FastQCFastQC Report
Thu 26 May 2016
SRR1660690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28326689
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12507844.415567241197868No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1397160.49323095967905034No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT628190.22176612310743413No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT305280.10777115532281235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1645800.084.208178
GTGGTAT1672350.083.269966
AGTGGTA1681850.083.0413745
TGGTATC1669650.082.9103557
GTATCAA1688600.081.4221659
GCAGTGG1744050.081.100753
AAGCAGT1749950.080.9493941
AGCAGTG1835350.077.579542
CAGTGGT1825900.077.071984
CAACGCA1611050.044.65236312-13
ATCAACG1614100.044.45969410-11
ACGCAGA1652300.043.88465514-15
AGAGTAC1686000.042.99822218-19
TCAACGC1608050.042.54795512-13
AACGCAG1625950.041.84149614-15
TATCAAC1661400.041.2939710-11
CGCAGAG1660400.040.9330816-17
AGTACTT1783600.040.52412420-21
GAGTACT1663000.040.404320-21
GCAGAGT1868200.038.99934816-17
Produced by FastQC (version 0.11.5)