##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660691_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46140374 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23929673825357 34.0 31.0 34.0 30.0 34.0 2 32.38163732699696 34.0 31.0 34.0 30.0 34.0 3 32.35809161408184 34.0 31.0 34.0 30.0 34.0 4 35.76048065843593 37.0 35.0 37.0 35.0 37.0 5 35.78355138603774 37.0 35.0 37.0 35.0 37.0 6 35.800329121736205 37.0 36.0 37.0 35.0 37.0 7 35.68398676178914 37.0 35.0 37.0 35.0 37.0 8 35.77541267870954 37.0 35.0 37.0 35.0 37.0 9 37.471306127687654 39.0 38.0 39.0 35.0 39.0 10-11 37.32048582874512 39.0 37.0 39.0 34.5 39.0 12-13 37.447081150664275 39.0 38.0 39.0 35.0 39.0 14-15 38.88224382403142 41.0 39.0 41.0 35.0 41.0 16-17 38.805753373390516 40.5 39.0 41.0 35.0 41.0 18-19 38.616544558134706 40.0 38.0 41.0 34.0 41.0 20-21 38.48744284127389 40.0 38.0 41.0 34.0 41.0 22-23 38.255708612158195 40.0 37.5 41.0 33.5 41.0 24-25 38.3013233052684 40.0 38.0 41.0 34.0 41.0 26-27 38.35561377330838 40.0 38.0 41.0 34.0 41.0 28-29 38.28476986337388 40.0 38.0 41.0 34.0 41.0 30-31 38.1432407331592 40.0 38.0 41.0 33.5 41.0 32-33 38.0146790639365 40.0 38.0 41.0 33.0 41.0 34-35 37.91289224270267 40.0 38.0 41.0 33.0 41.0 36-37 37.67740401280666 39.5 37.5 41.0 33.0 41.0 38-39 37.13689064375595 39.5 35.0 41.0 32.5 41.0 40-41 37.15487112219766 40.0 35.0 41.0 32.0 41.0 42-43 36.973362450421405 39.5 35.0 41.0 31.5 41.0 44-45 36.83986040078479 39.0 35.0 41.0 31.0 41.0 46-47 36.8778582830733 39.0 35.0 41.0 31.0 41.0 48-49 36.82885943880733 39.0 35.0 41.0 31.0 41.0 50-51 35.3997918287355 38.0 34.0 40.0 28.5 40.5 52-53 35.406348494270986 38.5 34.5 39.5 27.5 40.5 54-55 35.566795839149464 39.0 35.0 41.0 24.5 41.0 56-57 34.98932413291665 39.0 35.0 41.0 20.5 41.0 58-59 34.759354193791324 39.0 35.0 41.0 20.0 41.0 60-61 34.60568546756903 38.5 34.0 41.0 20.0 41.0 62-63 34.39570087793393 38.0 34.0 41.0 18.5 41.0 64-65 34.09086236492145 37.0 34.0 40.0 18.5 41.0 66-67 33.70971804433141 37.0 33.5 40.0 17.5 41.0 68-69 33.435864574916536 36.0 33.0 40.0 17.0 41.0 70-71 33.14041233172492 36.0 33.0 39.0 17.0 41.0 72-73 32.836515401890765 35.0 33.0 39.0 17.0 41.0 74-75 32.45651109156592 35.0 33.0 38.5 15.0 40.0 76-77 32.059134186905375 35.0 32.0 37.0 13.5 39.5 78-79 31.66414884283339 35.0 32.0 37.0 10.5 39.0 80-81 31.26063927223477 35.0 31.0 36.0 10.0 39.0 82-83 30.958308259486582 35.0 31.0 36.0 10.0 37.5 84-85 30.659382377351342 35.0 31.0 35.0 10.0 37.0 86-87 30.3936647544296 35.0 31.0 35.0 10.0 37.0 88-89 30.208955805169676 35.0 30.0 35.0 10.0 36.0 90-91 30.0013911460709 35.0 30.0 35.0 10.0 36.0 92-93 29.83125276574481 35.0 30.0 35.0 10.0 36.0 94-95 29.669484495292558 35.0 29.5 35.0 9.0 35.5 96-97 29.516389203954006 34.0 29.0 35.0 8.0 35.0 98-99 29.372782999114833 34.0 29.0 35.0 8.0 35.0 100 29.251285956199663 34.0 29.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30753516229432876 1101 2 0.29434413548251115 1101 3 0.2929368815549793 1101 4 0.25138420770260694 1101 5 0.2719548994568939 1101 6 0.1785360150101667 1101 7 0.22658371445030667 1101 8 0.22230997011916287 1101 9 0.3164371219662172 1101 10-11 0.2859494312501525 1101 12-13 0.2960562854199722 1101 14-15 0.3411649255917055 1101 16-17 0.33157392455859025 1101 18-19 0.38830180212263343 1101 20-21 0.3141166737674652 1101 22-23 0.32200575786107066 1101 24-25 0.3263026387143313 1101 26-27 0.3266576557015526 1101 28-29 0.33346273806775883 1101 30-31 0.3300573122267778 1101 32-33 0.2998044383554941 1101 34-35 0.29925961371302634 1101 36-37 0.27756147429303013 1101 38-39 0.3220411109828305 1101 40-41 0.2973678407838136 1101 42-43 0.29108422032869896 1101 44-45 0.29442432811020325 1101 46-47 0.30891800901071775 1101 48-49 0.33822458167268366 1101 50-51 0.26135829081282225 1101 52-53 0.3340232823264415 1101 54-55 0.4156078664615279 1101 56-57 0.4055108255243809 1101 58-59 0.3651026543919542 1101 60-61 0.3667325074624941 1101 62-63 0.3184536705660932 1101 64-65 0.3127562978956391 1101 66-67 0.2920212500251935 1101 68-69 0.27070053258666604 1101 70-71 0.2763614999342252 1101 72-73 0.25468733821183775 1101 74-75 0.2455466568423148 1101 76-77 0.22412793506070727 1101 78-79 0.2026541494778975 1101 80-81 0.21990467422487114 1101 82-83 0.19650804036657732 1101 84-85 0.15301029664484744 1101 86-87 0.17154155324467268 1101 88-89 0.1495428796075693 1101 90-91 0.1263207827407271 1101 92-93 0.1256346722083812 1101 94-95 0.09843929531725948 1101 96-97 0.07503221776237368 1101 98-99 0.05746936125329327 1101 100 0.04101151226017308 1102 1 0.31938152282748433 1102 2 0.29695478290384614 1102 3 0.29562069330827967 1102 4 0.27176611574334686 1102 5 0.2561907444269451 1102 6 0.20272543876315297 1102 7 0.19645827245422254 1102 8 0.19996252014576754 1102 9 0.3131747033393282 1102 10-11 0.3081480189400665 1102 12-13 0.27898843589704114 1102 14-15 0.3192033688517171 1102 16-17 0.3440380109866723 1102 18-19 0.35984076535708454 1102 20-21 0.3318512680904817 1102 22-23 0.2964154511982926 1102 24-25 0.30167564786128054 1102 26-27 0.3320310453768016 1102 28-29 0.30413846508309916 1102 30-31 0.3274202171177052 1102 32-33 0.3292437030453854 1102 34-35 0.3339191588270367 1102 36-37 0.2974213628661957 1102 38-39 0.30971108797607627 1102 40-41 0.2577384666134108 1102 42-43 0.24448122172037756 1102 44-45 0.22901064741234478 1102 46-47 0.2700200726875366 1102 48-49 0.29195535751067325 1102 50-51 0.2560088443277948 1102 52-53 0.29087491929221443 1102 54-55 0.3914123222214414 1102 56-57 0.39588556112435214 1102 58-59 0.3705448093496173 1102 60-61 0.29604766847693753 1102 62-63 0.27597317191352033 1102 64-65 0.2306177883764704 1102 66-67 0.27649482325681873 1102 68-69 0.25826990417427 1102 70-71 0.284997982897508 1102 72-73 0.22146511579906303 1102 74-75 0.2220252155771334 1102 76-77 0.1878524939852042 1102 78-79 0.19314385575049542 1102 80-81 0.22127205867167277 1102 82-83 0.15570997026634004 1102 84-85 0.1500807593876452 1102 86-87 0.2058712650983523 1102 88-89 0.15216302891121458 1102 90-91 0.1836079288473833 1102 92-93 0.13831717249806275 1102 94-95 0.09935798641768301 1102 96-97 0.09427597730045534 1102 98-99 0.10358130200166826 1102 100 0.08915967095458655 1103 1 0.25023930412156403 1103 2 0.23614018388194324 1103 3 0.2468671899875261 1103 4 0.21635563254211831 1103 5 0.21206155717257502 1103 6 0.13548022494000378 1103 7 0.15599238123288472 1103 8 0.16426958683270243 1103 9 0.2236864350075507 1103 10-11 0.21805106019223786 1103 12-13 0.22793549979537175 1103 14-15 0.2410663227801777 1103 16-17 0.26121486637782 1103 18-19 0.2771966834458439 1103 20-21 0.2507815145400514 1103 22-23 0.26986546410774537 1103 24-25 0.25002158146728704 1103 26-27 0.25976440056447103 1103 28-29 0.24896489377386644 1103 30-31 0.2055795969486809 1103 32-33 0.20120927352969176 1103 34-35 0.21880650493168474 1103 36-37 0.21179332033393905 1103 38-39 0.20384139282329272 1103 40-41 0.20574894476636985 1103 42-43 0.16147911164925688 1103 44-45 0.17333202260041247 1103 46-47 0.23579993595153326 1103 48-49 0.2283554174432183 1103 50-51 0.20766631058430818 1103 52-53 0.2754497357038588 1103 54-55 0.38031774418408304 1103 56-57 0.36706649892096976 1103 58-59 0.3264575102786509 1103 60-61 0.3281950893375196 1103 62-63 0.30039132588737516 1103 64-65 0.3029072583394026 1103 66-67 0.3018732975990659 1103 68-69 0.2563486536461923 1103 70-71 0.26514356124364724 1103 72-73 0.21247178070439077 1103 74-75 0.25845227656503056 1103 76-77 0.24907356307308248 1103 78-79 0.22230820215056113 1103 80-81 0.24519316495405263 1103 82-83 0.22503815139084438 1103 84-85 0.2238103514820864 1103 86-87 0.22299601646155054 1103 88-89 0.14678685858622842 1103 90-91 0.16385770758102325 1103 92-93 0.1608616160459171 1103 94-95 0.12718467832173275 1103 96-97 0.09014208158618331 1103 98-99 0.09771753268286787 1103 100 0.08142747688561158 1104 1 0.18635771972632398 1104 2 0.16706832869349597 1104 3 0.1684434283002787 1104 4 0.18947505696136346 1104 5 0.14797724493938347 1104 6 0.12314749500097832 1104 7 0.1243004105163692 1104 8 0.12752128320949652 1104 9 0.16741028626864107 1104 10-11 0.18862318425616564 1104 12-13 0.1901796723511211 1104 14-15 0.18797297675592972 1104 16-17 0.2084690096077182 1104 18-19 0.24953420091531342 1104 20-21 0.2200013294066423 1104 22-23 0.21100898858600914 1104 24-25 0.22490373808291508 1104 26-27 0.23687885662506147 1104 28-29 0.19742330130011254 1104 30-31 0.21061003368147624 1104 32-33 0.1858527656630855 1104 34-35 0.20357139780139022 1104 36-37 0.1719382026428491 1104 38-39 0.21158550975023616 1104 40-41 0.20982481553988208 1104 42-43 0.17625148969018767 1104 44-45 0.14956354656449378 1104 46-47 0.14361582946661144 1104 48-49 0.18431817992384225 1104 50-51 0.16181949293453357 1104 52-53 0.18256196605659625 1104 54-55 0.21795917079134597 1104 56-57 0.2657060593044136 1104 58-59 0.2187800282191361 1104 60-61 0.1841218939655178 1104 62-63 0.1743779354790007 1104 64-65 0.19548781276685645 1104 66-67 0.2220512038039928 1104 68-69 0.2168927774656524 1104 70-71 0.24254592101721784 1104 72-73 0.1635559067709238 1104 74-75 0.1802260110757814 1104 76-77 0.1653112660481071 1104 78-79 0.15397078255677243 1104 80-81 0.19726766660890505 1104 82-83 0.1647372744593376 1104 84-85 0.16608525153343834 1104 86-87 0.16847826861713955 1104 88-89 0.13502444813468983 1104 90-91 0.14109908925775017 1104 92-93 0.11163182166503915 1104 94-95 0.08555905986844792 1104 96-97 0.07025701359971848 1104 98-99 0.06908517100049849 1104 100 0.034624558635435676 1105 1 0.1380261934219007 1105 2 0.12658346998420456 1105 3 0.1670273255759014 1105 4 0.16610538901085903 1105 5 0.13663067936830942 1105 6 0.07397605875922864 1105 7 0.09135742908952693 1105 8 0.09866604263904577 1105 9 0.1362059198879635 1105 10-11 0.10615278360689473 1105 12-13 0.1240851296102008 1105 14-15 0.13921618249023737 1105 16-17 0.1326798687722217 1105 18-19 0.20099119573828972 1105 20-21 0.168362000003917 1105 22-23 0.1444650022411409 1105 24-25 0.1397625845791879 1105 26-27 0.1465054398667931 1105 28-29 0.12536857125306256 1105 30-31 0.09997834441838904 1105 32-33 0.13164966130170797 1105 34-35 0.13361118004065275 1105 36-37 0.07920540173814317 1105 38-39 0.11879271596117036 1105 40-41 0.11589769690070284 1105 42-43 0.12767688421880052 1105 44-45 0.1095205228502607 1105 46-47 0.1491543780512785 1105 48-49 0.13095957381241163 1105 50-51 0.08395718867259916 1105 52-53 0.13256437446282376 1105 54-55 0.19128824495656715 1105 56-57 0.20117207853479613 1105 58-59 0.1799038390829324 1105 60-61 0.1664406270052936 1105 62-63 0.15122539359592935 1105 64-65 0.1572216483547706 1105 66-67 0.18429301006234056 1105 68-69 0.20319088170882083 1105 70-71 0.18899490334564462 1105 72-73 0.17650147712629405 1105 74-75 0.15523491653002708 1105 76-77 0.13859678853920343 1105 78-79 0.17169342316942604 1105 80-81 0.18289076038433905 1105 82-83 0.18818092241417972 1105 84-85 0.1542736734186505 1105 86-87 0.11629488187001513 1105 88-89 0.06884261357563659 1105 90-91 0.11539343131710211 1105 92-93 0.14158294347625855 1105 94-95 0.07452583264842261 1105 96-97 0.09895415182716505 1105 98-99 0.09530198262902445 1105 100 0.08131483980712417 1106 1 0.09167886270227399 1106 2 0.08068884872047732 1106 3 0.09913235466089532 1106 4 0.10059928449033606 1106 5 0.09898780162169629 1106 6 0.011617375364735949 1106 7 0.05385932581457098 1106 8 0.07995612686028153 1106 9 0.11924703313517426 1106 10-11 0.08158886023547751 1106 12-13 0.05827949336981675 1106 14-15 0.06828900547873218 1106 16-17 0.06630471655309833 1106 18-19 0.1377119170856389 1106 20-21 0.11888718775222884 1106 22-23 0.1075149095671506 1106 24-25 0.08030466181556051 1106 26-27 0.1280412817552019 1106 28-29 0.05557592179372506 1106 30-31 0.0925637608250156 1106 32-33 0.10111848096208575 1106 34-35 0.016346014345266724 1106 36-37 0.024834902482332666 1106 38-39 0.03316119291223174 1106 40-41 0.04678884491999469 1106 42-43 0.06349421995180649 1106 44-45 0.04935643783384336 1106 46-47 0.03274610769518205 1106 48-49 0.03615755333797921 1106 50-51 -0.07614145651541548 1106 52-53 0.013987072459535455 1106 54-55 0.091467147324785 1106 56-57 0.17275361279502732 1106 58-59 0.1352027534301996 1106 60-61 0.07201629140489985 1106 62-63 0.05654477266006808 1106 64-65 0.02176483183274769 1106 66-67 0.04613545086936455 1106 68-69 0.06366606877116254 1106 70-71 0.12720532308014754 1106 72-73 0.08918185668035505 1106 74-75 0.015459890692454792 1106 76-77 -0.013646151597747291 1106 78-79 0.06589436143380212 1106 80-81 0.12436345459406795 1106 82-83 0.1275604654604905 1106 84-85 0.11750829530832974 1106 86-87 0.10997792569868281 1106 88-89 0.05715573310190791 1106 90-91 0.062020316871368664 1106 92-93 0.039177572727105314 1106 94-95 0.04405171867590241 1106 96-97 0.0447435067915265 1106 98-99 0.04538170126746266 1106 100 0.02811036848851245 1107 1 0.04191107114441195 1107 2 0.04338361004914759 1107 3 0.04726990827336408 1107 4 0.07726173472774178 1107 5 0.06582040795617417 1107 6 0.016040944684760916 1107 7 -0.018786873374729396 1107 8 -0.02246413147736348 1107 9 0.01088885853749133 1107 10-11 0.0017420496958067133 1107 12-13 0.04078392212367987 1107 14-15 -0.003212894127578636 1107 16-17 -0.006775586914564258 1107 18-19 0.05899084883865413 1107 20-21 0.03006510848484112 1107 22-23 0.038311743382045904 1107 24-25 -0.007056266213950835 1107 26-27 -0.015779402548830035 1107 28-29 0.0172380349807284 1107 30-31 -0.016023338860406966 1107 32-33 -0.05470458062833217 1107 34-35 -0.018426344003607653 1107 36-37 -0.04319800484075387 1107 38-39 0.026044172862761172 1107 40-41 -0.014149895014348601 1107 42-43 -0.07552132184416394 1107 44-45 -0.03015576573830714 1107 46-47 -0.008304952238887608 1107 48-49 -0.026424028419974377 1107 50-51 -0.08875383756051747 1107 52-53 0.006891472882493588 1107 54-55 0.0398695057463172 1107 56-57 0.11269968906821504 1107 58-59 0.12538004299563 1107 60-61 0.08899454046797928 1107 62-63 0.08894109190926969 1107 64-65 0.10952986869454406 1107 66-67 0.14602653919591546 1107 68-69 0.11812986101014644 1107 70-71 0.14600663963535965 1107 72-73 0.11338264068359649 1107 74-75 0.10364214443325892 1107 76-77 0.06069472388143993 1107 78-79 0.05737465806938857 1107 80-81 0.11349964980973581 1107 82-83 0.0490438410787597 1107 84-85 0.09465238629207917 1107 86-87 0.15431325548674835 1107 88-89 0.07834650641420637 1107 90-91 0.09205749278164532 1107 92-93 0.08714826610555093 1107 94-95 0.0727706093277547 1107 96-97 0.06680256032969112 1107 98-99 0.07641704290740492 1107 100 0.07871440894480841 1108 1 -0.02505458213770595 1108 2 -0.035864015565209684 1108 3 0.013505282662741536 1108 4 0.026630237139926294 1108 5 0.042343640601508525 1108 6 0.00905999666167645 1108 7 -0.01024738264446512 1108 8 0.017833596435956167 1108 9 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#Quality Count 2 117.0 3 245.0 4 142.0 5 101.0 6 418.0 7 4705.0 8 13708.0 9 22543.0 10 28722.0 11 32563.0 12 40646.0 13 52982.0 14 71703.0 15 100913.0 16 136873.0 17 176870.0 18 192879.0 19 213133.0 20 241547.0 21 298838.0 22 408032.0 23 625674.0 24 935372.0 25 1162778.0 26 1163808.0 27 986680.0 28 713738.0 29 660691.0 30 720537.0 31 856547.0 32 1052011.0 33 1345288.0 34 1854426.0 35 3146030.0 36 5083693.0 37 8706670.0 38 1.1403384E7 39 3677814.0 40 7553.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.469850070985494 40.68188163235573 25.618162662730036 17.230105633928737 2 19.77475758573307 38.12319256560199 24.90290869812318 17.19914115054176 3 29.435598089404237 26.37781838146881 25.373896579752447 18.812686949374505 4 18.19426286531729 26.27462802440756 25.521247744100574 30.00986136617458 5 18.788448810802436 37.09311751492171 24.854004095541598 19.26442957873426 6 31.03699882156982 25.796849896515734 24.982472523589475 18.18367875832497 7 19.702940789013244 25.736322512087533 36.208769889653524 18.3519668092457 8 30.00231722721316 26.968013441261913 24.692998828249152 18.33667050327578 9 29.494655360829793 26.752768119516702 25.325260274542664 18.427316245110845 10-11 18.605251248243267 32.511962241020406 30.622012690942856 18.260773819793467 12-13 18.56950940871483 26.5658779112237 30.31938304470178 24.54522963535969 14-15 18.586148717164164 37.671068859647306 25.1967439708811 18.546038452307428 16-17 24.439139973082757 26.295819392200677 24.713931779689293 24.551108855027273 18-19 19.05829111136764 32.15404165754109 25.127265533605918 23.660401697485344 20-21 24.034626087687897 32.6483621561169 25.150284700744745 18.166727055450455 22-23 24.538892315714154 26.83812815114396 30.401857089022343 18.221122444119544 24-25 18.613923897932374 32.35566427472876 24.989314783286982 24.041097044051877 26-27 18.186943954431573 27.432287858494952 36.185843922364015 18.19492426470946 28-29 18.665059760076623 27.03590858788006 36.159959927693755 18.139071724349563 30-31 18.672801276642314 26.573879921310745 36.56150287381293 18.19181592823402 32-33 18.64691405730526 26.642100227659544 36.504479610577754 18.206506104457443 34-35 18.666547544859675 27.173615352043957 35.98952421675244 18.170312886343925 36-37 18.209572883005304 27.159190893346242 36.426127348353624 18.20510887529483 38-39 18.258521537670326 26.71163732733476 36.37553893976789 18.654302195227025 40-41 18.76881803231942 26.779128234713827 35.862686633164216 18.589367099802537 42-43 18.788481856204267 26.840704312722806 36.2594181096021 18.111395721470824 44-45 19.28889214670389 26.92233451160131 35.69247845194873 18.09629488974607 46-47 18.394722160816737 27.032945878593516 36.029219419590234 18.543112540999516 48-49 18.930796854026916 27.060008593072503 35.42730921268054 18.581885340220044 50-51 19.02554270423828 27.12838322373456 35.26708642278332 18.578987649243835 52-53 18.71831456348505 27.771608830236854 35.363318753044965 18.14675785323313 54-55 19.202433129742445 27.731845111178867 34.23957185835428 18.82614990072441 56-57 19.883067552303977 28.954175596030822 32.567084735218714 18.595672116446483 58-59 20.176129783504752 29.63320678504811 31.625694997098158 18.564968434348977 60-61 20.36717500147436 30.464924891765694 30.64248563712542 18.525414469634523 62-63 20.54222397696618 30.650586522349478 30.345440936580037 18.4617485641043 64-65 20.69555520955089 30.677994262615528 30.27093953455769 18.355510993275896 66-67 20.85526422231884 30.35149136402653 30.517416958717597 18.275827454937033 68-69 21.020379939270004 29.897892347961037 30.871264765260293 18.210462947508667 70-71 21.25690477469469 29.58275633589487 31.08662206476061 18.07371682464983 72-73 21.485277284463823 29.395087601183395 31.108288159540027 18.011346954812755 74-75 21.790596420270962 29.408199792733242 30.863770181989263 17.937433605006532 76-77 22.16323183325811 29.529730886205325 30.410278068552195 17.896759211984374 78-79 22.56951805602213 29.696617873630316 29.86796693290537 17.865897137442186 80-81 22.9386260428692 29.882890046053813 29.359233254043215 17.81925065703377 82-83 23.17358364900665 30.100827972695864 28.933740552775966 17.791847825521522 84-85 23.39835515922657 30.141193809280548 28.640588013919544 17.819863017573343 86-87 23.638795989956535 30.114696800505214 28.447885748946756 17.798621460591495 88-89 23.885237298674433 30.095040104566916 28.27153119212426 17.74819140463439 90-91 24.152405172707038 30.02588074402227 28.057500521330812 17.764213561939872 92-93 24.327743184105433 29.972725108128373 27.939238916860564 17.76029279090563 94-95 24.512838549797415 29.934902021207233 27.83094237757816 17.72131705141719 96-97 24.672844818715852 29.87900880191441 27.698611468171038 17.749534911198705 98-99 24.81940807300737 29.866143899834974 27.562694148750584 17.751753878407076 100 24.91604872489284 29.918237881696484 27.424506604113507 17.741206789297166 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 404830.0 1 238822.5 2 58776.5 3 42890.0 4 40457.0 5 40222.0 6 41045.0 7 40965.5 8 40625.0 9 40589.5 10 41972.0 11 63781.0 12 614631.0 13 699284.0 14 225988.0 15 205327.0 16 222495.5 17 241286.0 18 256155.5 19 270329.5 20 288542.0 21 314579.0 22 351878.0 23 394120.0 24 434626.5 25 479328.0 26 533773.5 27 604953.5 28 691307.5 29 775585.0 30 849409.5 31 922022.0 32 996217.5 33 1060335.5 34 1114570.0 35 1172128.0 36 1235127.0 37 1298134.5 38 1361150.0 39 1395643.0 40 1396129.5 41 1405674.5 42 1436780.0 43 1439555.0 44 1425895.0 45 1435602.0 46 1442219.0 47 1432010.0 48 1399181.0 49 1359518.0 50 1324210.5 51 1281263.0 52 1224673.5 53 1142596.5 54 1045102.5 55 956351.0 56 874234.5 57 788129.0 58 697800.0 59 598085.5 60 490218.0 61 395175.5 62 311703.5 63 232977.0 64 170640.0 65 127177.5 66 94943.0 67 71159.5 68 53024.5 69 39789.0 70 31133.0 71 25227.5 72 21097.0 73 16878.0 74 13359.5 75 11014.5 76 8347.0 77 5347.5 78 3492.5 79 2531.0 80 1805.5 81 1373.0 82 1099.5 83 841.5 84 658.5 85 529.5 86 410.5 87 341.5 88 291.0 89 258.5 90 215.5 91 175.5 92 145.5 93 124.5 94 108.5 95 83.0 96 56.5 97 46.0 98 40.0 99 23.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004492811436682329 2 8.88592710583577E-5 3 0.09029835778964428 4 0.02574101371610035 5 0.017897557570729705 6 0.05555871740441463 7 0.03825283254097593 8 0.025936070652569913 9 0.005535282397147453 10-11 0.007659235705371611 12-13 0.014712711258040516 14-15 0.027158427454445862 16-17 0.050057028146325816 18-19 0.06308358055355165 20-21 0.04585680211434784 22-23 0.02705439708832876 24-25 0.01924778503095792 26-27 0.01751719654461405 28-29 0.012504233277346212 30-31 0.010786648586766982 32-33 0.008059102425134222 34-35 0.005908057875733734 36-37 0.010188473981593647 38-39 0.014607597242276364 40-41 0.016100866455915595 42-43 0.009946820110300797 44-45 0.00378627186680368 46-47 0.002740549957397398 48-49 0.0019115579774017437 50-51 0.0010543911065827077 52-53 0.0014802654178745927 54-55 2.817489082338171E-4 56-57 0.002536823823751407 58-59 0.010878758806766498 60-61 0.022205064917765947 62-63 0.027568047021031952 64-65 0.009090736889128814 66-67 0.002207394331047252 68-69 0.0015972995797563323 70-71 0.0022008924331649326 72-73 7.14125117408021E-4 74-75 6.881175258787456E-4 76-77 0.0014184973879925638 78-79 0.0032845420802180753 80-81 0.0075335323463134474 82-83 0.008820908127012582 84-85 0.0076007186244307435 86-87 0.004596841802799431 88-89 0.002416538712928508 90-91 0.0026972039715152723 92-93 0.0026787819275153686 94-95 0.003332222664688414 96-97 0.0034774317173935348 98-99 0.0076993307423125785 100 0.0133787385425181 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.6140374E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.806915580292753 #Duplication Level Percentage of deduplicated Percentage of total 1 65.77654082750341 20.26372340436577 2 15.684140694435673 9.663599966458278 3 6.426427415448756 5.939352206118262 4 3.269500169204768 4.028928628097766 5 1.958126165916953 3.0161913744482947 6 1.267598827665192 2.3430486044135774 7 0.9182950524989644 1.9802886712095271 8 0.6572711422018603 1.6198797272940244 9 0.5437963798511923 1.5077420250250042 >10 3.0564123081478862 17.065994855091425 >50 0.21825506668441502 4.576539837968978 >100 0.21686831726408398 13.660206443739757 >500 0.005468726955276022 1.095750652711081 >1k 8.799283691936348E-4 0.5517468073046555 >5k 2.513867325105426E-4 0.521205758112551 >10k+ 1.6759111983312024E-4 12.165801037640962 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4291003 9.299887772907953 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 497438 1.0780970262616425 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 369268 0.8003142757360397 Illumina Single End PCR Primer 1 (100% over 50bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113583 0.24616835572247422 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 77952 0.16894531457417314 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.9272659991876095 2.1672992941062854E-6 0.0 0.0 7.36881759996137E-5 2 0.9273461892614915 2.1672992941062854E-6 0.0 2.1672992941062854E-6 7.585547529371999E-5 3 0.9274437177297262 4.334598588212571E-6 2.1672992941062854E-6 6.501897882318856E-6 7.585547529371999E-5 4 0.9274957329127848 6.501897882318856E-6 2.1672992941062854E-6 6.501897882318856E-6 7.802277458782627E-5 5 0.9275304097014905 8.669197176425142E-6 2.1672992941062854E-6 6.501897882318856E-6 8.019007388193256E-5 6 0.9275780902859608 1.5171095058743997E-5 2.1672992941062854E-6 6.501897882318856E-6 8.019007388193256E-5 7 0.9276105997753724 1.9505693646956568E-5 2.1672992941062854E-6 6.501897882318856E-6 8.235737317603884E-5 8 0.9276799533527839 1.9505693646956568E-5 2.1672992941062854E-6 6.501897882318856E-6 8.235737317603884E-5 9 0.927744972331607 2.1672992941062853E-5 2.1672992941062854E-6 6.501897882318856E-6 8.235737317603884E-5 10-11 0.9278099913104303 2.817489082338171E-5 2.1672992941062854E-6 6.501897882318856E-6 8.235737317603884E-5 12-13 0.9279226908737237 3.467678870570057E-5 2.1672992941062854E-6 8.669197176425142E-6 8.235737317603884E-5 14-15 0.9280527288313701 4.009503694096628E-5 2.1672992941062854E-6 1.3003795764637713E-5 8.452467247014513E-5 16-17 0.928153508248546 5.09315334114977E-5 2.1672992941062854E-6 1.3003795764637713E-5 8.452467247014513E-5 18-19 0.9283171393452511 5.743343129381656E-5 2.1672992941062854E-6 1.5171095058743999E-5 8.777562141130455E-5 20-21 0.9284894396391326 6.610262847024171E-5 2.1672992941062854E-6 1.7338394352850284E-5 8.88592710583577E-5 22-23 0.9287072532181901 7.152087670550742E-5 2.1672992941062854E-6 1.7338394352850284E-5 8.88592710583577E-5 24-25 0.9293455228603045 8.235737317603884E-5 2.1672992941062854E-6 2.2756642588115995E-5 9.102657035246398E-5 26-27 0.9314933164607638 8.560832211719827E-5 8.669197176425142E-6 2.817489082338171E-5 9.536116894067655E-5 28-29 0.9383658225223749 8.88592710583577E-5 1.0836496470531426E-5 2.817489082338171E-5 9.752846823478283E-5 30-31 0.9548817267931118 9.102657035246398E-5 1.0836496470531426E-5 2.9258540470434852E-5 9.752846823478283E-5 32-33 0.986163441154595 9.536116894067655E-5 1.0836496470531426E-5 3.142583976454114E-5 9.752846823478283E-5 34-35 1.0393803916717277 1.018630668229954E-4 1.0836496470531426E-5 3.250948941159428E-5 1.018630668229954E-4 36-37 1.1240654442896367 1.0619766541120799E-4 1.0836496470531426E-5 3.250948941159428E-5 1.0619766541120799E-4 38-39 1.2480035380727517 1.0944861435236741E-4 1.0836496470531426E-5 3.467678870570057E-5 1.0836496470531427E-4 40-41 1.4126478471977708 1.1269956329352684E-4 1.0836496470531426E-5 3.684408799980685E-5 1.1053226399942056E-4 42-43 1.6157833917861177 1.1269956329352684E-4 1.0836496470531426E-5 4.009503694096628E-5 1.1269956329352684E-4 44-45 1.8603934593161295 1.1703416188173941E-4 1.0836496470531426E-5 4.117868658801942E-5 1.1269956329352684E-4 46-47 2.1553141290098776 1.2787065835227085E-4 1.0836496470531426E-5 4.551328517623199E-5 1.1269956329352684E-4 48-49 2.5072044279485035 1.3220525694048341E-4 1.0836496470531426E-5 4.7680584470338275E-5 1.1486686258763312E-4 50-51 2.909332724524513 1.3220525694048341E-4 1.0836496470531426E-5 4.7680584470338275E-5 1.1595051223468627E-4 52-53 3.350975221830668 1.343725562345897E-4 1.0836496470531426E-5 5.09315334114977E-5 1.1811781152879255E-4 54-55 3.83098433489074 1.3653985552869598E-4 1.0836496470531426E-5 5.4182482352657135E-5 1.192014611758457E-4 56-57 4.354558331061643 1.495436512933337E-4 1.0836496470531426E-5 5.8517080940869705E-5 1.2136876046995196E-4 58-59 4.926664660325466 1.5279460023449312E-4 1.0836496470531426E-5 6.068438023497599E-5 1.2787065835227085E-4 60-61 5.539908497490723 1.6363109670502454E-4 1.0836496470531426E-5 6.285167952908228E-5 1.2787065835227085E-4 62-63 6.179822036119603 1.777185421167154E-4 1.0836496470531426E-5 6.393532917613542E-5 1.2787065835227085E-4 64-65 6.84405158917871 1.853040896460874E-4 1.0836496470531426E-5 6.935357741140113E-5 1.2787065835227085E-4 66-67 7.535654132322378 1.8638773929314053E-4 1.0836496470531426E-5 7.36881759996137E-5 1.2787065835227085E-4 68-69 8.258156945151766 1.8747138894019367E-4 1.5171095058743997E-5 7.585547529371999E-5 1.2787065835227085E-4 70-71 9.00841852734007 1.8855503858724681E-4 1.9505693646956568E-5 8.452467247014513E-5 1.2787065835227085E-4 72-73 9.775450671466166 1.907223378813531E-4 1.9505693646956568E-5 8.669197176425141E-5 1.2787065835227085E-4 74-75 10.553678000095967 1.9180598752840624E-4 1.9505693646956568E-5 9.211021999951712E-5 1.2787065835227085E-4 76-77 11.348753913438152 1.9288963717545938E-4 1.9505693646956568E-5 9.536116894067655E-5 1.2787065835227085E-4 78-79 12.16283379064071 2.0047518470483138E-4 1.9505693646956568E-5 9.752846823478283E-5 1.2787065835227085E-4 80-81 12.995562194619403 2.080607322342034E-4 2.1672992941062853E-5 9.861211788183598E-5 1.28954307999324E-4 82-83 13.837730270673575 2.1998087835178797E-4 2.1672992941062853E-5 1.0403036611710169E-4 1.3003795764637713E-4 84-85 14.687283419072415 2.2648277623410682E-4 2.1672992941062853E-5 1.116159136464737E-4 1.3220525694048341E-4 86-87 15.543272362725105 2.4490482023401027E-4 2.1672992941062853E-5 1.1486686258763312E-4 1.3220525694048341E-4 88 16.189842761135832 2.57908615998648E-4 2.1672992941062853E-5 1.1486686258763312E-4 1.3220525694048341E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 557255 0.0 88.0778 1 AGTGGTA 546970 0.0 88.06033 5 GGTATCA 545465 0.0 87.74745 8 GTGGTAT 550260 0.0 87.23732 6 TGGTATC 548000 0.0 87.18226 7 GCAGTGG 562080 0.0 86.906105 3 GTATCAA 552485 0.0 85.82064 9 AGCAGTG 573825 0.0 85.81046 2 CAGTGGT 566620 0.0 85.40473 4 CAACGCA 543715 0.0 45.44617 12-13 ATCAACG 544640 0.0 45.396755 10-11 ACGCAGA 551850 0.0 45.10477 14-15 AGAGTAC 551995 0.0 44.80999 18-19 AGTACTT 557260 0.0 44.036915 20-21 TCAACGC 543410 0.0 43.49228 12-13 TATCAAC 547265 0.0 43.19076 10-11 GCAGAGT 575255 0.0 43.05294 16-17 AACGCAG 547015 0.0 42.986053 14-15 GAGTACT 542025 0.0 42.427727 20-21 CGCAGAG 553135 0.0 42.402843 16-17 >>END_MODULE