FastQCFastQC Report
Thu 26 May 2016
SRR1660690_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660690_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28326689
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14101134.978036790674689No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1524190.5380755936565689No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528710.18664729930137616No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291300.10283588032473545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1830650.086.06928
GTGGTAT1854100.085.083216
AGTGGTA1864050.084.908845
TGGTATC1855450.084.6780247
GCAGTGG1925700.083.381013
GTATCAA1875450.083.273159
AAGCAGT1935000.083.2354351
AGCAGTG2020650.080.0265662
CAGTGGT1997850.079.6505664
CAACGCA1800100.045.3654812-13
ATCAACG1809400.045.0980410-11
ACGCAGA1843000.044.6199814-15
AGAGTAC1857900.043.94388618-19
TCAACGC1798300.043.32441312-13
AACGCAG1816850.042.73246414-15
TATCAAC1847000.042.17814610-11
CGCAGAG1847750.041.88290416-17
AGTACTT1962050.041.37193320-21
GAGTACT1841650.041.2216120-21
GCAGAGT2036900.040.17038716-17
Produced by FastQC (version 0.11.5)