5 33.0 39.0 12.0 41.0 74-75 31.58586420193788 35.0 30.5 37.5 9.5 40.0 76-77 31.22662699604537 34.0 30.0 36.0 19.0 39.0 78-79 32.53219469459243 35.0 33.0 37.0 20.5 39.0 80-81 32.545140286863955 35.0 33.0 36.5 21.0 39.0 82-83 32.30135132789236 35.0 33.0 36.0 20.0 38.0 84-85 32.06378416846036 35.0 33.0 36.0 20.0 37.0 86-87 31.778802630819314 35.0 33.0 35.0 19.5 37.0 88-89 31.593447215187346 35.0 33.0 35.0 19.0 36.0 90-91 31.47926622313414 35.0 33.0 35.0 19.0 36.0 92-93 31.358670673410252 35.0 33.0 35.0 19.0 36.0 94-95 31.239161162120837 35.0 33.0 35.0 18.0 36.0 96-97 31.113706581163257 35.0 33.0 35.0 18.0 35.0 98-99 31.021577875403793 35.0 32.0 35.0 17.0 35.0 100 30.96305945347611 35.0 32.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5753225401274378 1101 2 0.4030172016107656 1101 3 0.31863712496001995 1101 4 0.23415981211299197 1101 5 0.21349257389177723 1101 6 0.22266979540096088 1101 7 0.2514250003339882 1101 8 0.2636696626308108 1101 9 0.3086105938144712 1101 10-11 0.31870623848880086 1101 12-13 0.3079077315946108 1101 14-15 0.33923524639343583 1101 16-17 0.39232530739355553 1101 18-19 0.4161009042923709 1101 20-21 0.376994236448148 1101 22-23 0.37289729068165656 1101 24-25 0.3735278352129612 1101 26-27 0.3790687403480959 1101 28-29 0.36977449104804805 1101 30-31 0.34961698221547977 1101 32-33 0.4300355478015945 1101 34-35 0.3702585057588621 1101 36-37 0.3571179128287909 1101 38-39 0.38393516547542106 1101 40-41 0.36996920609917794 1101 42-43 0.35661219902875274 1101 44-45 0.36242495013772924 1101 46-47 0.30445869198460684 1101 48-49 0.289960655637195 1101 50-51 0.26696762958449227 1101 52-53 0.1733605948878605 1101 54-55 0.1327395051857181 1101 56-57 0.1461785789901242 1101 58-59 0.19498768209815154 1101 60-61 0.13243674758966506 1101 62-63 0.15349589516063844 1101 64-65 0.17716486304436074 1101 66-67 0.14946474432393586 1101 68-69 0.16921369578782475 1101 70-71 0.18502500695159085 1101 72-73 1.4503489343989813 1101 74-75 1.9942599330901452 1101 76-77 0.6502357540376202 1101 78-79 0.370651868735564 1101 80-81 0.2713379002287226 1101 82-83 0.244429388720242 1101 84-85 0.20655098954112106 1101 86-87 0.23961957494573127 1101 88-89 0.22092509768483737 1101 90-91 0.1731417102383226 1101 92-93 0.12939428614692616 1101 94-95 0.10292324075666315 1101 96-97 0.13684359567109894 1101 98-99 0.12742752652886224 1101 100 0.1093503873146382 1102 1 0.5267129333478806 1102 2 0.38641203207895103 1102 3 0.3071223206189657 1102 4 0.22046552115889284 1102 5 0.19228276708804515 1102 6 0.2027149057943376 1102 7 0.22303720443946418 1102 8 0.23267171774473638 1102 9 0.2776216255399575 1102 10-11 0.2625111190936167 1102 12-13 0.27955691818392836 1102 14-15 0.34186412298532076 1102 16-17 0.3419131731265779 1102 18-19 0.3585877058725302 1102 20-21 0.32483755551392335 1102 22-23 0.30156618720233297 1102 24-25 0.3394761106426145 1102 26-27 0.31492136752360267 1102 28-29 0.3354275195593033 1102 30-31 0.3045719200800505 1102 32-33 0.35278989520115545 1102 34-35 0.3421670736581319 1102 36-37 0.3204880907785679 1102 38-39 0.3605198957481548 1102 40-41 0.3652711615976827 1102 42-43 0.31369843412754506 1102 44-45 0.34919856679484695 1102 46-47 0.30225329956406455 1102 48-49 0.24005490551247277 1102 50-51 0.21898762461766097 1102 52-53 0.11611844737299037 1102 54-55 0.1268030099830142 1102 56-57 0.17485514246169487 1102 58-59 0.2505771337156588 1102 60-61 0.1706914650097744 1102 62-63 0.16224959279838203 1102 64-65 0.12975100218125135 1102 66-67 0.13773500985168852 1102 68-69 0.13549193335106935 1102 70-71 0.18745910571260538 1102 72-73 1.466148439944405 1102 74-75 1.929757196247646 1102 76-77 0.5755441258023168 1102 78-79 0.34309861300724265 1102 80-81 0.2418615769154897 1102 82-83 0.2060058767144639 1102 84-85 0.17201458535063097 1102 86-87 0.19727662005993452 1102 88-89 0.1575779535662214 1102 90-91 0.06873787414216181 1102 92-93 0.05094927194337373 1102 94-95 0.07808537766307211 1102 96-97 0.0939579822713128 1102 98-99 0.05460360407140996 1102 100 0.06008586698933982 1103 1 0.4856763356091136 1103 2 0.34866274107758954 1103 3 0.270137294084428 1103 4 0.18610317658700382 1103 5 0.18928939671610578 1103 6 0.1792992005700924 1103 7 0.20431036071987307 1103 8 0.1904056074500815 1103 9 0.20548763675084558 1103 10-11 0.22629287887877325 1103 12-13 0.24683876576992958 1103 14-15 0.26120893495059505 1103 16-17 0.29447753094247986 1103 18-19 0.30958461340851073 1103 20-21 0.2790436831147787 1103 22-23 0.27098616374388484 1103 24-25 0.28396997943375624 1103 26-27 0.25143803551443966 1103 28-29 0.30027973726756585 1103 30-31 0.23657153786794538 1103 32-33 0.3077246850672424 1103 34-35 0.3015288959966185 1103 36-37 0.286273661990343 1103 38-39 0.3146744368263157 1103 40-41 0.3022716746179199 1103 42-43 0.2812248215673421 1103 44-45 0.3055457946791975 1103 46-47 0.2439782044035823 1103 48-49 0.19315309448779772 1103 50-51 0.21293166761720528 1103 52-53 0.0811794749344088 1103 54-55 0.05475600224473709 1103 56-57 0.1180314771004447 1103 58-59 0.20837411766734704 1103 60-61 0.16436703633093686 1103 62-63 0.1400701981681962 1103 64-65 0.11087917552100635 1103 66-67 0.1189985579670676 1103 68-69 0.12979791270328178 1103 70-71 0.14412378716623664 1103 72-73 1.443260755379562 1103 74-75 1.9035028279381159 1103 76-77 0.4927726784246751 1103 78-79 0.3037705230249941 1103 80-81 0.18962621626339882 1103 82-83 0.1631786545197329 1103 84-85 0.1466430746706422 1103 86-87 0.22691678823814598 1103 88-89 0.19216017713180733 1103 90-91 0.15100542583584797 1103 92-93 0.12377403856647717 1103 94-95 0.12402496131223373 1103 96-97 0.10023684260096744 1103 98-99 0.10172083709787216 1103 100 0.11830924710194779 1104 1 0.43223449685688564 1104 2 0.2979132189146796 1104 3 0.20224093808104016 1104 4 0.13631872318160276 1104 5 0.1389995506984576 1104 6 0.12975944944597018 1104 7 0.17174281091076438 1104 8 0.17503350015643093 1104 9 0.2085627038232829 1104 10-11 0.1931245078707704 1104 12-13 0.20251850301940522 1104 14-15 0.22724572277034127 1104 16-17 0.26616176553596205 1104 18-19 0.28432050341891113 1104 20-21 0.23865450961829993 1104 22-23 0.22118368468949967 1104 24-25 0.25066161100897233 1104 26-27 0.2326523980252091 1104 28-29 0.2419821743059387 1104 30-31 0.23127470162695118 1104 32-33 0.2917369372381273 1104 34-35 0.2577810443143065 1104 36-37 0.24527309856592439 1104 38-39 0.2687695187613599 1104 40-41 0.26679447857254246 1104 42-43 0.2480976995371975 1104 44-45 0.2277114638781228 1104 46-47 0.2054740230540304 1104 48-49 0.21306682023580237 1104 50-51 0.18573416620861138 1104 52-53 0.12459286594503283 1104 54-55 0.11259420588501712 1104 56-57 0.15952151715465845 1104 58-59 0.23499837243563348 1104 60-61 0.1805098817037134 1104 62-63 0.1905596364527824 1104 64-65 0.1939911407223036 1104 66-67 0.15372005623078877 1104 68-69 0.21220412884068907 1104 70-71 0.18380935525934206 1104 72-73 1.4592913736994788 1104 74-75 1.9320233214223883 1104 76-77 0.5677653693105817 1104 78-79 0.3627784303570323 1104 80-81 0.24041241317392092 1104 82-83 0.22293779078453468 1104 84-85 0.20919386405958562 1104 86-87 0.2675360343657296 1104 88-89 0.2687134793885484 1104 90-91 0.18515590542485327 1104 92-93 0.14541790979333769 1104 94-95 0.1522277912312937 1104 96-97 0.1228341532440318 1104 98-99 0.1423593890878152 1104 100 0.1392705511211112 1105 1 0.38211771751372936 1105 2 0.25293168522865983 1105 3 0.17743614190505497 1105 4 0.14419120749077763 1105 5 0.12027033665775377 1105 6 0.12221743487799586 1105 7 0.11905234302263779 1105 8 0.12398955715488569 1105 9 0.13034066582374493 1105 10-11 0.161825709295897 1105 12-13 0.14925235881260335 1105 14-15 0.16031667364269708 1105 16-17 0.18797313044915853 1105 18-19 0.2138041278624172 1105 20-21 0.18054883549203993 1105 22-23 0.14295344647718622 1105 24-25 0.2118443148564353 1105 26-27 0.16947692950732574 1105 28-29 0.20094565603393733 1105 30-31 0.17637659933588168 1105 32-33 0.24518943634967627 1105 34-35 0.21655614633058207 1105 36-37 0.188012157052718 1105 38-39 0.20201587174629765 1105 40-41 0.21396249180933324 1105 42-43 0.18746418375375384 1105 44-45 0.18262401085610236 1105 46-47 0.1625470133947644 1105 48-49 0.1303305526534686 1105 50-51 0.10865460981897712 1105 52-53 0.12019242349976622 1105 54-55 0.047361959945497745 1105 56-57 0.0338592633233219 1105 58-59 0.10351157632732821 1105 60-61 0.0642004062253747 1105 62-63 0.052782919514648086 1105 64-65 0.034284999208452405 1105 66-67 0.04142772593301913 1105 68-69 0.0766258368745909 1105 70-71 0.0721114481379459 1105 72-73 1.3762982235165495 1105 74-75 1.8501324717753 1105 76-77 0.45149243036730624 1105 78-79 0.25383205588570945 1105 80-81 0.14750128936507423 1105 82-83 0.11081545949775062 1105 84-85 0.10036251175649369 1105 86-87 0.2035767575261822 1105 88-89 0.16401161401395115 1105 90-91 0.11566837540807029 1105 92-93 0.07879288794549666 1105 94-95 0.09127501149717787 1105 96-97 0.0675087461005468 1105 98-99 0.050982159844316755 1105 100 0.06764909620970982 1106 1 0.36166333344014134 1106 2 0.23424811010129076 1106 3 0.1512800031355681 1106 4 0.10794892049747773 1106 5 0.10946942948520189 1106 6 0.10278389541604582 1106 7 0.08375009898497865 1106 8 0.10590705403983947 1106 9 0.10424968305282079 1106 10-11 0.11031818688629613 1106 12-13 0.13427947438934495 1106 14-15 0.0963206498570699 1106 16-17 0.11624152984989422 1106 18-19 0.1693380510030309 1106 20-21 0.11378516681192963 1106 22-23 0.08761485088619736 1106 24-25 0.1262958090740156 1106 26-27 0.11885046624362872 1106 28-29 0.12630574662232874 1106 30-31 0.10351994685592558 1106 32-33 0.17107564272028242 1106 34-35 0.1696937193703647 1106 36-37 0.11848884518519753 1106 38-39 0.1664212076437508 1106 40-41 0.16046864391331184 1106 42-43 0.1815004555060824 1106 44-45 0.1869938541351246 1106 46-47 0.11158409588225737 1106 48-49 0.088694102924606 1106 50-51 0.054271859581000115 1106 52-53 0.052696110691876186 1106 54-55 -0.002292535003618923 1106 56-57 -0.031127302903257714 1106 58-59 0.06183386237245969 1106 60-61 0.03542912255903019 1106 62-63 0.06853972682304033 1106 64-65 0.07678464681367103 1106 66-67 0.08808494399695377 1106 68-69 0.13036850279335255 1106 70-71 0.14565324899990628 1106 72-73 1.4658518059588275 1106 74-75 1.9640856996225757 1106 76-77 0.480264475594975 1106 78-79 0.334050299240495 1106 80-81 0.1927858037770207 1106 82-83 0.16875447882818406 1106 84-85 0.1327550823701884 1106 86-87 0.2127799754656401 1106 88-89 0.2111971705565061 1106 90-91 0.15092587063391605 1106 92-93 0.10831002705744197 1106 94-95 0.1072839345659311 1106 96-97 0.18642338123465052 1106 98-99 0.1837139667080372 1106 100 0.1623213518934321 1107 1 0.3240559567853438 1107 2 0.21240048060328576 1107 3 0.12480350970971443 1107 4 0.08656131924671229 1107 5 0.08380919930678488 1107 6 0.0687960798486813 1107 7 0.07728712797113246 1107 8 0.07292164503783738 1107 9 0.10379726065793449 1107 10-11 0.08381736691816855 1107 12-13 0.0968513167230256 1107 14-15 0.08332579580333999 1107 16-17 0.11466437673718133 1107 18-19 0.12013419195360342 1107 20-21 0.0801183921750308 1107 22-23 0.07831028809604135 1107 24-25 0.08618208332061528 1107 26-27 0.08402171403248104 1107 28-29 0.10526327735758656 1107 30-31 0.11743446039221794 1107 32-33 0.11480998667258291 1107 34-35 0.08121396879435139 1107 36-37 0.10218694187823729 1107 38-39 0.11788018757442842 1107 40-41 0.1408479739764843 1107 42-43 0.08872152324644134 1107 44-45 0.1192443817730009 1107 46-47 0.10966316306099344 1107 48-49 0.097836767893142 1107 50-51 0.06371687163248652 1107 52-53 0.05713511367226687 1107 54-55 0.049309209552660604 1107 56-57 0.03016927421575133 1107 58-59 0.14550054032095971 1107 60-61 0.0588437520369709 1107 62-63 0.03142351451928249 1107 64-65 0.06978927265338797 1107 66-67 0.06432710241561068 1107 68-69 0.0916456882193799 1107 70-71 0.08785421486358302 1107 72-73 1.4680928349299407 1107 74-75 1.9361550233371965 1107 76-77 0.4886951846098917 1107 78-79 0.30754633038148427 1107 80-81 0.20657999153905138 1107 82-83 0.19744881612749765 1107 84-85 0.148062880655921 1107 86-87 0.21723306429187872 1107 88-89 0.2209856310482401 1107 90-91 0.1827287246038516 1107 92-93 0.12857864476964664 1107 94-95 0.13985027470587497 1107 96-97 0.13529302694682954 1107 98-99 0.14235811260233788 1107 100 0.15767672084096773 1108 1 0.28450817511284043 1108 2 0.14739486893375187 1108 3 0.07500786811313986 1108 4 0.04147447748708544 1108 5 0.023267355108878007 1108 6 0.03207739202272819 1108 7 0.05684881453571222 1108 8 0.0134761731682147 1108 9 -0.023020042082308123 1108 10-11 0.0597657453300684 1108 12-13 0.050302116520072104 1108 14-15 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-1.1104368233905788 2316 66-67 -0.9708362539533724 2316 68-69 -0.9588250903477729 2316 70-71 -2.1772168708639086 2316 72-73 -0.463048094003355 2316 74-75 0.8348803171388717 2316 76-77 -0.3421255608117413 2316 78-79 -1.7828237960136804 2316 80-81 -1.6153733747243066 2316 82-83 -0.8628187464283741 2316 84-85 -0.7496833426287246 2316 86-87 -0.7042019315801475 2316 88-89 -0.842285471072568 2316 90-91 -0.9413563592323406 2316 92-93 -0.8757191078547137 2316 94-95 -0.8678583551476251 2316 96-97 -0.8621475063450035 2316 98-99 -0.8954013901510507 2316 100 -0.9198293964760857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 1.0 6 24.0 7 683.0 8 1346.0 9 2034.0 10 7729.0 11 10880.0 12 8003.0 13 7957.0 14 12718.0 15 24851.0 16 42748.0 17 63140.0 18 85872.0 19 115741.0 20 166665.0 21 180835.0 22 218876.0 23 339779.0 24 545671.0 25 716841.0 26 733691.0 27 649921.0 28 622590.0 29 719348.0 30 808523.0 31 813669.0 32 959830.0 33 1224002.0 34 1719253.0 35 2803955.0 36 4702256.0 37 9118025.0 38 1.1634644E7 39 3385172.0 40 7485.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.255436495095452 36.762302907654224 26.680167476125167 19.302093121125154 2 21.35921536561922 33.214650834303995 27.61200760461809 17.814126195458694 3 24.774400438829485 28.4229737354062 26.65297664273633 20.149649183027986 4 18.872480404371935 27.36017596463559 28.030548840114772 25.7367947908777 5 19.78319365086065 33.11329402400468 26.307200688626175 20.796311636508495 6 27.362534315646453 26.755014673384437 26.62539434036123 19.25705667060788 7 21.050473988086942 27.02336150347715 32.673879976565175 19.25228453187074 8 25.411059334949943 28.308294012456887 26.787654123769727 19.492992528823446 9 25.245245179698227 28.01901666665921 27.279933446330478 19.455804707312083 10-11 19.859645181356463 31.058827821870334 29.80132215566222 19.280204841110983 12-13 19.88196844876015 27.289043039942527 29.4802171724012 23.348771338896118 14-15 19.11910251897456 34.29663065152496 26.335059532580768 20.249207296919714 16-17 22.497758215434665 27.853532234882255 26.291720518215744 23.356989031467332 18-19 20.20324976994923 30.36504458781641 27.018656495023325 22.413049147211034 20-21 22.508967722558502 30.36656031895034 27.03962469790489 20.084847260586265 22-23 23.029412321007406 28.118455459846093 29.508493971194138 19.343638247952367 24-25 19.5879988252733 30.948126582396263 26.34883364443647 23.11504094789397 26-27 19.281109518992288 27.17334498329359 33.416546998573665 20.128998499140454 28-29 19.340320750747605 28.0002682945054 32.61227535269657 20.04713560205043 30-31 20.086272256754647 27.24082724425272 33.32533466171713 19.3475658372755 32-33 19.374588924195134 28.055197899016942 32.46409719398381 20.106115982804116 34-35 19.39576140811123 28.100462782982895 32.425162165970356 20.078613642935515 36-37 19.388476201108467 28.107216610362446 32.39730029758157 20.10700689094752 38-39 19.46637163829763 28.8686207718078 32.34511585336302 19.319891736531545 40-41 19.48557643329309 28.170994273845167 32.323558652357654 20.019870640504088 42-43 19.502093691933446 27.467625414089703 33.001026303634546 20.0292545903423 44-45 19.552054804091977 27.498943415435335 32.92977688424031 20.019224896232373 46-47 20.316681844019218 27.63727649573346 32.81344289946144 19.232598760785883 48-49 19.611329698457624 28.424313074662354 32.69979227507397 19.264564951806054 50-51 20.40608599734558 27.74036913958502 31.79789056759848 20.05565429547092 52-53 20.491955617328017 27.903532195562097 31.529706505308475 20.07480568180141 54-55 20.003064723098753 28.141549451481072 31.602157665412328 20.253228160007847 56-57 20.351653594724755 28.631218525612955 31.33093803716454 19.686189842497754 58-59 20.567506946324436 29.04522198352299 29.94676761592716 20.440503454225414 60-61 21.30910414887075 29.292426700350504 28.876456291452122 20.52201285932662 62-63 20.585438699847845 29.423282727123645 30.184236845908806 19.807041727119703 64-65 21.35417223761078 30.282484374300427 28.521704366714633 19.841639021374156 66-67 20.542144991983513 30.9927862021556 28.534561442685728 19.93050736317516 68-69 20.507555434218762 30.879179144761405 28.620394154840618 19.992871266179215 70-71 20.439787602961403 30.76602134976763 28.687110834334263 20.107080212936708 72-73 21.027832235000076 30.414569356687473 28.436319383313435 20.12127902499902 74-75 20.421747398085568 30.51720530975376 28.559913991389557 20.50113330077112 76-77 20.428166960387205 30.55032174186537 28.363429984338477 20.65808131340894 78-79 20.52243189653681 30.630790116140773 27.99529032304339 20.851487664279034 80-81 20.523859608522095 30.75057206655873 27.673949894444448 21.051618430474726 82-83 20.51619014552458 30.886772120468503 27.351802694812267 21.24523503919465 84-85 20.468806615397472 30.90918274418895 27.145768371128348 21.47624226928523 86-87 20.4465819261352 30.96516980805336 26.93291677415922 21.655331491652216 88-89 20.419669056744553 30.97995560100511 26.813044558566943 21.787330783683394 90-91 20.395072803372464 30.998649456721417 26.643992411932725 21.962285327973387 92-93 20.365186775349084 30.986957087908824 26.55678576471314 22.091070372028952 94-95 20.3271942537619 31.00066093994012 26.487821705447452 22.184323100850538 96-97 20.283301236193456 30.94480956354358 26.41256838489306 22.359320815369898 98-99 20.225499171691787 30.892562810114217 26.391429366541715 22.49050865165228 100 20.14701590593492 30.890169594729556 26.33851472339634 22.624299775939186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 283560.0 1 171635.0 2 44322.0 3 26630.0 4 23770.0 5 23357.0 6 23427.5 7 23426.0 8 23815.5 9 24698.5 10 26868.5 11 38934.0 12 308100.0 13 358677.0 14 131908.5 15 118289.5 16 129626.0 17 142562.5 18 154959.5 19 168711.0 20 186563.0 21 212772.0 22 249150.0 23 292929.0 24 341029.0 25 393608.0 26 453057.5 27 523096.5 28 608489.5 29 697652.0 30 774093.0 31 844863.5 32 920862.0 33 983798.0 34 1037303.0 35 1101198.5 36 1166845.5 37 1232749.0 38 1302490.5 39 1352731.0 40 1385401.5 41 1428084.5 42 1477807.0 43 1491987.0 44 1481699.5 45 1480252.0 46 1469812.0 47 1451409.5 48 1419468.5 49 1374821.5 50 1330668.5 51 1276764.0 52 1209200.5 53 1118616.5 54 1011074.5 55 908640.0 56 811263.5 57 714336.0 58 615452.5 59 515125.0 60 417000.0 61 330949.0 62 255436.5 63 187121.5 64 133307.5 65 95488.0 66 69690.5 67 51802.0 68 38236.5 69 28610.5 70 21838.0 71 16982.5 72 13516.0 73 10831.5 74 8949.5 75 7700.5 76 5708.0 77 3291.5 78 1864.0 79 1192.5 80 801.0 81 582.5 82 408.0 83 268.5 84 178.5 85 109.0 86 69.5 87 45.0 88 31.0 89 26.5 90 19.5 91 13.0 92 7.5 93 4.0 94 5.0 95 4.5 96 2.0 97 0.5 98 1.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20475678592357574 2 7.066345612749798E-5 3 0.0 4 0.001823117168089448 5 8.832932015937247E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.1199036838249395E-5 14-15 5.4175316364415116E-5 16-17 0.0020209748452464423 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005491728265375384 26-27 0.03159245351033555 28-29 0.05599961125677336 30-31 0.08785587500331823 32-33 0.09274931934014748 34-35 0.10047165737061421 36-37 0.10767815217135022 38-39 0.09919853743605045 40-41 0.05965526738710259 42-43 0.04239807367649879 44-45 0.04635404949536988 46-47 0.08807493171731348 48-49 0.1169268208541709 50-51 0.13203466777422998 52-53 0.492377073675062 54-55 0.4781112996071889 56-57 0.48386919355731123 58-59 0.4766897864147574 60-61 0.4950387305225311 62-63 0.4837278666450562 64-65 0.46357818213030016 66-67 0.4695515996216113 68-69 0.4786660077377897 70-71 0.48645665377584646 72-73 1.1742723589598048 74-75 3.16022521762519 76-77 0.4851823561170139 78-79 0.4952283441298065 80-81 0.5196119473908685 82-83 0.530913389474193 84-85 0.5297768855548091 86-87 0.5027975780053304 88-89 0.4811074301469948 90-91 0.4543130253077164 92-93 0.4416725107307758 94-95 0.44563673061952846 96-97 0.46548138454866744 98-99 0.45890143905892855 100 0.4599013269631327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.2454759E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.17376760401451 #Duplication Level Percentage of deduplicated Percentage of total 1 62.29868700799928 20.666821648386062 2 17.504346957745895 11.613702760726015 3 7.383213260477627 7.3478700262188905 4 3.764934058160857 4.9958818995947 5 2.1150645549435696 3.508233000659317 6 1.351572286491629 2.690204695925989 7 0.9410538274496325 2.1852810682277735 8 0.6883253293682551 1.8267475609935389 9 0.5318452768552666 1.5878980454120433 >10 3.0506833991466387 18.093990060078234 >50 0.1866514406596364 4.239028752255081 >100 0.18015806521623995 12.138502981393037 >500 0.002633492547187577 0.5351868497796166 >1k 6.608548752826809E-4 0.5515770753839786 >5k 9.218520833076705E-5 0.20262232251942905 >10k+ 7.800285484321812E-5 7.816451252446241 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2240310 5.276934913233166 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG 336138 0.7917557605261638 TruSeq Adapter, Index 5 (97% over 36bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 297559 0.7008849113947391 No Hit AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACAAACATCTCGTATG 223254 0.5258633078096144 TruSeq Adapter, Index 5 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 108052 0.25451092538294706 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 48344 0.11387180410092541 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4745508271522634 0.0 0.0 2.355448537583266E-6 7.066345612749798E-5 2 1.47459322522594 2.355448537583266E-6 0.0 2.355448537583266E-6 7.066345612749798E-5 3 1.4746332678510787 2.355448537583266E-6 0.0 2.355448537583266E-6 7.066345612749798E-5 4 1.4746780213732928 1.177724268791633E-5 0.0 2.355448537583266E-6 7.066345612749798E-5 5 1.474694509513056 1.648813976308286E-5 0.0 4.710897075166532E-6 7.066345612749798E-5 6 1.474710997652819 2.1199036838249395E-5 0.0 4.710897075166532E-6 7.772980174024777E-5 7 1.4747204194469694 2.5909933913415927E-5 0.0 4.710897075166532E-6 7.772980174024777E-5 8 1.474746329380883 2.5909933913415927E-5 0.0 4.710897075166532E-6 7.772980174024777E-5 9 1.4747698838662586 2.5909933913415927E-5 0.0 4.710897075166532E-6 7.772980174024777E-5 10-11 1.474812281939935 2.708765818220756E-5 0.0 4.710897075166532E-6 8.008525027783104E-5 12-13 1.474904144432901 3.415400379495736E-5 0.0 8.244069881541432E-6 8.597387162178922E-5 14-15 1.4751385115623905 3.768717660133226E-5 0.0 1.177724268791633E-5 9.186249296574737E-5 16-17 1.475258639437807 3.768717660133226E-5 0.0 1.177724268791633E-5 9.539566577212226E-5 18-19 1.4753693455190735 3.768717660133226E-5 0.0 1.177724268791633E-5 9.65733900409139E-5 20-21 1.475474162978996 4.004262513891552E-5 0.0 1.884358830066613E-5 9.892883857849717E-5 22-23 1.4756802647260345 4.593124648287369E-5 0.0 1.884358830066613E-5 1.0717290846003861E-4 24-25 1.4763174135554509 4.9464419289248584E-5 0.0 1.884358830066613E-5 1.1659470261037166E-4 26-27 1.4783796087501049 5.299759209562349E-5 0.0 1.884358830066613E-5 1.2012787541674656E-4 28-29 1.4846521682056892 6.241938624595655E-5 0.0 1.884358830066613E-5 1.2012787541674656E-4 30-31 1.500439562028841 6.830800758991471E-5 0.0 2.1199036838249395E-5 1.2601649676070474E-4 32-33 1.5337526706958813 7.890752600903941E-5 0.0 2.1199036838249395E-5 1.319051181046629E-4 34-35 1.5927966991874811 8.715159589058084E-5 0.0 2.1199036838249395E-5 1.3426056664224615E-4 36-37 1.6835862853443593 8.715159589058084E-5 0.0 2.355448537583266E-5 1.4368236079257923E-4 38-39 1.812234995845813 8.715159589058084E-5 0.0 2.5909933913415927E-5 1.4368236079257923E-4 40-41 1.9834254623845586 8.715159589058084E-5 0.0 3.062083098858246E-5 1.4368236079257923E-4 42-43 2.198875984668762 9.304021723453901E-5 0.0 3.062083098858246E-5 1.460378093301625E-4 44-45 2.4620278730118335 9.539566577212226E-5 0.0 3.062083098858246E-5 1.460378093301625E-4 46-47 2.773639581842874 1.001065628472888E-4 0.0 3.062083098858246E-5 1.4721553359895413E-4 48-49 3.1342257766673463 1.0128428711608044E-4 0.0 3.297627952616572E-5 1.5192643067412064E-4 50-51 3.54434116561585 1.036397356536637E-4 0.0 3.297627952616572E-5 1.6017050055566208E-4 52-53 3.9975977722544602 1.0599518419124698E-4 0.0 3.297627952616572E-5 1.6252594909324535E-4 54-55 4.496260360352063 1.0835063272883023E-4 0.0 3.297627952616572E-5 1.63703673362037E-4 56-57 5.038298533269262 1.0835063272883023E-4 0.0 3.297627952616572E-5 1.672368461684119E-4 58-59 5.625773308476441 1.142392540727884E-4 0.0 3.297627952616572E-5 1.7312546751237006E-4 60-61 6.243275341640733 1.213055996855382E-4 0.0 3.533172806374899E-5 1.7783636458753658E-4 62-63 6.865511826365567 1.3072739383587127E-4 0.0 3.6509452332540624E-5 1.8372498593149475E-4 64-65 7.485863952260335 1.3426056664224615E-4 0.0 3.768717660133226E-5 1.8372498593149475E-4 66-67 8.137179626905901 1.3426056664224615E-4 0.0 3.768717660133226E-5 1.9550222861941107E-4 68-69 8.812020343820583 1.3426056664224615E-4 0.0 3.768717660133226E-5 2.037462985009525E-4 70-71 9.514465739871472 1.3426056664224615E-4 0.0 3.768717660133226E-5 2.0492402276974414E-4 72-73 10.244645364728132 1.4368236079257923E-4 0.0 3.8864900870123887E-5 2.072794713073274E-4 74-75 11.030033170132942 1.460378093301625E-4 0.0 4.004262513891552E-5 2.1199036838249395E-4 76-77 11.86809822663226 1.4721553359895413E-4 0.0 4.239807367649879E-5 2.1670126545766046E-4 78-79 12.72388214475555 1.4839325786774577E-4 0.0 4.239807367649879E-5 2.1670126545766046E-4 80-81 13.595789108118598 1.495709821365374E-4 0.0 4.239807367649879E-5 2.1670126545766046E-4 82-83 14.483454022198078 1.5310415494291228E-4 0.0 4.239807367649879E-5 2.2023443826403537E-4 84-85 15.386655474831455 1.5545960348049555E-4 0.0 4.475352221408205E-5 2.2376761107041027E-4 86-87 16.301329139567134 1.5545960348049555E-4 0.0 4.710897075166532E-5 2.2965623241436844E-4 88 16.993557777586254 1.5545960348049555E-4 0.0 4.710897075166532E-5 2.3083395668316008E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 291025 0.0 84.76157 8 AGTGGTA 294555 0.0 84.42262 5 TGGTATC 294300 0.0 83.721504 7 GTGGTAT 296695 0.0 83.506096 6 GTATCAA 295075 0.0 82.85401 9 GCAGTGG 304995 0.0 82.563255 3 AAGCAGT 309155 0.0 81.480484 1 CAGTGGT 313105 0.0 79.98097 4 AGCAGTG 321505 0.0 78.88621 2 CAACGCA 285805 0.0 44.59631 12-13 ATCAACG 286055 0.0 44.4954 10-11 ACGCAGA 292655 0.0 43.972214 14-15 AGAGTAC 297735 0.0 43.179447 18-19 TCAACGC 285905 0.0 42.57733 12-13 AACGCAG 288640 0.0 41.993736 14-15 AGTACTT 306390 0.0 41.82402 20-21 TATCAAC 293815 0.0 41.504868 10-11 CGCAGAG 293605 0.0 41.280296 16-17 GAGTACT 291620 0.0 41.111465 20-21 GCAGAGT 320060 0.0 40.322014 16-17 >>END_MODULE