FastQCFastQC Report
Thu 26 May 2016
SRR1660689_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660689_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26255465
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20027487.627928128486775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2044830.7788207140875242No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1845130.702760358652951Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT629050.239588215253472No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT374560.14265982339295838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2552850.087.3177955
GGTATCA2548200.087.087648
AAGCAGT2631900.086.5516741
TGGTATC2561100.086.4612357
GTGGTAT2575000.086.404236
GCAGTGG2628950.086.0616763
GTATCAA2583400.085.0956049
CAGTGGT2647250.084.6384664
AGCAGTG2705800.084.307162
CAACGCA2527500.045.31665412-13
ATCAACG2541950.045.12572510-11
ACGCAGA2564500.044.95417814-15
AGAGTAC2575550.044.4619318-19
AGTACTT2614250.043.5900520-21
TCAACGC2533600.043.33885612-13
AACGCAG2540950.042.97392714-15
TATCAAC2563400.042.7823210-11
CGCAGAG2578300.042.2360416-17
GAGTACT2536300.042.1464620-21
GCAGAGT2741800.041.84956716-17
Produced by FastQC (version 0.11.5)