##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660691_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46140374 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30089734426513 34.0 31.0 34.0 31.0 34.0 2 32.660321023839124 34.0 31.0 34.0 31.0 34.0 3 32.81554224072826 34.0 31.0 34.0 31.0 34.0 4 36.251474684622195 37.0 37.0 37.0 35.0 37.0 5 36.175146651390385 37.0 37.0 37.0 35.0 37.0 6 36.18104792561933 37.0 37.0 37.0 35.0 37.0 7 36.064503356648125 37.0 35.0 37.0 35.0 37.0 8 36.12020362036944 37.0 36.0 37.0 35.0 37.0 9 37.886966867672115 39.0 38.0 39.0 35.0 39.0 10-11 37.7532244103613 39.0 37.5 39.0 35.0 39.0 12-13 37.85116447040503 39.0 38.0 39.0 35.0 39.0 14-15 39.34777793522003 41.0 39.0 41.0 36.0 41.0 16-17 39.27980354038743 41.0 39.0 41.0 36.0 41.0 18-19 39.18375732064938 40.5 39.0 41.0 35.5 41.0 20-21 39.11733375849966 40.5 39.0 41.0 35.0 41.0 22-23 38.91820811855578 40.0 38.5 41.0 34.5 41.0 24-25 38.9263700918419 40.0 39.0 41.0 35.0 41.0 26-27 38.914789724071156 40.0 38.0 41.0 35.0 41.0 28-29 38.821366478303794 40.0 38.0 41.0 35.0 41.0 30-31 38.691780716818634 40.0 38.0 41.0 35.0 41.0 32-33 38.54219972295847 40.0 38.0 41.0 34.5 41.0 34-35 38.43441564864645 40.0 38.0 41.0 34.0 41.0 36-37 38.21101904158817 40.0 38.0 41.0 34.0 41.0 38-39 37.667094235083574 40.0 35.5 41.0 33.5 41.0 40-41 37.54156846452956 40.0 35.0 41.0 33.0 41.0 42-43 37.46938616058898 40.0 35.0 41.0 33.0 41.0 44-45 37.52609392589666 40.0 35.0 41.0 33.0 41.0 46-47 37.395952035412634 40.0 35.0 41.0 33.0 41.0 48-49 37.26357601696076 40.0 35.0 41.0 33.0 41.0 50-51 37.06589041735986 39.5 35.0 41.0 32.5 41.0 52-53 36.594618045358715 39.0 35.0 41.0 30.5 41.0 54-55 35.996090484658836 39.0 35.0 41.0 28.0 41.0 56-57 35.514902317436785 39.0 35.0 41.0 24.5 41.0 58-59 35.27246894444332 39.0 35.0 41.0 23.0 41.0 60-61 35.119005526483164 38.5 35.0 41.0 24.0 41.0 62-63 34.9347787146242 38.0 35.0 40.5 24.0 41.0 64-65 34.64264253688104 37.5 34.5 40.0 23.0 41.0 66-67 34.380031087307614 37.0 34.0 40.0 23.0 41.0 68-69 34.10705439882217 36.5 34.0 39.5 22.0 41.0 70-71 33.70284468218658 36.0 34.0 39.0 21.5 41.0 72-73 32.005751091224354 35.0 32.0 39.0 11.0 40.5 74-75 31.12814772589403 35.0 30.0 37.5 9.0 40.0 76-77 30.745055209132026 33.5 29.5 36.0 15.0 39.0 78-79 32.03283316472467 35.0 32.5 37.0 17.5 39.0 80-81 32.03701647498566 35.0 33.0 36.0 17.0 39.0 82-83 31.788738394274827 35.0 33.0 36.0 16.0 37.5 84-85 31.545385587901823 35.0 33.0 36.0 15.0 37.0 86-87 31.2555437240279 35.0 32.5 35.0 13.0 37.0 88-89 31.06492425700754 35.0 32.0 35.0 11.0 36.0 90-91 30.939112110361307 35.0 32.0 35.0 10.0 36.0 92-93 30.802075845765792 35.0 32.0 35.0 10.0 36.0 94-95 30.670122461512776 35.0 32.0 35.0 10.0 35.5 96-97 30.531334108821916 35.0 32.0 35.0 10.0 35.0 98-99 30.423740366733917 35.0 32.0 35.0 10.0 35.0 100 30.35320780017951 35.0 32.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5411939059598936 1101 2 0.38513290155171376 1101 3 0.30136428161146966 1101 4 0.2156366435265653 1101 5 0.20759642611953666 1101 6 0.19620532858775164 1101 7 0.24384453926867167 1101 8 0.24414637481872603 1101 9 0.29273045780107765 1101 10-11 0.2957511505455628 1101 12-13 0.2915971375077717 1101 14-15 0.3157734464217299 1101 16-17 0.3634777984026627 1101 18-19 0.37817745849042694 1101 20-21 0.340800572612288 1101 22-23 0.3336340725205389 1101 24-25 0.36097166211477116 1101 26-27 0.33211973310953624 1101 28-29 0.3269142489492012 1101 30-31 0.3039830907805694 1101 32-33 0.3821809669494698 1101 34-35 0.33391214706146854 1101 36-37 0.32942102605417034 1101 38-39 0.3696641396542546 1101 40-41 0.38014311228550923 1101 42-43 0.3430926161825383 1101 44-45 0.3464093444579319 1101 46-47 0.28316653487210885 1101 48-49 0.22930558501108322 1101 50-51 0.18257057875092642 1101 52-53 0.13285293990345082 1101 54-55 0.12333144548376396 1101 56-57 0.20262062376880863 1101 58-59 0.2318024740035014 1101 60-61 0.1305049771236284 1101 62-63 0.17278005968576338 1101 64-65 0.14492180469274984 1101 66-67 0.1367346381160388 1101 68-69 0.16475403712967562 1101 70-71 0.20512867180012506 1101 72-73 1.4356539131808006 1101 74-75 1.9271747383481284 1101 76-77 0.5793195444476851 1101 78-79 0.3348745657951113 1101 80-81 0.27102011210779153 1101 82-83 0.23321522347524137 1101 84-85 0.176943684717628 1101 86-87 0.21790398844875725 1101 88-89 0.2172681494907387 1101 90-91 0.14198223971735757 1101 92-93 0.10866432171428642 1101 94-95 0.09960882901141588 1101 96-97 0.08963263479969896 1101 98-99 0.10222667546996078 1101 100 0.10534879841936373 1102 1 0.5217417133386704 1102 2 0.3703147185245257 1102 3 0.29838006929713146 1102 4 0.20875660769477378 1102 5 0.1789475471879456 1102 6 0.19199197102831533 1102 7 0.23199680029115655 1102 8 0.2329616158055856 1102 9 0.26809826865961384 1102 10-11 0.27262834827043037 1102 12-13 0.2660469659027811 1102 14-15 0.32622207408168435 1102 16-17 0.34586791770055214 1102 18-19 0.37414643255537783 1102 20-21 0.3223438675919752 1102 22-23 0.32050769535026546 1102 24-25 0.33792721437054496 1102 26-27 0.3153233449055648 1102 28-29 0.34348208072385233 1102 30-31 0.3188142023862568 1102 32-33 0.35100905109009517 1102 34-35 0.3502704407324373 1102 36-37 0.346616734380909 1102 38-39 0.3780606810791127 1102 40-41 0.37783317444267794 1102 42-43 0.33992736655775246 1102 44-45 0.36505167998533494 1102 46-47 0.3018603404117215 1102 48-49 0.2518770483718171 1102 50-51 0.237666651954477 1102 52-53 0.12444935093912335 1102 54-55 0.14616455117424465 1102 56-57 0.1854410814233418 1102 58-59 0.25132079474246183 1102 60-61 0.20887766563665622 1102 62-63 0.21696213302980993 1102 64-65 0.1506609157184542 1102 66-67 0.1813121971777747 1102 68-69 0.19896557164791062 1102 70-71 0.20874444137185577 1102 72-73 1.4786789313311246 1102 74-75 1.9438600630897156 1102 76-77 0.6010186450580406 1102 78-79 0.3431873926114051 1102 80-81 0.22733223371712796 1102 82-83 0.19746695809052994 1102 84-85 0.179500043758118 1102 86-87 0.22220483192404217 1102 88-89 0.19340383798794036 1102 90-91 0.1233780961589126 1102 92-93 0.0968744951966869 1102 94-95 0.11619414534967376 1102 96-97 0.12829838252503478 1102 98-99 0.12188149455835884 1102 100 0.10524026048708279 1103 1 0.4600799436672247 1103 2 0.32122700724629993 1103 3 0.24042893546215538 1103 4 0.16582532291909047 1103 5 0.1758553476924476 1103 6 0.15237733863475 1103 7 0.17926439713554743 1103 8 0.1935458459224364 1103 9 0.19414777629562963 1103 10-11 0.21244552124652927 1103 12-13 0.2171455670289788 1103 14-15 0.23216151917461048 1103 16-17 0.28472784625748915 1103 18-19 0.29160782797386275 1103 20-21 0.25294662146895064 1103 22-23 0.25015036473888586 1103 24-25 0.2601650457775051 1103 26-27 0.21439607504115088 1103 28-29 0.26596449395803745 1103 30-31 0.20754735125404267 1103 32-33 0.2653572414207943 1103 34-35 0.2693983525015753 1103 36-37 0.23506707167907592 1103 38-39 0.2846378405677825 1103 40-41 0.27911367450146685 1103 42-43 0.24385525894607696 1103 44-45 0.254127105502711 1103 46-47 0.21133713571052226 1103 48-49 0.18157084759466358 1103 50-51 0.19655999707420335 1103 52-53 0.08978618641304337 1103 54-55 0.06849710298396872 1103 56-57 0.11694141587454254 1103 58-59 0.19042400824655914 1103 60-61 0.11859403069542651 1103 62-63 0.12460133269984652 1103 64-65 0.0642945257433567 1103 66-67 0.10880064355755792 1103 68-69 0.1611303495059886 1103 70-71 0.12894201356635193 1103 72-73 1.3959825447865342 1103 74-75 1.836288742883319 1103 76-77 0.43013091810376025 1103 78-79 0.252298332152769 1103 80-81 0.15681011222365981 1103 82-83 0.13233083167812154 1103 84-85 0.10827513810760081 1103 86-87 0.16149096441150945 1103 88-89 0.1089773870489914 1103 90-91 0.04784833698350255 1103 92-93 0.042984748351695856 1103 94-95 0.03691257316858909 1103 96-97 0.04639612797724979 1103 98-99 0.01606662939691006 1103 100 0.05667010926102023 1104 1 0.4210516696461042 1104 2 0.29567312332413564 1104 3 0.20709394973356865 1104 4 0.14621147260933043 1104 5 0.14772800321063073 1104 6 0.13429861511718855 1104 7 0.1698520396943053 1104 8 0.16723096901016277 1104 9 0.19321146652325183 1104 10-11 0.17996572408660683 1104 12-13 0.20597509935581826 1104 14-15 0.20626690655203106 1104 16-17 0.24667486429851948 1104 18-19 0.2567983070017448 1104 20-21 0.21414482313394245 1104 22-23 0.21212294046793545 1104 24-25 0.2433333697961544 1104 26-27 0.2285026430641608 1104 28-29 0.2484057136653206 1104 30-31 0.1954486699782194 1104 32-33 0.23426326613698478 1104 34-35 0.2473351966469295 1104 36-37 0.22709465118503402 1104 38-39 0.2598412451913461 1104 40-41 0.2778449083141439 1104 42-43 0.24347687268994633 1104 44-45 0.2315659584635128 1104 46-47 0.19509579913832908 1104 48-49 0.18616771971932877 1104 50-51 0.1593656813255535 1104 52-53 0.0952963658754058 1104 54-55 0.09648673955682341 1104 56-57 0.1146204915495943 1104 58-59 0.18291323866987597 1104 60-61 0.12977808591320183 1104 62-63 0.12815689541687192 1104 64-65 0.12300204342589183 1104 66-67 0.11625909959839476 1104 68-69 0.15710762387829647 1104 70-71 0.1475369128636217 1104 72-73 1.3919413015188056 1104 74-75 1.8946017635848804 1104 76-77 0.5267622372748413 1104 78-79 0.29918766023366317 1104 80-81 0.175468945546001 1104 82-83 0.1686690527927155 1104 84-85 0.18443230130714028 1104 86-87 0.23049664045707274 1104 88-89 0.22230690937502828 1104 90-91 0.11316406139738788 1104 92-93 0.08805326756311871 1104 94-95 0.10401997719386813 1104 96-97 0.11415412361538202 1104 98-99 0.13641860789011062 1104 100 0.12483209437567311 1105 1 0.3909572963879242 1105 2 0.25467461781546774 1105 3 0.1837527890477091 1105 4 0.13526415739551823 1105 5 0.12402103999764336 1105 6 0.12563647496165942 1105 7 0.1164683816879446 1105 8 0.13574988371165375 1105 9 0.12844652997839745 1105 10-11 0.15991855510731767 1105 12-13 0.1499683804012264 1105 14-15 0.15577487642557486 1105 16-17 0.1795915477525014 1105 18-19 0.22646096208407585 1105 20-21 0.19169084859608887 1105 22-23 0.16265894454723906 1105 24-25 0.2035558481216384 1105 26-27 0.1639224085993547 1105 28-29 0.1983652563216225 1105 30-31 0.1748436609314794 1105 32-33 0.21455549346123348 1105 34-35 0.20572248344449662 1105 36-37 0.16897084098646076 1105 38-39 0.19682564789456336 1105 40-41 0.20213494341189886 1105 42-43 0.1911833422838427 1105 44-45 0.1765701816197094 1105 46-47 0.1738182021255028 1105 48-49 0.14923654882206705 1105 50-51 0.13225433508165452 1105 52-53 0.06963452187402197 1105 54-55 0.02414023131722587 1105 56-57 0.06148062146314004 1105 58-59 0.15389945696667695 1105 60-61 0.08476618029975924 1105 62-63 0.06704144353756192 1105 64-65 0.09882331832369573 1105 66-67 0.09927925171855634 1105 68-69 0.10581686315715189 1105 70-71 0.07738152471525694 1105 72-73 1.3825221747245067 1105 74-75 1.8588545663453573 1105 76-77 0.4415579819299964 1105 78-79 0.26103531782871414 1105 80-81 0.19411191354142687 1105 82-83 0.15693861149433985 1105 84-85 0.14704696072446666 1105 86-87 0.23632718729279745 1105 88-89 0.1906806945669679 1105 90-91 0.1338566048840626 1105 92-93 0.09522537436372858 1105 94-95 0.1412172176641029 1105 96-97 0.11631469592364851 1105 98-99 0.09321407830406869 1105 100 0.09129753995735257 1106 1 0.34069748305924463 1106 2 0.217769776617736 1106 3 0.1375171239481432 1106 4 0.0946508871922731 1106 5 0.08998972332923927 1106 6 0.07528877505951925 1106 7 0.07423395989410153 1106 8 0.1028015313106323 1106 9 0.09316298414481139 1106 10-11 0.11731018006191363 1106 12-13 0.1195830982633268 1106 14-15 0.08102531140070823 1106 16-17 0.121825021439669 1106 18-19 0.1495363401521672 1106 20-21 0.0971613541271168 1106 22-23 0.07993622645886944 1106 24-25 0.13106016410121413 1106 26-27 0.12316883787769939 1106 28-29 0.12225681261086407 1106 30-31 0.12158490261808907 1106 32-33 0.16621657610806295 1106 34-35 0.1460310065424082 1106 36-37 0.12794239371159932 1106 38-39 0.1508849591897743 1106 40-41 0.17145694207401618 1106 42-43 0.1763636806253217 1106 44-45 0.16765061770912126 1106 46-47 0.12071251246993597 1106 48-49 0.07929112349373213 1106 50-51 0.08880478917997436 1106 52-53 0.026485951667858387 1106 54-55 -0.02615854614064972 1106 56-57 -0.0027425976775461436 1106 58-59 0.0582319414479997 1106 60-61 0.053646881780970546 1106 62-63 0.055581467675686724 1106 64-65 0.06670624810419667 1106 66-67 0.05405734863400369 1106 68-69 0.08049408327949692 1106 70-71 0.08395214160598385 1106 72-73 1.4035559570263914 1106 74-75 1.879050237222188 1106 76-77 0.42965264054509333 1106 78-79 0.27190238929998856 1106 80-81 0.1641461111981002 1106 82-83 0.13697771658151936 1106 84-85 0.15284064108606898 1106 86-87 0.2246360273095469 1106 88-89 0.1889538057687119 1106 90-91 0.10524032646094028 1106 92-93 0.08088986214127658 1106 94-95 0.10930171171898095 1106 96-97 0.158264369712402 1106 98-99 0.16238865900815824 1106 100 0.14502266771135552 1107 1 0.318823383680531 1107 2 0.19015059014348878 1107 3 0.10732265744959335 1107 4 0.08364702968111004 1107 5 0.08660512336900439 1107 6 0.07168559521294071 1107 7 0.07631020483299267 1107 8 0.06039577771746707 1107 9 0.08487210744252138 1107 10-11 0.08773612153106569 1107 12-13 0.08963862304049286 1107 14-15 0.0719869905530004 1107 16-17 0.10192902710250706 1107 18-19 0.1051789496473674 1107 20-21 0.06978554410589766 1107 22-23 0.10636312775555012 1107 24-25 0.0934315643059378 1107 26-27 0.06016468794497598 1107 28-29 0.07085284374267076 1107 30-31 0.06902922118890586 1107 32-33 0.08315425030892243 1107 34-35 0.05681658986001281 1107 36-37 0.07327171153962553 1107 38-39 0.09898579526867479 1107 40-41 0.0966833190132732 1107 42-43 0.06007184933977072 1107 44-45 0.09948383267227001 1107 46-47 0.06565038420815483 1107 48-49 0.06207077512168979 1107 50-51 0.04138695226065181 1107 52-53 0.01874404217938519 1107 54-55 -0.001105691081939142 1107 56-57 0.0011913491040687063 1107 58-59 0.07095934907309953 1107 60-61 -0.0171515902375603 1107 62-63 -0.018102848488432244 1107 64-65 0.016170440491102056 1107 66-67 0.015889752235608512 1107 68-69 0.04578678474211273 1107 70-71 0.07575948650384134 1107 72-73 1.3941747105741271 1107 74-75 1.8312267336223869 1107 76-77 0.4133337083866806 1107 78-79 0.21966160940841206 1107 80-81 0.1448119644525221 1107 82-83 0.16911821235932933 1107 84-85 0.12428121403080539 1107 86-87 0.17864791686049486 1107 88-89 0.14379104811991184 1107 90-91 0.071712522787589 1107 92-93 0.06553422007584686 1107 94-95 0.07964473724942778 1107 96-97 0.06669852391835462 1107 98-99 0.07665492073997981 1107 100 0.09631706392551109 1108 1 0.27902468507419087 1108 2 0.12522961021243617 1108 3 0.07250614947096778 1108 4 0.0476510623960138 1108 5 0.03298380151966285 1108 6 0.02211261103751383 1108 7 0.041546313726577466 1108 8 0.022514018538345226 1108 9 0.007483534483711196 1108 10-11 0.03524680539243974 1108 12-13 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-1.4273607220155284 2316 52-53 -1.2593498617713195 2316 54-55 -1.2986718490909652 2316 56-57 -1.0954819824394022 2316 58-59 -0.9247435765733059 2316 60-61 -0.9063055259477437 2316 62-63 -0.9675480970376853 2316 64-65 -1.0792151514840285 2316 66-67 -0.9801122419925647 2316 68-69 -0.9373130401675027 2316 70-71 -2.1469367454792163 2316 72-73 -0.44051281496516026 2316 74-75 0.7994020726679594 2316 76-77 -0.3142106349734206 2316 78-79 -1.7301344733867872 2316 80-81 -1.537132604871985 2316 82-83 -0.8395670471121086 2316 84-85 -0.7401239645711293 2316 86-87 -0.6723739422912942 2316 88-89 -0.8146231215836011 2316 90-91 -0.9192255257185629 2316 92-93 -0.8489453649537175 2316 94-95 -0.8621387797218958 2316 96-97 -0.8973534618803072 2316 98-99 -0.9025998571733886 2316 100 -0.9248351168257116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 36.0 7 789.0 8 1639.0 9 2300.0 10 8400.0 11 11768.0 12 8886.0 13 9193.0 14 15284.0 15 32789.0 16 60444.0 17 94650.0 18 131262.0 19 159196.0 20 217907.0 21 237660.0 22 294274.0 23 455350.0 24 734526.0 25 950690.0 26 942430.0 27 795813.0 28 715282.0 29 759773.0 30 850632.0 31 913862.0 32 1083256.0 33 1367936.0 34 1916282.0 35 3224028.0 36 5260320.0 37 9476041.0 38 1.1851722E7 39 3549057.0 40 6896.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.029194852754184 38.130746121134095 26.187692228707853 19.652366797403868 2 19.931083294861935 35.36178983694896 27.73424259991859 16.972884268270516 3 26.642341477336096 27.648152570241415 26.051741583195664 19.65776436922683 4 18.128583587966858 27.302122311133243 26.931511542644838 27.637782558255058 5 19.407604373266256 35.361201748349416 25.72588030821689 19.50531357016744 6 28.738609704377343 26.603822066981948 26.166380879357416 18.491187349283297 7 20.008992558231107 26.870347864973958 34.55169435774405 18.568965219050888 8 27.317192964235616 27.52495244186794 26.31321757383241 18.844637020064035 9 26.967778804740504 27.131685148455887 27.07841943370463 18.822116613098974 10-11 18.93414322996168 31.876432124282307 30.481419591440677 18.70800505431534 12-13 18.967914480515837 27.30666994651375 29.956539678248085 23.768875894722328 14-15 18.521210827399447 36.418508962329064 25.57151820478171 19.48876200548977 16-17 23.143553924760678 27.562015622071716 25.532964048584617 23.761466404582993 18-19 19.38618876387955 31.678812356397458 25.991180739020454 22.943818140702543 20-21 23.123292195247487 31.714280209345507 26.040163870366545 19.12226372504046 22-23 23.21443254881289 28.035058840225265 30.05878864354242 18.691719967419424 24-25 18.77115449465012 31.932122946950557 25.750922483811888 23.54580007458743 26-27 18.587785149070413 27.313726426255368 34.957913779507194 19.14057464516702 28-29 18.612221150493294 27.89020932253817 34.42191476516704 19.0756547618015 30-31 19.047634180409894 27.429553830759996 34.859322412865865 18.66348957596425 32-33 18.61892502450987 27.977579733921115 34.280592344642876 19.122902896926135 34-35 18.62926353264724 27.548801701283466 34.71284126978308 19.10909349628622 36-37 19.122870538213334 27.550834262784647 34.66490165838472 18.6613935406173 38-39 19.175027688079076 28.07360023041237 34.130038722199735 18.621333359308817 40-41 18.699323648470692 28.605203473087926 34.12695874068088 18.5685141377605 42-43 18.703908708674017 27.721974362431833 34.52076310070115 19.053353828192996 44-45 18.75661528561072 27.781962419621458 34.42667351242921 19.03474878233861 46-47 19.236696713325326 27.928797046989455 34.283425276787796 18.55108096289742 48-49 18.818249884746795 28.445154104057274 34.14426551873718 18.592330492458753 50-51 19.32375246419468 28.071913083566642 33.51386796394626 19.090466488292424 52-53 19.39108403544041 28.236941705681005 33.21817466084348 19.153799598035103 54-55 19.26402214905874 28.532758024470983 32.841064277551126 19.362155548919155 56-57 19.755123828844642 29.15116481734113 31.96943810219448 19.12427325161974 58-59 20.015991480791982 29.696061583381116 30.69241942422024 19.595527511606665 60-61 20.46702436544789 30.055785623978228 29.82126945945746 19.655920551116417 62-63 20.015852068097185 30.250985307006445 30.505703164193793 19.22745946070258 64-65 20.487674102834767 30.84530018150591 29.40180456412454 19.265221151534785 66-67 19.94914631212285 31.226382363962557 29.464214031572922 19.36025729234167 68-69 19.896126354830137 31.029658110486597 29.669076360582018 19.405139174101244 70-71 19.83536629434702 30.893454886997436 29.79658796287862 19.47459085577693 72-73 20.413980708770172 30.55219106627845 29.568403765118173 19.465424459833205 74-75 19.83929924766315 30.68692341833535 29.67465316252363 19.799124171477875 76-77 19.900172048142988 30.73350126170598 29.45920838253555 19.907118307615477 78-79 20.044856177854147 30.847521895140844 29.026985398099175 20.080636528905835 80-81 20.086804930340175 30.967178842378274 28.696518227800098 20.24949799948145 82-83 20.12341114709204 31.105928625724143 28.347744406322427 20.42291582086139 84-85 20.13649704990623 31.0974519209248 28.160480185635013 20.605570843533958 86-87 20.188737661335782 31.095413310800968 27.960026648233182 20.755822379630064 88-89 20.20926008037472 31.101041716221996 27.845668586465305 20.84402961693798 90-91 20.243076350580928 31.04129892401501 27.71497710354312 21.000647621860942 92-93 20.27605105533784 30.99401720634137 27.667854651318336 21.06207708700245 94-95 20.310258662705376 30.962864460247776 27.6263765373073 21.100500339739547 96-97 20.3230004787481 30.896448465141695 27.565506368272928 21.215044687837274 98-99 20.312214725526815 30.840193038790918 27.557857983698653 21.28973425198361 100 20.246586400137414 30.810273266309395 27.538368440713594 21.40477189283959 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 382596.0 1 238230.0 2 72604.5 3 48531.5 4 44693.5 5 43987.0 6 45066.5 7 45859.0 8 46483.0 9 47422.5 10 50261.5 11 69995.5 12 529702.5 13 609640.0 14 212865.0 15 183421.0 16 197234.0 17 214117.0 18 229640.5 19 245160.5 20 267083.5 21 300053.5 22 344193.5 23 393354.5 24 440967.0 25 492930.0 26 553067.5 27 628115.5 28 719435.0 29 810696.5 30 889368.5 31 964107.5 32 1042710.5 33 1110270.0 34 1161336.0 35 1214345.5 36 1274969.0 37 1335814.0 38 1396160.5 39 1429028.5 40 1430405.5 41 1439731.5 42 1466875.0 43 1468568.5 44 1453329.5 45 1450291.0 46 1439977.5 47 1416775.0 48 1378198.0 49 1342081.0 50 1312465.0 51 1269111.5 52 1207213.5 53 1120151.0 54 1021886.0 55 933743.5 56 850322.5 57 762852.5 58 671030.5 59 572288.5 60 467686.0 61 376057.0 62 295457.0 63 219055.5 64 160444.0 65 119357.0 66 88523.0 67 65732.0 68 48226.5 69 36143.5 70 28094.0 71 22374.5 72 18451.0 73 14618.0 74 11346.5 75 9178.0 76 6727.0 77 4059.5 78 2443.0 79 1648.5 80 1069.5 81 718.0 82 507.5 83 333.0 84 205.5 85 128.0 86 94.5 87 61.0 88 31.5 89 22.5 90 17.0 91 10.5 92 7.0 93 8.5 94 6.0 95 3.5 96 3.5 97 1.5 98 0.5 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20440233102575195 2 8.019007388193256E-5 3 0.0 4 0.001868211991519618 5 8.625851190543015E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.492394188222228E-5 14-15 5.2015183058550844E-5 16-17 0.001974409656930826 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005575377434088419 26-27 0.031616562102422495 28-29 0.056120047921588145 30-31 0.0878764008284805 32-33 0.09236162671763345 34-35 0.10020074826441588 36-37 0.1074135203152016 38-39 0.09888736489218747 40-41 0.059641909274510865 42-43 0.04235119550613092 44-45 0.04623607949081644 46-47 0.08793275061012726 48-49 0.11660828757044753 50-51 0.13147812802731074 52-53 0.4917862174242454 54-55 0.47730107259208604 56-57 0.48331424448358395 58-59 0.4760982214838571 60-61 0.4945495240242309 62-63 0.48267055659323443 64-65 0.4627053954959273 66-67 0.4687239856356605 68-69 0.4781961672005519 70-71 0.48577521283204167 72-73 1.1764414826806562 74-75 3.158184196773091 76-77 0.48455393967981275 78-79 0.4948020143919943 80-81 0.519122363420808 82-83 0.5304746771233367 84-85 0.5294506282068715 86-87 0.5025739496606595 88-89 0.48023559583630593 90-91 0.4535073772917402 92-93 0.44098688926968815 94-95 0.4446994729604923 96-97 0.4645454325966235 98-99 0.4583198653743032 100 0.45904916158677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.6140374E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.409217786323282 #Duplication Level Percentage of deduplicated Percentage of total 1 63.971565878579106 19.453252789338393 2 16.513728324514947 10.04339122168701 3 6.680678593593195 6.094626309390102 4 3.3978162640154097 4.132997390814237 5 2.0627021004457102 3.136257870038004 6 1.3831132997616964 2.5235636133368198 7 0.9542537177672459 2.0312676388894992 8 0.7384233456219875 1.796390106841959 9 0.5628863430356225 1.5405240054885587 >10 3.2655249330988125 18.016182848147885 >50 0.2343462545302512 4.878606984663166 >100 0.22754958254250093 14.222517182714187 >500 0.006120676602497524 1.222089411486318 >1k 9.853566533895948E-4 0.6369234117269604 >5k 1.98819061576231E-4 0.43411516548204415 >10k+ 1.0651017610945781E-4 9.837294049954668 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3361096 7.284500988223459 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 433245 0.9389715826750775 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGAAATCTCGTATG 228192 0.49456036052070146 TruSeq Adapter, Index 13 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTGAAATCTCGTATG 152219 0.32990413124956464 TruSeq Adapter, Index 4 (97% over 35bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140413 0.30431699578334587 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 85415 0.18511986920608836 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.9286530707358376 4.334598588212571E-6 2.1672992941062854E-6 6.501897882318856E-6 4.984788376444456E-5 2 0.9287375954083077 6.501897882318856E-6 2.1672992941062854E-6 8.669197176425142E-6 4.984788376444456E-5 3 0.9288091162850132 6.501897882318856E-6 2.1672992941062854E-6 8.669197176425142E-6 4.984788376444456E-5 4 0.9288611314680718 1.7338394352850284E-5 2.1672992941062854E-6 1.0836496470531426E-5 4.984788376444456E-5 5 0.9288958082567774 2.3840292235169137E-5 2.1672992941062854E-6 1.3003795764637713E-5 4.984788376444456E-5 6 0.9289413215419537 3.684408799980685E-5 2.1672992941062854E-6 1.3003795764637713E-5 4.984788376444456E-5 7 0.928969496432777 3.684408799980685E-5 2.1672992941062854E-6 1.3003795764637713E-5 4.984788376444456E-5 8 0.9290388500101885 4.3345985882125706E-5 2.1672992941062854E-6 1.3003795764637713E-5 4.984788376444456E-5 9 0.9290973670911293 5.201518305855085E-5 2.1672992941062854E-6 1.3003795764637713E-5 4.984788376444456E-5 10-11 0.9291840590628936 6.393532917613542E-5 2.1672992941062854E-6 1.5171095058743997E-5 4.984788376444456E-5 12-13 0.9292924240275989 6.826992776434799E-5 2.1672992941062854E-6 2.492394188222228E-5 4.984788376444456E-5 14-15 0.9294809790661862 7.260452635256056E-5 4.334598588212571E-6 2.817489082338171E-5 5.09315334114977E-5 16-17 0.9296045151259502 7.477182564666685E-5 4.334598588212571E-6 2.817489082338171E-5 5.201518305855085E-5 18-19 0.9297648952737141 7.585547529371999E-5 4.334598588212571E-6 2.817489082338171E-5 6.068438023497599E-5 20-21 0.9299296100200661 8.344102282309198E-5 4.334598588212571E-6 2.9258540470434852E-5 6.176802988202914E-5 22-23 0.9301040776132417 9.644481858772969E-5 4.334598588212571E-6 3.142583976454114E-5 6.285167952908228E-5 24-25 0.9307055031673562 1.018630668229954E-4 6.501897882318856E-6 3.7927737646859994E-5 6.718627811729485E-5 26-27 0.9328793043593449 1.018630668229954E-4 6.501897882318856E-6 4.442963552917885E-5 6.718627811729485E-5 28-29 0.9397604796181322 1.0294671647004855E-4 6.501897882318856E-6 4.7680584470338275E-5 6.718627811729485E-5 30-31 0.9562189504575754 1.0836496470531427E-4 6.501897882318856E-6 4.984788376444456E-5 7.36881759996137E-5 32-33 0.9877249802959984 1.1486686258763312E-4 6.501897882318856E-6 4.984788376444456E-5 7.585547529371999E-5 34-35 1.0413905617670114 1.2028511082289884E-4 6.501897882318856E-6 4.984788376444456E-5 7.585547529371999E-5 36-37 1.1263140173072719 1.2461970941111142E-4 6.501897882318856E-6 5.09315334114977E-5 7.585547529371999E-5 38-39 1.2504200767856801 1.4304175341101484E-4 6.501897882318856E-6 5.309883270560399E-5 7.802277458782627E-5 40-41 1.414895336565759 1.495436512933337E-4 6.501897882318856E-6 5.8517080940869705E-5 7.802277458782627E-5 42-43 1.6175985049449317 1.549618995285994E-4 6.501897882318856E-6 6.068438023497599E-5 7.802277458782627E-5 44-45 1.8615627172852998 1.6471474635207768E-4 6.501897882318856E-6 6.068438023497599E-5 7.802277458782627E-5 46-47 2.1560824366096383 1.755512428226091E-4 6.501897882318856E-6 6.176802988202914E-5 7.802277458782627E-5 48-49 2.5076172984640306 1.853040896460874E-4 6.501897882318856E-6 6.285167952908228E-5 8.019007388193256E-5 50-51 2.9092146067129843 1.8855503858724681E-4 6.501897882318856E-6 6.393532917613542E-5 8.019007388193256E-5 52-53 3.3501635682450255 1.9722423576367195E-4 6.501897882318856E-6 7.043722705845428E-5 8.019007388193256E-5 54-55 3.8295517500573357 1.9939153505777824E-4 8.669197176425142E-6 7.477182564666685E-5 8.12737235289857E-5 56-57 4.351948902711539 2.1131168117536283E-4 1.0836496470531426E-5 8.12737235289857E-5 8.235737317603884E-5 58-59 4.923035517657486 2.232318272929474E-4 1.0836496470531426E-5 8.777562141130455E-5 8.235737317603884E-5 60-61 5.529182533284191 2.3406832376347882E-4 1.0836496470531426E-5 9.102657035246398E-5 8.235737317603884E-5 62-63 6.135595043074423 2.4057022164579767E-4 1.0836496470531426E-5 9.644481858772969E-5 8.235737317603884E-5 64-65 6.733426131309642 2.4598846988106336E-4 1.0836496470531426E-5 1.018630668229954E-4 8.452467247014513E-5 66-67 7.358890285544716 2.4923941882222284E-4 1.0836496470531426E-5 1.0294671647004855E-4 8.669197176425141E-5 68-69 8.009084408375191 2.514067181163291E-4 1.0836496470531426E-5 1.0836496470531427E-4 8.669197176425141E-5 70-71 8.689241443946683 2.6007591529275426E-4 1.0836496470531426E-5 1.1378321294057998E-4 8.669197176425141E-5 72-73 9.394764550456397 2.6549416352801997E-4 1.0836496470531426E-5 1.1703416188173941E-4 9.319386964657026E-5 74-75 10.144697786801641 2.709124117632857E-4 1.0836496470531426E-5 1.224524101170051E-4 9.536116894067655E-5 76-77 10.941259383809935 2.763306599985514E-4 1.0836496470531426E-5 1.2461970941111142E-4 9.536116894067655E-5 78-79 11.7570460092066 2.8825080611613596E-4 1.0836496470531426E-5 1.2570335905816456E-4 9.644481858772969E-5 80-81 12.592583233070457 2.9041810541024224E-4 1.0836496470531426E-5 1.28954307999324E-4 9.752846823478283E-5 82-83 13.436844920242736 2.9258540470434853E-4 1.0836496470531426E-5 1.3003795764637713E-4 9.752846823478283E-5 84-85 14.289206888526738 2.990873025866674E-4 1.0836496470531426E-5 1.3220525694048341E-4 9.861211788183598E-5 86-87 15.148221381993999 3.0342190117487995E-4 1.0836496470531426E-5 1.3653985552869598E-4 9.969576752888912E-5 88 15.797628775180712 3.077564997630925E-4 1.0836496470531426E-5 1.4087445411690855E-4 9.969576752888912E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 441180 0.0 85.550766 1 AGTGGTA 438215 0.0 85.07289 5 GGTATCA 436390 0.0 84.76483 8 GTGGTAT 442060 0.0 84.025665 6 TGGTATC 440100 0.0 83.982475 7 GCAGTGG 449445 0.0 83.933105 3 GTATCAA 443235 0.0 82.70053 9 AGCAGTG 460345 0.0 82.49065 2 CAGTGGT 457530 0.0 81.98931 4 CAACGCA 433965 0.0 44.445637 12-13 ATCAACG 433750 0.0 44.38172 10-11 ACGCAGA 440870 0.0 44.113804 14-15 AGAGTAC 444620 0.0 43.604393 18-19 AGTACTT 450080 0.0 42.7925 20-21 TCAACGC 433620 0.0 42.098244 12-13 TATCAAC 439560 0.0 41.591446 10-11 GCAGAGT 468530 0.0 41.581394 16-17 AACGCAG 436855 0.0 41.56072 14-15 GAGTACT 432735 0.0 41.326633 20-21 CGCAGAG 442910 0.0 40.962315 16-17 >>END_MODULE