FastQCFastQC Report
Thu 26 May 2016
SRR1660691_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660691_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46140374
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42910039.299887772907953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4974381.0780970262616425No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3692680.8003142757360397Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1135830.24616835572247422No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT779520.16894531457417314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5572550.088.07781
AGTGGTA5469700.088.060335
GGTATCA5454650.087.747458
GTGGTAT5502600.087.237326
TGGTATC5480000.087.182267
GCAGTGG5620800.086.9061053
GTATCAA5524850.085.820649
AGCAGTG5738250.085.810462
CAGTGGT5666200.085.404734
CAACGCA5437150.045.4461712-13
ATCAACG5446400.045.39675510-11
ACGCAGA5518500.045.1047714-15
AGAGTAC5519950.044.8099918-19
AGTACTT5572600.044.03691520-21
TCAACGC5434100.043.4922812-13
TATCAAC5472650.043.1907610-11
GCAGAGT5752550.043.0529416-17
AACGCAG5470150.042.98605314-15
GAGTACT5420250.042.42772720-21
CGCAGAG5531350.042.40284316-17
Produced by FastQC (version 0.11.5)