##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660690_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28326689 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30462112956442 34.0 31.0 34.0 31.0 34.0 2 32.66444786399145 34.0 31.0 34.0 31.0 34.0 3 32.81902579577867 34.0 31.0 34.0 31.0 34.0 4 36.25656140045171 37.0 37.0 37.0 35.0 37.0 5 36.186288273931346 37.0 37.0 37.0 35.0 37.0 6 36.18931344217462 37.0 37.0 37.0 35.0 37.0 7 36.12292749780958 37.0 36.0 37.0 35.0 37.0 8 36.14677687886502 37.0 36.0 37.0 35.0 37.0 9 37.910165356777135 39.0 38.0 39.0 35.0 39.0 10-11 37.83445499048618 39.0 38.0 39.0 35.0 39.0 12-13 37.86758701308155 39.0 38.0 39.0 35.0 39.0 14-15 39.34832694354077 41.0 39.0 41.0 36.0 41.0 16-17 39.28776499434862 41.0 39.0 41.0 36.0 41.0 18-19 39.24019409751701 41.0 39.0 41.0 36.0 41.0 20-21 39.21660110011446 40.5 39.0 41.0 36.0 41.0 22-23 39.087279649944264 40.0 39.0 41.0 35.5 41.0 24-25 39.05302901796959 40.0 39.0 41.0 35.0 41.0 26-27 39.003441983635994 40.0 39.0 41.0 35.0 41.0 28-29 38.89266103426348 40.0 38.5 41.0 35.0 41.0 30-31 38.779677356573515 40.0 38.0 41.0 35.0 41.0 32-33 38.63760146835375 40.0 38.0 41.0 35.0 41.0 34-35 38.53572567199788 40.0 38.0 41.0 34.0 41.0 36-37 38.38550813333673 40.0 38.0 41.0 34.0 41.0 38-39 38.01411029718299 40.0 37.0 41.0 34.0 41.0 40-41 37.89952472030882 40.0 37.0 41.0 33.0 41.0 42-43 37.90784207077643 40.0 37.0 41.0 33.5 41.0 44-45 37.96623415818206 40.0 37.0 41.0 33.0 41.0 46-47 37.865419516555576 40.0 37.0 41.0 33.0 41.0 48-49 37.7539317602562 40.0 37.0 41.0 33.0 41.0 50-51 37.588670899729934 40.0 36.0 41.0 33.0 41.0 52-53 37.194967297448706 40.0 36.0 41.0 32.0 41.0 54-55 36.8327574571105 40.0 36.0 41.0 31.0 41.0 56-57 36.50889193226925 40.0 35.5 41.0 29.5 41.0 58-59 36.29067602994476 39.0 35.0 41.0 29.0 41.0 60-61 36.13935267902295 39.0 35.0 41.0 29.0 41.0 62-63 35.95101554226828 39.0 35.0 41.0 28.0 41.0 64-65 35.70256788571372 38.5 35.0 40.5 28.0 41.0 66-67 35.43806353435801 38.0 35.0 40.0 28.0 41.0 68-69 35.14471320668646 37.0 35.0 40.0 28.0 41.0 70-71 34.785577093037595 37.0 34.5 39.5 27.0 41.0 72-73 33.097993150558466 36.0 33.0 39.0 14.0 41.0 74-75 32.222190493212956 35.0 31.5 38.5 11.0 40.0 76-77 31.84276962266928 34.5 31.0 36.5 22.0 39.0 78-79 33.17162390563895 35.0 33.0 37.0 26.0 39.0 80-81 33.18001415555486 35.0 34.0 37.0 26.0 39.0 82-83 32.923577849144316 35.0 34.0 36.0 25.5 38.5 84-85 32.67188230152843 35.0 34.0 36.0 25.0 37.0 86-87 32.37306003183076 35.0 33.5 35.5 24.5 37.0 88-89 32.18114190825479 35.0 33.0 35.0 24.0 36.5 90-91 32.061033801020656 35.0 33.0 35.0 24.0 36.0 92-93 31.93254610519429 35.0 33.0 35.0 24.0 36.0 94-95 31.804419658788923 35.0 33.0 35.0 23.5 36.0 96-97 31.677145006251877 35.0 33.0 35.0 23.0 35.5 98-99 31.587905879151638 35.0 33.0 35.0 23.0 35.0 100 31.532356958485334 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5637173255291472 1101 2 0.4063093572009464 1101 3 0.3326011609616444 1101 4 0.23258861874501235 1101 5 0.22462508515145885 1101 6 0.21476243992729138 1101 7 0.25259990280191147 1101 8 0.2645127342181226 1101 9 0.3410258025548387 1101 10-11 0.31782729980065483 1101 12-13 0.3230869037987034 1101 14-15 0.37240305124228 1101 16-17 0.423901934465583 1101 18-19 0.42775121945526706 1101 20-21 0.3953478747113124 1101 22-23 0.3809000466331298 1101 24-25 0.41323202072040033 1101 26-27 0.39296106290574073 1101 28-29 0.3757855969642634 1101 30-31 0.35328460415572494 1101 32-33 0.4131394734984113 1101 34-35 0.40385342892276554 1101 36-37 0.38145065454897065 1101 38-39 0.358538612681258 1101 40-41 0.389237757957666 1101 42-43 0.3682837929267251 1101 44-45 0.3791243969027107 1101 46-47 0.34618785552228104 1101 48-49 0.30339494159311897 1101 50-51 0.3092115746875237 1101 52-53 0.22841033001895283 1101 54-55 0.15310026032369706 1101 56-57 0.15716196238385294 1101 58-59 0.18440400045140137 1101 60-61 0.1333771275215625 1101 62-63 0.1443013477362456 1101 64-65 0.16494148718201984 1101 66-67 0.16226647929524773 1101 68-69 0.17057544518426226 1101 70-71 0.18129651158157856 1101 72-73 1.4778667670080452 1101 74-75 2.0180222210249497 1101 76-77 0.6188740066180465 1101 78-79 0.3578655769898731 1101 80-81 0.2553030789017967 1101 82-83 0.22043365306947038 1101 84-85 0.20155759072774515 1101 86-87 0.22603004834731877 1101 88-89 0.214688111671002 1101 90-91 0.17314201213498848 1101 92-93 0.09384703692022 1101 94-95 0.07202232307285428 1101 96-97 0.09868177790045607 1101 98-99 0.08756062167156031 1101 100 0.07872594554396528 1102 1 0.5248979892167185 1102 2 0.381474048884769 1102 3 0.30300522565855204 1102 4 0.19878169478013064 1102 5 0.1940742913206961 1102 6 0.19110514892169306 1102 7 0.22722032803682168 1102 8 0.23868091776206057 1102 9 0.2871851440106141 1102 10-11 0.2658566516117986 1102 12-13 0.2687892090384736 1102 14-15 0.3213625509505107 1102 16-17 0.3314132221330297 1102 18-19 0.35104720308716963 1102 20-21 0.3211377794879269 1102 22-23 0.31867151464861365 1102 24-25 0.34002033638558515 1102 26-27 0.32807105744309695 1102 28-29 0.3111629603237134 1102 30-31 0.27739691215432316 1102 32-33 0.32230435017559955 1102 34-35 0.32466383036920377 1102 36-37 0.30510419427802304 1102 38-39 0.34098624813397294 1102 40-41 0.3494224406520843 1102 42-43 0.30484509772191615 1102 44-45 0.33968348430789774 1102 46-47 0.28216521729125077 1102 48-49 0.20855926156318816 1102 50-51 0.22827851111676267 1102 52-53 0.1959066282439892 1102 54-55 0.1680296833086885 1102 56-57 0.19592908606765747 1102 58-59 0.2909719485003208 1102 60-61 0.2268615999476964 1102 62-63 0.2044242648991812 1102 64-65 0.16660396237951858 1102 66-67 0.1832776895072712 1102 68-69 0.18448285114801166 1102 70-71 0.26037542940335356 1102 72-73 1.563310830996464 1102 74-75 2.0101812045832403 1102 76-77 0.6499021092219657 1102 78-79 0.40753066611220135 1102 80-81 0.22369264985496073 1102 82-83 0.20884115926138946 1102 84-85 0.1977747857842047 1102 86-87 0.2391221842378357 1102 88-89 0.19746049461240744 1102 90-91 0.12462244800280331 1102 92-93 0.09140026277505697 1102 94-95 0.1430547125344006 1102 96-97 0.14958771018284267 1102 98-99 0.14544474164638288 1102 100 0.13503076905503875 1103 1 0.5157839009995158 1103 2 0.37072772704694046 1103 3 0.2730908622673809 1103 4 0.18319843578748163 1103 5 0.20695941084206027 1103 6 0.17088110856687422 1103 7 0.20866774300786517 1103 8 0.21564641392976824 1103 9 0.20161836664212274 1103 10-11 0.22199775725966475 1103 12-13 0.22893752229150977 1103 14-15 0.27940314894151186 1103 16-17 0.3284831225286169 1103 18-19 0.32174859872570494 1103 20-21 0.2902151186027382 1103 22-23 0.2834518318829211 1103 24-25 0.28062362501165694 1103 26-27 0.25549373084444227 1103 28-29 0.31912597798972797 1103 30-31 0.23078974752760217 1103 32-33 0.27637875366536235 1103 34-35 0.26540828971959485 1103 36-37 0.2714940666873318 1103 38-39 0.28964485163480447 1103 40-41 0.3317076535568404 1103 42-43 0.25751554094060936 1103 44-45 0.2591203954245742 1103 46-47 0.22924711107740592 1103 48-49 0.1850364621532563 1103 50-51 0.2088316069259477 1103 52-53 0.120326808736813 1103 54-55 0.11225588907220185 1103 56-57 0.18575731163650033 1103 58-59 0.23719381021182784 1103 60-61 0.18436830913753255 1103 62-63 0.19410736354132752 1103 64-65 0.17288321653450112 1103 66-67 0.14856890513850374 1103 68-69 0.1744838025272415 1103 70-71 0.19066279508722062 1103 72-73 1.5032169535755528 1103 74-75 1.962920037637204 1103 76-77 0.5691378023085356 1103 78-79 0.35748165741457427 1103 80-81 0.26379066101105053 1103 82-83 0.18398708593651492 1103 84-85 0.1817377026403122 1103 86-87 0.22632148733364232 1103 88-89 0.21860339487307812 1103 90-91 0.17073138636946084 1103 92-93 0.14766490025116852 1103 94-95 0.10810482669990762 1103 96-97 0.12144189687158402 1103 98-99 0.11436366723370384 1103 100 0.1154861745528315 1104 1 0.42996133956174987 1104 2 0.31599489874084696 1104 3 0.20882511431481277 1104 4 0.14897959335146282 1104 5 0.1462231994442007 1104 6 0.1258057719424741 1104 7 0.16088764973105896 1104 8 0.1587779203061075 1104 9 0.17922169825229872 1104 10-11 0.1702846745141926 1104 12-13 0.18814125373712187 1104 14-15 0.20412008678228233 1104 16-17 0.2533944625878277 1104 18-19 0.26287283721816834 1104 20-21 0.20671357436802396 1104 22-23 0.22134352869426266 1104 24-25 0.26511604608421635 1104 26-27 0.2158188105406893 1104 28-29 0.23213175164897137 1104 30-31 0.1933836150752981 1104 32-33 0.24973957841807248 1104 34-35 0.2557767694278752 1104 36-37 0.23308659953084288 1104 38-39 0.28115652568462224 1104 40-41 0.28879526653805243 1104 42-43 0.260105975493083 1104 44-45 0.21881549730439787 1104 46-47 0.21626978665332786 1104 48-49 0.19238909794077586 1104 50-51 0.1888234800201758 1104 52-53 0.11417588789009159 1104 54-55 0.09486728041041204 1104 56-57 0.14847062625177188 1104 58-59 0.20993536693094228 1104 60-61 0.17794877067625947 1104 62-63 0.19168112122251557 1104 64-65 0.21261639085921757 1104 66-67 0.17428721233863342 1104 68-69 0.2233228114190169 1104 70-71 0.20621686526853011 1104 72-73 1.5351936393313537 1104 74-75 2.025898738938146 1104 76-77 0.5850249087483412 1104 78-79 0.3549392935923592 1104 80-81 0.25408291265711824 1104 82-83 0.20872460495420597 1104 84-85 0.1576754797484483 1104 86-87 0.23492220269115194 1104 88-89 0.2456336204793459 1104 90-91 0.1744846754124083 1104 92-93 0.11855421769132235 1104 94-95 0.1533424295298218 1104 96-97 0.15053843745311113 1104 98-99 0.17771463178860003 1104 100 0.16980953860451464 1105 1 0.38400097083383855 1105 2 0.24083978272816609 1105 3 0.1783294409005265 1105 4 0.15339905135465415 1105 5 0.12652081923009462 1105 6 0.1444708715693963 1105 7 0.11433673453248616 1105 8 0.13851255588104294 1105 9 0.12629835102632825 1105 10-11 0.15265181283375995 1105 12-13 0.14329270898058866 1105 14-15 0.16150381570294314 1105 16-17 0.18882275316771313 1105 18-19 0.21538026994526405 1105 20-21 0.18232265332625275 1105 22-23 0.15455460461337367 1105 24-25 0.1938783540426101 1105 26-27 0.16830909828941998 1105 28-29 0.1895669593537832 1105 30-31 0.14846139765142397 1105 32-33 0.20779568866409903 1105 34-35 0.1964039536586526 1105 36-37 0.15835993258103542 1105 38-39 0.188951990916145 1105 40-41 0.1909073750271162 1105 42-43 0.18663835256634087 1105 44-45 0.16975161187018983 1105 46-47 0.13522051272468616 1105 48-49 0.09305759549318537 1105 50-51 0.08126852056113165 1105 52-53 0.010506987803402978 1105 54-55 -0.02729023314302026 1105 56-57 0.01826683716770816 1105 58-59 0.08548140348426614 1105 60-61 0.026778245355878028 1105 62-63 0.02753982780835429 1105 64-65 0.06650711075913307 1105 66-67 0.05421846705161926 1105 68-69 0.06186457243943977 1105 70-71 0.08908427112994843 1105 72-73 1.4365527719420328 1105 74-75 1.8841244134586859 1105 76-77 0.43162384492029204 1105 78-79 0.25125968807344634 1105 80-81 0.1732875435176524 1105 82-83 0.12807072416925536 1105 84-85 0.12095381760387625 1105 86-87 0.17345423548673722 1105 88-89 0.1414215872728377 1105 90-91 0.06787846530956898 1105 92-93 0.04083028654635612 1105 94-95 0.04955870757488867 1105 96-97 0.05413731060771099 1105 98-99 0.03620619441713657 1105 100 0.020743191359382962 1106 1 0.33022654446295974 1106 2 0.2046190694534502 1106 3 0.13617881636901075 1106 4 0.09161182646514021 1106 5 0.0810508493031179 1106 6 0.07552416958911579 1106 7 0.08907785856574435 1106 8 0.10433317443377632 1106 9 0.11008046354210421 1106 10-11 0.10634776531900059 1106 12-13 0.11323655284307677 1106 14-15 0.08330574708924132 1106 16-17 0.12794608826457932 1106 18-19 0.15698607023441014 1106 20-21 0.1024772026715226 1106 22-23 0.0861520911969933 1106 24-25 0.11594155059090383 1106 26-27 0.10610140187679207 1106 28-29 0.09986267305173158 1106 30-31 0.11466142329066997 1106 32-33 0.15723775281671948 1106 34-35 0.15850732482460472 1106 36-37 0.1142923695304674 1106 38-39 0.16870681057991987 1106 40-41 0.18174956835012068 1106 42-43 0.1679452856423822 1106 44-45 0.15549810245692441 1106 46-47 0.12461027369766242 1106 48-49 0.072471419809645 1106 50-51 0.03499554731457266 1106 52-53 0.0068710679814358855 1106 54-55 -0.046335329116658386 1106 56-57 -0.035366882329505245 1106 58-59 0.05379040538932145 1106 60-61 0.03113835680530741 1106 62-63 0.02663273426637147 1106 64-65 0.04472134760277413 1106 66-67 0.06451418271357312 1106 68-69 0.10053804226675567 1106 70-71 0.0997465834187139 1106 72-73 1.4645280048088622 1106 74-75 1.9292445520420856 1106 76-77 0.4136083883360122 1106 78-79 0.23585423630368751 1106 80-81 0.11296564196150882 1106 82-83 0.11350011659850168 1106 84-85 0.11574355460830077 1106 86-87 0.20855844924418676 1106 88-89 0.2027656539226328 1106 90-91 0.13103657436263205 1106 92-93 0.08480240420896124 1106 94-95 0.1272283689483764 1106 96-97 0.1343202148790219 1106 98-99 0.10518861223140519 1106 100 0.13882813395106908 1107 1 0.3270226364675466 1107 2 0.2133492185486503 1107 3 0.12233687820521055 1107 4 0.08920334930631668 1107 5 0.09096701624680037 1107 6 0.07420985923558732 1107 7 0.0778907385824894 1107 8 0.08003757657402844 1107 9 0.08636702744055924 1107 10-11 0.09259935041757217 1107 12-13 0.10278592659160068 1107 14-15 0.08403454434915858 1107 16-17 0.11274192975857034 1107 18-19 0.11365382012542113 1107 20-21 0.07393648299144928 1107 22-23 0.08147979230300706 1107 24-25 0.08828539598306406 1107 26-27 0.05712264318481175 1107 28-29 0.06911758413844638 1107 30-31 0.08867634546236047 1107 32-33 0.12858380565231897 1107 34-35 0.10217106006408727 1107 36-37 0.09882316146283898 1107 38-39 0.17619067421973256 1107 40-41 0.14790473809359383 1107 42-43 0.07740088454952598 1107 44-45 0.11336048773687679 1107 46-47 0.10951854011109674 1107 48-49 0.08714401639250013 1107 50-51 0.03966191600645885 1107 52-53 0.012538051374065162 1107 54-55 -0.010889201335416487 1107 56-57 -0.04215136459347235 1107 58-59 -0.0011747330667688516 1107 60-61 -0.047951876481512556 1107 62-63 -0.04053984058766247 1107 64-65 -0.009025829041362954 1107 66-67 -0.008936220207921508 1107 68-69 -0.002743907514371813 1107 70-71 0.029177157073618787 1107 72-73 1.4410144399681357 1107 74-75 1.8795911704740078 1107 76-77 0.3994307692575809 1107 78-79 0.23002092510045458 1107 80-81 0.15142127583164466 1107 82-83 0.11379918550623813 1107 84-85 0.08876021647857613 1107 86-87 0.1540988733709554 1107 88-89 0.12005332868250207 1107 90-91 0.07600223663025929 1107 92-93 0.038100670698529626 1107 94-95 0.04239728620323646 1107 96-97 0.046895174705856135 1107 98-99 0.047900920592592655 1107 100 0.06172293643816218 1108 1 0.3089547148267755 1108 2 0.16833110438440713 1108 3 0.09867900478309366 1108 4 0.056853347660528186 1108 5 0.03916092619362388 1108 6 0.05374563985638048 1108 7 0.0655687314493889 1108 8 0.024380016705208618 1108 9 -0.006961321558875966 1108 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-1.4618229307729678 2316 52-53 -1.250955357370927 2316 54-55 -1.2987721303193283 2316 56-57 -1.1382875366981224 2316 58-59 -0.9815868593133246 2316 60-61 -0.9295609245760872 2316 62-63 -0.9873661825931279 2316 64-65 -1.1380314503623339 2316 66-67 -0.979268654657119 2316 68-69 -0.9888193422258027 2316 70-71 -2.270682143615673 2316 72-73 -0.5122606376184464 2316 74-75 0.8245940045400246 2316 76-77 -0.29940629582375067 2316 78-79 -1.820964988401066 2316 80-81 -1.624686460306176 2316 82-83 -0.88048136739787 2316 84-85 -0.7616426147864814 2316 86-87 -0.7042976915612869 2316 88-89 -0.8329296255222793 2316 90-91 -0.9546587775010913 2316 92-93 -0.8574726931123813 2316 94-95 -0.8997765876633963 2316 96-97 -0.9063857404501228 2316 98-99 -0.9192597887262401 2316 100 -0.9556236377151706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 21.0 7 602.0 8 1127.0 9 1433.0 10 5062.0 11 7145.0 12 5203.0 13 5240.0 14 7829.0 15 14634.0 16 24658.0 17 36843.0 18 49504.0 19 66723.0 20 99679.0 21 105375.0 22 120895.0 23 185335.0 24 299252.0 25 410328.0 26 427606.0 27 370966.0 28 344096.0 29 372353.0 30 432054.0 31 516982.0 32 636734.0 33 815641.0 34 1140877.0 35 1831830.0 36 3126581.0 37 6161199.0 38 8150404.0 39 2547543.0 40 4933.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.00965444637983 35.22314675805587 27.525501577001393 20.241697218562912 2 20.11350931099791 32.524339347702195 29.391450027135214 17.970701314164682 3 23.62197713965088 27.962374988478178 27.726731493398326 20.688916378472612 4 18.80226529854598 28.263443158330414 27.970530134763077 24.963761408360526 5 19.992356966721918 32.93903754265007 27.441119519036093 19.62748597159192 6 24.99411420798244 27.592490601354786 27.885182768801535 19.528212421861234 7 19.757674467354796 28.025114407123258 32.75965291954877 19.45755820597317 8 24.32299447351577 27.579305156349193 28.27752301019014 19.82017735994489 9 24.254666685541682 27.255956387984494 28.77947366174705 19.709903264726776 10-11 19.333475578455356 30.572865399129423 30.52207054626116 19.571588476154062 12-13 19.390611660931352 28.054608936291782 30.1689528945015 22.38582650827537 14-15 19.344098578723333 33.36961950059768 27.484203452642465 19.80207846803652 16-17 22.18987598970584 27.964515764784398 27.446025019313865 22.3995832261959 18-19 19.695914690206116 30.653489011723185 27.465975638734196 22.184620659336503 20-21 22.221347507292506 30.63910152012471 27.473240871885874 19.666310100696908 22-23 22.07510203539849 28.158449792702566 30.127199123060233 19.63924904883871 24-25 19.56561790431312 30.527609528310162 27.444602064837543 22.462170502539173 26-27 19.53255926525832 27.987096059947646 32.780098812730515 19.700245862063518 28-29 19.585273204895913 28.092835355006628 32.66912992891537 19.65276151118209 30-31 19.637942071310878 28.051096896780088 32.660869555385474 19.650091476523556 32-33 19.669110609278853 28.143804594082557 32.51756076731397 19.669524029324627 34-35 19.717413501780932 28.14750932163515 32.48115132950026 19.653925847083663 36-37 19.712413133580622 28.13964854793839 32.4896647337887 19.658273584692285 38-39 19.74064587230126 28.187889995504555 32.45484187254417 19.616622259650008 40-41 19.750343048573008 28.32908212082922 32.3558638323633 19.564710998234478 42-43 19.763792138918753 28.296846370857804 32.315869365290375 19.623492124933072 44-45 19.802885846789934 28.35416062419994 32.22650883190247 19.616444697107653 46-47 19.862981971161364 28.459352247379964 32.143809252146056 19.53385652931262 48-49 19.886388035564384 28.547449639382943 32.00671233378787 19.559449991264806 50-51 19.969939814764658 28.581130023512717 31.808866229347803 19.640063932374815 52-53 20.036782023495547 28.72773240581236 31.570053692778753 19.66543187791334 54-55 20.24560996834103 28.9224413330057 31.03975164455759 19.792197054095684 56-57 20.58418407069048 29.393576742505513 30.14592053037618 19.876318656427824 58-59 20.774234639630702 29.74876682989043 29.53438820989241 19.94261032058645 60-61 20.78238964744183 29.98356456639506 29.224889426284644 20.009156359878467 62-63 20.72043265263482 30.12043955217547 29.156408773129534 20.002719022060173 64-65 20.824989715278804 30.26248998903264 28.89053593385561 20.02198436183294 66-67 20.735591719910897 30.243507235979468 28.927182168071475 20.093718876038164 68-69 20.746278244647616 30.099239715069093 29.022574364163766 20.13190767611953 70-71 20.713296411912317 30.016530671776575 29.089226996074448 20.180945920236663 72-73 21.303982066443275 29.782764909544408 28.793180536231404 20.120072487780913 74-75 20.6893496961537 29.994854962662483 28.913022730033443 20.402772611150375 76-77 20.738464159161886 30.07297489388302 28.739533412545303 20.449027534409787 78-79 20.82600374391114 30.2006342784123 28.4236201984164 20.549741779260163 80-81 20.850553681558438 30.29079723902391 28.201083596228475 20.65756548318918 82-83 20.897199461776005 30.358934830104307 27.95263661492193 20.79122909319776 84-85 20.89206315834602 30.334069369641647 27.837012544818386 20.936854927193945 86-87 20.934763425841965 30.2889313701668 27.716963565006104 21.05934163898513 88-89 20.953332521727482 30.242892205689298 27.682561300362842 21.121213972220378 90-91 20.979703761626254 30.152372947923787 27.61954283150833 21.248380458941632 92-93 21.010596902879094 30.043101781572574 27.63629621359915 21.310005101949184 94-95 21.023590888739434 29.977264808064298 27.638598973472334 21.360545329723934 96-97 21.01790469267368 29.878418769964117 27.63477144407394 21.468905093288257 98-99 21.014464407794183 29.78228079634481 27.63568745258187 21.567567343279137 100 20.96671133933273 29.75866298206129 27.63111440492572 21.643511273680257 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 140052.0 1 86973.5 2 26059.5 3 16670.0 4 14835.5 5 14392.5 6 14637.5 7 15190.0 8 15297.0 9 15578.0 10 17136.5 11 24024.0 12 164960.5 13 192931.5 14 76839.0 15 70910.0 16 78762.5 17 87794.0 18 96335.0 19 105634.5 20 119460.5 21 142805.0 22 175183.0 23 210750.5 24 245208.0 25 282263.0 26 324403.5 27 373743.0 28 439824.0 29 509217.0 30 563539.5 31 617845.0 32 678675.0 33 723700.0 34 754900.5 35 802372.5 36 855277.0 37 890275.5 38 922468.0 39 943400.5 40 946508.0 41 955321.0 42 981270.5 43 987720.0 44 972123.5 45 959974.0 46 943812.0 47 928386.5 48 901307.0 49 868474.0 50 841139.5 51 808695.5 52 765646.5 53 706955.0 54 638139.5 55 571079.5 56 509521.5 57 451503.0 58 393422.0 59 330806.0 60 268876.5 61 217205.0 62 168608.0 63 122480.0 64 86826.5 65 59885.5 66 41295.5 67 30657.5 68 22640.5 69 16131.5 70 11686.0 71 8791.5 72 7027.5 73 5613.5 74 4895.0 75 4750.5 76 3488.0 77 1586.5 78 706.0 79 457.5 80 296.0 81 196.0 82 135.0 83 95.0 84 71.5 85 57.0 86 34.0 87 20.5 88 14.0 89 15.0 90 12.0 91 6.5 92 5.5 93 2.5 94 1.5 95 1.5 96 2.5 97 2.5 98 2.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20422083216291181 2 9.178623029327572E-5 3 0.0 4 0.0018816177210121523 5 8.437272707728037E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.294655757331893E-5 14-15 3.000703682664783E-5 16-17 0.0018798526011988201 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005660739241356447 26-27 0.03170684720688676 28-29 0.055984305119458184 30-31 0.08787472478693151 32-33 0.09240225710812866 34-35 0.09981399520431068 36-37 0.10720984722217271 38-39 0.09900733544961784 40-41 0.059740480082229167 42-43 0.042394647676613384 44-45 0.04658857235309075 46-47 0.08778293855663823 48-49 0.11685799212184664 50-51 0.13100542742570442 52-53 0.49072625466393194 54-55 0.47600515542074123 56-57 0.48217601428815066 58-59 0.4748595926618886 60-61 0.49325743647625037 62-63 0.4815476316346044 64-65 0.46153470319104356 66-67 0.46767025966218645 68-69 0.4771224762625805 70-71 0.48460305403148246 72-73 1.1697449002952658 74-75 3.1644114848720934 76-77 0.4833798260008433 78-79 0.4937234281069701 80-81 0.5179832348214082 82-83 0.5293400157003877 84-85 0.5282544670151884 86-87 0.5012428385117653 88-89 0.47892995895143264 90-91 0.45227311953048943 92-93 0.4403003118366569 94-95 0.44373523499340145 96-97 0.46337748827616243 98-99 0.45683418912813983 100 0.4579779867671792 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8326689E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.26000310584241 #Duplication Level Percentage of deduplicated Percentage of total 1 74.71205614563073 28.584835001757043 2 12.3239225673942 9.430266314093268 3 4.345971684192755 4.98830670405354 4 2.183492796037747 3.3416176463195493 5 1.2929067662996288 2.4733308447094227 6 0.9012056358345021 2.0688078255618443 7 0.6250556356282908 1.6740241392323898 8 0.4886105741248937 1.49553936668527 9 0.38125041807303506 1.3127977961620236 >10 2.3231913410151206 16.63274242266634 >50 0.20534798286396275 5.418549999795316 >100 0.21217304043311003 15.70088536860463 >500 0.004084834224028601 0.9045347562633332 >1k 6.105869193688216E-4 0.47379175150692326 >5k 7.384081760067702E-5 0.20676919052156767 >10k+ 4.6150510994365694E-5 5.293200872067421 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1250784 4.415567241197868 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139716 0.49323095967905034 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62819 0.22176612310743413 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 30528 0.10777115532281235 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.09816890353828504 3.530239626664451E-6 3.530239626664451E-6 3.530239626664451E-6 2.1181437759986703E-5 2 0.09817596401753838 1.0590718879993352E-5 3.530239626664451E-6 3.530239626664451E-6 2.4711677386651153E-5 3 0.09817596401753838 1.4120958506657803E-5 3.530239626664451E-6 3.530239626664451E-6 2.4711677386651153E-5 4 0.09817596401753838 1.7651198133322253E-5 3.530239626664451E-6 3.530239626664451E-6 2.8241917013315607E-5 5 0.09819008497604503 2.1181437759986703E-5 3.530239626664451E-6 7.060479253328902E-6 2.8241917013315607E-5 6 0.09819008497604503 2.4711677386651153E-5 3.530239626664451E-6 1.0590718879993352E-5 3.177215663998005E-5 7 0.09819714545529835 3.177215663998005E-5 3.530239626664451E-6 1.4120958506657803E-5 3.5302396266644506E-5 8 0.09820067569492502 3.177215663998005E-5 3.530239626664451E-6 1.7651198133322253E-5 3.5302396266644506E-5 9 0.09820067569492502 3.883263589330896E-5 3.530239626664451E-6 1.7651198133322253E-5 3.5302396266644506E-5 10-11 0.09820950129399168 4.2362875519973406E-5 3.530239626664451E-6 1.7651198133322253E-5 3.706751607997673E-5 12-13 0.098216561773245 4.2362875519973406E-5 3.530239626664451E-6 2.1181437759986703E-5 3.883263589330896E-5 14-15 0.09823244785156501 4.589311514663786E-5 3.530239626664451E-6 2.1181437759986703E-5 3.883263589330896E-5 16-17 0.09825009904969832 4.9423354773302306E-5 3.530239626664451E-6 2.4711677386651153E-5 4.059775570664118E-5 18-19 0.09827304560727165 5.471871421329898E-5 3.530239626664451E-6 2.8241917013315607E-5 4.2362875519973406E-5 20-21 0.0982871665657783 7.060479253328901E-5 3.530239626664451E-6 3.000703682664783E-5 4.765823495997008E-5 22-23 0.09835424111868493 8.119551141328237E-5 3.530239626664451E-6 3.883263589330896E-5 5.295359439996676E-5 24-25 0.09868078828415139 8.825599066661127E-5 3.530239626664451E-6 3.883263589330896E-5 5.295359439996676E-5 26-27 0.10003287006116388 9.708158973327239E-5 3.530239626664451E-6 3.883263589330896E-5 5.471871421329899E-5 28-29 0.1047280887646276 1.0237694917326907E-4 3.530239626664451E-6 3.883263589330896E-5 6.35443132799601E-5 30-31 0.11735222566957967 1.0943742842659798E-4 3.530239626664451E-6 4.059775570664118E-5 6.707455290662457E-5 32-33 0.14480866436596243 1.2355838693325577E-4 3.530239626664451E-6 4.589311514663786E-5 7.236991234662124E-5 34-35 0.194366168245078 1.2885374637325244E-4 3.530239626664451E-6 4.589311514663786E-5 7.413503215995346E-5 36-37 0.27427843755406783 1.4120958506657803E-4 3.530239626664451E-6 4.589311514663786E-5 7.590015197328568E-5 38-39 0.3889882788630892 1.4297470487991025E-4 3.530239626664451E-6 4.9423354773302306E-5 9.355135010660794E-5 40-41 0.5446259532838448 1.5180030394657137E-4 3.530239626664451E-6 5.648383402663121E-5 1.0061182935993684E-4 42-43 0.7442151110565729 1.7651198133322252E-4 3.530239626664451E-6 5.8248953839963436E-5 1.0237694917326907E-4 44-45 0.9913583617202844 1.8180734077321922E-4 3.530239626664451E-6 6.001407365329566E-5 1.0237694917326907E-4 46-47 1.2880997140188182 1.9416317946654478E-4 3.530239626664451E-6 6.001407365329566E-5 1.041420689866013E-4 48-49 1.634545075140974 2.082841379732026E-4 3.530239626664451E-6 6.001407365329566E-5 1.0590718879993352E-4 50-51 2.031024522491845 2.1181437759986704E-4 3.530239626664451E-6 6.707455290662457E-5 1.0590718879993352E-4 52-53 2.47440143816314 2.1887485685319593E-4 3.530239626664451E-6 6.707455290662457E-5 1.0590718879993352E-4 54-55 2.9650500275552854 2.294655757331893E-4 3.530239626664451E-6 6.707455290662457E-5 1.0590718879993352E-4 56-57 3.502910276594628 2.3476093517318597E-4 3.530239626664451E-6 7.060479253328901E-5 1.0590718879993352E-4 58-59 4.084651404193409 2.4182141442651486E-4 3.530239626664451E-6 7.060479253328901E-5 1.0767230861326574E-4 60-61 4.700544069940543 2.4711677386651153E-4 3.530239626664451E-6 7.413503215995346E-5 1.1473278786659465E-4 62-63 5.323364830955005 2.541772531198404E-4 3.530239626664451E-6 7.413503215995346E-5 1.1649790767992687E-4 64-65 5.946040852144774 2.647679719998338E-4 3.530239626664451E-6 7.766527178661792E-5 1.2885374637325244E-4 66-67 6.602377708174789 2.788889305064916E-4 3.530239626664451E-6 8.119551141328237E-5 1.3061886618658469E-4 68-69 7.280963899451856 2.877145295731527E-4 3.530239626664451E-6 8.825599066661126E-5 1.3061886618658469E-4 70-71 7.990407915305598 2.983052484531461E-4 3.530239626664451E-6 8.825599066661126E-5 1.3061886618658469E-4 72-73 8.734935452569129 3.0889596733313943E-4 3.530239626664451E-6 9.00211104799435E-5 1.3767934543991358E-4 74-75 9.535581444057934 3.141913267731361E-4 3.530239626664451E-6 9.178623029327572E-5 1.3767934543991358E-4 76-77 10.38784836448764 3.2654716546646166E-4 3.530239626664451E-6 9.178623029327572E-5 1.3767934543991358E-4 78-79 11.265854262035354 3.3890300415978725E-4 3.530239626664451E-6 9.178623029327572E-5 1.3767934543991358E-4 80-81 12.163633031732019 3.4066812397311945E-4 3.530239626664451E-6 9.178623029327572E-5 1.3767934543991358E-4 82-83 13.081551818498802 3.5655420229310954E-4 3.530239626664451E-6 9.178623029327572E-5 1.3767934543991358E-4 84-85 14.015808554257788 3.60084441919774E-4 3.530239626664451E-6 9.531646991994017E-5 1.394444652532458E-4 86-87 14.964272739394286 3.7420540042643177E-4 3.530239626664451E-6 9.531646991994017E-5 1.4120958506657803E-4 88 15.688564943117779 3.7420540042643177E-4 3.530239626664451E-6 9.531646991994017E-5 1.4120958506657803E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 164580 0.0 84.20817 8 GTGGTAT 167235 0.0 83.26996 6 AGTGGTA 168185 0.0 83.041374 5 TGGTATC 166965 0.0 82.910355 7 GTATCAA 168860 0.0 81.422165 9 GCAGTGG 174405 0.0 81.10075 3 AAGCAGT 174995 0.0 80.949394 1 AGCAGTG 183535 0.0 77.57954 2 CAGTGGT 182590 0.0 77.07198 4 CAACGCA 161105 0.0 44.652363 12-13 ATCAACG 161410 0.0 44.459694 10-11 ACGCAGA 165230 0.0 43.884655 14-15 AGAGTAC 168600 0.0 42.998222 18-19 TCAACGC 160805 0.0 42.547955 12-13 AACGCAG 162595 0.0 41.841496 14-15 TATCAAC 166140 0.0 41.29397 10-11 CGCAGAG 166040 0.0 40.93308 16-17 AGTACTT 178360 0.0 40.524124 20-21 GAGTACT 166300 0.0 40.4043 20-21 GCAGAGT 186820 0.0 38.999348 16-17 >>END_MODULE