FastQCFastQC Report
Thu 26 May 2016
SRR1660691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46140374
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33610967.284500988223459No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4332450.9389715826750775No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGAAATCTCGTATG2281920.49456036052070146TruSeq Adapter, Index 13 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTGAAATCTCGTATG1522190.32990413124956464TruSeq Adapter, Index 4 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1404130.30431699578334587No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT854150.18511986920608836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4411800.085.5507661
AGTGGTA4382150.085.072895
GGTATCA4363900.084.764838
GTGGTAT4420600.084.0256656
TGGTATC4401000.083.9824757
GCAGTGG4494450.083.9331053
GTATCAA4432350.082.700539
AGCAGTG4603450.082.490652
CAGTGGT4575300.081.989314
CAACGCA4339650.044.44563712-13
ATCAACG4337500.044.3817210-11
ACGCAGA4408700.044.11380414-15
AGAGTAC4446200.043.60439318-19
AGTACTT4500800.042.792520-21
TCAACGC4336200.042.09824412-13
TATCAAC4395600.041.59144610-11
GCAGAGT4685300.041.58139416-17
AACGCAG4368550.041.5607214-15
GAGTACT4327350.041.32663320-21
CGCAGAG4429100.040.96231516-17
Produced by FastQC (version 0.11.5)